2XMyc-LRRK2-WT

LOCUS       2XMyc-LRRK2-WT               11985 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    2XMyc-LRRK2-WT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11985)
  TITLE     The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 
            (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16906-14.
  PUBMED    18397888
REFERENCE   2  (bases 1 to 11985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11985
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        68..371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        372..575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     526..546
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        621..639
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      671..680
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             680..709
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             725..754
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    773..797
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8414..8438)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(8487..8506)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8488..8506)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    8780..8901
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8817..8836)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8871..8890
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8908..9363)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9045..9066)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9257..9276
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9390..9494
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9496..9853
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(9519..9539)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9704..9839
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9766..9785
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9888..10682
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9942..9961)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10552..10571
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10870..10889)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10914..10961
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11290..11878
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11779..11798
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
       61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      601 agcgattacg ccaagctcga aattaaccct cactaaaggg aacaaaagct ggagctccac
      661 cgcggtggcg gccgccatgg agcagaaact catctctgaa gaggatctga gcccgggcgg
      721 atccgagcag aaactcatct ctgaagagga tctgctgcag gaattcgata tcacaagttt
      781 gtacaaaaaa gcaggctccg aattcgccct tatggctagt ggcagctgtc aggggtgcga
      841 agaggacgag gaaactctga agaagttgat agtcaggctg aacaatgtcc aggaaggaaa
      901 acagatagaa acgctggtcc aaatcctgga ggatctgctg gtgttcacgt actccgagca
      961 cgcctccaag ttatttcaag gcaaaaatat ccatgtgcct ctgttgatcg tcttggactc
     1021 ctatatgaga gtcgcgagtg tgcagcaggt gggttggtca cttctgtgca aattaataga
     1081 agtctgtcca ggtacaatgc aaagcttaat gggaccccag gatgttggaa atgattggga
     1141 agtccttggt gttcaccaat tgattcttaa aatgctaaca gttcataatg ccagtgtaaa
     1201 cttgtcagtg attggactga agaccttaga tctcctccta acttcaggta aaatcacctt
     1261 gctgatattg gatgaagaaa gtgatatttt catgttaatt tttgatgcca tgcactcatt
     1321 tccagccaat gatgaagtcc agaaacttgg atgcaaagct ttacatgtgc tgtttgagag
     1381 agtctcagag gagcaactga ctgaatttgt tgagaacaaa gattatatga tattgttaag
     1441 tgcgttaaca aattttaaag atgaagagga aattgtgctt catgtgctgc attgtttaca
     1501 ttccctagcg attccttgca ataatgtgga agtcctcatg agtggcaatg tcaggtgtta
     1561 taatattgtg gtggaagcta tgaaagcatt ccctatgagt gaaagaattc aagaagtgag
     1621 ttgctgtttg ctccataggc ttacattagg taattttttc aatatcctgg tattaaacga
     1681 agtccatgag tttgtggtga aagctgtgca gcagtaccca gagaatgcag cattgcagat
     1741 ctcagcgctc agctgtttgg ccctcctcac tgagactatt ttcttaaatc aagatttaga
     1801 ggaaaagaat gagaatcaag agaatgatga tgagggggaa gaagataaat tgttttggct
     1861 ggaagcctgt tacaaagcat taacgtggca tagaaagaac aagcacgtgc aggaggccgc
     1921 atgctgggca ctaaataatc tccttatgta ccaaaacagt ttacatgaga agattggaga
     1981 tgaagatggc catttcccag ctcataggga agtgatgctc tccatgctga tgcattcttc
     2041 atcaaaggaa gttttccagg catctgcgaa tgcattgtca actctcttag aacaaaatgt
     2101 taatttcaga aaaatactgt tatcaaaagg aatacacctg aatgttttgg agttaatgca
     2161 gaagcatata cattctcccg aagtggctga aagtggctgt aaaatgctaa atcatctttt
     2221 tgaaggaagc aacacttccc tggatataat ggcagcagtg gtccccaaaa tactaacagt
     2281 tatgaaacgt catgagacat cattaccagt gcagctggag gcgcttcgag ctattttaca
     2341 ttttatagtg cctggcatgc cagaagaatc cagggaggat acagaatttc atcataagct
     2401 aaatatggtt aaaaaacagt gtttcaagaa tgatattcac aaactggtcc tagcagcttt
     2461 gaacaggttc attggaaatc ctgggattca gaaatgtgga ttaaaagtaa tttcttctat
     2521 tgtacatttt cctgatgcat tagagatgtt atccctggaa ggtgctatgg attcagtgct
     2581 tcacacactg cagatgtatc cagatgacca agaaattcag tgtctgggtt taagtcttat
     2641 aggatacttg attacaaaga agaatgtgtt cataggaact ggacatctgc tggcaaaaat
     2701 tctggtttcc agcttatacc gatttaagga tgttgctgaa atacagacta aaggatttca
     2761 gacaatctta gcaatcctca aattgtcagc atctttttct aagctgctgg tgcatcattc
     2821 atttgactta gtaatattcc atcaaatgtc ttccaatatc atggaacaaa aggatcaaca
     2881 gtttctaaac ctctgttgca agtgttttgc aaaagtagct atggatgatt acttaaaaaa
     2941 tgtgatgcta gagagagcgt gtgatcagaa taacagcatc atggttgaat gcttgcttct
     3001 attgggagca gatgccaatc aagcaaagga gggatcttct ttaatttgtc aggtatgtga
     3061 gaaagagagc agtcccaaat tggtggaact cttactgaat agtggatctc gtgaacaaga
     3121 tgtacgaaaa gcgttgacga taagcattgg gaaaggtgac agccagatca tcagcttgct
     3181 cttaaggagg ctggccctgg atgttgccaa caatagcatt tgccttggag gattttgtat
     3241 aggaaaagtt gaaccttctt ggcttggtcc tttatttcca gataagactt ctaatttaag
     3301 gaaacaaaca aatatagcat ctacactagc aagaatggtg atcagatatc agatgaaaag
     3361 tgctgtggaa gaaggaacag cctcaggcag cgatggaaat ttttctgaag atgtgctgtc
     3421 taaatttgat gaatggacct ttattcctga ctcttctatg gacagtgtgt ttgctcaaag
     3481 tgatgacctg gatagtgaag gaagtgaagg ctcatttctt gtgaaaaaga aatctaattc
     3541 aattagtgta ggagaatttt accgagatgc cgtattacag cgttgctcac caaatttgca
     3601 aagacattcc aattccttgg ggcccatttt tgatcatgaa gatttactga agcgaaaaag
     3661 aaaaatatta tcttcagatg attcactcag gtcatcaaaa cttcaatccc atatgaggca
     3721 ttcagacagc atttcttctc tggcttctga gagagaatat attacatcac tagacctttc
     3781 agcaaatgaa ctaagagata ttgatgccct aagccagaaa tgctgtataa gtgttcattt
     3841 ggagcatctt gaaaagctgg agcttcacca gaatgcactc acgagctttc cacaacagct
     3901 atgtgaaact ctgaagagtt tgacacattt ggacttgcac agtaataaat ttacatcatt
     3961 tccttcttat ttgttgaaaa tgagttgtat tgctaatctt gatgtctctc gaaatgacat
     4021 tggaccctca gtggttttag atcctacagt gaaatgtcca actctgaaac agtttaacct
     4081 gtcatataac cagctgtctt ttgtacctga gaacctcact gatgtggtag agaaactgga
     4141 gcagctcatt ttagaaggaa ataaaatatc agggatatgc tcccccttga gactgaagga
     4201 actgaagatt ttaaacctta gtaagaacca catttcatcc ctatcagaga actttcttga
     4261 ggcttgtcct aaagtggaga gtttcagtgc cagaatgaat tttcttgctg ctatgccttt
     4321 cttgcctcct tctatgacaa tcctaaaatt atctcagaac aaattttcct gtattccaga
     4381 agcaatttta aatcttccac acttgcggtc tttagatatg agcagcaatg atattcagta
     4441 cctaccaggt cccgcacact ggaaatcttt gaacttaagg gaactcttat ttagccataa
     4501 tcagatcagc atcttggact tgagtgaaaa agcatattta tggtctagag tagagaaact
     4561 gcatctttct cacaataaac tgaaagagat tcctcctgag attggctgtc ttgaaaatct
     4621 gacatctctg gatgtcagtt acaacttgga actaagatcc tttcccaatg aaatggggaa
     4681 attaagcaaa atatgggatc ttcctttgga tgaactgcat cttaactttg attttaaaca
     4741 tataggatgt aaagccaaag acatcataag gtttcttcaa cagcgattaa aaaaggctgt
     4801 gccttataac cgaatgaaac ttatgattgt gggaaatact gggagtggta aaaccacctt
     4861 attgcagcaa ttaatgaaaa ccaagaaatc agatcttgga atgcaaagtg ccacagttgg
     4921 catagatgtg aaagactggc ctatccaaat aagagacaaa agaaagagag atctcgtcct
     4981 aaatgtgtgg gattttgcag gtcgtgagga attctatagt actcatcccc attttatgac
     5041 gcagcgagca ttgtaccttg ctgtctatga cctcagcaag ggacaggctg aagttgatgc
     5101 catgaagcct tggctcttca atataaaggc tcgcgcttct tcttcccctg tgattctcgt
     5161 tggcacacat ttggatgttt ctgatgagaa gcaacgcaaa gcctgcatga gtaaaatcac
     5221 caaggaactc ctgaataagc gagggttccc tgccatacga gattaccact ttgtgaatgc
     5281 caccgaggaa tctgatgctt tggcaaaact tcggaaaacc atcataaacg agagccttaa
     5341 tttcaagatc cgagatcagc ttgttgttgg acagctgatt ccagactgct atgtagaact
     5401 tgaaaaaatc attttatcgg agcgtaaaaa tgtgccaatt gaatttcccg taattgaccg
     5461 gaaacgatta ttacaactag tgagagaaaa tcagctgcag ttagatgaaa atgagcttcc
     5521 tcacgcagtt cactttctaa atgaatcagg agtccttctt cattttcaag acccagcact
     5581 gcagttaagt gacttgtact ttgtggaacc caagtggctt tgtaaaatca tggcacagat
     5641 tttgacagtg aaagtggaag gttgtccaaa acaccctaag ggaattattt cgcgtagaga
     5701 tgtggaaaaa tttctttcaa aaaaaaggaa atttccaaag aactacatgt cacagtattt
     5761 taagctccta gaaaaattcc agattgcttt gccaatagga gaagaatatt tgctggttcc
     5821 aagcagtttg tctgaccaca ggcctgtgat agagcttccc cattgtgaga actctgaaat
     5881 tatcatccga ctatatgaaa tgccttattt tccaatggga ttttggtcaa gattaatcaa
     5941 tcgattactt gagatttcac cttacatgct ttcagggaga gaacgagcac ttcgcccaaa
     6001 cagaatgtat tggcgacaag gcatttactt aaattggtct cctgaagctt attgtctggt
     6061 aggatctgaa gtcttagaca atcatccaga gagtttctta aaaattacag ttccttcttg
     6121 tagaaaaggc tgtattcttt tgggccaagt tgtggaccac attgattctc tcatggaaga
     6181 atggtttcct gggttgctgg agattgatat ttgtggtgaa ggagaaactc tgttgaagaa
     6241 atgggcatta tatagtttta atgatggcga agaacatcaa aaaatcttac ttgatgactt
     6301 gatgaagaaa gcagaggaag gagatctctt agtaaatcca gatcaaccaa ggctcaccat
     6361 tccaatatct cagattgccc ctgacttgat tttggctgac ctgcctagaa atattatgtt
     6421 gaataatgat gagttggaat ttgaacaagc tccagagttt ctcctaggtg atggcagttt
     6481 tggatcagtt taccgagcag cctatgaagg agaagaagtg gctgtgaaga tttttaataa
     6541 acatacatca ctcaggctgt taagacaaga gcttgtggtg ctttgccacc tccaccaccc
     6601 cagtttgata tctttgctgg cagctgggat tcgtccccgg atgttggtga tggagttagc
     6661 ctccaagggt tccttggatc gcctgcttca gcaggacaaa gccagcctca ctagaaccct
     6721 acagcacagg atcgcactcc acgtagctga tggtttgaga tacctccact cagccatgat
     6781 tatataccga gacctgaaac cccacaatgt gctgcttttc acactgtatc ccaatgctgc
     6841 catcatcgca aagattgctg actacggcat tgctcagtac tgctgtagaa tggggataaa
     6901 aacatcagag ggcacaccag ggtttcgtgc acctgaagtt gccagaggaa atgtcattta
     6961 taaccaacag gctgatgttt attcatttgg tttactactc tatgacattt tgacaactgg
     7021 aggtagaata gtagagggtt tgaagtttcc aaatgagttt gatgaattag aaatacaagg
     7081 aaaattacct gatccagtta aagaatatgg ttgtgcccca tggcctatgg ttgagaaatt
     7141 aattaaacag tgtttgaaag aaaatcctca agaaaggcct acttctgccc aggtctttga
     7201 cattttgaat tcagctgaat tagtctgtct gacgagacgc attttattac ctaaaaacgt
     7261 aattgttgaa tgcatggttg ctacacatca caacagcagg aatgcaagca tttggctggg
     7321 ctgtgggcac accgacagag gacagctctc atttcttgac ttaaatactg aaggatacac
     7381 ttctgaggaa gttgctgata gtagaatatt gtgcttagcc ttggtgcatc ttcctgttga
     7441 aaaggaaagc tggattgtgt ctgggacaca gtctggtact ctcctggtca tcaataccga
     7501 agatgggaaa aagagacata ccctagaaaa gatgactgat tctgtcactt gtttgtattg
     7561 caattccttt tccaagcaaa gcaaacaaaa aaattttctt ttggttggaa ccgctgatgg
     7621 caagttagca atttttgaag ataagactgt taagcttaaa ggagctgctc ctttgaagat
     7681 actaaatata ggaaatgtca gtactccatt gatgtgtttg agtgaatcca caaattcaac
     7741 ggaaagaaat gtaatgtggg gaggatgtgg cacaaagatt ttctcctttt ctaatgattt
     7801 caccattcag aaactcattg agacaagaac aagccaactg ttttcttatg cagctttcag
     7861 tgattccaac atcataacag tggtggtaga cactgctctc tatattgcta agcaaaatag
     7921 ccctgttgtg gaagtgtggg ataagaaaac tgaaaaactc tgtggactaa tagactgcgt
     7981 gcacttttta agggaggtaa tggtaaaaga aaacaaggaa tcaaaacaca aaatgtctta
     8041 ttctgggaga gtgaaaaccc tctgccttca gaagaacact gctctttgga taggaactgg
     8101 aggaggccat attttactcc tggatctttc aactcgtcga cttatacgtg taatttacaa
     8161 cttttgtaat tcggtcagag tcatgatgac agcacagcta ggaagcctta aaaatgtcat
     8221 gctggtattg ggctacaacc ggaaaaatac tgaaggtaca caaaagcaga aagagataca
     8281 atcttgcttg accgtttggg acatcaatct tccacatgaa gtgcaaaatt tagaaaaaca
     8341 cattgaagtg agaaaagaat tagctgaaaa aatgagacga acatctgttg agtaagagag
     8401 aagggcgaat tcgacccagc tttcttgtac aaagtggtga tcggtacctt aattaattaa
     8461 ggtaccaggt aagtgtaccc aattcgccct atagtgagtc gtattacaat tcactcgatc
     8521 gcccttccca acagttgcgc agcctgaatg gcgaatggag atccaatttt taagtgtata
     8581 atgtgttaaa ctactgattc taattgtttg tgtattttag attcacagtc ccaaggctca
     8641 tttcaggccc ctcagtcctc acagtctgtt catgatcata atcagccata ccacatttgt
     8701 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     8761 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     8821 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8881 caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg
     8941 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     9001 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     9061 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     9121 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     9181 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     9241 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     9301 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     9361 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     9421 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     9481 gaaaaaggaa gaatcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     9541 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     9601 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     9661 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     9721 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     9781 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     9841 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     9901 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     9961 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
    10021 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
    10081 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
    10141 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
    10201 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
    10261 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
    10321 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagaa catcaggggc
    10381 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
    10441 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
    10501 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
    10561 cccgtgatat tgctgaagaa cttggcggcg aatgggctga ccgcttcctc gtgctttacg
    10621 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
    10681 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
    10741 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
    10801 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
    10861 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
    10921 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
    10981 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
    11041 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
    11101 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
    11161 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    11221 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    11281 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    11341 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    11401 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
    11461 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    11521 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    11581 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    11641 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    11701 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    11761 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    11821 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    11881 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    11941 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgc
//