| 质粒名称: | pAAV-Syn-NES-SomaFRCaMPi |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001086 |
| 产品用途: | Syn promoted nuclear excluded plus soma-targeted version of red calcium indicator, FRCaMPi for in vivo calcium imaging |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 哺乳动物表达 |
| 启动子: |
Syn
|
| 拷贝数: | 中拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | NES-SomaFRCaMPi |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at single-cell resolution
Zhou S., Zhu Q., Eom M., Fang S., Subach O.M., Ran C., Alvarado J.S., Sunkavalli P.S., Dong Y., Wang Y., Hu J., Zhang H., Wang Z., Sun X., Yang T., Mu Y., Yoon Y.G., Guo Z.V., Subach F.V., Piatkevich K.D.
PLOS Biol. 23, e3003048 (2025)
|
pAAV-Syn-NES-SomaFRCaMPi
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-Syn-NES-SomaFRCaMPi载体质粒基本信息
pAAV-Syn-NES-SomaFRCaMPi质粒图谱载体图谱和pAAV-Syn-NES-SomaFRCaMPi载体序列质粒序列多克隆位点信息
pAAV-Syn-NES-SomaFRCaMPi质粒载体简介
pAAV-Syn-NES-SomaFRCaMPi质粒序列载体序列
LOCUS pAAV-Syn-NES-SomaFRCaMPi 6529 bp DNA circular SYN 14-SEP-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6529)
AUTHORS Kiryl Piatkevich
TITLE Direct Submission
JOURNAL Exported Aug 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6529
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 311..800
/label=Syn
promoter 349..796
/label=hSyn promoter
/note="human synapsin I promoter; confers neuron-specific
expression (Kugler et al., 2003)"
primer_bind 734..755
/label=Syn-F
primer_bind 788..843
/label=0826-(Syn)FRCaMPi-F
misc_feature 812..817
/label=Kozak sequence
CDS 818..2902
/codon_start=1
/label=NES-SomaFRCaMPi
/translation="MLQLPPLERLTLSRSMVSKGEEDNMAIIKEFMRFKVHMEGSVNGH
EFEIEGEGEGRPYEAFQTARLKVTKGGPLPFAWDILSPQFMYGSKAYIKHPADIPDYFK
LSFPEGFRWERVMSFEDGGIIHVNQDSSLQDGVFIYKVKLLGTNFPPDGPVMQKKTMGW
EASYEMTTRNLTDEQIAEFREAFSLLDRDQDGNITSNELGVVMRSLGLSPTAAELQDMI
NEVDADGNGTIDFTEFLTMMARKMKDTDDEEEVREAFKVFDKDGSGYITVEELTHVLTS
LGERLSREEVADVIREADSDGDGVINYEEFSRVISSKGGSGGSGSGSMRKPFRGAGNAV
RAFSTWKKLVPRVSEWMYPEDGALKSEIRKGLRLKDGGHYAAEVKTTYKAKKPVQLPGA
YIVDIELDILSHNEDYTIVEQCERAVGRHPTGGTDALYKGGSGGSGGTGGSGGSGGTGG
SGGSGGTSGRTQISSSSFEFSSKVSRDTLYEAVREVLHGNQRKRRKFLETVELQISLKN
YDPQKDKRFSGTVRLKSTPRPKFSVCVLGDQQHCDEAKAVDIPHMDIEALKKLNKNKKL
VKKLAKKYDAFLASESLIKQIPRILGPGLNKAGKFPSLLTHNENMVAKVDEVKSTIKFQ
MKKVLCLAVAVGHVKMTDDELVYNIHLAVNFLVSLLKKNWQNVRALYIKSTMGKPQRLY
"
misc_feature 2927..3515
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(3398..3409)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3533..3740
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 3792..3932
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4007..4462
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4744..4848
/gene="bla"
/label=AmpR promoter
CDS 4849..5709
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5880..6468
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgttgcaaa gatggataaa gttttaaaca gagaggaatc
181 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
241 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcagca
301 aattaattaa tggttaatta atctagactg cagagggccc tgcgtatgag tgcaagtggg
361 ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc ccgacccact
421 ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga gggggagggg
481 aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga cagtgccttc
541 gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc tgacgtcact
601 cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg ccgccgccgg
661 cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg accatctgcg
721 ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag gagtcgtgtc
781 gtgcctgaga gcgcagtcga gaggatccta agccaccatg cttcaacttc ctcctcttga
841 acgtcttact ctttcgagat ctatggtgag caagggcgag gaggataaca tggccatcat
901 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
961 gatcgagggc gagggcgagg gccgccccta cgaggccttc cagaccgcta ggctgaaggt
1021 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
1081 ctccaaggcc tacattaagc acccagccga catccccgac tacttcaagc tgtccttccc
1141 cgagggcttc aggtgggagc gcgtgatgag cttcgaggac ggcggcatta ttcacgttaa
1201 tcaggactcc tccctgcagg acggcgtatt catctacaag gtgaagctgc tcggcaccaa
1261 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cttcctacga
1321 gatgactacc cgtaacctta cagatgagca gattgcggag ttccgtgagg ccttttcgct
1381 gcttgatcgt gatcaggatg gaaatatcac gtccaatgaa ttgggtgtgg ttatgaggtc
1441 gttaggtcta tcgcctactg ccgccgaatt acaagatatg attaatgagg tcgatgccga
1501 tggtaatggc acaattgatt ttaccgaatt tttgacaatg atggcccgga aaatgaagga
1561 taccgacgac gaagaggaag ttcgcgaagc ctttaaagtc ttcgataaag atggaagtgg
1621 atacattaca gtcgaggagc tgactcatgt tcttacaagt ctcggtgaac gtttgtctcg
1681 agaagaagta gccgatgtga tacgtgaagc cgactccgat ggcgatggtg taatcaacta
1741 cgaagaattt tctcgtgtca tttcttccaa gggcggttca ggaggcagcg ggtcaggttc
1801 aatgaggaaa ccgttccgtg gcgcgggcaa cgctgtgcgt gctttcagca cttggaaaaa
1861 gctagtgccg cgggtttccg agtggatgta ccccgaggac ggcgccctga agagcgagat
1921 caggaagggg ctgaggctga aggacggcgg ccactacgcc gccgaggtca agaccaccta
1981 caaggccaag aagcccgtgc agctgcccgg cgcctacatc gtcgacatcg agttggacat
2041 cttgtcccac aacgaggact acaccatcgt ggaacagtgc gaacgcgccg tgggccgcca
2101 ccccaccggt ggcacggacg cgctgtacaa gggcggatct ggcggtagcg gagggacagg
2161 tggctctgga gggagcggag gcactggcgg atctggtggg agcggaggta cctcaggccg
2221 gactcagatc tcaagctcaa gcttcgagtt cagcagcaaa gtctcacgcg acaccctgta
2281 cgaggcggtg cgggaagtcc tgcacgggaa ccagcgcaag cgccgcaagt ttctggagac
2341 ggtggagctg cagatcagcc tgaagaacta cgaccctcag aaggacaaac gtttctcggg
2401 caccgtcagg ctcaagtcca ccccacgccc caagttctcg gtgtgcgttc tgggggacca
2461 gcagcactgt gatgaagcca aggccgtgga tatcccccac atggacatcg aggcgctcaa
2521 gaaacttaac aaaaacaaga agttggtcaa gaagctggct aagaagtacg atgccttttt
2581 ggcctctgag tctctgatta agcagatccc acgtatcctg ggcccaggcc taaacaaggc
2641 tggcaagttc ccctccctgc tgacacacaa tgaaaacatg gtggccaaag tggatgaggt
2701 gaaatcgaca atcaagttcc agatgaagaa ggtgctgtgt ttggccgtcg ctgttggcca
2761 cgtgaagatg accgatgatg agctagtcta caacattcat ctggctgtca atttcttggt
2821 gtccttgctt aagaaaaact ggcaaaacgt gcgggctctg tacatcaaga gcaccatggg
2881 caagccccag cgtctgtatt aggaattccg tacaaagctt atcgataatc aacctctgga
2941 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
3001 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
3061 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
3121 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
3181 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
3241 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
3301 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct atgttgccac
3361 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
3421 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
3481 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccggctcg agctgtgcct
3541 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
3601 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
3661 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
3721 aatagcaggc atgctgggga tgcggtctcg agctgatttt gtaggtaacc acgtgcggac
3781 cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
3841 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc
3901 agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac
3961 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
4021 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
4081 gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
4141 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
4201 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
4261 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
4321 actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg
4381 atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
4441 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
4501 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
4561 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
4621 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
4681 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
4741 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
4801 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
4861 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
4921 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
4981 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
5041 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
5101 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
5161 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
5221 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
5281 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
5341 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
5401 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
5461 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
5521 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
5581 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
5641 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
5701 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
5761 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
5821 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
5881 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
5941 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
6001 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
6061 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
6121 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
6181 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
6241 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
6301 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
6361 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
6421 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
6481 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
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