pAAVLINK-EF1a-FBN1-A

LOCUS       pAAVLINK-EF1a-FBN1-A                5954 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 8, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        158..700
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    505..510
                     /label=SDS
     CDS             713..3094
                     /codon_start=1
                     /label=FBN1
                     /translation="MRRGRLLEIALGFTVLLASYTSHGADANLEAGNVKETRASRAKRR
                     GGGGHDALKGPNVCGSRYNAYCCPGWKTLPGGNQCIVPICRHSCGDGFCSRPNMCTCPS
                     GQIAPSCGSRSIQHCNIRCMNGGSCSDDHCLCQKGYIGTHCGQPVCESGCLNGGRCVAP
                     NRCACTYGFTGPQCERDYRTGPCFTVISNQMCQGQLSGIVCTKTLCCATVGRAWGHPCE
                     MCPAQPHPCRRGFIPNIRTGACQDVDECQAIPGLCQGGNCINTVGSFECKCPAGHKLNE
                     VSQKCEDIDECSTIPGICEGGECTNTVSSYFCKCPPGFYTSPDGTRCIDVRPGYCYTAL
                     TNGRCSNQLPQSITKMQCCCDAGRCWSPGVTVAPEMCPIRATEDFNKLCSVPMVIPGRP
                     EYPPPPLGPIPPVLPVPPGFPPGPQIPVPRPPVEYLYPSREPPRVLPVNVTDYCQLVRY
                     LCQNGRCIPTPGSYRCECNKGFQLDLRGECIDVDECEKNPCAGGECINNQGSYTCQCRA
                     GYQSTLTRTECRDIDECLQNGRICNNGRCINTDGSFHCVCNAGFHVTRDGKNCEDMDEC
                     SIRNMCLNGMCINEDGSFKCICKPGFQLASDGRYCKDINECETPGICMNGRCVNTDGSY
                     RCECFPGLAVGLDGRVCVDTHMRSTCYGGYKRGQCIKPLFGAVTKSECCCASTEYAFGE
                     PCQPCPAQNSAEYQALCSSGPGMTSAGSDINECALDPDICPNGICENLRGTYKCICNSG
                     YEVDSTGKNCVDINECVLNSLLCDNGQCRNTPGSFVCTCPK"
     misc_feature    1958..1963
                     /label=SDS
     misc_feature    1970..1975
                     /label=SDS
     intron          3095..3135
                     /label=Splicing Donor
     misc_feature    3139..3172
                     /label=loxP-JT1571
     repeat_region   3217..3357
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3217..3346
                     /label=AAV2 ITR
     rep_origin      3432..3887
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4169..4273
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4274..5134
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5305..5893
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgcgtcg
      721 agggcgtctg ctggagatcg ccctgggatt taccgtgctt ttagcgtcct acacgagcca
      781 tggggcggac gccaatttgg aggctgggaa cgtgaaggaa accagagcca gtcgggccaa
      841 gagaagaggc ggtggaggac acgacgcgct taaaggaccc aatgtctgtg gatcacgtta
      901 taatgcttac tgttgccctg gatggaaaac cttacctggc ggaaatcagt gtattgtccc
      961 catttgccgg cattcctgtg gggatggatt ttgttcgagg ccaaatatgt gcacttgccc
     1021 atctggtcag atagctcctt cctgtggctc cagatccata caacactgca atattcgctg
     1081 tatgaatgga ggtagctgca gtgacgatca ctgtctatgc cagaaaggat acatagggac
     1141 tcactgtgga caacctgttt gtgaaagtgg ctgtctcaat ggaggaaggt gtgtggcccc
     1201 aaatcgatgt gcatgcactt acggatttac tggaccccag tgtgaaagag attacaggac
     1261 aggcccatgt tttactgtga tcagcaacca gatgtgccag ggacaactca gcgggattgt
     1321 ctgcacaaaa acgctctgct gtgccacagt cggccgagcc tggggccacc cctgtgagat
     1381 gtgtcctgcc cagcctcacc cctgccgccg tggcttcatt ccaaatatcc gcacgggagc
     1441 ttgtcaagat gtggatgaat gccaggccat ccccgggctc tgtcagggag gaaattgcat
     1501 taatactgtt gggtcttttg agtgcaaatg ccctgctgga cacaaactta atgaagtgtc
     1561 acaaaaatgt gaagatattg atgaatgcag caccattcct ggaatctgtg aagggggtga
     1621 atgtacaaac acagtcagca gttacttttg caaatgtccc cctggttttt acacctctcc
     1681 agatggtacc agatgcatag atgttcgccc aggatactgt tacacagctc tgacaaacgg
     1741 gcgctgctct aaccagctgc cacagtccat aaccaaaatg cagtgctgct gtgatgccgg
     1801 ccgatgctgg tctccagggg tcactgtcgc ccctgagatg tgtcccatca gagcaaccga
     1861 ggatttcaac aagctgtgct ctgttcctat ggtaattcct gggagaccag aatatcctcc
     1921 cccacccctt ggccccattc ctccagttct ccctgttcct cctggctttc ctcctggacc
     1981 tcaaattccg gtccctcgac caccagtgga atatctgtat ccatctcggg agccaccaag
     2041 ggtgctgcca gtaaacgtta ctgattactg ccagttggtc cgctatctct gtcaaaatgg
     2101 acgctgcatt ccaactcctg ggagttaccg gtgtgagtgc aacaaagggt tccagctgga
     2161 cctccgtggg gagtgtattg atgttgatga atgtgagaaa aacccctgtg ctggtggtga
     2221 gtgtattaac aaccagggtt cgtacacctg tcagtgccga gctggatatc agagcacact
     2281 cacgcggaca gaatgccgag acattgatga gtgtttacag aatggccgga tctgcaataa
     2341 tggacgctgc atcaacacag atggcagttt tcattgcgtg tgtaatgcgg gctttcatgt
     2401 tacacgagat gggaagaact gtgaagatat ggatgaatgc agcataagga acatgtgcct
     2461 taatggaatg tgtatcaatg aagatggcag ttttaaatgt atttgcaaac ctggattcca
     2521 gctggcatca gatggacgtt attgcaaaga cattaacgag tgtgaaaccc ctgggatctg
     2581 catgaatggg cgttgcgtca acactgatgg ctcctacaga tgtgaatgct tccctggact
     2641 ggctgtgggt ctggatggcc gtgtgtgtgt tgacacacac atgcggagca catgctatgg
     2701 tggatacaag agaggccagt gtatcaaacc tttgtttggt gctgtcacta aatctgaatg
     2761 ctgttgcgcc agcactgagt atgcatttgg ggaaccttgc cagccgtgtc ctgcacagaa
     2821 ttcagcggaa tatcaggcac tctgcagcag tgggccagga atgacgtcag caggcagtga
     2881 tataaatgaa tgtgcactag atcctgatat ttgcccaaat ggaatctgtg aaaaccttcg
     2941 tgggacctat aaatgtatat gcaattcagg atatgaagtg gattcaactg ggaaaaactg
     3001 cgttgatatt aatgaatgtg tactgaacag tctcctttgt gacaatggac aatgtagaaa
     3061 tactcctgga agttttgtct gtacctgccc caaggtaagt atcaaggtta caagacaggt
     3121 ttaaggagac caataagcta ttattcgtat agcatacatt atacgaagtt attaagttat
     3181 ggagaccggt gtggcacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
     3241 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     3301 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
     3361 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
     3421 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     3481 cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc
     3541 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     3601 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     3661 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     3721 ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc
     3781 ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta
     3841 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
     3901 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     3961 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     4021 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     4081 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     4141 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4201 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4261 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4321 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     4381 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     4441 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     4501 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     4561 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     4621 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     4681 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     4741 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     4801 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     4861 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     4921 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     4981 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     5041 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     5101 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5161 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5221 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5281 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5341 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5401 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     5461 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5521 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5581 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5641 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5701 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5761 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5821 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5881 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5941 ttttgctcac atgt
//