| 质粒名称: | pAAVLINK-EF1a-5'-ROBO1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001680 |
| 产品用途: | 5' AAVLINK plasmid for ROBO1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ROBO1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-5'-ROBO1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-5'-ROBO1载体质粒基本信息
pAAVLINK-EF1a-5'-ROBO1质粒图谱载体图谱和pAAVLINK-EF1a-5'-ROBO1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-5'-ROBO1质粒载体简介
pAAVLINK-EF1a-5'-ROBO1质粒序列载体序列
LOCUS pAAVLINK-EF1a-5'-ROBO1 6254 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6254)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 2, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6254
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 713..3416
/codon_start=1
/label=ROBO1
/translation="MKWKHVPFLVMISLLSLSPNHLFLAQLIPDPEDVERGNDHGTPIP
TSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKG
GERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASL
EVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRG
GKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEF
KCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAE
ASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPP
QSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIR
QGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKL
GDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNT
VTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVR
AANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVH
WTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARP
FFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQE
YKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQL
DAHGNPVSPEDQVSLAQQISDVVKQPAFIA"
misc_feature 1375..1380
/label=SDS
intron 3417..3457
/label=Splicing Donor
misc_feature 3461..3494
/label=lox2272-JT1571
repeat_region 3539..3657
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3539..3646
/label=AAV2 ITR
rep_origin 3732..4187
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4469..4573
/gene="bla"
/label=AmpR promoter
CDS 4574..5434
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5605..6193
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgaaatg
721 gaaacatgtt ccttttttgg tcatgatatc actcctcagc ttatccccaa atcacctgtt
781 tctggcccag cttattccag accctgaaga tgtagagagg gggaacgacc acgggacgcc
841 aatccccacc tctgataacg atgacaattc gctgggctat acaggctccc gtcttcgtca
901 ggaagatttt ccacctcgca ttgttgaaca cccttcagac ctgattgtct caaaaggaga
961 acctgcaact ttgaactgca aagctgaagg ccgccccaca cccactattg aatggtacaa
1021 agggggagag agagtggaga cagacaaaga tgaccctcgc tcacaccgaa tgttgctgcc
1081 gagtggatct ttatttttct tacgtatagt acatggacgg aaaagtagac ctgatgaagg
1141 agtctatgtc tgtgtagcaa ggaattacct tggagaggct gtgagccaca atgcatcgct
1201 ggaagtagcc atacttcggg atgacttcag acaaaaccct tcggatgtca tggttgcagt
1261 aggagagcct gcagtaatgg aatgccaacc tccacgaggc catcctgagc ccaccatttc
1321 atggaagaaa gatggctctc cactggatga taaagatgaa agaataacta tacgaggagg
1381 aaagctcatg atcacttaca cccgtaaaag tgacgctggc aaatatgttt gtgttggtac
1441 caatatggtt ggggaacgtg agagtgaagt agccgagctg actgtcttag agagaccatc
1501 atttgtgaag agacccagta acttggcagt aactgtggat gacagtgcag aatttaaatg
1561 tgaggcccga ggtgaccctg tacctacagt acgatggagg aaagatgatg gagagctgcc
1621 caaatccaga tatgaaatcc gagatgatca taccttgaaa attaggaagg tgacagctgg
1681 tgacatgggt tcatacactt gtgttgcaga aaatatggtg ggcaaagctg aagcatctgc
1741 tactctgact gttcaagaac ctccacattt tgttgtgaaa ccccgtgacc aggttgttgc
1801 tttgggacgg actgtaactt ttcagtgtga agcaaccgga aatcctcaac cagctatttt
1861 ctggaggaga gaagggagtc agaatctact tttctcatat caaccaccac agtcatccag
1921 ccgattttca gtctcccaga ctggcgacct cacaattact aatgtccagc gatctgatgt
1981 tggttattac atctgccaga ctttaaatgt tgctggaagc atcatcacaa aggcatattt
2041 ggaagttaca gatgtgattg cagatcggcc tcccccagtt attcgacaag gtcctgtgaa
2101 tcagactgta gccgtggatg gcactttcgt cctcagctgt gtggccacag gcagtccagt
2161 gcccaccatt ctgtggagaa aggatggagt cctcgtttca acccaagact ctcgaatcaa
2221 acagttggag aatggagtac tgcagatccg atatgctaag ctgggtgata ctggtcggta
2281 cacctgcatt gcatcaaccc ccagtggtga agcaacatgg agtgcttaca ttgaagttca
2341 agaatttgga gttccagttc agcctccaag acctactgac ccaaatttaa tccctagtgc
2401 cccatcaaaa cctgaagtga cagatgtcag cagaaataca gtcacattat cgtggcaacc
2461 aaatttgaat tcaggagcaa ctccaacatc ttatattata gaagccttca gccatgcatc
2521 tggtagcagc tggcagaccg tagcagagaa tgtgaaaaca gaaacatctg ccattaaagg
2581 actcaaacct aatgcaattt accttttcct tgtgagggca gctaatgcat atggaattag
2641 tgatccaagc caaatatcag atccagtgaa aacacaagat gtcctaccaa caagtcaggg
2701 ggtggaccac aagcaggtcc agagagagct gggaaatgct gttctgcacc tccacaaccc
2761 caccgtcctt tcttcctctt ccatcgaagt gcactggaca gtagatcaac agtctcagta
2821 tatacaagga tataaaattc tctatcggcc atctggagcc aaccacggag aatcagactg
2881 gttagttttt gaagtgagga cgccagccaa aaacagtgtg gtaatccctg atctcagaaa
2941 gggagtcaac tatgaaatta aggctcgccc tttttttaat gaatttcaag gagcagatag
3001 tgaaatcaag tttgccaaaa ccctggaaga agcacccagt gccccacccc aaggtgtaac
3061 tgtatccaag aatgatggaa acggaactgc aattctagtt agttggcagc cacctccaga
3121 agacactcaa aatggaatgg tccaagagta taaggtttgg tgtctgggca atgaaactcg
3181 ataccacatc aacaaaacag tggatggttc caccttttcc gtggtcattc cctttcttgt
3241 tcctggaatc cgatacagtg tggaagtggc agccagcact ggggctgggt ctggggtaaa
3301 gagtgagcct cagttcatcc agctggatgc ccatggaaac cctgtgtcac ctgaggacca
3361 agtcagcctc gctcagcaga tttcagatgt ggtgaagcag ccggccttca tagcaggtaa
3421 gtatcaaggt tacaagacag gtttaaggag accaataagc tattattcgt ataggatact
3481 ttatacgaag ttattaagtt atggagaccg gtgtggcacg tgcggaccga gcggccgcag
3541 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
3601 gggcgaccaa aggtcgcccg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
3661 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
3721 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
3781 cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc
3841 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
3901 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
3961 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
4021 ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc
4081 ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta
4141 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
4201 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
4261 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
4321 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
4381 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
4441 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
4501 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
4561 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
4621 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
4681 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
4741 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
4801 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
4861 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
4921 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
4981 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
5041 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
5101 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
5161 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
5221 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
5281 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
5341 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
5401 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
5461 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
5521 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
5581 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
5641 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
5701 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
5761 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
5821 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
5881 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
5941 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
6001 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
6061 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
6121 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
6181 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
6241 ttttgctcac atgt
//
pAAVLINK-EF1a-5'-ROBO1载体图谱质粒图谱pdf版和pAAVLINK-EF1a-5'-ROBO1载体序列质粒序列等相关资料下载
pAAVLINK-EF1a-5'-ROBO1质粒载体应用举例
- 上一篇：pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ABCC6-FLAG
- 下一篇：没有了
