| 出品公司: | Clontech |
|---|---|
| 载体名称: | pmRaspberry |
| 质粒类型: | 荧光蛋白基因质粒 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 3314 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | M13 rev |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
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pmRaspberry载体质粒基本信息
pmRaspberry质粒图谱载体图谱和pmRaspberry载体序列质粒序列多克隆位点信息
pmRaspberry质粒载体简介
pmRaspberry质粒序列载体序列
LOCUS pmRaspberry 3314 bp ds-DNA circular SYN 04-1-2013
DEFINITION Vector for expressing mRaspberry in bacteria.
ACCESSION .
VERSION .
KEYWORDS pmRaspberry
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3314)
AUTHORS Clontech
TITLE Direct Submission
JOURNAL Exported 2017/2/28 from SnapGene 2.3.2
http://www.snapgene.com
COMMENT Translation from the lacZ start codon produces a fusion protein in
E. coli.
FEATURES Location/Qualifiers
source 1..3314
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
promoter 143..173
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 205..221
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
CDS 217..219
/codon_start=1
/product="lacZ start codon"
/note="ATG"
/translation="M"
CDS 289..969
/codon_start=1
/product="far-red monomeric derivative of DsRed fluorescent
protein"
/note="mRaspberry"
/note="mammalian codon-optimized"
/translation="MVSKGEEVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFAWDILSPQCMYGSKGYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
VVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
MRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
STGA"
promoter 1409..1513
/gene="bla"
/note="AmpR promoter"
CDS 1514..2374
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2545..3133
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
241 catgcctgca ggtcgactct agaggatccc cgggtaccgg tcgccaccat ggtgagcaag
301 ggcgaggagg tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg ctccgtgaac
361 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
421 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
481 cagtgcatgt acggctccaa gggctacgtg aagcaccccg ccgacatccc cgactacttg
541 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
601 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
661 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
721 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatgaagatg
781 aggctgaagc tgaaggacgg cggccactac gacgccgagg tcaagaccac ctacatggcc
841 aagaagcccg tgcagctgcc cggcgcctac aagaccgaca tcaagctgga catcacctcc
901 cacaacgagg actacaccat cgtggaacag tacgagcgcg ccgagggccg ccactccacc
961 ggcgcctagc ggccgcgact ctagaattcc aactgagcgc cggtcgctac cattaccaac
1021 ttgtctggtg tcaaaaataa taggcctact agtcggccgt acgggccctt tcgtctcgcg
1081 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
1141 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
1201 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
1261 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcggccttaa
1321 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
1381 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
1441 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
1501 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
1561 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
1621 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
1681 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
1741 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
1801 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
1861 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
1921 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
1981 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
2041 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
2101 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
2161 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
2221 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
2281 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
2341 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
2401 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
2461 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
2521 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
2581 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
2641 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
2701 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
2761 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
2821 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
2881 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
2941 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
3001 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
3061 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
3121 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
3181 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
3241 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
3301 cgaggaagcg gaag
//
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