| 质粒名称: | pET30a-HS-Tn5 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 127916 |
| 存储实验室: | Silin Zhong |
|
载体骨架基本信息 |
|
| 载体名称: | pET30a |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 8303 bp |
| 载体类型: | 细菌表达 |
| 载体抗性: | Kanamycin, 50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | HS-Tn5 |
| 基因突变: | E54K, L372P |
| 启动子: | T7 |
| 标签/融合蛋白: |
E coli elongation factor Ts (N terminal on insert)
Mxe intein - Chitin-binding domain (C terminal on insert)
|
| 克隆方法: | Gibson Cloning |
| 5’测序引物 | T7 |
| 3’测序引物 | T7ter |
|
文献引用方法 |
|
| 材料和方法部分: | pET30a-HS-Tn5 was a gift from Silin Zhong (Addgene plasmid # 127916 ; http://n2t.net/addgene:127916 ; RRID:Addgene_127916) |
| 参考文献部分: | Reconstructing the maize leaf regulatory network using ChIP-seq data of 104 transcription factors. Tu X, Mejia-Guerra MK, Valdes Franco JA, Tzeng D, Chu PY, Shen W, Wei Y, Dai X, Li P, Buckler ES, Zhong S. Nat Commun. 2020 Oct 9;11(1):5089. doi: 10.1038/s41467-020-18832-8. 10.1038/s41467-020-18832-8 PubMed 33037196 |
买家导航
pET30a-HS-Tn5载体质粒基本信息
pET30a-HS-Tn5质粒图谱载体图谱和pET30a-HS-Tn5载体序列质粒序列多克隆位点信息
pET30a-HS-Tn5质粒载体简介
pET30a-HS-Tn5质粒序列载体序列
LOCUS pET30a-HS-Tn5 8303 bp DNA circular SYN 14-APR-2025
DEFINITION Prokaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina
library preperation. Developed for the large scale ChIP-Seq and
ATAC-Seq experiments for the fruitENCODE and Maize Cistrome
projects..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8303)
AUTHORS Tu X, Mejia-Guerra MK, Valdes Franco JA, Tzeng D, Chu PY, Shen W,
Wei Y, Dai X, Li P, Buckler ES, Zhong S
TITLE Reconstructing the maize leaf regulatory network using ChIP-seq data
of 104 transcription factors.
JOURNAL Nat Commun. 2020 Oct 9;11(1):5089. doi: 10.1038/s41467-020-18832-8.
PUBMED 33037196
REFERENCE 2 (bases 1 to 8303)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat
Commun."; date: "2020-10-9"; volume: "11(1):5089. doi"; pages: "
10.1038/s41467-020-18832-8"
COMMENT Sequence Label: pET30a-HS-Tn5
FEATURES Location/Qualifiers
source 1..8303
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 1..20
/label=T7
/note="T7 promoter, forward primer"
promoter 1..19
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 20..44
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 59..81
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 938..2362
/codon_start=1
/product="transposase from the bacterial Tn5 transposon
(Reznikoff, 1993)"
/label=Tn5 transposase
/translation="ITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKSI
TISSEGSKAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYRH
QVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESGK
WLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGLY
LYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNAV
LAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAERQ
RMEEPDNLERMVSILSFVAVRLLQLRESFTPPQALRAQGLLKEAEHVESQSAETVLTPD
ECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQSK
LDGFLAAKDLMAQGIKI"
CDS 2363..2956
/codon_start=1
/gene="Mycobacterium xenopi gyrA"
/product="modified GyrA intein (Southworth et al., 1999)"
/label=Mxe GyrA intein
/note="N-terminal cleavage of this intein can be induced by
thiols."
/translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
CDS 2987..3142
/codon_start=1
/gene="Bacillus circulans chiA"
/product="chitin binding domain from chitinase A1 (Watanabe
et al., 1994)"
/label=CBD
/translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
PALWQLQ"
CDS 3164..3181
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(3234..3252)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 3248..3295
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 3332..3787
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3419..3438)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3629..3650
/label=F1ori-F
/note="F1 origin, forward primer"
CDS complement(3879..4694)
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
CDS complement(3879..4694)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind 4602..4621
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 4686..4705
/label=pENTR-R
/note="pENTR vectors, reverse primer"
rep_origin 4816..5404
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5305..5324
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 5558..5575
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 5590..5729
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(5655..5674)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 5815..5837
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(5831..6022)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
CDS complement(6831..7913)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind 7875..7894
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(7914..7991)
/gene="lacI"
/label=lacI promoter
primer_bind 8197..8216
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
ORIGIN
1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
61 tgtttaactt taagaaggag atatacatat ggctgaaatt accgcatccc tggtaaaaga
121 gctgcgtgag cgtactggcg caggcatgat ggattgcaaa aaagcactga ctgaagctaa
181 cggcgacatc gagctggcaa tcgaaaacat gcgtaagtcc ggtgctatta aagcagcgaa
241 aaaagcaggc aacgttgctg ctgacggcgt gatcaaaacc aaaatcgacg gcaactacgg
301 catcattctg gaagttaact gccagactga cttcgttgca aaagacgctg gtttccaggc
361 gttcgcagac aaagttctgg acgcagctgt tgctggcaaa atcactgacg ttgaagttct
421 gaaagcacag ttcgaagaag aacgtgttgc gctggtagcg aaaattggtg aaaacatcaa
481 cattcgccgc gttgctgcgc tggaaggcga cgttctgggt tcttatcagc acggtgcgcg
541 tatcggcgtt ctggttgctg ctaaaggcgc tgacgaagag ctggttaaac acatcgctat
601 gcacgttgct gcaagcaagc cagaattcat caaaccggaa gacgtatccg ctgaagtggt
661 agaaaaagaa taccaggtac agctggatat cgcgatgcag tctggtaagc cgaaagaaat
721 cgcagagaaa atggttgaag gccgcatgaa gaaattcacc ggcgaagttt ctctgaccgg
781 tcagccgttc gttatggaac caagcaaaac tgttggtcag ctgctgaaag agcataacgc
841 tgaagtgact ggcttcatcc gcttcgaagt gggtgaaggc atcgagaaag ttgagactga
901 ctttgcagca gaagttgctg cgatgtccaa gcagtctatt accagtgcac tgcatcgtgc
961 ggcggattgg gcgaaaagcg tgttttctag tgctgcgctg ggtgatccgc gtcgtaccgc
1021 gcgtctggtg aatgttgcgg cgcaactggc caaatatagc ggcaaaagca ttaccattag
1081 cagcgaaggc agcaaagcca tgcaggaagg cgcgtatcgt tttattcgta atccgaacgt
1141 gagcgcggaa gcgattcgta aagcgggtgc catgcagacc gtgaaactgg cccaggaatt
1201 tccggaactg ctggcaattg aagataccac ctctctgagc tatcgtcatc aggtggcgga
1261 agaactgggc aaactgggta gcattcagga taaaagccgt ggttggtggg tgcatagcgt
1321 gctgctgctg gaagcgacca cctttcgtac cgtgggcctg ctgcatcaag aatggtggat
1381 gcgtccggat gatccggcgg atgcggatga aaaagaaagc ggcaaatggc tggccgctgc
1441 tgcaacttcg cgtctgagaa tgggcagcat gatgagcaac gtgattgcgg tgtgcgatcg
1501 tgaagcggat attcatgcgt atctgcaaga taaactggcc cataacgaac gttttgtggt
1561 gcgtagcaaa catccgcgta aagatgtgga aagcggcctg tatctgtatg atcacctgaa
1621 aaaccagccg gaactgggcg gctatcagat tagcattccg cagaaaggcg tggtggataa
1681 acgtggcaaa cgtaaaaacc gtccggcgcg taaagcgagc ctgagcctgc gtagcggccg
1741 tattaccctg aaacagggca acattaccct gaacgcggtg ctggccgaag aaattaatcc
1801 gccgaaaggc gaaaccccgc tgaaatggct gctgctgacc agcgagccgg tggaaagtct
1861 ggcccaagcg ctgcgtgtga ttgatattta tacccatcgt tggcgcattg aagaatttca
1921 caaagcgtgg aaaacgggtg cgggtgcgga acgtcagcgt atggaagaac cggataacct
1981 ggaacgtatg gtgagcattc tgagctttgt ggcggtgcgt ctgctgcaac tgcgtgaatc
2041 ttttactccg ccgcaagcac tgcgtgcgca gggcctgctg aaagaagcgg aacacgttga
2101 aagccagagc gcggaaaccg tgctgacccc ggatgaatgc caactgctgg gctatctgga
2161 taaaggcaaa cgcaaacgca aagaaaaagc gggcagcctg caatgggcgt atatggcgat
2221 tgcgcgtctg ggcggcttta tggatagcaa acgtaccggc attgcgagct ggggtgcgct
2281 gtgggaaggt tgggaagcgc tgcaaagcaa actggatggc tttctggccg cgaaagacct
2341 gatggcgcag ggcattaaaa tctgcatcac gggagatgca ctagttgccc tacccgaggg
2401 cgagtcggta cgcatcgccg acatcgtgcc gggtgcgcgg cccaacagtg acaacgccat
2461 cgacctgaaa gtccttgacc ggcatggcaa tcccgtgctc gccgaccggc tgttccactc
2521 cggcgagcat ccggtgtaca cggtgcgtac ggtcgaaggt ctgcgtgtga cgggcaccgc
2581 gaaccacccg ttgttgtgtt tggtcgacgt cgccggggtg ccgaccctgc tgtggaagct
2641 gatcgacgaa atcaagccgg gcgattacgc ggtgattcaa cgcagcgcat tcagcgtcga
2701 ctgtgcaggt tttgcccgcg ggaaacccga atttgcgccc acaacctaca cagtcggcgt
2761 ccctggactg gtgcgtttct tggaagcaca ccaccgagac ccggacgccc aagctatcgc
2821 cgacgagctg accgacgggc ggttctacta cgcgaaagtc gccagtgtca ccgacgccgg
2881 cgtgcagccg gtgtatagcc ttcgtgtcga cacggcagac cacgcgttta tcacgaacgg
2941 gttcgtcagc cacgctactg gcctcaccgg tctgaactca ggcctcacga caaatcctgg
3001 tgtatccgct tggcaggtca acacagctta tactgcggga caattggtca catataacgg
3061 caagacgtat aaatgtttgc agccccacac ctccttggca ggatgggaac catccaacgt
3121 tcctgccttg tggcagcttc aaaagcttgc ggccgcactc gagcaccacc accaccacca
3181 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
3241 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
3301 aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
3361 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
3421 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
3481 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
3541 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
3601 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
3661 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
3721 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
3781 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
3841 aaatacattc aaatatgtat ccgctcatga attaattctt agaaaaactc atcgagcatc
3901 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
3961 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
4021 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
4081 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
4141 agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
4201 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
4261 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
4321 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
4381 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
4441 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
4501 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
4561 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
4621 ccatataaat cagcatccat gttggaattt aatcgcggcc tagagcaaga cgtttcccgt
4681 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
4741 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
4801 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
4861 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
4921 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
4981 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
5041 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
5101 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
5161 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
5221 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
5281 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
5341 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
5401 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
5461 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
5521 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
5581 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa
5641 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
5701 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
5761 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
5821 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa
5881 gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct
5941 cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg
6001 cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg
6061 taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg
6121 cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga
6181 gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg
6241 gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc
6301 gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg
6361 cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct
6421 aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc
6481 gcacccgtgg ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg
6541 cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca
6601 ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg
6661 ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag
6721 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc
6781 gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg
6841 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
6901 gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac
6961 agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt
7021 tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg
7081 tcttcggtat cgtcgtatcc cactaccgag atgtccgcac caacgcgcag cccggactcg
7141 gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga
7201 acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg
7261 ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc
7321 agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga
7381 cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata
7441 ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca
7501 gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg
7561 cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc
7621 atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt
7681 tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg
7741 cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc
7801 actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc
7861 tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc
7921 accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat
7981 tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc
8041 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat
8101 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
8161 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
8221 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga
8281 gatcgatctc gatcccgcga aat
//
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