pCMV-hA3D-BE3

LOCUS       pCMV-hA3D-BE3                9003 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses hA3D-BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-hA3D-BE3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9003)
  AUTHORS   Wang X, Li J, Wang Y, Yang V, Wei J, Wu J, Wang R, Huang X, Chen J, 
            Yang L
  TITLE     Efficient base editing in methylated regions with a human 
            APOBEC3A-Cas9 fusion.
  JOURNAL   Nat Biotechnol.,  2018
REFERENCE   2  (bases 1 to 9003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9003
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        159..538
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        539..742
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     693..713
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     784..803
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        784..802
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2022..6122
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6135..6383
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6396..6416
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6425..6442
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6465..6482)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6471..6695
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6766..6782)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6766..6782)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6779..6801)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6790..6806
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6814..6844)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6859..6880
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6997..7014)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7168..7756)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7248..7267)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7927..8787)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8550..8569
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8788..8892)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8971..8990)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
       61 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
      121 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
      181 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      241 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      301 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      361 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      421 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      481 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      541 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      601 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      661 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      721 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctaga gatccgcggc
      781 cgctaatacg actcactata gggagagccg ccaccatgaa tccacagatc agaaatccga
      841 tggagcggat gtatcgagac acattctacg acaactttga aaacgaaccc atcctctatg
      901 gtcggagcta cacttggctg tgctatgaag tgaaaataaa gaggggccgc tcaaatctcc
      961 tttgggacac aggggtcttt cgaggcccgg tactacccaa acgtcagtcg aatcacaggc
     1021 aggaggtgta tttccggttt gagaaccacg cagaaatgtg cttcttatct tggttctgtg
     1081 gcaaccgact gcctgctaac aggcgcttcc agatcacctg gtttgtatca tggaacccct
     1141 gcctgccctg tgtggtgaag gtgaccaaat tcttggctga gcaccccaat gtcaccctga
     1201 ccatctctgc cgcccgcctc tactactacc gggatagaga ttggcggtgg gtgctcctca
     1261 ggctgcataa ggcaggggcc cgtgtgaaga tcatggacta tgaagacttt gcatactgct
     1321 gggaaaactt tgtgtgcaat gaaggtcagc cattcatgcc ttggtacaaa ttcgatgaca
     1381 attatgcatc cctgcaccgc acgctaaagg agattctcag aaacccgatg gaggcaatgt
     1441 acccacacat attctacttc cactttaaaa acctactgaa agcctgtggt cggaacgaaa
     1501 gctggctgtg cttcaccatg gaagttacaa agcaccactc agctgtcttc cggaagaggg
     1561 gcgtcttccg aaaccaggtg gatcctgaga cccattgtca tgcagaaagg tgcttcctct
     1621 cttggttctg tgacgacata ctgtctccta acacaaacta cgaggtcacc tggtacacat
     1681 cttggagccc ttgcccagag tgtgcagggg aggtggccga gttcctggcc aggcacagca
     1741 acgtgaatct caccatcttc accgcccgcc tctgctactt ctgggataca gattaccagg
     1801 aggggctctg cagcctgagt caggaagggg cctccgtgaa gatcatgggc tacaaagatt
     1861 ttgtatcttg ttggaaaaac tttgtgtaca gtgatgatga gccattcaag ccttggaagg
     1921 gactacaaac caactttcga cttctgaaaa gaaggctacg ggagattctc cagagcggca
     1981 gcgagactcc cgggacctca gagtccgcca cacccgaaag tgataaaaag tattctattg
     2041 gtttagccat cggcactaat tccgttggat gggctgtcat aaccgatgaa tacaaagtac
     2101 cttcaaagaa atttaaggtg ttggggaaca cagaccgtca ttcgattaaa aagaatctta
     2161 tcggtgccct cctattcgat agtggcgaaa cggcagaggc gactcgcctg aaacgaaccg
     2221 ctcggagaag gtatacacgt cgcaagaacc gaatatgtta cttacaagaa atttttagca
     2281 atgagatggc caaagttgac gattctttct ttcaccgttt ggaagagtcc ttccttgtcg
     2341 aagaggacaa gaaacatgaa cggcacccca tctttggaaa catagtagat gaggtggcat
     2401 atcatgaaaa gtacccaacg atttatcacc tcagaaaaaa gctagttgac tcaactgata
     2461 aagcggacct gaggttaatc tacttggctc ttgcccatat gataaagttc cgtgggcact
     2521 ttctcattga gggtgatcta aatccggaca actcggatgt cgacaaactg ttcatccagt
     2581 tagtacaaac ctataatcag ttgtttgaag agaaccctat aaatgcaagt ggcgtggatg
     2641 cgaaggctat tcttagcgcc cgcctctcta aatcccgacg gctagaaaac ctgatcgcac
     2701 aattacccgg agagaagaaa aatgggttgt tcggtaacct tatagcgctc tcactaggcc
     2761 tgacaccaaa ttttaagtcg aacttcgact tagctgaaga tgccaaattg cagcttagta
     2821 aggacacgta cgatgacgat ctcgacaatc tactggcaca aattggagat cagtatgcgg
     2881 acttattttt ggctgccaaa aaccttagcg atgcaatcct cctatctgac atactgagag
     2941 ttaatactga gattaccaag gcgccgttat ccgcttcaat gatcaaaagg tacgatgaac
     3001 atcaccaaga cttgacactt ctcaaggccc tagtccgtca gcaactgcct gagaaatata
     3061 aggaaatatt ctttgatcag tcgaaaaacg ggtacgcagg ttatattgac ggcggagcga
     3121 gtcaagagga attctacaag tttatcaaac ccatattaga gaagatggat gggacggaag
     3181 agttgcttgt aaaactcaat cgcgaagatc tactgcgaaa gcagcggact ttcgacaacg
     3241 gtagcattcc acatcaaatc cacttaggcg aattgcatgc tatacttaga aggcaggagg
     3301 atttttatcc gttcctcaaa gacaatcgtg aaaagattga gaaaatccta acctttcgca
     3361 taccttacta tgtgggaccc ctggcccgag ggaactctcg gttcgcatgg atgacaagaa
     3421 agtccgaaga aacgattact ccatggaatt ttgaggaagt tgtcgataaa ggtgcgtcag
     3481 ctcaatcgtt catcgagagg atgaccaact ttgacaagaa tttaccgaac gaaaaagtat
     3541 tgcctaagca cagtttactt tacgagtatt tcacagtgta caatgaactc acgaaagtta
     3601 agtatgtcac tgagggcatg cgtaaacccg cctttctaag cggagaacag aagaaagcaa
     3661 tagtagatct gttattcaag accaaccgca aagtgacagt taagcaattg aaagaggact
     3721 actttaagaa aattgaatgc ttcgattctg tcgagatctc cggggtagaa gatcgattta
     3781 atgcgtcact tggtacgtat catgacctcc taaagataat taaagataag gacttcctgg
     3841 ataacgaaga gaatgaagat atcttagaag atatagtgtt gactcttacc ctctttgaag
     3901 atcgggaaat gattgaggaa agactaaaaa catacgctca cctgttcgac gataaggtta
     3961 tgaaacagtt aaagaggcgt cgctatacgg gctggggacg attgtcgcgg aaacttatca
     4021 acgggataag agacaagcaa agtggtaaaa ctattctcga ttttctaaag agcgacggct
     4081 tcgccaatag gaactttatg cagctgatcc atgatgactc tttaaccttc aaagaggata
     4141 tacaaaaggc acaggtttcc ggacaagggg actcattgca cgaacatatt gcgaatcttg
     4201 ctggttcgcc agccatcaaa aagggcatac tccagacagt caaagtagtg gatgagctag
     4261 ttaaggtcat gggacgtcac aaaccggaaa acattgtaat cgagatggca cgcgaaaatc
     4321 aaacgactca gaaggggcaa aaaaacagtc gagagcggat gaagagaata gaagagggta
     4381 ttaaagaact gggcagccag atcttaaagg agcatcctgt ggaaaatacc caattgcaga
     4441 acgagaaact ttacctctat tacctacaaa atggaaggga catgtatgtt gatcaggaac
     4501 tggacataaa ccgtttatct gattacgacg tcgatcacat tgtaccccaa tcctttttga
     4561 aggacgattc aatcgacaat aaagtgctta cacgctcgga taagaaccga gggaaaagtg
     4621 acaatgttcc aagcgaggaa gtcgtaaaga aaatgaagaa ctattggcgg cagctcctaa
     4681 atgcgaaact gataacgcaa agaaagttcg ataacttaac taaagctgag aggggtggct
     4741 tgtctgaact tgacaaggcc ggatttatta aacgtcagct cgtggaaacc cgccaaatca
     4801 caaagcatgt tgcacagata ctagattccc gaatgaatac gaaatacgac gagaacgata
     4861 agctgattcg ggaagtcaaa gtaatcactt taaagtcaaa attggtgtcg gacttcagaa
     4921 aggattttca attctataaa gttagggaga taaataacta ccaccatgcg cacgacgctt
     4981 atcttaatgc cgtcgtaggg accgcactca ttaagaaata cccgaagcta gaaagtgagt
     5041 ttgtgtatgg tgattacaaa gtttatgacg tccgtaagat gatcgcgaaa agcgaacagg
     5101 agataggcaa ggctacagcc aaatacttct tttattctaa cattatgaat ttctttaaga
     5161 cggaaatcac tctggcaaac ggagagatac gcaaacgacc tttaattgaa accaatgggg
     5221 agacaggtga aatcgtatgg gataagggcc gggacttcgc gacggtgaga aaagttttgt
     5281 ccatgcccca agtcaacata gtaaagaaaa ctgaggtgca gaccggaggg ttttcaaagg
     5341 aatcgattct tccaaaaagg aatagtgata agctcatcgc tcgtaaaaag gactgggacc
     5401 cgaaaaagta cggtggcttc gatagcccta cagttgccta ttctgtccta gtagtggcaa
     5461 aagttgagaa gggaaaatcc aagaaactga agtcagtcaa agaattattg gggataacga
     5521 ttatggagcg ctcgtctttt gaaaagaacc ccatcgactt ccttgaggcg aaaggttaca
     5581 aggaagtaaa aaaggatctc ataattaaac taccaaagta tagtctgttt gagttagaaa
     5641 atggccgaaa acggatgttg gctagcgccg gagagcttca aaaggggaac gaactcgcac
     5701 taccgtctaa atacgtgaat ttcctgtatt tagcgtccca ttacgagaag ttgaaaggtt
     5761 cacctgaaga taacgaacag aagcaacttt ttgttgagca gcacaaacat tatctcgacg
     5821 aaatcataga gcaaatttcg gaattcagta agagagtcat cctagctgat gccaatctgg
     5881 acaaagtatt aagcgcatac aacaagcaca gggataaacc catacgtgag caggcggaaa
     5941 atattatcca tttgtttact cttaccaacc tcggcgctcc agccgcattc aagtattttg
     6001 acacaacgat agatcgcaaa cgatacactt ctaccaagga ggtgctagac gcgacactga
     6061 ttcaccaatc catcacggga ttatatgaaa ctcggataga tttgtcacag cttgggggtg
     6121 actctggtgg ttctactaat ctgtcagata ttattgaaaa ggagaccggt aagcaactgg
     6181 ttatccagga atccatcctc atgctcccag aggaggtgga agaagtcatt gggaacaagc
     6241 cggaaagcga tatactcgtg cacaccgcct acgacgagag caccgacgag aatgtcatgc
     6301 ttctgactag cgacgcccct gaatacaagc cttgggctct ggtcatacag gatagcaacg
     6361 gtgagaacaa gattaagatg ctctctggtg gttctcccaa gaagaagagg aaagtctaac
     6421 cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga ctgtgccttc
     6481 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     6541 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     6601 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     6661 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     6721 gggctcgata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     6781 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6841 taaagcctag ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6901 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6961 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7021 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7081 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7141 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7201 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7261 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7321 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7381 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7441 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7501 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7561 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7621 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7681 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7741 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7801 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7861 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7921 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7981 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8041 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8101 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8161 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8221 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8281 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8341 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8401 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8461 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8521 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8581 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8641 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8701 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8761 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8821 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8881 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatcg
     8941 atctcccgat cccctagggt cgactctcag tacaatctgc tctgatgccg catagttaag
     9001 cca
//