| 质粒名称: | pHybrid-mTagBFP2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000204 |
| 产品用途: | Dual-expression vector. Expresses mTagBFP2 in bacterial cells and co-expresses mTagBFP2 with mScarlet in mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pHybrid |
| 表达系统: | 哺乳动物表达、细菌表达 |
| 启动子: | rhaB, CMV, SV40 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | mTagBFP2, mScarlet |
| 物种来源: | Synthetic, Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Dual-expression system for blue fluorescent protein optimization
Papadaki S., Wang X., Wang Y., Zhang H., Jia S., Liu S., Yang M., Zhang D., Jia J., Köster R.W., Namikawa K., Piatkevich K.D.
Sci. Reports 2022 121 12, 1–16 (2022)
|
买家导航
pHybrid-mTagBFP2载体质粒基本信息
pHybrid-mTagBFP2质粒图谱载体图谱和pHybrid-mTagBFP2载体序列质粒序列多克隆位点信息
pHybrid-mTagBFP2质粒载体简介
pHybrid-mTagBFP2质粒序列载体序列
LOCUS pHybrid-mTagBFP2 5597 bp DNA circular SYN 31-MAY-2023
DEFINITION 72.Electra1TSN20211022-0571-00003_1023-ban2_H09_H09_i7.1.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5597)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5597
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 754..858
/gene="bla"
/label=AmpR promoter
CDS 859..1719
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1890..2478
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
enhancer 2689..3068
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3069..3272
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 3305..3423
/label=rhaB promoter
/note="promoter of the E. coli rhaBAD operon, conferring
tight induction with L-rhamnose and repression with
D-glucose in the presence of RhaR and RhaS (Giacalone et
al., 2006)"
misc_feature 3441..3446
/label=Shine-Dalgarno
CDS 3464..3481
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind 3487..3536
/label=1216-pCI-mTagBFP2-F
primer_bind 3487..3520
/label=1216-pCI-mBlueberry2-F
regulatory 3512..3521
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 3518..4219
/codon_start=1
/product="monomeric blue fluorescent protein"
/label=mTagBFP2
/note="mammalian codon-optimized"
/translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
DLPSKLGHKLN"
primer_bind 3518..3555
/label=mTagBFP2-FW-BglII
primer_bind complement(4183..4219)
/label=mTagBFP2-RV-EcoRI-HindIII
primer_bind complement(4189..4243)
/label=1216-pCI-mTagBFP2-R
primer_bind complement(4217..4243)
/label=1216-pCI-mBlueberry2-R
terminator 4240..4326
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
polyA_signal 4350..4471
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 4512..4722
/label=SV40 promoter
/note="SV40 early promoter"
promoter 4534..4730
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 4581..4716
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4730..4749
/label=1216-pCI-mBlueberry2-F
regulatory 4731..4740
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 4737..5432
/codon_start=1
/product="bright monomeric red fluorescent protein evolved
from a synthetic template (Bindels et al., 2016)"
/label=mScarlet-I
/note="matures faster than mScarlet"
/translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
STGGMDELYK"
primer_bind complement(5412..5433)
/label=1216-pCI-mBlueberry2-R
polyA_signal 5437..5484
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin join(5564..5597,1..422)
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
61 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
121 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
181 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
241 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
301 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
361 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
421 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
481 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
541 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
601 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
661 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
721 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
781 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
841 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
901 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
961 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
1021 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
1081 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
1141 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
1201 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
1261 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
1321 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
1381 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
1441 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
1501 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
1561 gccggtgagc gtggctctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
1621 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
1681 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
1741 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
1801 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
1861 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
1921 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
1981 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
2041 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
2101 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
2161 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
2221 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
2281 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
2341 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
2401 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
2461 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
2521 tggccttttg ctcacatggc tcgacagatc ttcaatattg gccattagcc atattattca
2581 ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatctatatc
2641 ataatatgta catttatatt ggctcatgtc caatatgacc gccatgttgg cattgattat
2701 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
2761 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
2821 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
2881 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
2941 tgccaagtcc gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
3001 agtacatgac cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
3061 ttaccatggt gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac
3121 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
3181 aacgggactt tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc
3241 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcactagaa
3301 gcttcaccac aattcagcaa attgtgaaca tcatcacgtt catctttccc tggttgccaa
3361 tggcccattt tcctgtcagt aacgagaagg tcgcgaattc aggcgctttt tagactggtc
3421 gtaacccgtt tttggctaac aggaggaatt aaccatgggg agccaccatc accatcacca
3481 tgctagcctc gagaattcac gcgtggtacc agccaccatg agcgagctga ttaaggagaa
3541 catgcacatg aagctgtaca tggagggcac cgtggacaac catcacttca agtgcacatc
3601 cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg
3661 cggccctctc cccttcgcct tcgacatcct ggctactagc ttcctctacg gcagcaagac
3721 cttcatcaac cacacccagg gcatccccga cttcttcaag cagtccttcc ctgagggctt
3781 cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac
3841 cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcacatc
3901 caacggccct gtgatgcaga agaaaacact cggctgggag gccttcaccg agacgctgta
3961 ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag
4021 ccatctgatc gcaaacgcca agaccacata tagatccaag aaacccgcta agaacctcaa
4081 gatgcctggc gtctactatg tggactacag actggaaaga atcaaggagg ccaacaacga
4141 gacctacgtc gagcagcacg aggtggcagt ggccagatac tgcgacctcc ctagcaaact
4201 ggggcacaag cttaattaat ctagagtcga cccaggcatc aaataaaacg aaaggctcag
4261 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg
4321 acaaatgcgg ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac
4381 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
4441 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgggtgt ggaaagtccc
4501 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
4561 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
4621 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
4681 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gccaccatgg
4741 tgagcaaggg cgaggcagtg atcaaggagt tcatgcggtt caaggtgcac atggagggct
4801 ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
4861 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttctcc tgggacatcc
4921 tgtcccctca gttcatgtac ggctccaggg ccttcatcaa gcaccccgcc gacatccccg
4981 actactataa gcagtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
5041 acggcggcgc cgtgaccgtg acccaggaca cctccctgga ggacggcacc ctgatctaca
5101 aggtgaagct ccgcggcacc aacttccctc ctgacggccc cgtaatgcag aagaagacaa
5161 tgggctggga agcgtccacc gagcggttgt accccgagga cggcgtgctg aagggcgaca
5221 ttaagatggc cctgcgcctg aaggacggcg gccgctacct ggcggacttc aagaccacct
5281 acaaggccaa gaagcccgtg cagatgcccg gcgcctacaa cgtcgaccgc aagttggaca
5341 tcacctccca caacgaggac tacaccgtgg tggaacagta cgaacgctcc gagggccgcc
5401 actccaccgg cggcatggac gagctgtaca agtgaccggc aataaaaaga cagaataaaa
5461 cgcacggtgt tgggtcgttt gttcggatcc gggctggcgt aatagcgaag aggcccgcac
5521 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc
5581 gcattaagcg cggcggg
//
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