| 质粒名称: | pHIV-Luc-ZsGreen |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 39196 |
| 存储实验室: | Bryan Welm |
|
载体骨架基本信息 |
|
| 载体名称: | pHIV-Zsgreen |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 9312 bp |
| 载体修饰: | 将萤火虫荧光素酶Luc2P克隆到pHIV-ZsGreen（Addgene质粒18121）的EcoRI和XbaI位点，得到该质粒。 |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Firefly Luciferase Luc2P |
| 物种来源: | -- |
| 插入大小: | 1600 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | EF1-alpha |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI(未破坏) |
| 3' 克隆位点: | XbaI(未破坏) |
| 5' 测序引物 | EF1a Fwd Primer |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHIV-Luc-ZsGreen was a gift from Bryan Welm (Addgene plasmid # 39196 ; http://n2t.net/addgene:39196 ; RRID:Addgene_39196) |
| 相关载体: |
pHIV-EGFP pHIV-ZsGreen pHIV-dTomato pHIV-H2BmRFP pHIV-Luciferase pHIV-Luc-ZsGreen pHIV-iRFP720-E2A-Luc |
买家导航
pHIV-Luc-ZsGreen载体质粒基本信息
pHIV-Luc-ZsGreen质粒图谱载体图谱和pHIV-Luc-ZsGreen载体序列质粒序列多克隆位点信息
pHIV-Luc-ZsGreen质粒载体简介
pHIV-Luc-ZsGreen质粒序列载体序列
LOCUS pHIV-Luc-ZsGreen 9321 bp ds-DNA circular SYN 18-SEP-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pHIV-Luc-ZsGreen
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9321)
TITLE pHIV Lentiviruses
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9321)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 18, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9321
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 382..580
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 598..778
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 825..950
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1447..1680
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2207..2324
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2387..3565
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2618..3556
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3513..3533
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 3586..5238
/codon_start=1
/product="firefly luciferase"
/label=luciferase
/note="synthetic luc2 version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
misc_feature 5268..5854
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(5435..5452)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 5662..5681
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 5855..6550
/codon_start=1
/product="Zoanthus green fluorescent protein"
/label=ZsGreen1
/note="mammalian codon-optimized"
/translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
AIASGSALP"
primer_bind complement(6564..6580)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6565..6581)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 7090..7270
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
rep_origin complement(7332..7920)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7412..7431)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8091..8951)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8714..8733
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8952..9056)
/gene="bla"
/label=AmpR promoter
primer_bind complement(9131..9150)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
1261 acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg
1321 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
1381 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
1441 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
1501 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
1561 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
1621 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
1681 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
1741 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
1801 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
1861 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
1921 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
1981 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
2041 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
2101 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
2161 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
2221 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2281 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2341 acagcagaga tccagtttgg ttagtaccgg gcccgctcta gcgtgaggct ccggtgcccg
2401 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
2461 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
2521 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
2581 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
2641 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
2701 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
2761 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
2821 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
2881 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
2941 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
3001 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
3061 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
3121 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
3181 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
3241 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
3301 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
3361 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
3421 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
3481 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
3541 ttttcttcca tttcaggtgt cgtgagcggc cgctgaactg aattcatgga agatgccaaa
3601 aacattaaga agggcccagc gccattctac ccactcgaag acgggaccgc cggcgagcag
3661 ctgcacaaag ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt taccgacgca
3721 catatcgagg tggacattac ctacgccgag tacttcgaga tgagcgttcg gctggcagaa
3781 gctatgaagc gctatgggct gaatacaaac catcggatcg tggtgtgcag cgagaatagc
3841 ttgcagttct tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt ggccccagct
3901 aacgacatct acaacgagcg cgagctgctg aacagcatgg gcatcagcca gcccaccgtc
3961 gtattcgtga gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa gctaccgatc
4021 atacaaaaga tcatcatcat ggatagcaag accgactacc agggcttcca aagcatgtac
4081 accttcgtga cttcccattt gccacccggc ttcaacgagt acgacttcgt gcccgagagc
4141 ttcgaccggg acaaaaccat cgccctgatc atgaacagta gtggcagtac cggattgccc
4201 aagggcgtag ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc ccgcgacccc
4261 atcttcggca accagatcat ccccgacacc gctatcctca gcgtggtgcc atttcaccac
4321 ggcttcggca tgttcaccac gctgggctac ttgatctgcg gctttcgggt cgtgctcatg
4381 taccgcttcg aggaggagct attcttgcgc agcttgcaag actataagat tcaatctgcc
4441 ctgctggtgc ccacactatt tagcttcttc gctaagagca ctctcatcga caagtacgac
4501 ctaagcaact tgcacgagat cgccagcggc ggggcgccgc tcagcaagga ggtaggtgag
4561 gccgtggcca aacgcttcca cctaccaggc atccgccagg gctacggcct gacagaaaca
4621 accagcgcca ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt aggcaaggtg
4681 gtgcccttct tcgaggctaa ggtggtggac ttggacaccg gtaagacact gggtgtgaac
4741 cagcgcggcg agctgtgcgt ccgtggcccc atgatcatga gcggctacgt taacaacccc
4801 gaggctacaa acgctctcat cgacaaggac ggctggctgc acagcggcga catcgcctac
4861 tgggacgagg acgagcactt cttcatcgtg gaccggctga agagcctgat caaatacaag
4921 ggctaccagg tagccccagc cgaactggag agcatcctgc tgcaacaccc caacatcttc
4981 gacgccgggg tcgccggcct gcccgacgac gatgccggcg agctgcccgc cgcagtcgtc
5041 gtgctggaac acggtaaaac catgaccgag aaggagatcg tggactatgt ggccagccag
5101 gttacaaccg ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt gcctaaagga
5161 ctgaccggca agttggacgc ccgcaagatc cgcgagattc tcattaaggc caagaagggc
5221 ggcaagatcg ccgtgtaata attctagagt acccgggcta ggatccgccc ctctccctcc
5281 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
5341 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
5401 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
5461 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
5521 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
5581 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
5641 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
5701 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtacac atgctttaca
5761 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
5821 ctttgaaaaa cacgatgata atatggccac aaccatggcc cagtccaagc acggcctgac
5881 caaggagatg accatgaagt accgcatgga gggctgcgtg gacggccaca agttcgtgat
5941 caccggcgag ggcatcggct accccttcaa gggcaagcag gccatcaacc tgtgcgtggt
6001 ggagggcggc cccttgccct tcgccgagga catcttgtcc gccgccttca tgtacggcaa
6061 ccgcgtgttc accgagtacc cccaggacat cgtcgactac ttcaagaact cctgccccgc
6121 cggctacacc tgggaccgct ccttcctgtt cgaggacggc gccgtgtgca tctgcaacgc
6181 cgacatcacc gtgagcgtgg aggagaactg catgtaccac gagtccaagt tctacggcgt
6241 gaacttcccc gccgacggcc ccgtgatgaa gaagatgacc gacaactggg agccctcctg
6301 cgagaagatc atccccgtgc ccaagcaggg catcttgaag ggcgacgtga gcatgtacct
6361 gctgctgaag gacggtggcc gcttgcgctg ccagttcgac accgtgtaca aggccaagtc
6421 cgtgccccgc aagatgcccg actggcactt catccagcac aagctgaccc gcgaggaccg
6481 cagcgacgcc aagaaccaga agtggcacct gaccgagcac gccatcgcct ccggctccgc
6541 cttgccctaa ggatcggccg catcgatacc gtcgacctcg atcgagacct agaaaaacat
6601 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
6661 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
6721 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
6781 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
6841 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
6901 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
6961 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
7021 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
7081 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
7141 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
7201 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
7261 atctctagca gcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
7321 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
7381 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
7441 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
7501 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
7561 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
7621 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
7681 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
7741 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
7801 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
7861 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
7921 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
7981 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
8041 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
8101 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
8161 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
8221 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
8281 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
8341 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
8401 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
8461 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
8521 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
8581 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
8641 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
8701 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
8761 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
8821 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
8881 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
8941 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
9001 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
9061 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
9121 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
9181 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
9241 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
9301 tacgggccag atatacgcgt t
//
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