pAAVLINK-EF1a-NOTCH1-A

LOCUS       pAAVLINK-EF1a-NOTCH1-A      6039 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 8, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        158..700
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    505..510
                     /label=SDS
     CDS             713..3179
                     /codon_start=1
                     /label=NOTCH1
                     /translation="MPPLLAPLLCLALLPALAARGPRCSQPGETCLNGGKCEAANGTEA
                     CVCGGAFVGPRCQDPNPCLSTPCKNAGTCHVVDRRGVADYACSCALGFSGPLCLTPLDN
                     ACLTNPCRNGGTCDLLTLTEYKCRCPPGWSGKSCQQADPCASNPCANGGQCLPFEASYI
                     CHCPPSFHGPTCRQDVNECGQKPGLCRHGGTCHNEVGSYRCVCRATHTGPNCERPYVPC
                     SPSPCQNGGTCRPTGDVTHECACLPGFTGQNCEENIDDCPGNNCKNGGACVDGVNTYNC
                     RCPPEWTGQYCTEDVDECQLMPNACQNGGTCHNTHGGYNCVCVNGWTGEDCSENIDDCA
                     SAACFHGATCHDRVASFYCECPHGRTGLLCHLNDACISNPCNEGSNCDTNPVNGKAICT
                     CPSGYTGPACSQDVDECSLGANPCEHAGKCINTLGSFECQCLQGYTGPRCEIDVNECVS
                     NPCQNDATCLDQIGEFQCICMPGYEGVHCEVNTDECASSPCLHNGRCLDKINEFQCECP
                     TGFTGHLCQYDVDECASTPCKNGAKCLDGPNTYTCVCTEGYTGTHCEVDIDECDPDPCH
                     YGSCKDGVATFTCLCRPGYTGHHCETNINECSSQPCRHGGTCQDRDNAYLCFCLKGTTG
                     PNCEINLDDCASSPCDSGTCLDKIDGYECACEPGYTGSMCNINIDECAGNPCHNGGTCE
                     DGINGFTCRCPEGYHDPTCLSEVNECNSNPCVHGACRDSLNGYKCDCDPGWSGTNCDIN
                     NNECESNPCVNGGTCKDMTSGYVCTCREGFSGPNCQTNINECASNPCLNQGTCIDDVAG
                     YKCNCLLPYT"
     misc_feature    1191..1196
                     /label=SDS
     misc_feature    1776..1781
                     /label=SDS
     intron          3180..3220
                     /label=Splicing Donor
     misc_feature    3224..3257
                     /label=loxP-JT1571
     repeat_region   3302..3442
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3302..3431
                     /label=AAV2 ITR
     rep_origin      3517..3972
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4254..4358
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4359..5219
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5390..5978
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgccgcc
      721 gctcctggcg cccctgctct gcctggcgct gctgcccgcg ctcgccgcac gaggcccgcg
      781 atgctcccag cccggtgaga cctgcctgaa tggcgggaag tgtgaagcgg ccaatggcac
      841 ggaggcctgc gtctgtggcg gggccttcgt gggcccgcga tgccaggacc ccaacccgtg
      901 cctcagcacc ccctgcaaga acgccgggac atgccacgtg gtggaccgca gaggcgtggc
      961 agactatgcc tgcagctgtg ccctgggctt ctctgggccc ctctgcctga cacccctgga
     1021 caatgcctgc ctcaccaacc cctgccgcaa cgggggcacc tgcgacctgc tcacgctgac
     1081 ggagtacaag tgccgctgcc cgcccggctg gtcagggaaa tcgtgccagc aggctgaccc
     1141 gtgcgcctcc aacccctgcg ccaacggtgg ccagtgcctg cccttcgagg cctcctacat
     1201 ctgccactgc ccacccagct tccatggccc cacctgccgg caggatgtca acgagtgtgg
     1261 ccagaagccc gggctttgcc gccacggagg cacctgccac aacgaggtcg gctcctaccg
     1321 ctgcgtctgc cgcgccaccc acactggccc caactgcgag cggccctacg tgccctgcag
     1381 cccctcgccc tgccagaacg ggggcacctg ccgccccacg ggcgacgtca cccacgagtg
     1441 tgcctgcctg ccaggcttca ccggccagaa ctgtgaggaa aatatcgacg attgtccagg
     1501 aaacaactgc aagaacgggg gtgcctgtgt ggacggcgtg aacacctaca actgccgctg
     1561 cccgccagag tggacaggtc agtactgtac cgaggatgtg gacgagtgcc agctgatgcc
     1621 aaatgcctgc cagaacggcg ggacctgcca caacacccac ggtggctaca actgcgtgtg
     1681 tgtcaacggc tggactggtg aggactgcag cgagaacatt gatgactgtg ccagcgccgc
     1741 ctgcttccac ggcgccacct gccatgaccg tgtggcctcc ttctactgcg agtgtcccca
     1801 tggccgcaca ggtctgctgt gccacctcaa cgacgcatgc atcagcaacc cctgtaacga
     1861 gggctccaac tgcgacacca accctgtcaa tggcaaggcc atctgcacct gcccctcggg
     1921 gtacacgggc ccggcctgca gccaggacgt ggatgagtgc tcgctgggtg ccaacccctg
     1981 cgagcatgcg ggcaagtgca tcaacacgct gggctccttc gagtgccagt gtctgcaggg
     2041 ctacacgggc ccccgatgcg agatcgacgt caacgagtgc gtctcgaacc cgtgccagaa
     2101 cgacgccacc tgcctggacc agattgggga gttccagtgc atctgcatgc ccggctacga
     2161 gggtgtgcac tgcgaggtca acacagacga gtgtgccagc agcccctgcc tgcacaatgg
     2221 ccgctgcctg gacaagatca atgagttcca gtgcgagtgc cccacgggct tcactgggca
     2281 tctgtgccag tacgatgtgg acgagtgtgc cagcaccccc tgcaagaatg gtgccaagtg
     2341 cctggacgga cccaacactt acacctgtgt gtgcacggaa gggtacacgg ggacgcactg
     2401 cgaggtggac atcgatgagt gcgaccccga cccctgccac tacggctcct gcaaggacgg
     2461 cgtcgccacc ttcacctgcc tctgccgccc aggctacacg ggccaccact gcgagaccaa
     2521 catcaacgag tgctccagcc agccctgccg ccacgggggc acctgccagg accgcgacaa
     2581 cgcctacctc tgcttctgcc tgaaggggac cacaggaccc aactgcgaga tcaacctgga
     2641 tgactgtgcc agcagcccct gcgactcggg cacctgtctg gacaagatcg atggctacga
     2701 gtgtgcctgt gagccgggct acacagggag catgtgtaac atcaacatcg atgagtgtgc
     2761 gggcaacccc tgccacaacg ggggcacctg cgaggacggc atcaatggct tcacctgccg
     2821 ctgccccgag ggctaccacg accccacctg cctgtctgag gtcaatgagt gcaacagcaa
     2881 cccctgcgtc cacggggcct gccgggacag cctcaacggg tacaagtgcg actgtgaccc
     2941 tgggtggagt gggaccaact gtgacatcaa caacaatgag tgtgaatcca acccttgtgt
     3001 caacggcggc acctgcaaag acatgaccag tggctacgtg tgcacctgcc gggagggctt
     3061 cagcggtccc aactgccaga ccaacatcaa cgagtgtgcg tccaacccat gtctgaacca
     3121 gggcacgtgt attgacgacg ttgccgggta caagtgcaac tgcctgctgc cctacacagg
     3181 taagtatcaa ggttacaaga caggtttaag gagaccaata agctattatt cgtatagcat
     3241 acattatacg aagttattaa gttatggaga ccggtgtggc acgtgcggac cgagcggccg
     3301 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     3361 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     3421 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
     3481 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     3541 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc
     3601 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     3661 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     3721 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     3781 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     3841 cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct
     3901 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     3961 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     4021 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     4081 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     4141 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     4201 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     4261 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4321 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4381 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4441 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4501 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4561 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4621 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4681 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4741 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4801 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4861 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4921 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4981 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5041 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5101 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5161 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5221 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5281 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5341 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5401 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5461 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5521 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5581 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5641 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5701 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5761 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5821 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5881 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5941 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6001 tacggttcct ggccttttgc tggccttttg ctcacatgt
//