| 质粒名称: | pUC18-mini-Tn7T-Gm-lux |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 64963 |
| 存储实验室: | Herbert Schweizer |
|
载体骨架基本信息 |
|
| 载体名称: | pUC18-mini-Tn7T-Gm |
| 载体出品公司: | Herbert Schweizer |
| 载体抗性: | 庆大霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | lux operon |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pUC18-mini-Tn7T-Gm-lux was a gift from Herbert Schweizer (Addgene plasmid # 64963 ; http://n2t.net/addgene:64963 ; RRID:Addgene_64963) |
| 参考文献部分: | A Tn7-based broad-range bacterial cloning and expression system. Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer RR, Schweizer HP. Nat Methods. 2005 Jun;2(6):443-8. 10.1038/nmeth765 PubMed 15908923 |
买家导航
pUC18-mini-Tn7T-Gm-lux载体质粒基本信息
pUC18-mini-Tn7T-Gm-lux质粒图谱载体图谱和pUC18-mini-Tn7T-Gm-lux载体序列质粒序列多克隆位点信息
pUC18-mini-Tn7T-Gm-lux质粒载体简介
pUC18-mini-Tn7T-Gm-lux质粒序列载体序列
LOCUS pUC18-mini-Tn7T-Gm-lux 11299 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION mini-Tn7 luxCDABE transcriptional fusion vector.
ACCESSION .
VERSION .
KEYWORDS pUC18-mini-Tn7T-Gm-lux
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11299)
AUTHORS Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer
RR, Schweizer HP
TITLE A Tn7-based broad-range bacterial cloning and expression system.
JOURNAL Nat Methods. 2005 Jun;2(6):443-8.
PUBMED 15908923
REFERENCE 2 (bases 1 to 11299)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11299
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(29..51)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 151..170
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 584..600
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 585..601
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
terminator 629..723
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
terminator 826..912
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
protein_bind 958..1005
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
CDS complement(1135..1668)
/codon_start=1
/gene="aacC1"
/product="gentamycin acetyltransferase"
/label=GmR
/note="confers resistance to gentamycin"
/translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
EEVMHFDIDPSTAT"
promoter complement(1857..1885)
/gene="intI1 (promoter lies within the coding sequence)"
/label=Pc promoter
/note="class 1 integron promoter"
protein_bind 1926..1973
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
CDS 2517..3959
/codon_start=1
/gene="luxC"
/product="LuxC fatty acid reductase"
/label=LuxC
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
DVAVEIEQTRFLEEDKFLVFVP"
CDS 3971..4894
/codon_start=1
/gene="luxD"
/product="LuxD acyltransferase"
/label=LuxD
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
FEHLTIATVNERRMRIEIENQAISLS"
CDS 4943..6025
/codon_start=1
/gene="luxA"
/product="LuxA luciferase subunit"
/label=LuxA
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
KLFQSDVMPFLKEKQRSLLY"
CDS 6040..7023
/codon_start=1
/gene="luxB"
/product="LuxB luciferase subunit"
/label=LuxB
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVAIAEEACLLDQLSEGRFILG
FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
CDS 7202..8314
/codon_start=1
/gene="luxE"
/product="LuxE"
/label=LuxE
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
mobile_element 9045..9210
/mobile_element_type="transposon:Tn7"
/note="mini-Tn7 element (left end of the Tn7 transposon)"
primer_bind complement(9309..9326)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9480..10068)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9560..9579)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10239..11099)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10862..10881
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11100..11204)
/gene="bla"
/label=AmpR promoter
primer_bind 11272..11290
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct agaggaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa
361 tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga
421 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
481 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
541 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggttcgaggt cgacggtatc
601 gataagctag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
661 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
721 aatccaagct agactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
781 ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag
841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
901 agtaggacaa atccgccgct aggagcttgc ggcccggacg atcgaattgg ggatcttgaa
961 gtacctattc cgaagttcct attctctaga aagtatagga acttcagagc gcttttgaag
1021 ctgatgtgct taaaaactta ctcaatggaa ttctagatcg aattggccgc ggcgttgtga
1081 caatttaccg aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt
1141 ggcggtactt gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata
1201 gagagccact gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag
1261 cgcgttggcc tcatgcttga ggagattgat gagcgcggtg gcaatgccct gcctccggtg
1321 ctcgccggag actgcgagat catagatata gatctcacta cgcggctgct caaacttggg
1381 cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat
1441 gaatgtctta ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta
1501 ggtggctacg tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac
1561 ttggtcaggg ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt
1621 tttagggcga ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc
1681 ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag
1741 actgtacaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc
1801 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagtt
1861 tacgaaccga acaggcttat gtcaattcga tccattgctg ttgacaaagg gaatcagggg
1921 atcttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc
1981 ttttgaagct aattcgatca tgcatgagct cactagtgga tcccccgggc tgcaggaatt
2041 cctcgagaag ctgatcccca attcttcttt agaaatctgc cggtaaaaat tagattgcta
2101 ttcaatctat ttctatcggt atttgtgaaa taatactcag gataataatt tacataaata
2161 ttatcacgca ttagagaaga gcatgacttt tttaatttaa acttttcatt aacaaatctt
2221 gttgatatga aaattttcct ttgctatttt aacagatatt aaaacgggaa taggcgttat
2281 attgacgatc cattcagtta gattaaaaac cttgagcaga aaatttatat tattatcata
2341 attatgacga aagttacagg ccaggaacca cgtagtcaga atctgatttt ctatatattt
2401 gttatttaca tcgtcataac acaaaaatat aagaagcaag tgttggtacg accagttcgc
2461 aagatagtta aacagcaact taagttgaaa ttacccccat taaatggatg gcaaatatga
2521 ctaaaaaaat ttcattcatt attaacggcc aggttgaaat ctttcccgaa agtgatgatt
2581 tagtgcaatc cattaatttt ggtgataata gtgtttacct gccaatattg aatgactctc
2641 atgtaaaaaa cattattgat tgtaatggaa ataacgaatt acggttgcat aacattgtca
2701 attttctcta tacggtaggg caaagatgga aaaatgaaga atactcaaga cgcaggacat
2761 acattcgtga cttaaaaaaa tatatgggat attcagaaga aatggctaag ctagaggcca
2821 attggatatc tatgatttta tgttctaaag gcggccttta tgatgttgta gaaaatgaac
2881 ttggttctcg ccatatcatg gatgaatggc tacctcagga tgaaagttat gttcgggctt
2941 ttccgaaagg taaatctgta catctgttgg caggtaatgt tccattatct gggatcatgt
3001 ctatattacg cgcaatttta actaagaatc agtgtattat aaaaacatcg tcaaccgatc
3061 cttttaccgc taatgcatta gcgttaagtt ttattgatgt agaccctaat catccgataa
3121 cgcgctcttt atctgttata tattggcccc accaaggtga tacatcactc gcaaaagaaa
3181 ttatgcgaca tgcggatgtt attgtcgctt ggggagggcc agatgcgatt aattgggcgg
3241 tagagcacgc gccatcttat gctgatgtga ttaaatttgg ttctaaaaag agtctttgca
3301 ttatcgataa tcctgttgat ttgacgtccg cagcgacagg tgcggctcat gatgtttgtt
3361 tttacgatca gcgagcttgt ttttctgccc aaaacatata ttacatggga aatcattatg
3421 aggaatttaa gttagcgttg atagaaaaac ttaatctata tgcgcatata ttaccgaatg
3481 ccaaaaaaga ttttgatgaa aaggcggcct attctttagt tcaaaaagaa agcctgtttg
3541 ctggattaaa agtagaggtg gatattcatc aacgttggat gattattgag tcaaatgcag
3601 gtgtggaatt taatcaacca cttggcagat gtgtgtacct tcatcacgtc gataatattg
3661 agcaaatatt gccttatgtt caaaaaaata agacgcaaac catatctatt tttccttggg
3721 agtcatcatt taaatatcga gatgcgttag cattaaaagg tgcggaaagg attgtagaag
3781 caggaatgaa taacatattt cgagttggtg gatctcatga cggaatgaga ccgttgcaac
3841 gattagtgac atatatttct catgaaaggc catctaacta tacggctaag gatgttgcgg
3901 ttgaaataga acagactcga ttcctggaag aagataagtt ccttgtattt gtcccataat
3961 aggtaaaagt atggaaaatg aatcaaaata taaaaccatc gaccacgtta tttgtgttga
4021 aggaaataaa aaaattcatg tttgggaaac gctgccagaa gaaaacagcc caaagagaaa
4081 gaatgccatt attattgcgt ctggttttgc ccgcaggatg gatcattttg ctggtctggc
4141 ggaatattta tcgcggaatg gatttcatgt gatccgctat gattcgcttc accacgttgg
4201 attgagttca gggacaattg atgaatttac aatgtctata ggaaagcaga gcttgttagc
4261 agtggttgat tggttaacta cacgaaaaat aaataacttc ggtatgttgg cttccagctt
4321 atctgcgcgg atagcttatg caagcctatc tgaaatcaat gcttcgtttt taatcaccgc
4381 agtcggtgtt gttaacttaa gatattctct tgaaagagct ttagggtttg attatctcag
4441 tctacccatt aatgaattgc cggataatct ggattttgaa ggccataaat tgggtgctga
4501 agtctttgcg agagattgtc ttgattttgg ttgggaagat ttagcttcta caattaataa
4561 catgatgtat cttgatatac cgtttattgc ttttactgca aataacgata attgggtcaa
4621 gcaagatgaa gttatcacat tgttatcaaa tattcgtagt aatcgatgca agatatattc
4681 tttgttagga agttcgcatg acttgagtga aaatttagtg gtcctgcgca atttttatca
4741 atcggttacg aaagccgcta tcgcgatgga taatgatcat ctggatattg atgttgatat
4801 tactgaaccg tcatttgaac atttaactat tgcgacagtc aatgaacgcc gaatgagaat
4861 tgagattgaa aatcaagcaa tttctctgtc ttaaaatcta ttgagatatt ctatcactca
4921 aatagcaata taaggactct ctatgaaatt tggaaacttt ttgcttacat accaacctcc
4981 ccaattttct caaacagagg taatgaaacg tttggttaaa ttaggtcgca tctctgagga
5041 gtgtggtttt gataccgtat ggttactgga gcatcatttc acggagtttg gtttgcttgg
5101 taacccttat gtcgctgctg catatttact tggcgcgact aaaaaattga atgtaggaac
5161 tgccgctatt gttcttccca cagcccatcc agtacgccaa cttgaagatg tgaatttatt
5221 ggatcaaatg tcaaaaggac gatttcggtt tggtatttgc cgagggcttt acaacaagga
5281 ctttcgcgta ttcggcacag atatgaataa cagtcgcgcc ttagcggaat gctggtacgg
5341 gctgataaag aatggcatga cagagggata tatggaagct gataatgaac atatcaagtt
5401 ccataaggta aaagtaaacc ccgcggcgta tagcagaggt ggcgcaccgg tttatgtggt
5461 ggctgaatca gcttcgacga ctgagtgggc tgctcaattt ggcctaccga tgatattaag
5521 ttggattata aatactaacg aaaagaaagc acaacttgag ctttataatg aagtggctca
5581 agaatatggg cacgatattc ataatatcga ccattgctta tcatatataa catctgtaga
5641 tcatgactca attaaagcga aagagatttg ccggaaattt ctggggcatt ggtatgattc
5701 ttatgtgaat gctacgacta tttttgatga ttcagaccaa acaagaggtt atgatttcaa
5761 taaagggcag tggcgtgact ttgtattaaa aggacataaa gatactaatc gccgtattga
5821 ttacagttac gaaatcaatc ccgtgggaac gccgcaggaa tgtattgaca taattcaaaa
5881 agacattgat gctacaggaa tatcaaatat ttgttgtgga tttgaagcta atggaacagt
5941 agacgaaatt attgcttcca tgaagctctt ccagtctgat gtcatgccat ttcttaaaga
6001 aaaacaacgt tcgctattat attagctaag gagaaagaaa tgaaatttgg attgttcttc
6061 cttaacttca tcaattcaac aactgttcaa gaacaaagta tagttcgtat gcaggaaata
6121 acggagtatg ttgataagtt gaattttgaa cagattttag tgtatgaaaa tcatttttca
6181 gataatggtg ttgtcggcgc tcctctgact gtttctggtt ttctgctcgg tttaacagag
6241 aaaattaaaa ttggttcatt aaatcacatc attacaactc atcatcctgt cgccatagcg
6301 gaggaagcgt gcttattgga tcagttaagt gaagggagat ttattttagg gtttagtgat
6361 tgcgaaaaaa aagatgaaat gcattttttt aatcgcccgg ttgaatatca acagcaacta
6421 tttgaagagt gttatgaaat cattaacgat gctttaacaa caggctattg taatccagat
6481 aacgattttt atagcttccc taaaatatct gtaaatcccc atgcttatac gccaggcgga
6541 cctcggaaat atgtaacagc aaccagtcat catattgttg agtgggcggc caaaaaaggt
6601 attcctctca tctttaagtg ggatgattct aatgatgtta gatatgaata tgctgaaaga
6661 tataaagccg ttgcggataa atatgacgtt gacctatcag agatagacca tcagttaatg
6721 atattagtta actataacga agatagtaat aaagctaaac aagagacgcg tgcatttatt
6781 agtgattatg ttcttgaaat gcaccctaat gaaaatttcg aaaataaact tgaagaaata
6841 attgcagaaa acgctgtcgg aaattatacg gagtgtataa ctgcggctaa gttggcaatt
6901 gaaaagtgtg gtgcgaaaag tgtattgctg tcctttgaac caatgaatga tttgatgagc
6961 caaaaaaatg taatcaatat tgttgatgat aatattaaga agtaccacat ggaatatacc
7021 taatagattt cgagttgcag cgaggcggca agtgaacgaa tccccaggag catagataac
7081 tatgtgactg gggtgagtga aagcagccaa caaagcagca gcttgaaaga tgaagggtat
7141 aaaagagtat gacagcagtg ctgccatact ttctaatatt atcttgagga gtaaaacagg
7201 tatgacttca tatgttgata aacaagaaat tacagcaagc tcagaaattg atgatttgat
7261 tttttcgagc gatccattag tgtggtctta cgacgagcag gaaaaaatca gaaagaaact
7321 tgtgcttgat gcatttcgta atcattataa acattgtcga gaatatcgtc actactgtca
7381 ggcacacaaa gtagatgaca atattacgga aattgatgac atacctgtat tcccaacatc
7441 ggtttttaag tttactcgct tattaacttc tcaggaaaac gagattgaaa gttggtttac
7501 cagtagcggc acgaatggtt taaaaagtca ggtggcgcgt gacagattaa gtattgagag
7561 actcttaggc tctgtgagtt atggcatgaa atatgttggt agttggtttg atcatcaaat
7621 agaattagtc aatttgggac cagatagatt taatgctcat aatatttggt ttaaatatgt
7681 tatgagtttg gtggaattgt tatatcctac gacatttacc gtaacagaag aacgaataga
7741 ttttgttaaa acattgaata gtcttgaacg aataaaaaat caagggaaag atctttgtct
7801 tattggttcg ccatacttta tttatttact ctgccattat atgaaagata aaaaaatctc
7861 attttctgga gataaaagcc tttatatcat aaccggaggc ggctggaaaa gttacgaaaa
7921 agaatctctg aaacgtgatg atttcaatca tcttttattt gatactttca atctcagtga
7981 tattagtcag atccgagata tatttaatca agttgaactc aacacttgtt tctttgagga
8041 tgaaatgcag cgtaaacatg ttccgccgtg ggtatatgcg cgagcgcttg atcctgaaac
8101 gttgaaacct gtacctgatg gaacgccggg gttgatgagt tatatggatg cgtcagcaac
8161 cagttatcca gcatttattg ttaccgatga tgtcgggata attagcagag aatatggtaa
8221 gtatcccggc gtgctcgttg aaattttacg tcgcgtcaat acgaggacgc agaaagggtg
8281 tgctttgagc ttaaccgaag cgtttgatag ttgatatcct ttgcctaatt gtaagtggaa
8341 tgcttgcgtt atataaatct gaatgacatc tacactttac aaaattctcc aaaacatcca
8401 catttgggta cttgatagag gtttatgggg ttggcttaac attgttctca ttgttattat
8461 tggctcaaag caaaaggaga taacatgaaa aaattggcag ttatgcttgc attgggaatg
8521 attagctttg gtgcaatggc agttgatggg tataaagatg caaagtttgg catgacagaa
8581 gaagagtttc tttcgaagag gttatgtgat tttgaaaaat ttgagggaga ttctcgaata
8641 gaagaagtat cactttattc atgttctgac ttttcgtttg ctaacaaaaa gcgtgaagca
8701 atggcatttt ttttaaatgg gaaatttaaa agattagaga ttaatattgg cagacttgtg
8761 aagccagtaa gcaaatcgtt aacgaaaaag tacggagatg gatcatcgta tccatcaaaa
8821 gaagaatttg agaacgcgct aaaatacaat ggaactatgt ctataggtta tgataataat
8881 acggtattag ttgatataca tataatatgt ggcaaagaag gcatagaaac cagtcaactg
8941 atttatacga gtccagatgt ttatacgctc ccagatttcg gagaaaaaat ccaggaatta
9001 aagggattaa aggaattggg tacctcgcga aggccttgca ggccaaccag ataagtgaaa
9061 tctagttcca aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca
9121 gttcccatct attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct
9181 cccagttccc aactattttg tccgcccaca gcggggcatt tttcttcctg ttatgtttgg
9241 gcgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
9301 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
9361 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
9421 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
9481 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
9541 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
9601 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
9661 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
9721 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
9781 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
9841 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
9901 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
9961 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
10021 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
10081 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
10141 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
10201 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
10261 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
10321 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
10381 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
10441 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
10501 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
10561 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
10621 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
10681 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
10741 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
10801 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
10861 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
10921 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
10981 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
11041 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
11101 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
11161 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
11221 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
11281 atcacgaggc cctttcgtc
//
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