| 质粒名称: | pAAV-CAG-GINKO2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002100 |
| 产品用途: | The genetically encoded green fluorescent potassium indicator GINKO2 |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCAG |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | GINKO2 |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Sensitive red fluorescent indicators for real-time visualization of potassium ion dynamics in vivo
Yang L, Pathiranage V, Zhou S, Sun X, Zhang H, Lai C, Gu C, Subach FV, Drobizhev M, Walker AR, Piatkevich KD
PLoS Biol,Issue 9,2025 Sep 17
|
pAAV-CAG-GINKO2
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-CAG-GINKO2载体质粒基本信息
pAAV-CAG-GINKO2质粒图谱载体图谱和pAAV-CAG-GINKO2载体序列质粒序列多克隆位点信息
pAAV-CAG-GINKO2质粒载体简介
pAAV-CAG-GINKO2质粒序列载体序列
LOCUS pAAV-CAG-GINKO2 5938 bp DNA circular SYN 20-MAR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5938)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 17, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5938
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(182..770)
/direction=LEFT
/label=ori
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(941..1801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(1802..1906)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1932..2387)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2942..3219
/label=chicken beta-actin promoter
primer_bind 3621..3665
/label=CAG-F1
misc_feature 3643..3648
/label=kozak
CDS 3649..4815
/codon_start=1
/label=GINKO2
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVYGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYTQERTIFFEDD
GNYKTRAEVKFEGDTLVNRIELEGIDFKEDGNILGHKLEYNLPDGLFNFVKDAGEKLWD
AVTGQHDKDDQAKKVWEHLHKTGIPDADKVNIQVADGKATVAGDGLSQEAKEKILVAVG
NISGIASVDDQVKTATPATASQFYTVKSGDTLSAISNQVYGNADLYNKIFEANKPMLKS
PDKIYPGQVLRIPYAANNVYIKADKEKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGD
GPVLLPDNHYLSVQSRLSKDPNEKRDHMVLLEFVTAAGITLGMDELYM"
primer_bind complement(3694..3715)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3955..3974)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind complement(4103..4122)
/label=S9A-R1
primer_bind complement(4303..4317)
/label=T73A-R1
primer_bind complement(4431..4455)
/label=G113V-R
primer_bind complement(4516..4530)
/label=Ec-kbp-R
primer_bind 4749..4770
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(4794..4838)
/label=CAG-R1
primer_bind complement(4816..4837)
/label=Intra-cag-R1
primer_bind complement(4816..4837)
/label=Azu-cag-R1
misc_feature 4840..5428
/label=WPRE
/note="woodchuck posttranscriptional regulatory element"
CDS complement(5311..5322)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 5471..5592
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(join(5933..5938,1..4))
/label=PA-F6A-F1
primer_bind join(5933..5938,1..4)
/label=G11A-R1
primer_bind join(5933..5938,1..4)
/label=R12A-R1
ORIGIN
1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
2641 gaagatcgta ggttaattaa ggcgcgcccc taggggagat ggccagccag cccattgacg
2701 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
2761 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
2821 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
2881 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
2941 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
3001 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
3061 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
3121 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
3181 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct
3241 tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
3301 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
3361 ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga
3421 gggccctttg tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg
3481 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
3541 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
3601 tgctgtctca tcattttggc aaagaattgg ggatccggta ccgccaccat ggtgagcaag
3661 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
3721 ggccacaagt tcagcgtgta cggcgagggt gagggcgatg ccacctacgg caagctgacc
3781 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccactct cgtgaccacc
3841 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
3901 ttcaagtccg ccatgcccga aggctacacc caggagcgca ccatcttctt cgaggacgac
3961 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
4021 gagctggagg gcatcgactt caaggaggac ggcaacattc tggggcacaa gctggagtac
4081 aacctgccgg acggcctgtt caacttcgtg aaggacgccg gcgagaagct gtgggatgcc
4141 gtgacaggcc agcacgacaa ggacgaccag gccaagaagg tgtgggaaca cctgcacaag
4201 accggcatcc ccgacgccga caaagtgaac atccaggtcg ccgacggcaa ggccacagtg
4261 gcaggcgacg gactgagcca ggaagccaaa gaaaagatcc tggtggccgt gggcaacatc
4321 agcggaatcg ccagcgtgga cgaccaagtg aaaaccgcca cccctgccac cgccagccag
4381 ttctacacag tgaagtccgg cgacaccctg agcgccatca gcaatcaggt gtacggcaac
4441 gccgacctgt acaacaagat tttcgaggcc aacaagccca tgctgaagtc ccccgacaaa
4501 atctaccccg gccaggtgct gagaatcccc tatgctgcta ataacgtcta tatcaaggcc
4561 gacaaggaga agaacggcat caaggcgaac ttcaagatcc gccacaacat cgaggacggc
4621 ggcgtgcagc tcgcctacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
4681 ctgcccgaca accactacct gagcgtgcag tccagacttt ctaaagaccc caacgagaag
4741 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
4801 gagctgtaca tgtaagaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
4861 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
4921 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
4981 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
5041 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
5101 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
5161 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
5221 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
5281 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
5341 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
5401 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacccgggcg gccgcttcga
5461 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
5521 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
5581 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg
5641 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatctt
5701 cctagagcat ggctacgtag ataagtagca tggcgggtta atcattaact acaaggaacc
5761 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
5821 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
5881 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgct
//
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