| 质粒名称: | pBAD-Flp |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 122969 |
| 存储实验室: | Lydia Freddolino |
|
载体骨架基本信息 |
|
| 载体名称: | pCP20 |
| 载体出品公司: | Clontech |
| 空载体大小: | 4038 bp |
| 完整质粒大小: | 10691bp |
| 载体类型: | 细菌表达 |
| 载体抗性: | Chloramphenicol, 25 μg/mL |
| 生长温度: | 30 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Flp recombinase |
| 物种来源: | 光合细菌 Rhodopseudomonas palustris |
| 插入大小: | 1272bp |
| 启动子: | pBAD |
| 克隆方法: | Gibson Cloning |
| 5’测序引物 |
pBAD Forward
(Common Sequencing Primers)
|
|
文献引用方法 |
|
| 材料和方法部分: | pBAD-Flp was a gift from Lydia Freddolino (Addgene plasmid # 122969 ; http://n2t.net/addgene:122969 ; RRID:Addgene_122969) |
| 参考文献部分: | High-Resolution Mapping of the Escherichia coli Chromosome Reveals Positions of High and Low Transcription. Scholz SA, Diao R, Wolfe MB, Fivenson EM, Lin XN, Freddolino PL. Cell Syst. 2019 Mar 27;8(3):212-225.e9. doi: 10.1016/j.cels.2019.02.004. Epub 2019 Mar 20. 10.1016/j.cels.2019.02.004 PubMed 30904377 |
买家导航
pBAD-Flp载体质粒基本信息
pBAD-Flp质粒图谱载体图谱和pBAD-Flp载体序列质粒序列多克隆位点信息
pBAD-Flp质粒载体简介
pBAD-Flp质粒序列载体序列
LOCUS pBAD-Flp 10691 bp DNA circular SYN 25-AUG-2024
DEFINITION Expresses flp recombinase in bacteria upon arabinose induction. No
flp activity is detected without induction..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10691)
AUTHORS Scholz SA, Diao R, Wolfe MB, Fivenson EM, Lin XN, Freddolino PL
TITLE High-Resolution Mapping of the Escherichia coli Chromosome Reveals
Positions of High and Low Transcription.
JOURNAL Cell Syst. 2019 Mar 27;8(3):212-225.e9. doi:
10.1016/j.cels.2019.02.004. Epub 2019 Mar 20.
PUBMED 30904377
REFERENCE 2 (bases 1 to 10691)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1016/j.cels.2019.02.004"; journalName: "Cell Syst"; date:
"2019-03-27- 27"; volume: "8"; issue: "3"; pages: "212-225.e9"
FEATURES Location/Qualifiers
source 1..10691
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(1083..1102)
/label=pENTR-R
/note="pENTR vectors, reverse primer"
primer_bind complement(1167..1186)
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
CDS complement(1904..2617)
/codon_start=1
/gene="cIts"
/product="temperature-sensitive variant of the phage lambda
repressor"
/label=lambda repressor (ts)
/note="thermosensitivity is conferred by the A67T mutation"
/translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
VGKVIASQWPEETFG"
CDS complement(2659..3537)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRH
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
promoter 3564..3848
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
primer_bind 3771..3790
/label=pBAD Forward
/note="For vectors with E. coli araBAD promoter, forward
primer"
CDS 3866..5137
/codon_start=1
/product="site-specific recombinase"
/label=FLP
/note="FLP is a site-specific recombinase from
Saccharomyces cerevisiae. Recombination occurs at FRT
sequences."
/translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
YPAWNGIISQEVLDYLSSYINRRI"
primer_bind complement(6085..6103)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6171..6275
/gene="bla"
/label=AmpR promoter
CDS 6276..7136
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6494..6513)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
CDS complement(8598..9548)
/codon_start=1
/gene="rep101 encoding an A56V mutation"
/product="temperature-sensitive version of the RepA protein
needed for replication with the pSC101 origin (Armstrong et
al., 1984)"
/label=Rep101(Ts)
/translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
rep_origin 9596..9818
/label=pSC101 ori
/note="low-copy replication origin that requires the Rep101
protein"
promoter 10370..10472
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS join(10473..10691,1..441)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(10539..10558)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
1 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
61 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
121 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
181 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
241 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
301 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
361 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
421 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
481 tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgaa agcaaattcg
541 acccggtcgt cggttcaggg cagggtcgtt aaatagccgc ttatgtctat tgctggttta
601 ccggtttatt gactaccgga agcagtgtga ccgtgtgctt ctcaaatgcc tgaggccagt
661 ttgctcaggc tctccccgtg gaggtaataa ttgacgatat gatcatttat tctgcctccc
721 agagcctgat aaaaacggtt agcgccagca accgcacctg tggcgctgag gtctgcctcg
781 tgaagaaggt gttgctgact cataccaggc ctgaatcgcc ccatcatcca gccagaaagt
841 gagggagcca cggttgatga gagctttgtt gtaggtggac cagttggtga ttttgaactt
901 ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc gtgatctgat ccttcaactc
961 agcaaaagtt cgatttattc aacaaagcca cgttgtgtct caaaatctct gatgttacat
1021 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
1081 tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgctcgaggc cgcgattaaa
1141 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
1201 aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
1261 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
1321 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
1381 actcaccact gcgatccccg ggaaaacagc attccaggta ttagaagaat atcctgattc
1441 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
1501 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
1561 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
1621 acaagtctgg aaagaaatgc agattcttgc tcaattgtta tcagctatgc gccgaccaga
1681 acaccttgcc gatcagccaa acgtctcttc aggccactga ctagcgataa ctttccccac
1741 aacggaacaa ctctcattgc atgggatcat tgrrtamcgg tttggttgat gcgagtgatt
1801 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cagattcttg
1861 ctcaattgtt atcagctatg cgccgaccag aacaccttgc cgatcagcca aacgtctctt
1921 caggccactg actagcgata actttcccca caacggaaca actctcattg catgggatca
1981 ttgggtactg tgggtttagt ggttgtaaaa acacctgacc gctatccctg atcagtttct
2041 tgaaggtaaa ctcatcaccc ccaagtctgg ctatgcagaa atcacctggc tcaacagcct
2101 gctcagggtc aacgagaatt aacattccgt caggaaagct tggcttggag cctgttggtg
2161 cggtcatgga attaccttca acctcaagcc agaatgcaga atcactggct tttttggttg
2221 tgcttaccca tctctccgca tcacctttgg taaaggttct aagcttaggt gagaacatcc
2281 ctgcctgaac atgagaaaaa acagggtact catactcact tctaagtgac ggctgcatac
2341 taaccgcttc atacatctcg tagatttctc tggcgattga agggctaaat tcttcaacgc
2401 taactttgag aatttttgta agcaatgcgg cgttataagc atttaatgca ttgatgccat
2461 taaataaagc accaacgcct gactgcccca tccccatctt gtctgcgaca gattcctggg
2521 ataagccaag ttcatttttc tttttttcat aaattgcttt aaggcgacgt gcgtcctcaa
2581 gctgctcttg tgttaatggt ttcttttttg tgctcatacg ttaaatctat caccgcaagg
2641 gataaatatc taacaccgtt atgacaactt gacggctaca tcattcactt tttcttcaca
2701 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaagta
2761 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct
2821 caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc
2881 taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat gctgtgcgac
2941 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg
3001 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgt gccgcagtaa
3061 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc
3121 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa
3181 gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg
3241 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg
3301 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg
3361 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag
3421 cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac
3481 cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatact
3541 tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca gacattgccg
3601 tcactgcgtc ttttactggc tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag
3661 cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa
3721 tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc
3781 atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac tctctactgt
3841 ttctccatac ccgttaggag gttgtatgcc acaatttgat atattatgta aaacaccacc
3901 taaggtgctt gttcgtcagt ttgtggaaag gtttgaaaga ccttcaggtg agaaaatagc
3961 attatgtgct gctgaactaa cctatttatg ttggatgatt acacataacg gaacagcaat
4021 caagagagcc acattcatga gctataatac tatcataagc aattcgctga gtttcgatat
4081 tgtcaataaa tcactccagt ttaaatacaa gacgcaaaaa gcaacaattc tggaagcctc
4141 attaaagaaa ttgattcctg cttgggaatt tacaattatt ccttactatg gacaaaaaca
4201 tcaatctgat atcactgata ttgtaagtag tttgcaatta cagttcgaat catcggaaga
4261 agcagataag ggaaatagcc acagtaaaaa aatgcttaaa gcacttctaa gtgagggtga
4321 aagcatctgg gagatcactg agaaaatact aaattcgttt gagtatactt cgagatttac
4381 aaaaacaaaa actttatacc aattcctctt cctagctact ttcatcaatt gtggaagatt
4441 cagcgatatt aagaacgttg atccgaaatc atttaaatta gtccaaaata agtatctggg
4501 agtaataatc cagtgtttag tgacagagac aaagacaagc gttagtaggc acatatactt
4561 ctttagcgca aggggtagga tcgatccact tgtatatttg gatgaatttt tgaggaattc
4621 tgaaccagtc ctaaaacgag taaataggac cggcaattct tcaagcaata aacaggaata
4681 ccaattatta aaagataact tagtcagatc gtacaataaa gctttgaaga aaaatgcgcc
4741 ttattcaatc tttgctataa aaaatggccc aaaatctcac attggaagac atttgatgac
4801 ctcatttctt tcaatgaagg gcctaacgga gttgactaat gttgtgggaa attggagcga
4861 taagcgtgct tctgccgtgg ccaggacaac gtatactcat cagataacag caatacctga
4921 tcactacttc gcactagttt ctcggtacta tgcatatgat ccaatatcaa aggaaatgat
4981 agcattgaag gatgagacta atccaattga ggagtggcag catatagaac agctaaaggg
5041 tagtgctgaa ggaagcatac gataccccgc atggaatggg ataatatcac aggaggtact
5101 agactacctt tcatcctaca taaatagacg catataagta cgcatttaag cataaacacg
5161 cactatgccg ttcttctcat gtatatatat atacaggcaa cacgcagata taggtgcgac
5221 gtgaacagtg agctgtatgt gcgcagctcg cgttgcattt tcggaagcgc tcgttttcgg
5281 aaacgctttg aagttcctat tccgaagttc ctattctgcc attctcaccg gattcagtcg
5341 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
5401 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
5461 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
5521 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag
5581 aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcggct
5641 ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa
5701 cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa
5761 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcctg
5821 gtatgagtca gcaacacctt cttcacgagg cagacctcag cgccggtgat gccggccacg
5881 atgcgtccgg cgtagaggat cctagagcgc acgaatgagg gccgacagga agcaaagctg
5941 aaaggaatca aatttggccg caggcgtacc gtggacagga acgtcgtgct gacgcttcat
6001 cagaagggca ctggtgcaac ggaaattgct catcagctca gtattgcccg ctccacggtt
6061 tataaaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
6121 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
6181 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
6241 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
6301 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
6361 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
6421 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
6481 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
6541 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
6601 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
6661 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
6721 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
6781 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
6841 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
6901 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
6961 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
7021 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
7081 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
7141 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
7201 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
7261 cgttccactg agcgtcagac cccgtttagt cactgatgcc tccgtgtaag ggggatttct
7321 gttcatgggg taatgatacc gatgaaacgc gagaggatgc tcacaatacg ggttactgat
7381 gatgaacatg cccggttact tgaacgctgt gagggtaaac aactggcggt atggatgcgg
7441 cgggtctgcc tgggggggcc ggktgcccgt tccggaaaac tgccgacack ggcaccgccg
7501 ttactgcgtc agctggccgc catcggaaat aacctgaatc agacagcccg taaggtgaac
7561 agcgggcagt ggtcttccgg tgaccgggtt caggtggtgg ccgcactgat ggccatcggg
7621 gatgagctgc gccggctgcg tctggctgtc agggaacagg gggcgcggga tgatagttaa
7681 atttcatgcc aggggaaaag gtggtggcag tggtccggtt gattacctgc tggggaggga
7741 gcgtaaccgc gaaggcgcaa cggtgcttca gggtaatccg gaagaagtcc gggaactcat
7801 cgatgccacg ccatttgcga agaaatacac gtccggtgtt ctgtcgttcg cggagaagga
7861 gctgccgccg ggaggacgtg aaaaagtgat ggcgagcttt gagcgtgtac tgatgcccgg
7921 tctcgaaaaa aatcagtaca gcatcctgtg ggtggagcac caggacaagg gacggcttga
7981 gctgaatttt gtcattccga acatggagct acagaccgga aaacgcctcc agccgtacta
8041 cgaccgcgca gacaggccta gaattgatgc ctggcagacg ctggtaaatc accattacgg
8101 gctgcatgac ccgaacgccc cggagaaccg caggacgctg acactccctg ataacctgcc
8161 tgaaacgaaa caggcgctat ttcttccaga attgccatga ttttttcccc acgggaggcg
8221 tcactggctc ccgtgttgtc ggcagctttg attcgataag cagcatcgcc tgtttcaggc
8281 tgtctatgtg tgactgttga gctgtaacaa gttgtctcag gtgttcaatt tcatgttcta
8341 gttgctttgt tttactggtt tcacctgttc tattaggtgt tacatgctgt tcatctgtta
8401 cattgtcgat ctgttcatgg tgaacagctt taaatgcacc aaaaactcgt aaaagctctg
8461 atgtatctat cttttttaca ccgttttcat ctgtgcatat ggacagtttt ccctttgata
8521 tgtaacggtg aacagttgtt ctacttttgt ttgttagtct tgatgcttca ctgatagata
8581 caagagccat aagaacctca gatccttccg tatttagcca gtatgttctc tagtgtggtt
8641 cgttgttttt gcgtgagcca tgagaacgaa ccattgagat catacttact ttgcatgtca
8701 ctcaaaaatt ttgcctcaaa actggtgagc tgaatttttg cagttaaagc atcgtgtagt
8761 gtttttctta gtccgttatg taggtaggaa tctgatgtaa tggttgttgg tattttgtca
8821 ccattcattt ttatctggtt gttctcaagt tcggttacga gatccatttg tctatctagt
8881 tcaacttgga aaatcaacgt atcagtcggg cggcctcgct tatcaaccac caatttcata
8941 ttgctgtaag tgtttaaatc tttacttatt ggtttcaaaa cccattggtt aagcctttta
9001 aactcatggt agttattttc aagcattaac atgaacttaa attcatcaag gctaatctct
9061 atatttgcct tgtgagtttt cttttgtgtt agttctttta ataaccactc ataaatcctc
9121 atagagtatt tgttttcaaa agacttaaca tgttccagat tatattttat gaattttttt
9181 aactggaaaa gataaggcaa tatctcttca ctaaaaacta attctaattt ttcgcttgag
9241 aacttggcat agtttgtcca ctggaaaatc tcaaagcctt taaccaaagg attcctgatt
9301 tccacagttc tcgtcatcag ctctctggtt gctttagcta atacaccata agcattttcc
9361 ctactgatgt tcatcatctg aacgtattgg ttataagtga acgataccgt ccgttctttc
9421 cttgtagggt tttcaatcgt ggggttgagt agtgccacac agcataaaat tagcttggtt
9481 tcatgctccg ttaagtcata gcgactaatc gctagttcat ttgctttgaa aacaactaat
9541 tcagacatac atctcaattg gtctaggtga ttttaatcac tataccaatt gagatgggct
9601 agtcaatgat aattactagt ccttttcctt tgagttgtgg gtatctgtaa attctgctag
9661 acctttgctg gaaaacttgt aaattctgct agaccctctg taaattccgc tagacctttg
9721 tgtgtttttt ttgtttatat tcaagtggtt ataatttata gaataaagaa agaataaaaa
9781 aagataaaaa gaatagatcc cagccctgtg tataactcac tactttagtc agttccgcag
9841 tattacaaaa ggatgtcgca aacgctgttt gctcctctac aaaacagacc ttaaaaccct
9901 aaaggcttaa gtagcaccct cgcaagctcg ggcaaatcgc tgaatattcc ttttgtctcc
9961 gaccatcagg cacctgagtc gctgtctttt tcgtgacatt cagttcgctg cgctcacggc
10021 tctggcagtg aatgggggta aatggcacta caggcgccca acagtccccc ggccacgggg
10081 cctgccacca tacccacgcc gaaacaagcg ctgatgtccg gcggtgcttt tgccgttacg
10141 caccaccccg tcagtagctg aacaggaggg acagctgata gaaacagaag ccactggagc
10201 acctcaaaaa caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt
10261 gcgccgaata aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc
10321 ctgttgatac cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg
10381 taagaggttc caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt
10441 tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
10501 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
10561 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
10621 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
10681 atccggaatt c
//
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