| 质粒名称: | pYK225 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000805 |
| 产品用途: | Mammalian PhCMV-driven constitutive MCP-mCherry-2xANR expression vector (PhCMV-MCP-mCherry-2xANR-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | MCP-mCherry-2xANR |
| 物种来源: |
synthetic
|
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK225
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK225载体质粒基本信息
pYK225质粒图谱载体图谱和pYK225载体序列质粒序列多克隆位点信息
pYK225质粒载体简介
pYK225质粒序列载体序列
LOCUS pYK225 6835 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6835)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 27, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6835
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 881..952
/label=oYK57-NLS-F
primer_bind 881..901
/label=oYK31-mCherry-NS3a-F
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
primer_bind 886..934
/label=oSCL1118
primer_bind 897..943
/label=oYK05-mcp-F
primer_bind 911..934
/label=Oscl32-1
primer_bind 911..934
/label=Oscl42-1
regulatory 918..926
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
primer_bind 923..949
/label=oYK235_MCP-F
primer_bind 923..948
/label=oYK77_mcpns3a_F
CDS 926..1312
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
VKAMQGLLKDGNPIPSAIAANSGIY"
primer_bind 926..952
/label=oYK57-mcp-mCherry-F
primer_bind 926..952
/label=oYK228_mcp_F
primer_bind complement(1292..1342)
/label=oSCL093
primer_bind complement(1292..1312)
/label=Oscl32-2
primer_bind complement(1312..1338)
/label=oDJ171-4
primer_bind 1312..1332
/label=oLZ048 NSP3-F
primer_bind 1313..1372
/label=oDJ171-3
misc_feature 1313..1348
/label=linker
misc_feature 1313..1347
/label=linker
primer_bind 1313..1342
/label=oSCL388
primer_bind 1313..1332
/label=oSCL1192
primer_bind 1313..1330
/label=oLZ147 NS3a F
primer_bind 1313..1329
/label=oYF0033
primer_bind 1313..1328
/label=oYK28-mCherry-NS3a-F
primer_bind complement(1323..1360)
/label=oSCL1119
primer_bind complement(1323..1342)
/label=oSCL075
primer_bind complement(1331..1358)
/label=oYK05-mcp-R
primer_bind complement(1331..1349)
/label=oYK82_mcp_R
primer_bind 1333..1367
/label=oYK03-02-F
primer_bind 1339..1366
/label=oYK05-mcherry-F
primer_bind 1343..1375
/label=omz_linker_mCherry_F
CDS 1349..2053
/codon_start=1
/label=mCherry
/translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
GRHSTGGMDELYK"
primer_bind 1349..1369
/label=oXQ135
primer_bind 1349..1369
/label=oXQ174
primer_bind 1349..1369
/label=omz_mCherry_F
primer_bind 1349..1366
/label=oYK31-mCherry-NS3a-F
misc_feature 1349..1351
/label=Coh2
primer_bind complement(1661..1672)
/label=oSCL011
primer_bind complement(1661..1672)
/label=omz_Coh2_R
primer_bind complement(1661..1672)
/label=omz_Coh2_His_R
primer_bind complement(2032..2073)
/label=oYK83_mcp_mcherry_R
primer_bind complement(2033..2086)
/label=oDJ171-4
primer_bind complement(2033..2055)
/label=oXQ136
primer_bind complement(2033..2053)
/label=oXQ161
primer_bind complement(2033..2053)
/label=omz_mCherry_His_R
primer_bind complement(2033..2053)
/label=oYK05-mcherry-R
primer_bind complement(2035..2073)
/label=omz_mCherry_linker_R
CDS 2054..2128
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(2054..2073)
/label=oYK82_mcp_R
primer_bind complement(2054..2073)
/label=oYK195_ANR_R
primer_bind 2054..2068
/label=oYK82_LinkANR_F
primer_bind complement(2114..2128)
/label=oYK89_mCherry_R
CDS 2129..2191
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 2129..2144
/label=Primer 4
primer_bind complement(2168..2211)
/label=oYK195_ANR_R
primer_bind complement(2173..2191)
/label=oYK82_LinkANR_R
primer_bind 2176..2190
/label=Primer 3
CDS 2192..2266
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(2192..2211)
/label=oYK82_mcp_R
primer_bind complement(2192..2211)
/label=oYK83_mcp_mcherry_R
primer_bind 2192..2206
/label=oYK82_LinkANR_F
primer_bind complement(2252..2266)
/label=oYK89_mCherry_R
CDS 2267..2329
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 2267..2282
/label=Primer 4
primer_bind complement(2306..2329)
/label=oYK195_ANR_R
primer_bind complement(2311..2352)
/label=oYK82_LinkANR_R
primer_bind 2314..2328
/label=Primer 3
primer_bind complement(2330..2352)
/label=oYK05-mcherry-R
primer_bind complement(2333..2355)
/label=oYK57-MCP-mCherry-R
primer_bind complement(2356..2373)
/label=oXQ161
terminator 2429..2656
/label=bGH PA
primer_bind complement(2660..2679)
/label=oYK246_BGH-R
misc_feature 2702..3130
/label=f1 origin
misc_feature 3135..3478
/label=SV40 early promoter and origin
misc_feature 3324..3401
/label=SV40 ORI
CDS 3543..4334
/label=Kan/neoR
misc_feature 4508..4638
/label=SV40 early polyadenylation signal
primer_bind complement(4674..4694)
/label=M13-rev
misc_binding complement(4700..4722)
/label=LacO
promoter complement(4727..4756)
/label=lac
misc_feature complement(5021..5694)
/label=pUC origin
CDS complement(5842..6501)
/label=AmpR
promoter complement(6741..6769)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttgcca ccatggcttc aaactttact cagttcgtgc tcgtggacaa
961 tggtgggaca ggggatgtga cagtggctcc ttctaatttc gctaatgggg tggcagagtg
1021 gatcagctcc aactcacgga gccaggccta caaggtgaca tgcagcgtca ggcagtctag
1081 tgcccagaag agaaagtata ccatcaaggt ggaggtcccc aaagtggcta cccagacagt
1141 gggcggagtc gaactgcctg tcgccgcttg gaggtcctac ctgaacatgg agctcactat
1201 cccaattttc gctaccaatt ctgactgtga actcatcgtg aaggcaatgc aggggctcct
1261 caaagacggt aatcctatcc cttccgccat cgccgctaac tcaggtatct acgccagcgg
1321 aagtggcgga ggaggcgacg tcggatccgt gagcaagggc gaggaggata acatggccat
1381 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
1441 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
1501 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
1561 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
1621 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
1681 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
1741 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
1801 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
1861 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
1921 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
1981 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
2041 cgagctgtac aagggctccg gaacgggcag cgggaccgga agtggcaccg gaaccacttc
2101 cggaaccggt actggcggga gtaccggcgg tgagctagac gaactcgtgt acctgttgga
2161 tggccccggt tacgacccaa tccacagcga tggctccgga acgggcagcg ggaccggaag
2221 tggcaccgga accacttccg gaaccggtac tggcgggagt accggcggtg agctagacga
2281 actcgtgtac ctgttggatg gccccggtta cgacccaatc cacagcgatt aaggatccac
2341 tagtccagtg tggtggaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga
2401 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
2461 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
2521 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
2581 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
2641 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
2701 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
2761 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
2821 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
2881 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
2941 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
3001 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
3061 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
3121 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
3181 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
3241 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
3301 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
3361 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
3421 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
3481 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca
3541 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
3601 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
3661 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
3721 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
3781 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
3841 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
3901 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
3961 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
4021 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
4081 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
4141 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
4201 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
4261 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
4321 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
4381 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
4441 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
4501 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
4561 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
4621 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
4681 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
4741 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
4801 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
4861 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
4921 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
4981 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
5041 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
5101 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
5161 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
5221 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
5281 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
5341 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
5401 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
5461 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
5521 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
5581 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
5641 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
5701 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
5761 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
5821 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
5881 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
5941 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
6001 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
6061 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
6121 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
6181 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
6241 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
6301 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
6361 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
6421 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
6481 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
6541 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
6601 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
6661 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
6721 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
6781 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
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