| 质粒名称: | Circular-ic-CVB3-GFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 226260 |
| 存储实验室: | Depositing Lab |
|
载体骨架基本信息 |
|
| 载体名称: | 未知 |
| 载体出品公司: | Clontech |
| 空载体大小: | 6580 bp |
| 完整质粒大小: | 6580 bp |
| 载体类型: | In Vitro Transcription |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | EGFP |
| 物种来源: | Synthetic |
| 克隆方法: | Gibson Cloning |
|
文献引用方法 |
|
| 材料和方法部分: | Circular-ic-CVB3-GFP was a gift from Prashant Mali (Addgene plasmid # 226260 ; http://n2t.net/addgene:226260 ; RRID:Addgene_226260) |
| 参考文献部分: | Robust genome and cell engineering via in vitro and in situ circularized RNAs. Tong M, Palmer N, Dailamy A, Kumar A, Khaliq H, Han S, Finburgh E, Wing M, Hong C, Xiang Y, Miyasaki K, Portell A, Rainaldi J, Suhardjo A, Nourreddine S, Chew WL, Kwon EJ, Mali P. Nat Biomed Eng. 2024 Aug 26. doi: 10.1038/s41551-024-01245-z. 10.1038/s41551-024-01245-z PubMed 39187662 |
买家导航
Circular-ic-CVB3-GFP载体质粒基本信息
Circular-ic-CVB3-GFP质粒图谱载体图谱和Circular-ic-CVB3-GFP载体序列质粒序列多克隆位点信息
Circular-ic-CVB3-GFP质粒载体简介
Circular-ic-CVB3-GFP质粒序列载体序列
LOCUS Circular-ic-CVB3-GFP 5632 bp DNA circular SYN 14-APR-2025
DEFINITION Production of in situ circularizable RNA via IVT.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5632)
AUTHORS Tong M, Palmer N, Dailamy A, Kumar A, Khaliq H, Han S, Finburgh E,
Wing M, Hong C, Xiang Y, Miyasaki K, Portell A, Rainaldi J, Suhardjo
A, Nourreddine S, Chew WL, Kwon EJ, Mali P
TITLE Robust genome and cell engineering via in vitro and in situ
circularized RNAs.
JOURNAL Nat Biomed Eng. 2024 Aug 26. doi: 10.1038/s41551-024-01245-z.
PUBMED 39187662
REFERENCE 2 (bases 1 to 5632)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat Biomed
Eng. 2024 Aug 26. doi: 10.1038/s41551-024-01245-z."
COMMENT Sequence Label: Circular-ic-CVB3-GFP
FEATURES Location/Qualifiers
source 1..5632
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 12..141
/label=AAV2 ITR
primer_bind 226..245
/label=T7
/note="T7 promoter, forward primer"
promoter 226..244
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS join(1094..1096,1097..1099,1100..1813)
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS join(1094..1096,1097..1099,1100..1810)
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(1139..1160)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1400..1419)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1747..1768
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(1873..1893)
/label=WPRE-R
/note="WPRE, reverse primer"
repeat_region 2693..2822
/label=AAV2 ITR
primer_bind complement(2841..2858)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(2841..2857)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2850..2872)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 2999..3454
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3086..3105)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3296..3317
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 3480..3584
/gene="bla"
/label=AmpR promoter
CDS join(3585..3653,3654..4445)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(3803..3822)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 4616..5204
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5105..5124
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
protein_bind 5492..5513
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter join(5528..5533,5534..5551,5552..5558)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5566..5582
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5571..5593
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 5590..5606
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 5590..5606
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
ORIGIN
1 ccttaattag gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt
181 agccatgctc taggaagatc ggaattcgcc cttaagctag gatgctaata cgactcacta
241 tagggccatc agtcgccggt cccaagcccg gataaaatgg gagggggcgg gaaaccgcct
301 aaccatgccg actgatggca gagcggccgc gtcgacgggc ccgcggaatt ccttaaaaca
361 gcctgtgggt tgatcccacc cacaggccca ttgggcgcta gcactctggt atcacggtac
421 ctttgtgcgc ctgttttata ccccctcccc caactgtaac ttagaagtaa cacacaccga
481 tcaacagtca gcgtggcaca ccagccacgt tttgatcaag cacttctgtt accccggact
541 gagtatcaat agactgctca cgcggttgaa ggagaaagcg ttcgttatcc ggccaactac
601 ttcgaaaaac ctagtaacac cgtggaagtt gcagagtgtt tcgctcagca ctaccccagt
661 gtagatcagg tcgatgagtc accgcattcc ccacgggcga ccgtggcggt ggctgcgttg
721 gcggcctgcc catggggaaa cccatgggac gctctaatac agacatggtg cgaagagtct
781 attgagctag ttggtagtcc tccggcccct gaatgcggct aatcctaact gcggagcaca
841 caccctcaag ccagagggca gtgtgtcgta acgggcaact ctgcagcgga accgactact
901 ttgggtgtcc gtgtttcatt ttattcctat actggctgct tatggtgaca attgagagat
961 cgttaccata tagctattgg attggccatc cggtgactaa tagagctatt atatatccct
1021 ttgttgggtt tataccactt agcttgaaag aggttaaaac attacaattc attgttaagt
1081 tgaatacagc aaaatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
1141 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
1201 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
1261 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
1321 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
1381 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
1441 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
1501 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
1561 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
1621 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
1681 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
1741 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
1801 gctgtacaag taaggatcca atcaacctct ggattacaaa atttgtgaaa gattgactgg
1861 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
1921 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
1981 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
2041 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
2101 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
2161 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
2221 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
2281 ctacaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
2341 tcttcgaagt aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
2401 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
2461 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaactg
2521 ccatcagtcg gcgtggactg tagaacactg ccaatgccgg tcccaagccc ggataaaagt
2581 ggagggtaca gtccacgctt tttttctcga gttaagggcg aattcccgat aaggatcttc
2641 ctagagcatg gctacgtaga taagtagcat ggcgggttaa tcattaacta caaggaaccc
2701 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
2761 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
2821 agccttaatt aacctaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
2881 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
2941 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
3001 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
3061 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
3121 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
3181 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
3241 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
3301 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
3361 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
3421 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
3481 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
3541 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
3601 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
3661 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
3721 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
3781 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
3841 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
3901 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
3961 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
4021 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
4081 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
4141 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
4201 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
4261 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
4321 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
4381 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
4441 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
4501 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
4561 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
4621 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
4681 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
4741 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
4801 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
4861 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
4921 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
4981 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
5041 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
5101 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
5161 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
5221 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
5281 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
5341 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
5401 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
5461 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
5521 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
5581 aatttcacac aggaaacagc tatgaccatg attacgccag atttaattaa gg
//
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