pAAV-CAG-KCNMA1-EGFP

LOCUS       pAAV-CAG-KCNMA1-EGFP               9154 bp DNA     circular SYN 16-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CAG-KCNMA1-EGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 17, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        279..556
                     /label=chicken beta-actin promoter
     primer_bind     955..996
                     /label=KCNMA-F1
     misc_feature    977..4690
                     /label=KCNMA1-3
     regulatory      977..986
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4667..4690)
                     /label=KCNMA-R1
     primer_bind     4672..4711
                     /label=2418-F1
     primer_bind     4673..4714
                     /label=2418-F2
     CDS             4697..5409
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     primer_bind     complement(5391..5430)
                     /label=2418-R2
     primer_bind     5392..5410
                     /label=Kv2.1-Fw-BsrGI
     primer_bind     complement(5401..5432)
                     /label=2418-R1
     misc_feature    5438..6026
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5909..5920)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6069..6190
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6531..6540)
                     /label=PA-F6A-F1
     rep_origin      complement(6718..7306)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7477..8337)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8338..8442)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8468..8923)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
       61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
      301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
      361 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
      421 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
      481 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
      541 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
      601 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
      661 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
      721 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
      781 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
      841 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
      901 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
      961 aattggatcc ggtaccgcca ccatggcaaa cggtggtggt ggaggtggag gtagtagcgg
     1021 cggtggaggc ggtggcggtg gtagctcact ccggatgagc agcaacatcc acgcaaacca
     1081 cctgtcactg gatgcttcca gcagctccag cagtagctct agcagctcta gttcttctag
     1141 cagcagctca tctagcagta gcgtgcacga gcctaagatg gacgcactga tcatacctgt
     1201 gactatggag gtgccatgtg attctagggg ccaaagaatg tggtgggctt tcttggcctc
     1261 cagcatggta accttcttcg gcggtttgtt catcatactg ctgtggagaa ctctgaagta
     1321 cctgtggacc gtgtgttgtc actgtggcgg taagaccaaa gaagctcaga agatcaacaa
     1381 cggctctagc caagctgacg gtacattgaa gccagtcgat gagaaagaag aagctgttgc
     1441 tgcagaggtt ggctggatga cctccgtgaa agactgggca ggcgtcatga tctccgctca
     1501 gacactgact ggtagggtac tggtcgtgct ggtgtttgct ctctctatcg gtgcactggt
     1561 gatctacttc atcgatagta gcaatccaat cgagagttgt cagaacttct acaaagactt
     1621 cacactccaa atcgacatgg ctttcaacgt gttctttctc ttgtacttcg gtctgagatt
     1681 tatcgccgcc aatgacaaac tctggttctg gcttgaggtc aacagtgtgg tggacttctt
     1741 cacagtgcca ccagtgttcg tgtctgtcta cctgaacaga agctggctgg gtctgaggtt
     1801 tctgcgggca cttcggctga tccagttctc cgagattctc cagtttctga acatacttaa
     1861 gactagcaac tccattaagc tggtcaacct gctcagcatc ttcatcagca cctggctgac
     1921 tgctgctggc ttcatccatc tggtcgagaa cagcggcgac ccttgggaga acttccagaa
     1981 caaccaggct ctcacctact gggaatgcgt gtaccttctg atggtgacca tgtccaccgt
     2041 tggctatgga gacgtgtatg ccaagaccac tcttggcagg ctgtttatgg tgttcttcat
     2101 tctgggtgga ttggccatgt tcgctagtta tgtgcctgag atcatcgagc tgatcggcaa
     2161 tcgcaagaag tatggcggtt cttactccgc tgtgagcgga agaaagcaca tcgttgtgtg
     2221 cggccacatc actctggaaa gtgtctctaa cttcttgaaa gactttctgc acaaagacag
     2281 ggatgacgtc aacgtggaaa tcgtgttctt gcacaacatc tcccctaacc tggagttgga
     2341 agctctgttt aagcggcatt tcacacaggt ggagttctat cagggtagtg tgctcaatcc
     2401 acacgatctt gctagggtca agatcgagag cgccgatgcc tgtctgattc tcgccaacaa
     2461 gtactgtgcc gatccagatg ctgaagatgc cagcaacatt atgcgcgtca tttccatcaa
     2521 gaactaccac cctaagatcc gcatcatcac tcagatgctg cagtaccaca acaaggctca
     2581 tctgctgaac attccatctt ggaattggaa agaaggtgat gacgccatct gtctcgcaga
     2641 gctgaagctg ggtttcatcg cacagagctg tttggcacag ggactgtcta ccatgcttgc
     2701 caacttgttc agcatgcgga gcttcatcaa gattgaagaa gatacttggc agaagtacta
     2761 cctggagggt gtgtccaacg agatgtacac cgagtatctg agttccgcct tcgttggttt
     2821 gtctttccca actgtgtgtg aactgtgctt tgtgaagctc aagctgctca tgattgccat
     2881 cgagtacaag tccgctaaca gggaaagccg aatactgatc aatccaggta accacctcaa
     2941 gattcaagag ggaacgcttg gtttctttat cgcctctgat gccaaagagg tgaagagggc
     3001 tttcttctac tgcaaggcct gccacgatga cattacagac cctaagagaa tcaagaagtg
     3061 tggctgtaag cggccaaaga tgagcatcta taagcgcatg cgaagagcct gttgctttga
     3121 ttgcggcaga tctgagagag attgcagctg tatgtcaggt agggtgagag gtaacgtgga
     3181 cactcttgaa cgagcctttc cattgagctc cgtgtccgtc aacgactgtt ccaccagttt
     3241 ccgggccttt gaggacgaac agccaagtac tctgtctcca aagaagaaac agcggaatgg
     3301 cggtatgcgg aactctccaa acacctcccc taaactcatg cgccacgatc cattgctgat
     3361 ccctggtaac gaccagatcg acaacatgga ctcaaacgtc aagaaatacg actcaaccgg
     3421 catgttccac tggtgcgctc caaaggagat cgagaaggtc attctgacaa gaagcgaggc
     3481 agcaatgacc gtgctttccg gtcatgtcgt cgtgtgcatc tttggtgacg tctctagcgc
     3541 tctgatcggc ctgagaaatc tggtgatgcc tctcagagct tctaacttcc attaccatga
     3601 gctcaagcat atcgtgttcg tcggttccat agaatatctc aagcgggagt gggaaacctt
     3661 gcacaacttc ccaaaggtca gcattctgcc aggtacacct ctgagtaggg ctgaccttcg
     3721 ggctgtgaac atcaacttgt gcgatatgtg tgtcattctc tctgccaatc agaacaatat
     3781 cgacgacact agcttgcaag acaaagagtg catcttggct tctctgaaca ttaagagcat
     3841 gcaattcgac gattccattg gtgttctgca ggccaactct cagggattca ctccacctgg
     3901 tatggacaga tccagccctg ataattcccc tgtgcatggc atgttgcggc aaccttccat
     3961 cacaactggc gtaaacattc caatcatcac agagctggtg aacgacacca acgtgcagtt
     4021 tctggaccaa gatgacgatg acgatccaga cactgagctg tacctgacac agccatttgc
     4081 ctgtggtaca gccttcgctg tctccgttct ggatagcctc atgagcgcta cctacttcaa
     4141 cgacaacata ctgacactta tccggacatt ggtgacaggc ggagctactc cagagcttga
     4201 agcactgatc gctgaagaga acgcactgag aggcggctac tctacaccac agactttggc
     4261 aaacagggat cggtgtaggg tagctcagct ggctttgctg gacggtccat ttgccgattt
     4321 gggtgacggt ggctgctatg gtgacctgtt ctgtaaggct ctgaaaacct acaatatgct
     4381 gtgcttcggc atctataggc tgcgcgatgc acatctgtct accccatctc agtgcaccaa
     4441 gagatatgtg atcactaatc caccttacga gttcgaactg gtgccaaccg atctgatctt
     4501 ctgtctgatg cagttcgatc ataacgctgg tcagagcaga gcaagcctct ctcattcttc
     4561 tcactccagc cagtccagta gcaagaaatc atccagcgtg cacagcatcc caagcaccgc
     4621 taatcgccag aacaggccta agagccgaga gtctagggac aaacagaagt acgttcaaga
     4681 agagaggctc ggctctgtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     4741 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     4801 cgatgccacc tacggcaagc tgaccctgaa gttcatttgc accaccggca agctgcccgt
     4861 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     4921 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     4981 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     5041 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     5101 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     5161 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     5221 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     5281 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     5341 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     5401 gctgtacaag taggaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     5461 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5521 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     5581 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     5641 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     5701 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     5761 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     5821 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     5881 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     5941 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     6001 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cccgggcggc cgcttcgagc
     6061 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     6121 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     6181 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
     6241 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatcttcc
     6301 tagagcatgg ctacgtagat aagtagcatg gcgggttaat cattaactac aaggaacccc
     6361 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     6421 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     6481 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6541 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6601 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6661 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6721 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6781 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     6841 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     6901 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6961 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7021 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7081 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7141 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7201 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7261 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7321 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7381 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7441 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7501 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7561 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7621 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7681 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7741 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     7801 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     7861 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     7921 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     7981 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8041 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8101 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8161 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8221 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8281 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8341 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8401 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8461 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     8521 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     8581 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     8641 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     8701 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     8761 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     8821 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     8881 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     8941 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctgcgc
     9001 gctcgctcgc tcactgaggc cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc
     9061 ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat cactaggggt
     9121 tccttgtagt taatgattaa cccgccatgc tact
//