| 质粒名称: | pAAVLINK-NOTCH1-B |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001764 |
| 产品用途: | Body' AAVLINK plasmid for NOTCH1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | NOTCH1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-NOTCH1-B
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-NOTCH1-B载体质粒基本信息
pAAVLINK-NOTCH1-B质粒图谱载体图谱和pAAVLINK-NOTCH1-B载体序列质粒序列多克隆位点信息
pAAVLINK-NOTCH1-B质粒载体简介
pAAVLINK-NOTCH1-B质粒序列载体序列
LOCUS pAAVLINK-NOTCH1-B 6239 bp DNA circular SYN 24-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6239)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6239
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
protein_bind 145..178
/label=loxJTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 179..270
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 271..3395
/codon_start=1
/label=NOTCH1
/translation="VPRVRWCWPRVPPAPAETAGSAGNPRTMRASPVSAPRAGKGRPVR
STSTSAF*ARAGTAHPARTPTAATAATARPATVGATARPTSTTAGPTRVTTGAPAQTAS
TRPSATACPASGALSVRRTSTSVPVTPAATGPTARTAWTATRAPAPQASAGSTVRTTRL
TAQRAPASTVAPAWTASTRSPACVHPASRAATASTMSMSATHSPACMAAPVRTAAAPTG
APAPRATLAPTARTLCTGVTPRPARTAANAGRPTPSTAASAPAAGPAFTATCPACPVRW
LRSDKVLTLPACASMEGSVWTRATRTTAAARRATQAATVRTWWTSAHPAPARTGPPART
TWAATPASAWPATTG*TALRRSTSASPTPARTGAPASTSPTPTSAPAHGALRVCTVRST
WTTAIPPLTPCPGAPSALTTAPAWTRWAATAAPARRASWVSAVRGMSTSACPIPATPVA
PRTACSASMTSTASAVLVTPGAAASPSSMAAKASPARMGAPAPWPPTPPAGSSASALRA
SRAPRVRMTLVPAAACAASTAAHASPARAAPPACAWAPSRAPNASSRPAAPAWAATPAT
TRGPVSPHPRAPSTVACAPPNSTGSCATSWTTASGVGPGATSPRR*SRRRASCPSARRT
RATRSAACSATTTRAAGTAVTAPSTSMTPGRTARSLCSAGSTSVTATVTASATQPAASS
TALTASVRKASATPCTTSTARTTSATGTATRAATARSASGTGWTVRSMYPRGWRPARWW
WWC*CRRSSCATAPSTSCGSSAACCTPTWSSSVTHTASR*SSPTTAARRSCASTPSSVP
PRAGPHLTPCWAR*RPRCSLVAARVGGGGGSWTPWTSAAPSSTWRLTTGSVCRPPRSAS
RVPPTWPHSWERSPRWAASTSPTRSRPCRVRPWSRPRRRSCTSCTWRRPPLCFCSSWAA
GCCCPASAGGSMASSGSLRASKCLRPARRSGGSPSARTPWASSP*RTLQTVPSWTTTRM
SGGTRTWRPRSSGSRSPWFCLTWTTRQTTGSGLSSTWMPLTCACLPWPPHRP"
misc_feature 631..636
/label=SDS
misc_feature 2140..2145
/label=SDS
misc_feature 2167..2172
/label=SDS
misc_feature 2671..2676
/label=SDS
misc_feature 2866..2871
/label=SDS
intron 3396..3436
/label=Splicing Donor
misc_feature 3440..3473
/label=lox2272-JT1571
repeat_region 3502..3642
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3502..3631
/label=AAV2 ITR
rep_origin 3717..4172
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4454..4558
/gene="bla"
/label=AmpR promoter
CDS 4559..5419
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5590..6178
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtataact tcgtatagca tacattatag caatttatga
181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
241 ctgacatcca ctttgccttt ctctccacag gtgccacgtg tgaggtggtg ctggccccgt
301 gtgcccccag cccctgcaga aacggcgggg agtgcaggca atccgaggac tatgagagct
361 tctcctgtgt ctgccccacg ggctggcaag ggcagacctg tgaggtcgac atcaacgagt
421 gcgttctgag cccgtgccgg cacggcgcat cctgccagaa cacccacggc ggctaccgct
481 gccactgcca ggccggctac agtgggcgca actgcgagac cgacatcgac gactgccggc
541 ccaacccgtg tcacaacggg ggctcctgca cagacggcat caacacggcc ttctgcgact
601 gcctgcccgg cttccggggc actttctgtg aggaggacat caacgagtgt gccagtgacc
661 cctgccgcaa cggggccaac tgcacggact gcgtggacag ctacacgtgc acctgccccg
721 caggcttcag cgggatccac tgtgagaaca acacgcctga ctgcacagag agctcctgct
781 tcaacggtgg cacctgcgtg gacggcatca actcgttcac ctgcctgtgt ccacccggct
841 tcacgggcag ctactgccag cacgatgtca atgagtgcga ctcacagccc tgcctgcatg
901 gcggcacctg tcaggacggc tgcggctcct acaggtgcac ctgcccccag ggctacactg
961 gccccaactg ccagaacctt gtgcactggt gtgactcctc gccctgcaag aacggcggca
1021 aatgctggca gacccacacc cagtaccgct gcgagtgccc cagcggctgg accggccttt
1081 actgcgacgt gcccagcgtg tcctgtgagg tggctgcgca gcgacaaggt gttgacgttg
1141 cccgcctgtg ccagcatgga gggctctgtg tggacgcggg caacacgcac cactgccgct
1201 gccaggcggg ctacacaggc agctactgtg aggacctggt ggacgagtgc tcacccagcc
1261 cctgccagaa cggggccacc tgcacggact acctgggcgg ctactcctgc aagtgcgtgg
1321 ccggctacca cggggtgaac tgctctgagg agatcgacga gtgcctctcc cacccctgcc
1381 agaacggggg cacctgcctc gacctcccca acacctacaa gtgctcctgc ccacggggca
1441 ctcagggtgt gcactgtgag atcaacgtgg acgactgcaa tccccccgtt gaccccgtgt
1501 cccggagccc caagtgcttt aacaacggca cctgcgtgga ccaggtgggc ggctacagct
1561 gcacctgccc gccgggcttc gtgggtgagc gctgtgaggg ggatgtcaac gagtgcctgt
1621 ccaatccctg cgacgcccgt ggcacccaga actgcgtgca gcgcgtcaat gacttccact
1681 gcgagtgccg tgctggtcac accgggcgcc gctgcgagtc cgtcatcaat ggctgcaaag
1741 gcaagccctg caagaatggg ggcacctgcg ccgtggcctc caacaccgcc cgcgggttca
1801 tctgcaagtg ccctgcgggc ttcgagggcg ccacgtgtga gaatgacgct cgtacctgcg
1861 gcagcctgcg ctgcctcaac ggcggcacat gcatctccgg cccgcgcagc cccacctgcc
1921 tgtgcctggg ccccttcacg ggccccgaat gccagttccc ggccagcagc ccctgcctgg
1981 gcggcaaccc ctgctacaac caggggacct gtgagcccac atccgagagc cccttctacc
2041 gttgcctgtg ccccgccaaa ttcaacgggc tcttgtgcca catcctggac tacagcttcg
2101 ggggtggggc cgggcgcgac atccccccgc cgctgatcga ggaggcgtgc gagctgcccg
2161 agtgccagga ggacgcgggc aacaaggtct gcagcctgca gtgcaacaac cacgcgtgcg
2221 gctgggacgg cggtgactgc tccctcaact tcaatgaccc ctggaagaac tgcacgcagt
2281 ctctgcagtg ctggaagtac ttcagtgacg gccactgtga cagccagtgc aactcagccg
2341 gctgcctctt cgacggcttt gactgccagc gtgcggaagg ccagtgcaac cccctgtacg
2401 accagtactg caaggaccac ttcagcgacg ggcactgcga ccagggctgc aacagcgcgg
2461 agtgcgagtg ggacgggctg gactgtgcgg agcatgtacc cgagaggctg gcggccggca
2521 cgctggtggt ggtggtgctg atgccgccgg agcagctgcg caacagctcc ttccacttcc
2581 tgcgggagct cagccgcgtg ctgcacacca acgtggtctt caagcgtgac gcacacggcc
2641 agcagatgat cttcccctac tacggccgcg aggaggagct gcgcaagcac cccatcaagc
2701 gtgccgccga gggctgggcc gcacctgacg ccctgctggg ccaggtgaag gcctcgctgc
2761 tccctggtgg cagcgagggt gggcggcggc ggagggagct ggaccccatg gacgtccgcg
2821 gctccatcgt ctacctggag attgacaacc ggcagtgtgt gcaggcctcc tcgcagtgct
2881 tccagagtgc caccgacgtg gccgcattcc tgggagcgct cgcctcgctg ggcagcctca
2941 acatccccta caagatcgag gccgtgcaga gtgagaccgt ggagccgccc ccgccggcgc
3001 agctgcactt catgtacgtg gcggcggccg cctttgtgct tctgttcttc gtgggctgcg
3061 gggtgctgct gtcccgcaag cgccggcggc agcatggcca gctctggttc cctgagggct
3121 tcaaagtgtc tgaggccagc aagaagaagc ggcgggagcc cctcggcgag gactccgtgg
3181 gcctcaagcc cctgaagaac gcttcagacg gtgccctcat ggacgacaac cagaatgagt
3241 ggggggacga ggacctggag accaagaagt tccggttcga ggagcccgtg gttctgcctg
3301 acctggacga ccagacagac caccggcagt ggactcagca gcacctggat gccgctgacc
3361 tgcgcatgtc tgccatggcc cccacaccgc cccaggtaag tatcaaggtt acaagacagg
3421 tttaaggaga ccaataagct attattcgta taggatactt tatacgaagt tatgaattcc
3481 acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
3541 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
3601 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt
3661 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
3721 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
3781 acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
3841 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
3901 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
3961 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
4021 cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg
4081 gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc
4141 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
4201 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
4261 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
4321 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
4381 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
4441 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
4501 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
4561 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
4621 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
4681 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
4741 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
4801 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
4861 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
4921 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
4981 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
5041 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
5101 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
5161 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
5221 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
5281 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
5341 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
5401 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
5461 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
5521 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
5581 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
5641 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
5701 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
5761 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
5821 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
5881 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
5941 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
6001 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
6061 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
6121 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
6181 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
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