hOC-GFPtpz

LOCUS       hOC-GFPtpz               10852 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  Expresses GFP in mature bone cells..
ACCESSION   .
VERSION     .
KEYWORDS    hOC-GFPtpz
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10852)
  AUTHORS   Bilic-Curcic I, Kronenberg M, Jiang X, Bellizzi J, Mina M, 
            Marijanovic I, Gardiner EM, Rowe DW
  TITLE     Visualizing levels of osteoblast differentiation by a two-color 
            promoter-GFP strategy: Type I collagen-GFPcyan and 
            osteocalcin-GFPtpz.
  JOURNAL   Genesis. 2005 Oct;43(2):87-98. doi: 10.1002/gene.20156.
  PUBMED    16149065
REFERENCE   2  (bases 1 to 10852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10852
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     38..60
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     52..69
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     53..69
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3998..4717
                     /codon_start=1
                     /product="enhanced yellow variant of GFP"
                     /label=Topaz YFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMRQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4043..4064)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4304..4323)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4651..4672
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4755..4979
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8536..8553)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8707..9295)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8787..8806)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9466..10326)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10089..10108
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10327..10431)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10499..10517
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10555..10577)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10677..10696
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
       61 acggccagtg ccaagctttc tagtagggct caagtggcgg catctctgtc gaactctctc
      121 catccttcat gtcagtatcc aatgtacatc tacttatagc cacttgacag cccccttcct
      181 ggggcaaaca ctcatattcc tgagggatac aggtccccaa atttatggct cctggatctc
      241 cctggtcttc tcttcccaat accagttagc tgtagcctga aggccctgct gcagcttggg
      301 aagggccccg gctgggaggc agtgacaagt ggaaggccgg cagccgctct gctccctact
      361 gtccccactc cctctgctct ccccacatca cagactggcc tgggggactg ggcagcaggc
      421 gcgaaagtgg tggtgacaaa ggcccccccc cccaagcctg gtgccatctt gcagggcaca
      481 gctccgagtt gtgtcttggg cactcccagg gccccctgct gagtcctcag gcaaggcgct
      541 gctccactat cgccactcag tcaccatgag gccatgggtc actcctgtct ctgggtcctg
      601 gcttccaaat tggctagata atgatcccag ggcctcacca gctctgagat tcagtgctat
      661 cctaagagga acaaagcccc ctgctcttaa agccatgccc cacccattcc agctacagac
      721 cctcacgttg tttgacatgg gcaatttcca tgtctgtaac tcaactgaaa gtgtacttaa
      781 ttcaagaaag tttctcgcta gaaagctctg ttgaggtcag aggattgaag ggccactttg
      841 gggacccttg ctgaagatga tgggctctgc tctggggatg cccagcctag gtggtcacag
      901 aacccagctt gctataaata cttgccgaat ggtggggact tcctgatgtg ccttcatcct
      961 aacatgctct gcagggccca tgacttagcc acagactcca tccatattgt cctgggggaa
     1021 accccaggcc tggagtcagg gacagtggag ggcaaggatt tgggttttct tagatcccag
     1081 tttgtctgtt gtctcccatt gatttcaggc tctacacaga gaacagaggg agctggttgc
     1141 tgtgctgagg gagcctgagt gaggagaccg ccagccccag aagcagaggg cagtcaaggt
     1201 caagagcctg tggtccagca tgcctggcct gggcgggcta cctctgagaa cggctgaaat
     1261 ggtgcccagt ccatcagcag tgatggaatt tgctggagga ctaggccaga gcaagcctca
     1321 ctgccactgt gcctttgggg cacccttggg gttggacata cacccccttt agattcctct
     1381 gtttcttcta cctggataat tcttggccat gttctctctt ctctaggttc aggtcagctc
     1441 tgcccctccg cccccctcct gctggttccc cagccctttt ccctggccct ggcttggaga
     1501 atctgttttc aatctccact gattgccccc ttgctggcca gcccaggggc ctttaccatg
     1561 ttctctccac atccgtaaat aaacttcctt cactacactg taatctgtga gcatgcttcc
     1621 tctgccttgg cctctcttgg gccaagacct gctcctgggc tcccaaatgc tggctccagt
     1681 caaggcttca gtcgtggtcc tctgggagca tccaggctgg ggtggtttgg caaggggctt
     1741 actagaccat catgtgcaga gagcagcaga gccccttgta caaaaaccag gacccctggg
     1801 ttccctgtgg ataggatgag ctgccaagga gatgtcactc ttggcaccct ccaagaatca
     1861 ttacggccgg gtgcagtggc tcatgcttga aatcccagca ctttgggagg ccgaggcggg
     1921 tgaatcacct gaggtcagga gttcgagacc agcctggcca acatggtgaa atcctgtctc
     1981 tactaaaaat acaaaattag ccaggcatgg tggcatgtgc ctgtaatccc agctactcgg
     2041 gaggctgagg caggagaatc acttgaaccc gggaggcgga ggttgcagtc agccaagatt
     2101 gcgccactgt actccagcct gggcaacaag agtgaaactc catctcaaaa aaaaaaaaaa
     2161 aaaaatacag aattagccag gcatggtggc gtgtgcctgt aatcccagct actcaagagg
     2221 ctgaggcagg agaatcactt gaacctggga ggcagaggtt gcagtgagcc gagatcgcac
     2281 cattgcactc ctgcctgggc gacagagcga gactccgtct caaaaaaaaa gaaccgtgag
     2341 cactttgttt ctcttgcttc ttccacttca ctcttcttca gccagggcag ccaaaggcag
     2401 gatgggtgct tcccggatcc accagagggg gaaaggggtt gggcgtgtat gggggcagaa
     2461 cttttcccta gtgagagcct tggtgaggtc tgctggaggc acactgggga cacaggcaag
     2521 catcaggtgg tcccaacttc ccacccccct cccataaacg gcacttggct cagtctccct
     2581 ctgcccacct tgtcaccatg gagctgtggc attcaagcat ccaaatgccc tgcttctgtc
     2641 aggcctgccc cgtcgggtgc tttgtttact tgtcaggttg ggcagatggt ctcaagccct
     2701 ggttggtggg tgggtgagtg aggagacagg ttagacaggg gaaggtccca ggacatgtct
     2761 tccctctctg ggctgacctg aggcagtgga ggctctcagg tgtgggatgg ggttttcagg
     2821 ctgggatgtt ctgtaccgta gaggataggc cttcctatta cttatcggag cactacggga
     2881 gggcaggtcc ctccccaggg tgtttaacac tggaggctgc agggtcagga ggagaatcgt
     2941 ggggccagga gggcagaggc acactccatc ttcgtgctcc tcacaggccc tgcctccctg
     3001 cctgctaagg acacagggaa gggggtcccc acctcagtgc ctgcctccct tccctgtgcc
     3061 tgtgtacctg gcagtcacag ccacctggcg tgtcccagaa accaaccggc tgacctcatc
     3121 tcctgcccgg ccccacctcc attggctttg gcttttggcg tttgtgctgc ccgacccttt
     3181 ctcctgtccg gatgcgcagg gcagggcctg agccgtcgag ctgcacccac agcaggctgc
     3241 ctttggtgac tcaccgggtg aacgggggca ttgcgaggca tcccctccct gggtttggct
     3301 cctgcccacg gggctgacag tagaaatcac aggctgtgag acagctggag cccagctctg
     3361 cttgaaccta ttttaggtct ctgatccccg cttcctcttt agactcccct agagctcagc
     3421 cagtgctcaa cctgaggctg ggggtctctg aggaagagtg agttggagct gaggggtctg
     3481 gggctgtccc ctgagagagg ggccagaggc agtgtcaaga gccgggcagt ctgattgtgg
     3541 ctcaccctcc atcactccca ggggcccctg gcccagcagc cgcagctccc aaccacaata
     3601 tcctttgggg tttggcctac ggagctgggg cggatgaccc ccaaatagcc ctggcagatt
     3661 ccccctagac ccgcccgcac catggtcagg catgcccctc ctcatcgctg ggcacagccc
     3721 agagggtata aacagtgctg gaggctggcg gggcaggcca gctgagtcct gagcagcagc
     3781 ccagcggatc tcgaggaact gaaaaaccag aaagttaact ggtaagttta gtctttttgt
     3841 cttttatttc ggatccggtg gtggtgcaaa tcaaagaact gctcctcagt ggatgttgcc
     3901 tttacttcta ggcctgtacg gaagtgttac ttctgctcta aaagctgctg caggtcttag
     3961 ttaactaaga ctctagggaa ttgcaccggc cacaaccatg gtgagcaagg gcgaggagct
     4021 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     4081 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     4141 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
     4201 cgtgcagtgc ttcgcccgct accccgacca catgcgccag cacgacttct tcaagtccgc
     4261 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     4321 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     4381 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     4441 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     4501 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     4561 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct
     4621 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     4681 cgggatcact ctcggcatgg acgagctgta caagtaactc gctagagtcg gggcggccgg
     4741 cccttgcagg aattctgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     4801 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     4861 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     4921 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatgga
     4981 attcgatatc aagctagcaa ttcgagctcg gtacccgggg atcccctgga gcccaggagg
     5041 gaggtgtgtg agctcaatcc ggactgtgac gagttggctg accacatcgg ctttcaggag
     5101 gcctatcggc gcttctacgg cccggtctag ggtgtcgctc tgctggcctg gccggcaacc
     5161 ccagttctgc tcctctccag gcacccttct ttcctcttcc ccttgccctt gccctgacct
     5221 cccagcccta tggatgtggg gtccccatca tcccagctgc tcccaaataa actccagaag
     5281 aggaatctgt gggcctgtga gtctgtccag tttatggagt gtgggaggga ggtgtcagga
     5341 ggatgggggt gaggaggttt taccttcttc agttctagaa agtgctttcc aaagttttat
     5401 ttttttattt gtacctgcct tgttccagaa aacattgaag gtggcttccc aaagtctaac
     5461 tagggatacc ccctctagcc taggaccctc ctccccacac ctcaatccac caaaccatcc
     5521 ataatgcacc cagataggcc cacccccaaa agcctggaca ccttgagcac acagttatga
     5581 ccaggacaga ctcatctcta taggcaaata gctgctggca aactggcatt acctggtttg
     5641 tggggatggg ggggcaagtg tgtggcctct cggcctggtt agcaagaagc attcagggta
     5701 ggcctaggtt agtcgtgtta gttcttccct gtgctgagca gagacttcca gaagcaccag
     5761 aaacggagcc agatgaaagg accccaacac ctccccccgc caacctttga cagaatatag
     5821 gggcatcttc agcctggaca cgcatgcatc tcccctctca gaccctcagc acttcttcca
     5881 ctcccatcaa gagccccctc acggtccctc tcacactctg ccagtccccc tagacacccc
     5941 tcctcttctc tgccctctct cctgtgccct ctctcctcag cccctgttgg ttccaggctg
     6001 agatgcgtcc ccacctgatt aggcccaaat ctgggctcct cgtcagcact ggggcctggc
     6061 ctctgccccc tccaggacag ggtcagggat ggggccttac tgttgtttgg cctgggtacc
     6121 cccctccagt gggccaccct gcagcagagg gcatgtactg ggggcccgaa gcagggtggc
     6181 tgtgaaagca gcaataatga gtaggttccc agctgcagcc aagaccagtg tggccactgt
     6241 gcctgccagg cccagggcag gttcctgtgt ggccagccag gctctgcgtg atcccatatc
     6301 agccagcact gcctccagct ggaagtgggt gcccagcact gcacagatgt ggaataactg
     6361 gtggctgtgg cctgtggaga ggatgggcag gtcagagcca cacctttccc agtgcaagtc
     6421 accccatcct ctgggcctgc tactccccca taaattgaga ataagaaacc ctgactccca
     6481 gagtgactga aaggaatgaa tgagcatgtg tgcatgtggc cccagcacac atgctactag
     6541 ttttgctacc tgagaagtgg caggtggagg acccttccag tgatgctctc gttataggaa
     6601 gacagggaag tccctcctat aatttgcctc catcctgggg ctgtaatacc catttaccca
     6661 gttcattctg tgctctggga aggcatctgc ccctgcctct tcccgggccg ggccaggcgt
     6721 gccctcaccg atgtaatcaa agcgtcctgg tgccagcctt tcaggcaggt gggaggcgaa
     6781 gaggaagcca gtgagcagcg cgcagaagag atggtagcca tggctggtgc tcagggcctc
     6841 ctgcccacag ccgtggcccc tgccccagca cagcccgagc tgtcaaagga aaaccaaggc
     6901 tgctggggag ggactcggga gccgaaacat ggggtgtgtc tgggatctgg tgaggacgca
     6961 gagcgggcgg gcacagctag gcggggtgct ggaccccaag agaggcggct gggctgaggc
     7021 agggactgag ggggcagggc tggaagtggg caccttgagg gtcaggacag gaaagcagga
     7081 gtctgggcag gggccctagg cctccttacc cgataaaaga gtgggaggtt gtcgaacagg
     7141 aatggatagg cgaaggctcc tgtgcggagg accttactga gcccagggct ttccagctcc
     7201 aggaaactgg gaggcgggag gaaaaggccg gtcaccatcg ctgtgcttgg ggtaggggtc
     7261 agagaaaggc aagaggactt gggcaaagat ggaatatcag cacagcctgc agctaagaaa
     7321 accgttaggg catatgacct cctgtgtaaa ggaccacatc tgtatgtggg gataaagggt
     7381 ctgacagagg aagtcctcag tacatggtcc cgatgcctcc agtgctgttc gagagcagtg
     7441 aggtgtgtcc tctcctgtgc ctccgccttc cccgtcccca ctggaggggc ctgggaaccc
     7501 accgggagta gcaggagagg ccggtgcaca ggaaggagtt gagtgcggcg gcaggcacaa
     7561 agaactggtg caggtggccg tgcagccagg aggccggcat ggagtaggcg gcatagggga
     7621 aggcgcagcc tgcggtgagg tgggggcgtg cagctgaggc ctgaggggcg cgcccctatg
     7681 tcccgcgtcc cacccctcgc cccgctccgg gacgcccaga ccccgcgctc ccgcgcctgt
     7741 ccggctcacc cagactgtag aggctgagcg cgccgtagtc gaggaagtag cagatgtggc
     7801 gcatgcgggg cgacatggag ctgaaggtgt gcgcgcagca cgacgcgaag gggtagaggc
     7861 aggcgggcag caggaagacc agcagcggcc agtggtacgg ctccgcacgg aagccggggc
     7921 cgcccgccag cgccaggagc cgccacagga agtacctgcg gcgggcgcgt gctcaggccg
     7981 ccgcggaccc ccgcgactgc cgctgcagcc cgtgggcctg gacggtccct gcccgcctgg
     8041 gtaccccctc tgagctcaag gccgcggcct ggggcagagc ctcccctcac caggtgggca
     8101 ggaagtgagt ccagatgttg accgtctcgt tggtcatctg gaaggagctg aggacacagt
     8161 ccaaagccga gctggtgggg cggcggtagc cagacatgat gccatcttcc cagaacacct
     8221 agggtagtgg tgggagagga agggcgtcac tgtgcccgga ccctttccct ccatctgaga
     8281 tctctggaag cagggtcaaa gaggctgggc tggagatcaa cgtggccccc aagccaaact
     8341 aaatctgatc tgatgttacc acaggtccca gtacccacaa cccacaaaac aaataggaaa
     8401 ccgaagagcc agattggggt gggatggggg tggagcgcag cacacagggc gctctgggga
     8461 aggggactcc agatggaggg ccacaagctt gcatgcctgc aggtcgggtt tgcgtattgg
     8521 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8581 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8641 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8701 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8761 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8821 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8881 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8941 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9001 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9061 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9121 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     9181 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9241 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9301 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9361 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9421 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9481 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9541 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9601 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9661 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9721 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9781 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9841 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9901 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9961 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10021 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10081 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10141 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10201 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10261 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10321 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10381 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10441 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    10501 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
    10561 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    10621 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
    10681 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
    10741 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
    10801 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gt
//