| 质粒名称: | pYK474 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000820 |
| 产品用途: | Mammalian PhCMV-driven constitutive 4xANR-mCherry-CAAX expression vector (PhCMV-4xANR-mCherry-CAAX-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | 4xANR-mCherry-CAAX |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK474
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK474载体质粒基本信息
pYK474质粒图谱载体图谱和pYK474载体序列质粒序列多克隆位点信息
pYK474质粒载体简介
pYK474质粒序列载体序列
LOCUS pYK474 6869 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6869)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6869
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 881..931
/label=oYK474_ANR_F
primer_bind 881..900
/label=oYK31-mCherry-NS3a-F
primer_bind 904..954
/label=olyl215
misc_feature 904
/label=NheI
primer_bind complement(912..935)
/label=olyl323
primer_bind 915..954
/label=olyl211
misc_feature 915
/label=New Feature
misc_feature 915
/label=New Feature
CDS 929
/codon_start=1
/label=5xGS Linker
/translation=""
CDS 930..992
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 930..962
/label=olyl235
CDS 993..1067
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind 1016..1025
/label=oYK474_mCherry_F
CDS 1068..1130
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 1068..1083
/label=Primer 4
primer_bind 1115..1129
/label=Primer 3
CDS 1131..1205
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind 1131..1149
/label=Primer 1
primer_bind complement(1180..1209)
/label=olyl234
primer_bind complement(1180..1205)
/label=olyl212
primer_bind 1190..1244
/label=olyl235
CDS 1212..1274
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 1212..1236
/label=olyl211
primer_bind 1212..1236
/label=olyl215
CDS 1275..1349
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind 1298..1307
/label=oYK474_mCherry_F
CDS 1350..1412
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 1350..1365
/label=Primer 4
primer_bind 1397..1411
/label=Primer 3
CDS 1413..1487
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind 1413..1431
/label=Primer 1
primer_bind complement(1462..1505)
/label=olyl212
primer_bind complement(1462..1505)
/label=olyl234
primer_bind 1472..1491
/label=olyl235
primer_bind complement(1481..1516)
/label=oYK506_ANR_R
primer_bind complement(1481..1505)
/label=oYK474_ANR4_R
primer_bind 1486..1525
/label=oYK474_mCherry2_F
primer_bind 1486..1523
/label=oYK474_mCherry_F
primer_bind complement(1488..1505)
/label=olyl236
misc_feature 1494..1505
/label=NSP3
CDS 1505..1508
/codon_start=1
/label=GNCR1-N
/translation="S"
primer_bind complement(1508..1534)
/label=oDJ171-4
primer_bind 1509..1586
/label=oDJ171-3
primer_bind complement(1510..1558)
/label=oLZ145 DNCR2-GNCR1 R
misc_feature 1525..1543
/label=linker
primer_bind 1529..1581
/label=oYK03-02-F
primer_bind complement(1536..1559)
/label=oYK421_GNCR3_R
primer_bind 1540..1577
/label=oYK421_mCherry_F
misc_feature 1544
/label=linker
misc_feature 1545..1559
/label=NSP3
primer_bind 1560..1583
/label=oXQ135
primer_bind 1560..1583
/label=oXQ174
primer_bind 1560..1583
/label=omz_mCherry_F
primer_bind 1560..1580
/label=oYK31-mCherry-NS3a-F
CDS 1563..2267
/codon_start=1
/label=mCherry
/translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
GRHSTGGMDELYK"
primer_bind 1563..1589
/label=omz_linker_mCherry_F
primer_bind 1604..1622
primer_bind 1604..1622
/label=DDL7AeF
/note="KpnI"
primer_bind 1604..1622
/label=L7Ae FLP
primer_bind complement(1875..1886)
/label=oSCL011
primer_bind complement(1875..1886)
/label=omz_Coh2_R
primer_bind complement(1875..1886)
/label=omz_Coh2_His_R
primer_bind 2008..2024
/label=mCherry_F测序
primer_bind 2230..2284
/label=oYF005
primer_bind complement(2247..2366)
/label=oYK316_mCherry_CAAX_R
primer_bind complement(2247..2287)
/label=oDJ171-4
primer_bind complement(2247..2270)
/label=oXQ136
primer_bind complement(2247..2267)
/label=oXQ161
primer_bind complement(2247..2267)
/label=omz_mCherry_His_R
primer_bind complement(2247..2267)
/label=oYK56-mCherry-R
primer_bind complement(2247..2267)
/label=oYK58-mCherry-R
primer_bind complement(2249..2278)
/label=omz_mCherry_linker_R
primer_bind complement(2255..2273)
/label=oYK431_NES2_R
primer_bind complement(2260..2279)
/label=pYK472_mCherry_R
CDS 2286..2291
/codon_start=1
/label=4A/4B
/translation="DV"
misc_feature 2304..2363
/label=CAAX
primer_bind complement(2323..2389)
/label=oYK421_CAAX_R
primer_bind complement(2364..2387)
/label=oYK431_NES2_R
primer_bind complement(2367..2389)
/label=oYK57-MCP-mCherry-R
primer_bind complement(2390..2407)
/label=oXQ161
terminator 2463..2690
/label=bGH PA
misc_feature 2736..3164
/label=f1 origin
misc_feature 3169..3512
/label=SV40 early promoter and origin
misc_feature 3358..3435
/label=SV40 ORI
CDS 3577..4368
/label=Kan/neoR
misc_feature 4542..4672
/label=SV40 early polyadenylation signal
primer_bind complement(4708..4728)
/label=M13-rev
misc_binding complement(4734..4756)
/label=LacO
promoter complement(4761..4790)
/label=lac
misc_feature complement(5055..5728)
/label=pUC origin
CDS complement(5876..6535)
/label=AmpR
promoter complement(6775..6803)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 caccgtttaa acttaagctt gccaccatgg gggagctcga cgagttggtt tatctcttag
961 acggacccgg atatgacccc attcattcag atggctccgg aacgggcagc gggaccggaa
1021 gtggcaccgg aaccacttcc ggaaccggta ctggcgggag taccggcggt gagctagacg
1081 aactcgtgta cctgttggat ggccccggtt acgacccaat ccacagcgat ggaagtggaa
1141 ctgggtctgg gactgggtcc ggcaccggga ctacttcagg caccggcact gggggctcca
1201 ctggccatac cggggagctc gacgagttgg tttatctctt agacggaccc ggatatgacc
1261 ccattcattc agatggctcc ggaacgggca gcgggaccgg aagtggcacc ggaaccactt
1321 ccggaaccgg tactggcggg agtaccggcg gtgagctaga cgaactcgtg tacctgttgg
1381 atggccccgg ttacgaccca atccacagcg atggaagtgg aactgggtct gggactgggt
1441 ccggcaccgg gactacttca ggcaccggca ctgggggctc cactggccat atgatgctca
1501 agatgagcgc cagcggaagt ggcggaggag gcgacgtcgc tagcatgctc aagatggaga
1561 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
1621 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
1681 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
1741 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
1801 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
1861 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
1921 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
1981 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
2041 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
2101 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
2161 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
2221 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaaggga agcggcggag
2281 gaggcgacgt cggcagcggc agcaaaatga gtaaggatgg aaagaaaaag aaaaagaaaa
2341 gcaaaacaaa gtgtgtaata atgtaaggat ccactagtcc agtgtggtgg aattctgcag
2401 atatccagca cagtggcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc
2461 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
2521 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
2581 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
2641 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
2701 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
2761 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
2821 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
2881 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
2941 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
3001 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
3061 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
3121 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
3181 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
3241 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
3301 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
3361 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
3421 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
3481 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
3541 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
3601 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
3661 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
3721 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
3781 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
3841 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
3901 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
3961 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
4021 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
4081 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
4141 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
4201 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
4261 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
4321 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
4381 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
4441 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
4501 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
4561 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
4621 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
4681 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
4741 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
4801 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
4861 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
4921 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
4981 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
5041 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
5101 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
5161 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
5221 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
5281 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
5341 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
5401 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
5461 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
5521 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
5581 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
5641 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
5701 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
5761 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
5821 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
5881 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
5941 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
6001 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
6061 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
6121 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
6181 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
6241 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
6301 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
6361 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
6421 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
6481 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
6541 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
6601 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
6661 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
6721 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
6781 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
6841 catttccccg aaaagtgcca cctgacgtc
//
pYK474载体图谱质粒图谱pdf版和pYK474载体序列质粒序列等相关资料下载
pYK474质粒载体应用举例
- 上一篇：pYK472
- 下一篇：没有了
