C5V

LOCUS       C5V	5431 bp 	DNA	SYN
DEFINITION  C5V
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="C5V"
                     /mol_type="other DNA"
     gene            6..719
                     /label="ECFP (variant)"
                     /gene="ECFP (variant)"
     misc_feature    complement(48..69)
                     /label="EGFP_N_primer"
     CDS             237..767
                     /label="ORF frame 3"
     misc_feature    456..539
                     /label="M153T,V163A,S175G (Venus)"
     misc_feature    656..677
                     /label="EGFP_C_primer"
     terminator      773..1001
                     /label="SV40_PA_terminator"
     misc_feature    971..990
                     /label="EBV_rev_primer"
     rep_origin      complement(1140..1446)
                     /label="f1_origin"
     promoter        1525..1553
                     /label="AmpR_promoter"
     misc_feature    complement(1619..1639)
                     /label="pBABE_3_primer"
     misc_feature    complement(1625..1840)
                     /label="SV40_enhancer"
     promoter        1637..1905
                     /label="SV40_promoter"
     rep_origin      1804..1881
                     /label="SV40_origin"
     misc_feature    1866..1885
                     /label="SV40pro_F_primer"
     CDS             1988..2782
                     /label="ORF frame 2"
     gene            1991..2779
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
     CDS             complement(2297..2833)
                     /label="ORF frame 1"
     terminator      2957..3226
                     /label="TK_PA_terminator"
     rep_origin      3374..3993
                     /label="pBR322_origin"
     promoter        4102..4654
                     /label="CMV_immearly_promoter"
     misc_feature    4157..4444
                     /label="CAG_enhancer"
     misc_feature    4611..4631
                     /label="CMV_fwd_primer"
     promoter        4612..4681
                     /label="CMV_promoter"
     CDS             complement(4690..779)
                     /label="ORF frame 3"
     CDS             4705..767
                     /label="ORF frame 1"
     gene            4708..5421
                     /label="ECFP"
                     /gene="ECFP"
     misc_feature    complement(4750..4771)
                     /label="EGFP_N_primer"
     misc_feature    4888..4917
                     /label="Y66W (ECFP)"
     misc_feature    5358..5379
                     /label="EGFP_C_primer"
ORIGIN
    1 GATCTGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG
   61 ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT
  121 ACGGCAAGCT GACCCTGAAG CTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA
  181 CCCTCGTGAC CACCCTCGGC TACGGCCTGC AGTGCTTCGC CCGCTACCCC GACCACATGA
  241 AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT
  301 TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC
  361 TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC
  421 ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CACCGCCGAC AAGCAGAAGA
  481 ACGGCATCAA GGCCAACTTC AAGATCCGCC ACAACATCGA GGACGGCGGC GTGCAGCTCG
  541 CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC
  601 ACTACCTGAG CTACCAGTCC AAGCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG
  661 TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGG
  721 AATTCTGCAG TCGACGGTAC CGCGGGCCCG GGATCCACCG GATCTAGATA ACTGATCATA
  781 ATCAGCCATA CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC
  841 CTGAACCTGA AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT
  901 AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG
  961 CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT AACGCGTAAA TTGTAAGCGT
 1021 TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA
 1081 GGCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG ACCGAGATAG GGTTGAGTGT
 1141 TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG TCAAAGGGCG
 1201 AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT CAAGTTTTTT
 1261 GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC
 1321 TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG
 1381 CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT
 1441 TAATGCGCCG CTACAGGGCG CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC
 1501 TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG
 1561 ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTCCTGAG GCGGAAAGAA CCAGCTGTGG
 1621 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA
 1681 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC
 1741 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG
 1801 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT
 1861 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA
 1921 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAGATCGAT CAAGAGACAG GATGAGGATC
 1981 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
 2041 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
 2101 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
 2161 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
 2221 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
 2281 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
 2341 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
 2401 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
 2461 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
 2521 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
 2581 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
 2641 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
 2701 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
 2761 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG
 2821 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC
 2881 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG
 2941 TTCTTCGCCC ACCCTAGGGG GAGGCTAACT GAAACACGGA AGGAGACAAT ACCGGAAGGA
 3001 ACCCGCGCTA TGACGGCAAT AAAAAGACAG AATAAAACGC ACGGTGTTGG GTCGTTTGTT
 3061 CATAAACGCG GGGTTCGGTC CCAGGGCTGG CACTCTGTCG ATACCCCACC GAGACCCCAT
 3121 TGGGGCCAAT ACGCCCGCGT TTCTTCCTTT TCCCCACCCC ACCCCCCAAG TTCGGGTGAA
 3181 GGCCCAGGGC TCGCAGCCAA CGTCGGGGCG GCAGGCCCTG CCATAGCCTC AGGTTACTCA
 3241 TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC
 3301 CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA
 3361 GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC
 3421 TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA
 3481 CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT
 3541 CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC
 3601 GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG
 3661 TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG
 3721 TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG
 3781 CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC
 3841 AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT
 3901 AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG
 3961 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC
 4021 TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT
 4081 ACCGCCATGC ATTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA
 4141 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA
 4201 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT
 4261 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT
 4321 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA
 4381 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT
 4441 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT
 4501 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA
 4561 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG
 4621 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT
 4681 CCGCTAGCGC TACCGGTCGC CACCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG
 4741 GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC
 4801 GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC
 4861 AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTGGGGCGT GCAGTGCTTC
 4921 GCCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC
 4981 TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG
 5041 GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG
 5101 GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACG CCATCAGCGA CAACGTCTAT
 5161 ATCACCGCCG ACAAGCAGAA GAACGGCATC AAGGCCAACT TCAAGATCCG CCACAACATC
 5221 GAGGACGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC
 5281 CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT CCAAGCTGAG CAAAGACCCC
 5341 AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC
 5401 GGCATGGACG AGCTGTACAA GTCCGGACTC A
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