| 质粒名称: | pHCMV-EcoEnv |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 15802 |
| 存储实验室: | Miguel Sena-Esteves |
|
载体骨架基本信息 |
|
| 载体名称: | pHCMV |
| 载体出品公司: | Clontech |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 4768 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | MLV env (ecotropic) |
| 物种来源: | Moloney murine leukemia virus |
| 插入大小: | 1995 bp |
| GenBank编号 | AF033811 |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | |
| 3' 克隆位点: | |
| 5' 测序引物 | N/A |
| 3' 测序引物 | N/A |
|
文献引用方法 |
|
| 材料和方法部分: | pHCMV-EcoEnv was a gift from Miguel Sena-Esteves (Addgene plasmid # 15802) |
| 参考文献部分: | Optimized large-scale production of high titer lentivirus vector pseudotypes. Sena-Esteves M, Tebbets JC, Steffens S, Crombleholme T, Flake AW. J Virol Methods. 2004 Dec 15. 122(2):131-9. 10.1016/j.jviromet.2004.08.017 PubMed 15542136 |
买家导航
pHCMV-EcoEnv载体质粒基本信息
pHCMV-EcoEnv质粒图谱载体图谱和pHCMV-EcoEnv载体序列质粒序列多克隆位点信息
pHCMV-EcoEnv质粒载体简介
pHCMV-EcoEnv质粒序列载体序列
LOCUS pHCMV-EcoEnv 6763 bp DNA SYN
DEFINITION pHCMV-EcoEnv
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..6763
/organism="pHCMV-EcoEnv"
/mol_type="other DNA"
promoter 98..674
/label="CMV_immearly_promoter"
misc_feature 162..988
/label="5_LTR"
misc_feature 177..464
/label="CAG_enhancer"
misc_feature 631..651
/label="CMV_fwd_primer"
promoter 632..701
/label="CMV_promoter"
promoter 644..763
/label="CMV2_promoter"
misc_feature 675..694
/label="pCEP_fwd_primer"
misc_feature 677..701
/label="LNCX_primer"
misc_feature 792..1364
/label="bGlob_int"
CDS 1455..3452
/label="ORF frame 3"
terminator 3471..3913
/label="rb_glob_PA_terminator"
misc_feature complement(3958..3980)
/label="M13_pUC_rev_primer"
promoter complement(3994..4023)
/label="lac_promoter"
gene complement(5106..5967)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(5485..5967)
/label="ORF frame 2"
promoter complement(6009..6037)
/label="AmpR_promoter"
misc_feature complement(6554..6709)
/label="lacZ_a"
misc_feature 6680..6702
/label="M13_pUC_fwd_primer"
promoter 6695..6711
/label="M13_forward20_primer"
promoter 6721..6739
/label="T7_promoter"
ORIGIN
1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
721 gctgttttga cctccataga agacaccggg accgatccag cctccggtcg accgatcctg
781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
1381 tggttgttgt gctgtctcat cattttggca aagaattccg cgggcggccg ctctagagga
1441 tccgctagac tgacatggcg cgttcaacgc tctcaaaacc ccttaaaaat aaggttaacc
1501 cgcgaggccc cctaatcccc ttaattcttc tgatgctcag aggggtcagt actgcttcgc
1561 ccggctccag tcctcatcaa gtctataata tcacctggga ggtaaccaat ggagatcggg
1621 agacggtatg ggcaacttct ggcaaccacc ctctgtggac ctggtggcct gaccttaccc
1681 cagatttatg tatgttagcc caccatggac catcttattg ggggctagaa tatcaatccc
1741 ctttttcttc tcccccgggg cccccttgtt gctcaggggg cagcagccca ggctgttcca
1801 gagactgcga agaaccttta acctccctca cccctcggtg caacactgcc tggaacagac
1861 tcaagctaga ccagacaact cataaatcaa atgagggatt ttatgtttgc cccgggcccc
1921 accgcccccg agaatccaag tcatgtgggg gtccagactc cttctactgt gcctattggg
1981 gctgtgagac aaccggtaga gcttactgga agccctcctc atcatgggat ttcatcacag
2041 taaacaacaa tctcacctct gaccaggctg tccaggtatg caaagataat aagtggtgca
2101 accccttagt tattcggttt acagacgccg ggagacgggt tacttcctgg accacaggac
2161 attactgggg cttacgtttg tatgtctccg gacaagatcc agggcttaca tttgggatcc
2221 gactcagata ccaaaatcta ggaccccgcg tcccaatagg gccaaacccc gttctggcag
2281 accaacagcc actctccaag cccaaacctg ttaagtcgcc ttcagtcacc aaaccaccca
2341 gtgggactcc tctctcccct acccaacttc caccggcggg aacggaaaat aggctgctaa
2401 acttagtaga cggagcctac caagccctca acctcaccag tcctgacaaa acccaagagt
2461 gctggttgtg tctagtagcg ggacccccct actacgaagg ggttgccgtc ctgggtacct
2521 actccaacca tacctctgct ccagccaact gctccgtggc ctcccaacac aagttgaccc
2581 tgtccgaagt gaccggacag ggactctgca taggagcagt tcccaaaaca catcaggccc
2641 tatgtaatac cacccagaca agcagtcgag ggtcctatta tctagttgcc cctacaggta
2701 ccatgtgggc ttgtagtacc gggcttactc catgcatctc caccaccata ctgaacctta
2761 ccactgatta ttgtgttctt gtcgaactct ggccaagagt cacctatcat tcccccagct
2821 atgtttacgg cctgtttgag agatccaacc gacacaaaag agaaccggtg tcgttaaccc
2881 tggccctatt attgggtgga ctaaccatgg ggggaattgc cgctggaata ggaacaggga
2941 ctactgctct aatggccact cagcaattcc agcagctcca agccgcagta caggatgatc
3001 tcagggaggt tgaaaaatca atctctaacc tagaaaagtc tctcacttcc ctgtctgaag
3061 ttgtcctaca gaatcgaagg ggcctagact tgttatttct aaaagaagga gggctgtgtg
3121 ctgctctaaa agaagaatgt tgcttctatg cggaccacac aggactagtg agagacagca
3181 tggccaaatt gagagagagg cttaatcaga gacagaaact gtttgagtca actcaaggat
3241 ggtttgaggg actgtttaac agatcccctt ggtttaccac cttgatatct accattatgg
3301 gacccctcat tgtactccta atgattttgc tcttcggacc ctgcattctt aatcgattag
3361 tccaatttgt taaagacagg atatcagtgg tccaggctct agttttgact caacaatatc
3421 accagctgaa gcctatagag tacgagccat agctcgaggg cccagatctt tttccctctg
3481 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
3541 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
3601 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
3661 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
3721 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
3781 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
3841 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
3901 tcttatggag atccctcgac ggatcggccg caattcgtaa tcatgtcata gctgtttcct
3961 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
4021 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
4081 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
4141 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
4201 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
4261 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
4321 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
4381 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
4441 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
4501 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
4561 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
4621 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
4681 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
4741 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
4801 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
4861 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
4921 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
4981 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
5041 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
5101 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
5161 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
5221 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
5281 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
5341 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
5401 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
5461 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
5521 aaagcgggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
5581 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
5641 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
5701 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
5761 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
5821 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
5881 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
5941 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
6001 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
6061 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
6121 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
6181 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
6241 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
6301 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
6361 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
6421 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
6481 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
6541 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
6601 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
6661 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg
6721 taatacgact cactataggg cgaattggag ctccaccgcg gtg
//
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pHCMV-EcoEnv质粒载体应用举例
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