| 质粒名称: | pAAV-hSyn-GRAB_NE1h |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000120 |
| 产品用途: | Expresses the NE sensor GRAB_NE1h in neurons |
| 存储实验室: | Yulong Li Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | hSyn |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | GRAB_NE1h |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A Genetically Encoded Fluorescent Sensor for Rapid and Specific In Vivo Detection of Norepinephrine
Feng J., Zhang C., Lischinsky J.E., Jing M., Zhou J., Wang H., Zhang Y., Dong A., Wu Z., Wu H., Chen W., Zhang P., Zou J., Hires S., Zhu J., Cui G., Lin D., Du J., Li Y.
Neuron 102, 745-761.e8 (2019)
|
买家导航
pAAV-hSyn-GRAB_NE1h载体质粒基本信息
pAAV-hSyn-GRAB_NE1h质粒图谱载体图谱和pAAV-hSyn-GRAB_NE1h载体序列质粒序列多克隆位点信息
pAAV-hSyn-GRAB_NE1h质粒载体简介
pAAV-hSyn-GRAB_NE1h质粒序列载体序列
LOCUS pAAV-hSyn-GRAB_NE1h 6482 bp DNA circular SYN 21-APR-2023
DEFINITION Expresses the genetically-encoded fluorescent norepinephrine(NE)
sensor GRAB_NE1h in neurons.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6482)
AUTHORS Feng J, Zhang C, Lischinsky JE, Jing M, Zhou J, Wang H, Zhang Y,
Dong A, Wu Z, Wu H, Chen W, Zhang P, Zou J, Hires SA, Zhu JJ, Cui G,
Lin D, Du J, Li Y
TITLE A Genetically Encoded Fluorescent Sensor for Rapid and Specific In
Vivo Detection of Norepinephrine.
JOURNAL Neuron. 2019 May 22;102(4):745-761.e8. doi:
10.1016/j.neuron.2019.02.037. Epub 2019 Mar 25.
PUBMED 30922875
REFERENCE 2 (bases 1 to 6482)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 27, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1016/j.neuron.2019.02.037"; journalName: "Neuron"; date:
"2019-05-22- 22"; volume: "102"; issue: "4"; pages: "745-761.e8"
FEATURES Location/Qualifiers
source 1..6482
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 46..175
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
promoter 176..619
/label=hSyn
regulatory 626..635
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
sig_peptide 632..694
/codon_start=1
/product="leader sequence from mouse immunoglobulin kappa
light chain"
/label=Ig-kappa leader
/translation="METDTLLLWVLLLWVPGSTGD"
protein_bind 695..719
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 719..2629
/codon_start=1
/label=GRAB_NE1h
/translation="TTGGSLQPDAGNASWNGTEAPGGGARATPYSLQVTLTLVCLAGLL
MLLTVFGNVLVIIAVFTSRALKAPQNLFLVSLASADILVATLVIPFSLANEVMGYWYFG
KAWCEIYLALDVLFCTSSIVHLCAISLDRYWSITQAIEYNLKRTPRRIKAIIITVWVIS
AVISFPPLISIEKKGGGGGPQPAEPRCEINDQKWYVISSCIGSFFAPCLIMILVYVRIY
QIAKRRTRVPPSRRGPDAVAAPPGGTERRPNGLGPERSAGPGGAEAEPLPTQLNGAPGE
PAPAGPRDTDALDLEEGGNVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIG
DGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVRK
GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVT
TLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVN
RIELKGIDFKEDGNILGHKLEYNTGAAARWRGRQNREKRFKFVLAVVIGVFVVCWFPFF
FTYTLTAVGCSVPRTLFKFFFWFGYCNSSLNPVIYTIFNHDFRRAFKKILCRGDRKRIV
L"
primer_bind 1288..1309
/label=WKG120-F
primer_bind 1822..1843
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(1949..1970)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2210..2229)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
misc_feature 2654..3242
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2707..2727)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3125..3136)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3274..3750
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind complement(3473..3492)
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
repeat_region 3790..3930
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 3790..3919
/label=AAV2 ITR
rep_origin 4005..4460
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4092..4111)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4302..4323
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4477..4496)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4596..4618
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(4656..4674)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4742..4846
/gene="bla"
/label=AmpR promoter
CDS 4847..5707
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5065..5084)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5878..6466
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6367..6386
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cctttttacg gttcctggcc ttttgctggc cttttgctca catgtcctgc aggcagctgc
61 gcgctcgctc gctcactgag gccgcccggg cgtcgggcga cctttggtcg cccggcctca
121 gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg ttcctgcggc
181 cgcacgcgta tgaggcgggg tgggggtgcc tacctgacga ccgaccccga cccactggac
241 aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg gaggggaaac
301 aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt gccttcgccc
361 ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac gtcactcgcc
421 ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc cgccggccca
481 gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca tctgcgctgc
541 ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt cgtgtcgtgc
601 ctgagagcgc agtcgagaaa ccggtgccac catggagaca gacacactcc tgctatgggt
661 actgctgctc tgggttccag gttccactgg tgacacaagt ttgtacaaaa aagttggcac
721 caccggtggc tccctgcagc cggacgcggg caacgcgagc tggaacggga ccgaggcgcc
781 ggggggcggc gcccgggcca ccccttactc cctgcaggtg acgctgacgc tggtgtgcct
841 ggccggcctg ctcatgctgc tcaccgtgtt cggcaacgtg ctcgtcatca tcgccgtgtt
901 cacgagccgc gcgctcaagg cgccccaaaa cctcttcctg gtgtctctgg cctcggccga
961 catcctggtg gccacgctcg tcatcccttt ctcgctggcc aacgaggtca tgggctactg
1021 gtacttcggc aaggcttggt gcgagatcta cctggcgctc gacgtgctct tctgcacgtc
1081 gtccatcgtg cacctgtgcg ccatcagcct ggaccgctac tggtccatca cacaggccat
1141 cgagtacaac ctgaagcgca cgccgcgccg catcaaggcc atcatcatca ccgtgtgggt
1201 catctcggcc gtcatctcct tcccgccgct catctccatc gagaagaagg gcggcggcgg
1261 cggcccgcag ccggccgagc cgcgctgcga gatcaacgac cagaagtggt acgtcatctc
1321 gtcgtgcatc ggctccttct tcgctccctg cctcatcatg atcctggtct acgtgcgcat
1381 ctaccagatc gccaagcgtc gcacccgcgt gccacccagc cgccggggtc cggacgccgt
1441 cgccgcgccg ccggggggca ccgagcgcag gcccaacggt ctgggccccg agcgcagcgc
1501 gggcccgggg ggcgcagagg ccgaaccgct gcccacccag ctcaacggcg cccctggcga
1561 gcccgcgccg gccgggccgc gcgacaccga cgcgctggac ctggaggagg gaggaaacgt
1621 ctatatcaag gccgacaagc agaagaacgg catcaaggcg aacttccaca tccgccacaa
1681 catcgaggac ggcggcgtgc agctcgccta ccactaccag cagaacaccc ccatcggcga
1741 cggccccgtg ctgctgcccg acaaccacta cctgagcgtg cagtccaaac tttcgaaaga
1801 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
1861 tctcggcatg gacgagctgt acaagggcgg taccggaggg agcatggtga gaaagggcga
1921 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
1981 caagttcagc gtgtccggcg agggtgaggg cgatgccacc tacggcaagc tgaccctgaa
2041 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
2101 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
2161 gtccgccatg cccgaaggct acatccagga gcgcaccatc ttcttcaagg acgacggcaa
2221 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
2281 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacac
2341 cggagcagca gcacgctggc gcgggcggca gaaccgcgag aagcgcttca agttcgtgct
2401 ggccgtggtc atcggagtgt tcgtggtgtg ctggttcccc ttcttcttca cctacacgct
2461 cacggccgtc gggtgctccg tgccacgcac gctcttcaaa ttcttcttct ggttcggcta
2521 ctgcaacagc tcgttgaacc cggtcatcta caccatcttc aaccacgatt tccgccgcgc
2581 cttcaagaag atcctctgtc ggggggacag gaagcggatc gtgttgtgag aattcgatat
2641 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
2701 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
2761 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
2821 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
2881 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
2941 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
3001 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
3061 tccttggctg ctcgcctatg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
3121 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
3181 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
3241 gcatcgatac cgagcgctgc tcgagagatc tacgggtggc atccctgtga cccctcccca
3301 gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa
3361 ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg
3421 gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg
3481 gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt
3541 caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg
3601 ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc
3661 tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag
3721 gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt aggtaaccac gtgcggaccg
3781 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
3841 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
3901 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
3961 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
4021 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
4081 cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
4141 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
4201 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
4261 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
4321 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
4381 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
4441 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
4501 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
4561 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
4621 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
4681 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
4741 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
4801 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
4861 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
4921 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
4981 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
5041 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
5101 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
5161 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
5221 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
5281 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
5341 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
5401 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
5461 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
5521 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
5581 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
5641 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
5701 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
5761 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
5821 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
5881 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
5941 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
6001 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
6061 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
6121 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
6181 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
6241 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
6301 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
6361 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
6421 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
6481 gg
//
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