| 质粒名称: | pAAV-CaMKII-monArch-KGC-GFP-ER2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001082 |
| 产品用途: | voltage imaging |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: |
pAAV
|
| 表达系统: | 哺乳动物表达 |
| 启动子: |
CaMKII
|
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10, DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | monArch, GFP |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Engineering of Genetically Encoded Bright Near-Infrared Fluorescent Voltage Indicator
Xiao X., Yang A., Zhang H., Park D., Wang Y., Szabo B., Boyden E.S., Piatkevich K.D.
Int. J. Mol. Sci. 2025, Vol. 26, Page 1442 26, 1442 (2025)
|
pAAV-CaMKII-monArch-KGC-GFP-ER2
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-CaMKII-monArch-KGC-GFP-ER2载体质粒基本信息
pAAV-CaMKII-monArch-KGC-GFP-ER2质粒图谱载体图谱和pAAV-CaMKII-monArch-KGC-GFP-ER2载体序列质粒序列多克隆位点信息
pAAV-CaMKII-monArch-KGC-GFP-ER2质粒载体简介
pAAV-CaMKII-monArch-KGC-GFP-ER2质粒序列载体序列
LOCUS pAAV-CaMKII-monArch-KGC-GFP-ER2 6692 bp DNA circular SYN 12-MAR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6692)
AUTHORS Kiryl Piatkevich
TITLE Direct Submission
JOURNAL Exported Aug 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6692
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 34..223
/label=ITR
promoter 237..1525
/label=CaMKII promoter
/note="promoter for the mouse calcium/calmodulin-dependent
kinase II alpha subunit"
primer_bind 1511..1556
/label=0609-CaMKII-BamHI-monArch-Fw
misc_feature 1534
/label=MCS
/note="multiple cloning site"
regulatory 1535..1540
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1541..2299
/codon_start=1
/label=monArch
/translation="MVSIALQAGYDLLGDGRPEILWLGIGTLLILIGTFYFLVRGWGVT
NKDAREYYTVTILVSGIAGAAYLLMFFGIGLAEVSVGGEMLVIYYARYAEWLFCTPLLL
LDLALLAKVDRVIIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYVLA
TSLRSAAKERGPEVASTFNILTALVLVLWTAYPIIWIIGTEGAGVVGLGIETLLFMVLD
VTGKVGFGFILLRSRAILGVTEAPEPSAGAD"
primer_bind complement(2278..2368)
/label=0609-KGC-PP-monArch-Rv
primer_bind 2278..2368
/label=0609-monArch-PP-KGC-Fw
CDS 2348..2407
/codon_start=1
/label=KGC
/translation="KSRITSEGEYIPLDQIDINV"
CDS 2408..3121
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
CDS 3122..3142
/codon_start=1
/label=ER2
/translation="FCYENEV"
primer_bind complement(3140..3169)
/label=0609-WPRE-EcoRI-ER2-Rv
misc_feature 3170..3758
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
polyA_signal 3801..3922
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature 4025..4214
/label=ITR
misc_feature 4268..5208
/label=ColE1
/note="ColE1"
CDS complement(5209..6069)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6070..6174)
/gene="bla"
/label=AmpR promoter
rep_origin complement(6200..6655)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 gcgatcggtg cgggcctctt cgctattacg ccagctgcgc gctcgctcgc tcactgaggc
61 cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg
121 agcgcgcaga gagggagtgg ccaactccat cactaggggt tccttgtagt taatgattaa
181 cccgccatgc tacttatcta cgtagccatg ctctaggaag atcgtaggtt aattaacatt
241 atggccttag gtcacttcat ctccatgggg ttcttcttct gattttctag aaaatgagat
301 gggggtgcag agagcttcct cagtgacctg cccagggtca catcagaaat gtcagagcta
361 gaacttgaac tcagattact aatcttaaat tccatgcctt gggggcatgc aagtacgata
421 tacagaagga gtgaactcat tagggcagat gaccaatgag tttaggaaag aagagtccag
481 ggcagggtac atctacacca cccgcccagc cctgggtgag tccagccacg ttcacctcat
541 tatagttgcc tctctccagt cctaccttga cgggaagcac aagcagaaac tgggacagga
601 gccccaggag accaaatctt catggtccct ctgggaggat gggtggggag agctgtggca
661 gaggcctcag gaggggccct gctgctcagt ggtgacagat aggggtgaga aagcagacag
721 agtcattccg tcagcattct gggtctgttt ggtacttctt ctcacgctaa ggtggcggtg
781 tgatatgcac aatggctaaa aagcagggag agctggaaag aaacaaggac agagacagag
841 gccaagtcaa ccagaccaat tcccagagga agcaaagaaa ccattacaga gactacaagg
901 gggaagggaa ggagagatga attagcttcc cctgtaaacc ttagaaccca gctgttgcca
961 gggcaacggg gcaatacctg tctcttcaga ggagatgaag ttgccagggt aactacatcc
1021 tgtctttctc aaggaccatc ccagaatgtg gcacccacta gccgttacca tagcaactgc
1081 ctctttgccc cacttaatcc catcccgtct gttaaaaggg ccctatagtt ggaggtgggg
1141 gaggtaggaa gagcgatgat cacttgtgga ctaagtttgt tcgcatcccc ttctccaacc
1201 ccctcagtac atcaccctgg gggaacaggg tccacttgct cctgggccca cacagtcctg
1261 cagtattgtg tatataaggc cagggcaaag aggagcaggt tttaaagtga aaggcaggca
1321 ggtgttgggg aggcagttac cggggcaacg ggaacagggc gtttcggagg tggttgccat
1381 ggggacctgg atgctgacga aggctcgcga ggctgtgagc agccacagtg ccctgctcag
1441 aagccccaag ctcgtcagtc aagccggttc tccgtttgca ctcaggagca cgggcaggcg
1501 agtggcccct agttctgggg gcagcggggg atccgccacc atggtttcta tcgcactgca
1561 ggctggttat gacctcctcg gagatggtag acctgaaatc ctgtggctcg gaattggtac
1621 actgctgata ctgatcggaa ccttctactt cctggttaga gggtggggag tgactaacaa
1681 ggatgcacgg gagtactaca cagtgaccat cctggtcagc ggtattgccg gtgcagctta
1741 tctgttaatg ttctttggta tcggcctcgc agaggtgtca gttggaggag agatgctggt
1801 catctactac gcaagatacg cagagtggct gttctgcaca ccactgctgc tgctggacct
1861 ggcactcctg gccaaagttg atagagtgat catcggcact ctcgttggcg tcgatgctct
1921 gatgattgtc acaggcctga taggagcact gtcacatact gccattgcca gatactcttg
1981 gtggctgttc tcaactatct gtatgatcgt cgttctgtat gtcctggcca cttctctgcg
2041 gtcagccgct aaggagaggg gcccagaggt tgcctcaacc ttcaacatcc tcaccgcact
2101 ggttctggtc ctgtggactg cttaccctat catctggatt atcggcaccg agggtgccgg
2161 agttgtgggc ctgggaatcg aaactctgct gttcatggtg ctggacgtca ccggcaaggt
2221 gggcttcggg tttatcctgc tcaggtcaag agccattctg ggagttacag aagcacctga
2281 accttcagct ggagctgata ccggtgccgc cgaccgcccg gtagtagcag tgagcaaggc
2341 ggccgccaag agcaggatca ccagcgaggg cgagtacatc cccctggacc agatcgacat
2401 caacgtggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
2461 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
2521 ctacggcaag ctgaccctga agttcatttg caccaccggc aagctgcccg tgccctggcc
2581 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
2641 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
2701 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
2761 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
2821 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
2881 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
2941 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
3001 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
3061 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
3121 gttctgctac gagaatgaag tgtaagaatt cgatatcaag cttatcgata atcaacctct
3181 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
3241 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
3301 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
3361 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
3421 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
3481 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
3541 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
3601 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
3661 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
3721 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacccgggcg
3781 gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat
3841 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
3901 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
3961 gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga
4021 taaggatctt cctagagcat ggctacgtag ataagtagca tggcgggtta atcattaact
4081 acaaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
4141 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
4201 agcgagcgcg cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
4261 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
4321 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
4381 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
4441 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
4501 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
4561 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
4621 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
4681 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
4741 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
4801 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
4861 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
4921 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
4981 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
5041 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
5101 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
5161 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
5221 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
5281 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
5341 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
5401 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
5461 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
5521 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
5581 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
5641 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
5701 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
5761 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
5821 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
5881 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
5941 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
6001 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
6061 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
6121 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
6181 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
6241 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
6301 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
6361 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
6421 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
6481 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
6541 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
6601 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
6661 tcgccattca ggctgcgcaa ctgttgggaa gg
//
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