| 质粒名称: | pYK62 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000785 |
| 产品用途: | Mammalian PhCMV-driven constitutive SEAP expression vector containing 8 tandem MS2-box repeats in the 3’UTR (PhCMV-SEAP-(MS2-box)8-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | SEAP-(MS2-box)8 |
| 物种来源: |
synthetic
|
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK62
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK62载体质粒基本信息
pYK62质粒图谱载体图谱和pYK62载体序列质粒序列多克隆位点信息
pYK62质粒载体简介
pYK62质粒序列载体序列
LOCUS pYK62 7403 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7403)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 24, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7403
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 881..917
/label=oYK55-SEAP-F
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
primer_bind 900..927
/label=oDJ273-1
misc_feature 901..2460
/label=SEAP
primer_bind complement(2439..2460)
/label=oDJ273-2
primer_bind complement(2441..2475)
/label=pYK62-SEAP-R
primer_bind complement(2444..2460)
/label=Oscl31-4
primer_bind complement(2444..2460)
/label=oSCL083
polyA_signal 2452..2460
/label=SV40 late polyA
misc_feature 2469..2485
/label=oYK58-ms2-F
misc_feature 2469..2473
/label=New Feature
misc_feature 2469..2473
/label=New Feature
misc_feature 2492..2495
/label=linker
primer_bind complement(2497..2511)
/label=Oscl28-2
primer_bind complement(2498..2511)
/label=8x R
misc_feature 2512..2524
/label=linker
misc_feature 2530..2548
/label=MS2 box
misc_feature 2554..2563
/label=linker
misc_feature 2569..2587
/label=MS2 box
primer_bind complement(2581..2605)
/label=oYK55-SEAP-R
misc_feature 2593..2602
/label=linker
misc_feature 2593..2602
/label=linker
misc_feature 2601..2631
/label=hairpin
misc_feature 2607..2625
/label=MS2 box
misc_feature 2630..2642
/label=linker
misc_feature 2641..2671
/label=hairpin
misc_feature 2647..2665
/label=MS2 box
misc_feature 2675..2680
/label=linker
misc_feature 2689..2704
/label=linker
primer_bind complement(2703..2723)
/label=oYK73_msbox4_R
misc_feature 2731..2743
/label=linker
misc_feature 2749..2767
/label=MS2 box
misc_feature 2773..2782
/label=linker
misc_feature 2788..2806
/label=MS2 box
primer_bind complement(2800..2824)
/label=oYK55-SEAP-R
misc_feature 2812..2821
/label=linker
misc_feature 2812..2821
/label=linker
misc_feature 2820..2850
/label=hairpin
misc_feature 2826..2844
/label=MS2 box
misc_feature 2849..2861
/label=linker
misc_feature 2860..2890
/label=hairpin
misc_feature 2866..2884
/label=MS2 box
misc_feature 2894..2899
/label=linker
misc_feature 2908..2923
/label=linker
terminator 2997..3224
/label=bGH PA
misc_feature 3270..3698
/label=f1 origin
misc_feature 3703..4046
/label=SV40 early promoter and origin
misc_feature 3892..3969
/label=SV40 ORI
CDS 4111..4902
/label=Kan/neoR
misc_feature 5076..5206
/label=SV40 early polyadenylation signal
primer_bind complement(5242..5262)
/label=M13-rev
misc_binding complement(5268..5290)
/label=LacO
promoter complement(5295..5324)
/label=lac
misc_feature complement(5589..6262)
/label=pUC origin
CDS complement(6410..7069)
/label=AmpR
promoter complement(7309..7337)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 atgctgctgc tgctgctgct gctgggcctg aggctacagc tctccctggg catcatccca
961 gttgaggagg agaacccgga cttctggaac cgcgaggcag ccgaggccct gggtgccgcc
1021 aagaagctgc agcctgcaca gacagccgcc aagaacctca tcatcttcct gggcgatggg
1081 atgggggtgt ctacggtgac agctgccagg atcctaaaag ggcagaagaa ggacaaactg
1141 gggcctgaga tacccctggc catggaccgc ttcccatatg tggctctgtc caagacatac
1201 aatgtagaca aacatgtgcc agacagtgga gccacagcca cggcctacct gtgcggggtc
1261 aagggcaact tccagaccat tggcttgagt gcagccgccc gctttaacca gtgcaacacg
1321 acacgcggca acgaggtcat ctccgtgatg aatcgggcca agaaagcagg gaagtcagtg
1381 ggagtggtaa ccaccacacg agtgcagcac gcctcgccag ccggcaccta cgcccacacg
1441 gtgaaccgca actggtactc ggacgccgac gtgcctgcct cggcccgcca ggaggggtgc
1501 caggacatcg ctacgcagct catctccaac atggacattg acgtgatcct aggtggaggc
1561 cgaaagtaca tgtttcgcat gggaacccca gaccctgagt acccagatga ctacagccaa
1621 ggtgggacca ggctggacgg gaagaatctg gtgcaggaat ggctggcgaa gcgccagggt
1681 gcccggtatg tgtggaaccg cactgagctc atgcaggctt ccctggaccc gtctgtgacc
1741 catctcatgg gtctctttga gcctggagac atgaaatacg agatccaccg agactccaca
1801 ctggacccct ccctgatgga gatgacagag gctgccctgc gcctgctgag caggaacccc
1861 cgcggcttct tcctcttcgt ggagggtggt cgcatcgacc atggtcatca tgaaagcagg
1921 gcttaccggg cactgactga gacgatcatg ttcgacgacg ccattgagag ggcgggccag
1981 ctcaccagcg aggaggacac gctgagcctc gtcactgccg accactccca cgtcttctcc
2041 ttcggaggct accccctgcg agggagctcc atcttcgggc tggcccctgg caaggcccgg
2101 gacaggaagg cctacacggt cctcctatac ggaaacggtc caggctatgt gctcaaggac
2161 ggcgcccggc cggatgttac cgagagcgag agcgggagcc ccgagtatcg gcagcagtca
2221 gcagtgcccc tggacgaaga gacccacgca ggcgaggacg tggcggtgtt cgcgcgcggc
2281 ccgcaggcgc acctggttca cggcgtgcag gagcagacct tcatagcgca cgtcatggcc
2341 ttcgccgcct gcctggagcc ctacaccgcc tgcgacctgg cgccccccgc cggcaccacc
2401 gacgccgcgc acccgggtta ctctagagtc ggggcggccg gccgcttcga gcagacatga
2461 aaaccccaag cttggtaccg agctcggatc caacttgaac cggtaagaat tgcaacaaac
2521 aaatcaccac cgtgaggatc acccacggtg gtgaaagcct tagcctagcc gtgaggatca
2581 cccacggtag gcacaagaaa ttctacccgt gaggatcacc cacgggtaga atcacaaaga
2641 ctacagccgt gaggatcacc cacggctgta gactcaaact taaaaaggat atcttcagca
2701 aactgatcgg agacgagtcc gtctcaaatt gcaacaaaca aatcaccacc gtgaggatca
2761 cccacggtgg tgaaagcctt agcctagccg tgaggatcac ccacggtagg cacaagaaat
2821 tctacccgtg aggatcaccc acgggtagaa tcacaaagac tacagccgtg aggatcaccc
2881 acggctgtag actcaaactt aaaaaggata tcttcagcaa actgaattct gcagatatcc
2941 agcacagtgg cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac
3001 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
3061 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
3121 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
3181 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
3241 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
3301 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
3361 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
3421 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
3481 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
3541 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
3601 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
3661 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
3721 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
3781 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
3841 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
3901 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
3961 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
4021 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
4081 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
4141 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
4201 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
4261 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
4321 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
4381 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
4441 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
4501 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
4561 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
4621 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
4681 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
4741 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
4801 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
4861 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
4921 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
4981 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
5041 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
5101 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
5161 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
5221 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
5281 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
5341 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
5401 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
5461 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
5521 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
5581 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
5641 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
5701 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
5761 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
5821 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
5881 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
5941 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
6001 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
6061 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
6121 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
6181 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
6241 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
6301 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
6361 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
6421 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
6481 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
6541 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
6601 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
6661 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
6721 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
6781 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
6841 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
6901 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
6961 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
7021 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
7081 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
7141 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
7201 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
7261 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
7321 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
7381 cccgaaaagt gccacctgac gtc
//
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