TAL1-HA-P2A-NLS-tdTomato-PGK-Puro

LOCUS       TAL1-HA-P2A-NLS-tdTomato-PGK-Puro               8245 bp DNA     circular SYN 16-MAR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   Amane
  TITLE     Direct Submission
  JOURNAL   Exported Aug 15, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          815..823
                     /note="/dnas_title=mAID-mClover in pUC-GW-Amp"
     source          851..859
                     /note="/dnas_title=mAID-mClover in pUC-GW-Amp"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     36..70
                     /label=581-HAL-F
     misc_feature    51..811
                     /label=HA-L
     intron          51..359
                     /label=Intron 4
     primer_bind     51..70
                     /label=TAL1-F
     CDS             360..811
                     /codon_start=1
                     /label=TAL1
                     /translation="PHTKVVRRIFTNSRERWRQQNVNGAFAELRKLIPTHPPDKKLSKN
                     EILRLAMKYINFLAKLLNDQEEEGTQRAKTGKDPVVGAGGGGGGGGGGAPPDDLLQDVL
                     SPNSSCGSSLDGAASPDSYTEEPAPKHTARSLHPAMLPAADGAGPR"
     exon            360..811
                     /label=Exon 5
     primer_bind     complement(797..826)
                     /label=581-new-HAL-R
     CDS             815..823
                     /codon_start=1
                     /label=Linker
                     /translation="GSG"
     CDS             824..850
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             860..916
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(877..896)
                     /label=581-seq-F
     CDS             926..946
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             956..976
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             983..2413
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(1709..1729)
                     /label=tdTomato-R
     primer_bind     complement(2391..2433)
                     /label=579-new-HA-R
     primer_bind     complement(2431..2448)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2437..2661
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(2692..2725)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        2786..3284
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2821..2844)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3217..3236
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3324..3923
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3324..3343)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     complement(3767..3776)
                     /label=581-new-HAL-R
     protein_bind    complement(4480..4513)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    4526..5179
                     /label=HA-R
     3'UTR           4526..5179
     exon            4526..5179
                     /label=Exon 5
     primer_bind     complement(5160..5179)
                     /label=TAL1-R
     primer_bind     complement(5191..5210)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5192..5210)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5217..5234)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5217..5233)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5226..5248)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             5429..6223
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6241..7101
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6459..6478)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7272..7860
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7761..7780
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8014..8031
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8148..8169
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8184..8214
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8222..8238
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agcttggtac cgagctcgtt acatacggtc
       61 cctgtcctca aatatatcac agcccaggga gggagacaag cacagaaaca taagtaatga
      121 tcaagagtga tggaactcca gggattgcgg tatcgtggtg gttggttaca tctgtctgga
      181 ggggttgagg aagttcccat agatgaggaa gcacaggaac ttgaatttga agggtggatg
      241 ttttctaagt aaaaggaaag taggcattct aggcttccca agtttcccaa tgtatggaga
      301 tgcctttggg gaaggaatct caagcccatt ctcctaactc ttgtcctcct aatctccagg
      361 tccccacacc aaagttgtgc ggcgtatctt caccaacagc cgggagcgat ggcggcagca
      421 gaatgtgaac ggggcctttg ccgagctccg caagctgatc cccacacatc ccccggacaa
      481 gaagctcagc aagaatgaga tcctccgcct ggccatgaag tatatcaact tcttggccaa
      541 gctgctcaat gaccaggagg aggagggcac ccagcgggcc aagactggca aggaccctgt
      601 ggtgggggct ggtgggggtg gaggtggggg agggggcggc gcgcccccag atgacctcct
      661 gcaagacgtg ctttccccca actccagctg cggcagctcc ctggatgggg cagccagccc
      721 ggacagctac acggaggagc ccgcgcccaa gcacacggcc cgcagcctcc atcctgccat
      781 gctgcctgcc gccgatggag ccggccctcg gaacggatcc ggttacccat acgacgtacc
      841 agattacgct ggatccggtg cgacgaattt tagtctactg aaacaagcgg gagacgtgga
      901 ggaaaaccct ggacctatcg atggtccaaa aaagaagaga aaggtattcg atggtccaaa
      961 aaagaagaga aaggtattcg atatggtgag caagggcgag gaggtcatca aagagttcat
     1021 gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga
     1081 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
     1141 ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta
     1201 cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa
     1261 gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc
     1321 cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga
     1381 cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc
     1441 ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca
     1501 ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta
     1561 ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
     1621 acagtacgag cgctccgagg gccgccacca cctgttcctg gggcatggca ccggcagcac
     1681 cggcagcggc agctccggca ccgcctcctc cgaggacaac aacatggccg tcatcaaaga
     1741 gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga
     1801 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     1861 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa
     1921 ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg
     1981 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga
     2041 ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc
     2101 ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct
     2161 gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg
     2221 cggccgctac ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc
     2281 cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat
     2341 cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctgtacg gcatggacga
     2401 gctgtacaag taagtcgacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     2461 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     2521 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     2581 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     2641 gggatgcggt gggctctatg ggtctcaacc ttctgaggcg gaaagaacca gataacttcg
     2701 tataatgtat gctatacgaa gttattaggt ctgaagagga gtttacgtcc agccaagctt
     2761 aggatctcga cctcgaaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
     2821 gcatgcgctt tagcagcccc gctggcactt ggcgctacac aagtggcctc tggcctcgca
     2881 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     2941 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     3001 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     3061 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     3121 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     3181 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     3241 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc atccatctag
     3301 atctcgagca gctgaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     3361 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     3421 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     3481 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     3541 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     3601 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     3661 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     3721 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     3781 gcgccggggt gcccgccttc ctggagacct ccgcgcccca caacctcccc ttctacgagc
     3841 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     3901 tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga
     3961 gcgcacgacc ccatgcatcg atgatatcag atccccggga tgcagaaatt gatgatctat
     4021 taaacaataa agatgtccac taaaatggaa gtttttcctg tcatactttg ttaagaaggg
     4081 tgagaacaga gtacctacat tttgaatgga aggattggag ctacgggggt gggggtgggg
     4141 tgggattaga taaatgcctg ctctttactg aaggctcttt actattgctt tatgataatg
     4201 tttcatagtt ggatatcata atttaaacaa gcaaaaccaa attaagggcc agctcattcc
     4261 tcccactcat gatctataga tctatagatc tctcgtggga tcattgtttt tctcttgatt
     4321 cccactttgt ggttctaagt actgtggttt ccaaatgtgt cagtttcata gcctgaagaa
     4381 cgagatcagc agcctctgtt ccacatacac ttcattctca gtattgtttt gccaagttct
     4441 aattccatca gaagctggtc gagatccgga acccttaata taacttcgta taatgtatgc
     4501 tatacgaagt tattaggtcc ctcgatgggt ctgggccacc aggatcagcc aggagggcgt
     4561 tcttaggctg ctgggatggt gggcttcagg gcaggtgggg tgagaattgg gcggctctga
     4621 agcaaggcgg tggacttgaa ctttcctgga tgtctgaact ttgggaagcc tttactgacc
     4681 ctggggctgg cttttctgtt tcctgtacca gtaggagatc agaaaaatgg agcaaagtgg
     4741 taggtacttt ttgtgaagac ggcacggtct tccctcttcc ctcagtccca aatccttccc
     4801 aagtaagagg ctggagttgt cactgctttt ggcctggagt ttgggatccc tgtctttcct
     4861 aagacctggg gttgtcagct ctcatctgag gcatccagca gtctctgcct tgcctttagc
     4921 ccctcccaag ctggctgggg tggcctgtgt ggccacttct gtccatattt ataggtaccc
     4981 aatagctgcc catttcgtga gccccatctt cacccaggcc tatgttgatc catccagctt
     5041 gccagatgct gcagagtcac aagcctcgag gtgccttctt cagggcctgg ttgaagaaga
     5101 tgatcagtgg acagtctgct ctagatgagc tgggccggag ggtcaggaaa cccagtcgcc
     5161 cttacttctt gccctggggg gcccaattcg ccctatagtg agtcgtatta caattcactg
     5221 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     5281 gcagcacatc cccctttcgc cagctggggc gccctctggt aaggttggga agccctgcaa
     5341 agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat caagatctga
     5401 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     5461 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     5521 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     5581 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     5641 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     5701 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tcccaccttg ctcctgccga
     5761 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     5821 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     5881 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     5941 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     6001 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     6061 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     6121 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     6181 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaattgaaa aaggaagagt
     6241 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     6301 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     6361 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     6421 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     6481 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     6541 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     6601 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     6661 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     6721 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     6781 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     6841 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     6901 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     6961 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     7021 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     7081 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     7141 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     7201 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7261 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7321 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7381 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     7441 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7501 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7561 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7621 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7681 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7741 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     7801 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7861 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7921 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     7981 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     8041 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     8101 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     8161 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     8221 attgtgagcg gataacaatt tcaca
//