| 质粒名称: | pAAVLINK-EF1a-HA-5'-CEP290 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001719 |
| 产品用途: | 5' AAVLINK plasmid for CEP290 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CEP290 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-CEP290
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-CEP290载体质粒基本信息
pAAVLINK-EF1a-HA-5'-CEP290质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-CEP290载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-CEP290质粒载体简介
pAAVLINK-EF1a-HA-5'-CEP290质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-CEP290 7349 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7349)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7349
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..4489
/codon_start=1
/label=CEP290
/translation="MPPNINWKEIMKVDPDDLPRQEELADNLLISLSKVEVNELKSEKQ
ENVIHLFRITQSLMKMKAQEVELALEEVEKAGEEQAKFENQLKTKVMKLENELEMAQQS
AGGRDTRFLRNEICQLEKQLEQKDRELEDMEKELEKEKKVNEQLALRNEEAENENSKLR
RENKRLKKKNEQLCQDIIDYQKQIDSQKETLLSRRGEDSDYRSQLSKKNYELIQYLDEI
QTLTEANEKIEVQNQEMRKNLEESVQEMEKMTDEYNRMKAIVHQTDNVIDQLKKENDHY
QLQVQELTDLLKSKNEEDDPIMVAVNAKVEEWKLILSSKDDEIIEYQQMLHNLREKLKN
AQLDADKSNVMALQQGIQERDSQIKMLTEQVEQYTKEMEKNTCIIEDLKNELQRNKGAS
TLSQQTHMKIQSTLDILKEKTKEAERTAELAEADAREKDKELVEALKRLKDYESGVYGL
EDAVVEIKNCKNQIKIRDREIEILTKEINKLELKISDFLDENEALRERVGLEPKTMIDL
TEFRNSKHLKQQQYRAENQILLKEIESLEEERLDLKKKIRQMAQERGKRSATSGLTTED
LNLTENISQGDRISERKLDLLSLKNMSEAQSKNEFLSRELIEKERDLERSRTVIAKFQN
KLKELVEENKQLEEGMKEILQAIKEMQKDPDVKGGETSLIIPSLERLVNAIESKNAEGI
FDASLHLKAQVDQLTGRNEELRQELRESRKEAINYSQQLAKANLKIDHLEKETSLLRQS
EGSNVVFKGIDLPDGIAPSSASIINSQNEYLIHLLQELENKEKKLKNLEDSLEDYNRKF
AVIRHQQSLLYKEYLSEKETWKTESKTIKEEKRKLEDQVQQDAIKVKEYNNLLNALQMD
SDEMKKILAENSRKITVLQVNEKSLIRQYTTLVELERQLRKENEKQKNELLSMEAEVCE
KIGCLQRFKEMAIFKIAALQKVVDNSVSLSELELANKQYNELTAKYRDILQKDNMLVQR
TSNLEHLECENISLKEQVESINKELEITKEKLHTIEQAWEQETKLGNESSMDKAKKSIT
NSDIVSISKKITMLEMKELNERQRAEHCQKMYEHLRTSLKQMEERNFELETKFAELTKI
NLDAQKVEQMLRDELADSVSKAVSDADRQRILELEKNEMELKVEVSKLREISDIARRQV
EILNAQQQSRDKEVESLRMQLLDYQAQSDEKSLIAKLHQHNVSLQLSEATALGKLESIT
SKLQKMEAYNLRLEQKLDEKEQ"
misc_feature 1206..1211
/label=SDS
misc_feature 2752..2757
/label=SDS
intron 4490..4530
/label=Splicing Donor
misc_feature 4534..4567
/label=lox2272-JT1571
repeat_region 4612..4752
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4612..4741
/label=AAV2 ITR
rep_origin 4827..5282
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5564..5668
/gene="bla"
/label=AmpR promoter
CDS 5669..6529
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6700..7288
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat gccacctaat ataaactgga aagaaataat
781 gaaagttgac ccagatgacc tgccccgtca agaagaactg gcagataatt tattgatttc
841 cttatccaag gtggaagtaa atgagctaaa aagtgaaaag caagaaaatg tgatacacct
901 tttcagaatt actcagtcac taatgaagat gaaagctcaa gaagtggagc tggctttgga
961 agaagtagaa aaagctggag aagaacaagc aaaatttgaa aatcaattaa aaactaaagt
1021 aatgaaactg gaaaatgaac tggagatggc tcagcagtct gcaggtggac gagatactcg
1081 gtttttacgt aatgaaattt gccaacttga aaaacaatta gaacaaaaag atagagaatt
1141 ggaggacatg gaaaaggagt tggagaaaga gaagaaagtt aatgagcaat tggctcttcg
1201 aaatgaggag gcagaaaatg aaaacagcaa attaagaaga gagaacaaac gtctaaagaa
1261 aaagaatgaa caactttgtc aggatattat tgactaccag aaacaaatag attcacagaa
1321 agaaacactt ttatcaagaa gaggggaaga cagtgactac cgatcacagt tgtctaaaaa
1381 aaactatgag cttatccaat atcttgatga aattcagact ttaacagaag ctaatgagaa
1441 aattgaagtt cagaatcaag aaatgagaaa aaatttagaa gagtctgtac aggaaatgga
1501 gaagatgact gatgaatata atagaatgaa agctattgtg catcagacag ataatgtaat
1561 agatcagtta aaaaaagaaa acgatcatta tcaacttcaa gtgcaggagc ttacagatct
1621 tctgaaatca aaaaatgaag aagatgatcc aattatggta gctgtcaatg caaaagtaga
1681 agaatggaag ctaattttgt cttctaaaga tgatgaaatt attgagtatc agcaaatgtt
1741 acataaccta agggagaaac ttaagaatgc tcagcttgat gctgataaaa gtaatgttat
1801 ggctctacag cagggtatac aggaacgaga cagtcaaatt aagatgctca ccgaacaagt
1861 agaacaatat acaaaagaaa tggaaaagaa tacttgtatt attgaagatt tgaaaaatga
1921 gctccaaaga aacaaaggtg cttcaaccct ttctcaacag actcatatga aaattcagtc
1981 aacgttagac attttaaaag agaaaactaa agaggctgag agaacagctg aactggctga
2041 ggctgatgct agggaaaagg ataaagaatt agttgaggct ctgaagaggt taaaagatta
2101 tgaatcggga gtatatggtt tagaagatgc tgtcgttgaa ataaagaatt gtaaaaacca
2161 aattaaaata agagatcgag agattgaaat attaacaaag gaaatcaata aacttgaatt
2221 gaagatcagt gatttccttg atgaaaatga ggcacttaga gagcgtgtgg gccttgaacc
2281 aaagacaatg attgatttaa ctgaatttag aaatagcaaa cacttaaaac agcagcagta
2341 cagagctgaa aaccagattc ttttgaaaga gattgaaagt ctagaggaag aacgacttga
2401 tctgaaaaaa aaaattcgtc aaatggctca agaaagagga aaaagaagtg caacttcagg
2461 attaaccact gaggacctga acctaactga aaacatttct caaggagata gaataagtga
2521 aagaaaattg gatttattga gcctcaaaaa tatgagtgaa gcacaatcaa agaatgaatt
2581 tctttcaaga gaactaattg aaaaagaaag agatttagaa aggagtagga cagtgatagc
2641 caaatttcag aataaattaa aagaattagt tgaagaaaat aagcaacttg aagaaggtat
2701 gaaagaaata ttgcaagcaa ttaaggaaat gcagaaagat cctgatgtta aaggaggaga
2761 aacatctcta attatcccta gccttgaaag actagttaat gctatagaat caaagaatgc
2821 agaaggaatc tttgatgcga gtctgcattt gaaagcccaa gttgatcagc ttaccggaag
2881 aaatgaagaa ttaagacagg agctcaggga atctcggaaa gaggctataa attattcaca
2941 gcagttggca aaagctaatt taaagataga ccatcttgaa aaagaaacta gtcttttacg
3001 acaatcagaa ggatcaaatg ttgtttttaa aggaattgac ttacctgatg ggatagcacc
3061 atctagtgcc agtatcatta attctcagaa tgaatattta atacatttgt tacaggaact
3121 agaaaataaa gaaaaaaagt taaagaattt agaagattct cttgaagatt acaacagaaa
3181 atttgctgta attcgtcatc aacaaagttt gttgtataaa gaatacctaa gtgaaaagga
3241 gacctggaaa acagaatcta aaacaataaa agaggaaaag agaaaacttg aggatcaagt
3301 ccaacaagat gctataaaag taaaagaata taataatttg ctcaatgctc ttcagatgga
3361 ttcggatgaa atgaaaaaaa tacttgcaga aaatagtagg aaaattactg ttttgcaagt
3421 gaatgaaaaa tcacttataa ggcaatatac aaccttagta gaattggagc gacaacttag
3481 aaaagaaaat gagaagcaaa agaatgaatt gttgtcaatg gaggctgaag tttgtgaaaa
3541 aattgggtgt ttgcaaagat ttaaggaaat ggccattttc aagattgcag ctctccaaaa
3601 agttgtagat aatagtgttt ctttgtctga actagaactg gctaataaac agtacaatga
3661 actgactgct aagtacaggg acatcttgca aaaagataat atgcttgttc aaagaacaag
3721 taacttggaa cacctggagt gtgaaaacat ctccttaaaa gaacaagtgg agtctataaa
3781 taaagaactg gagattacca aggaaaaact tcacactatt gaacaagcct gggaacagga
3841 aactaaatta ggtaatgaat ctagcatgga taaggcaaag aaatcaataa ccaacagtga
3901 cattgtttcc atttcaaaaa aaataactat gctggaaatg aaggaattaa atgaaaggca
3961 gcgggctgaa cattgtcaaa aaatgtatga acacttacgg acttcgttaa agcaaatgga
4021 ggaacgtaat tttgaattgg aaaccaaatt tgctgagctt accaaaatca atttggatgc
4081 acagaaggtg gaacagatgt taagagatga attagctgat agtgtgagca aggcagtaag
4141 tgatgctgat aggcaacgga ttctagaatt agagaagaat gaaatggaac taaaagttga
4201 agtgtcaaaa ctgagagaga tttctgatat tgccagaaga caagttgaaa ttttgaatgc
4261 acaacaacaa tctagggaca aggaagtaga gtccctcaga atgcaactgc tagactatca
4321 ggcacagtct gatgaaaagt cgctcattgc caagttgcac caacataatg tctctcttca
4381 actgagtgag gctactgctc ttggtaagtt ggagtcaatt acatctaaac tgcagaagat
4441 ggaggcctac aacttgcgct tagagcagaa acttgatgaa aaagaacagg taagtatcaa
4501 ggttacaaga caggtttaag gagaccaata agctattatt cgtataggat actttatacg
4561 aagttattaa gttatggaga ccggtgtggc acgtgcggac cgagcggccg caggaacccc
4621 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
4681 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
4741 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
4801 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
4861 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt
4921 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
4981 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
5041 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
5101 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc
5161 tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct attggttaaa
5221 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
5281 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
5341 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
5401 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
5461 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
5521 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
5581 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
5641 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
5701 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
5761 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
5821 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
5881 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
5941 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
6001 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
6061 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
6121 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
6181 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
6241 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
6301 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
6361 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
6421 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
6481 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
6541 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
6601 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
6661 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
6721 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
6781 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
6841 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
6901 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
6961 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
7021 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
7081 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
7141 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
7201 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
7261 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
7321 ggccttttgc tggccttttg ctcacatgt
//
