| 质粒名称: | hFerritinH |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 122652 |
| 存储实验室: | Trevor Douglas |
|
载体骨架基本信息 |
|
| 载体名称: | pET30a |
| 载体出品公司: | Clontech |
| 空载体大小: | 5826bp |
| 完整质粒大小: | 5826bp |
| 载体类型: | 细菌表达 |
| 载体抗性: | Kanamycin, 50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | ferritin heavy chain 1 |
| 物种来源: | H. sapiens (human) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | unknown (unknown if destroyed) |
| 3’克隆位点: | unknown (unknown if destroyed) |
| 5’测序引物 | NA |
|
文献引用方法 |
|
| 材料和方法部分: |
hFerritinH was a gift from Trevor Douglas (Addgene plasmid # 122652 ; http://n2t.net/addgene:122652 ; RRID:Addgene_122652)
|
买家导航
hFerritinH载体质粒基本信息
hFerritinH质粒图谱载体图谱和hFerritinH载体序列质粒序列多克隆位点信息
hFerritinH质粒载体简介
hFerritinH质粒序列载体序列
LOCUS hFerritinH 5826 bp DNA circular SYN 14-APR-2025
DEFINITION Expression of hFerritinH in bacterial cells.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5826)
TITLE Trevor Douglas lab unpublished plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5826)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT Sequence Label: hFerritinH
FEATURES Location/Qualifiers
source 1..5826
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 973..992
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(1012..1089)
/gene="lacI"
/label=lacI promoter
primer_bind 1295..1314
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind 1402..1421
/label=T7
/note="T7 promoter, forward primer"
promoter 1402..1420
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 1421..1445
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 1460..1482
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 2088..2105
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(2158..2176)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 2172..2219
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 2256..2711
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2343..2362)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2553..2574
/label=F1ori-F
/note="F1 origin, forward primer"
CDS complement(2803..3618)
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
CDS complement(2803..3618)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind 3526..3545
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 3610..3629
/label=pENTR-R
/note="pENTR vectors, reverse primer"
rep_origin 3740..4328
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4229..4248
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 4482..4499
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 4514..4653
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(4579..4598)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4739..4761
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(4755..4946)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
CDS complement(join(5755..5826,1..1011))
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
ORIGIN
1 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
61 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
121 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
181 ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt
241 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
301 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
361 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
421 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
481 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
541 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
601 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
661 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
721 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
781 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
841 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
901 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
961 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
1021 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
1081 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca
1141 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
1201 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
1261 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
1321 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
1381 tcgatctcga tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca
1441 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgacgaccgc
1501 gtccacctcg caggtgcgcc agaactacca ccaggactca gaggccgcca tcaaccgcca
1561 gatcaacctg gagctctacg cctcctacgt ttacctgtcc atgtcttact actttgaccg
1621 cgatgatgtg gctttgaaga actttgccaa atactttctt caccaatctc atgaggagag
1681 ggaacatgct gagaaactga tgaagctgca gaaccaacga ggtggccgaa tcttccttca
1741 ggatatcaag aaaccagact gtgatgactg ggagagcggg ctgaatgcaa tggagtgtgc
1801 attacatttg gaaaaaaatg tgaatcagtc actactggaa ctgcacaaac tggccactga
1861 caaaaatgac ccccatttgt gtgacttcat tgagacacat tacctgaatg agcaggtgaa
1921 agccatcaaa gaattgggtg accacgtgac caacttgcgc aagatgggag cgcccgaatc
1981 tggcttggcg gaatatctct ttgacaagca caccctggga gacagtgata atgaaagcta
2041 aggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc actcgagcac caccaccacc
2101 accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
2161 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
2221 tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag
2281 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
2341 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
2401 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
2461 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
2521 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
2581 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
2641 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
2701 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
2761 ttctaaatac attcaaatat gtatccgctc atgaattaat tcttagaaaa actcatcgag
2821 catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag
2881 ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg
2941 gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc
3001 aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg
3061 caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc
3121 aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa
3181 tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa
3241 cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa
3301 tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa
3361 atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc
3421 tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg
3481 cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt
3541 atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctagagc aagacgtttc
3601 ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag acagttttat
3661 tgttcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
3721 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
3781 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
3841 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
3901 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
3961 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
4021 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
4081 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
4141 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
4201 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
4261 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
4321 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
4381 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
4441 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
4501 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
4561 gcaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
4621 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
4681 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
4741 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg
4801 taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc
4861 agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta
4921 agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg
4981 ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa
5041 catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac
5101 cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca
5161 cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac
5221 ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag
5281 gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc
5341 tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc
5401 atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg
5461 gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc
5521 gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc
5581 gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg
5641 atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc
5701 ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg
5761 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
5821 gggaga
//
