| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-YTHDC2-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001469 |
| 产品用途: | 3' AAVLINK plasmid for YTHDC2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | YTHDC2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-YTHDC2-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-YTHDC2-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-YTHDC2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-YTHDC2-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-YTHDC2-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-YTHDC2-FLAG 6914 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6914)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 11, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6914
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3615
/codon_start=1
/label=YTHDC2
/translation="GDVDLEFVFVCSVDSDSRICWNFRLRNF*ECHYRNFAYIPSC*PQ
LIVPLLIFL*KLLNLHQL*L*EMLYKCLRQ*MQWIHGKI*LNLGIIWLTCQ*NHILVKW
SCVLLF*SVWTPSLQLLAH*LIEILLYYLLRPLKNVQLCFVGNVLLQELSVTIWHFSEH
SRPGKKHEVMGGSEPFVKRIFFHRLLWK*S*A*EHSCLVNLEHQVLLEHEVVVTFGTLT
QTLRIGLSLKLHWWQACILI*STWTERI*C*QGQRRKKYDFILLQFSVSLNIKRFLQPM
VKLQQLRHCPQIGLFMMK*PEPIE*LILDVVQQ*RLSLYWYSVDQLDWQVMLFRNLHPL
EWMAFPMTVVIVKWRTKLQLIWQP*NLMSGSISHWSQRQLVYCCSSDRSGIAYFYAE*E
LHLNLGLKLMKLP*EQL*LF*ALKNSLQVYNNHLGLAKGQGLCLQKSFLWPHLGGQIIV
GKVQQILNFLMSVLLQKEY**NLHLQHYTHLRSTKIEEFYILNEVLRTDQISLL*NLQT
AVVTQVLVLVLLLHPQERAQNLLRQDQTCLFDTS**RVAI*ETLKFLNRRVSGLQLLVM
NGS*IEPFGKAA*FTWYFLFKDLDISRDFLGCLLRLEGKRVRTGALLD*EEYLRWSGYE
KKAFPFNLHTIYSIHGMTTRKCR*AGMGRN*NLRLVNSCSSYGNVFPWEKKTQL"
misc_feature 3420..3425
/label=SDS
CDS 3622..3687
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3703..4134
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3847..3852
/label=SDS
repeat_region 4177..4317
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4177..4306
/label=AAV2 ITR
rep_origin 4392..4847
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5129..5233
/gene="bla"
/label=AmpR promoter
CDS 5234..6094
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6265..6853
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggcg atgtagacct
1561 ggaatttgtt ttcgtctgtt cagtagactc cgattccaga atatgttgga atttcagact
1621 ccggaacttt tgagaatgcc attacaggaa ctttgcttac ataccaagct gttagcccca
1681 gttaattgtc ccattgctga ttttcttatg aaagctcctg aacctccacc agctttaatt
1741 gtaagaaatg ctgtacaaat gcttaagaca atagatgcaa tggatacatg ggaagatctg
1801 actgaacttg ggtatcattt ggctgacttg ccagtagaac cacatcttgg taaaatggtc
1861 ttgtgtgctg ttgttttaaa gtgtctggac cccatcctta caattgcttg cacactagct
1921 tatcgagatc cttttgtact acctactcag gcctctcaaa aacgtgcagc tatgctttgt
1981 aggaaacgtt ttactgcagg agctttcagt gaccatatgg cacttctcag agcattccag
2041 gcctggcaaa aagcacgaag tgatgggtgg gagcgagcct tttgtgaaaa gaattttctt
2101 tcacaggcta ctatggaaat aatcataggc atgagaacac agttgcttgg tcaacttaga
2161 gcatcaggtt ttgttagagc acgaggtggt ggtgacattc gggacgttaa cacaaactct
2221 gagaattggg ctgtcgttaa agctgcattg gtggcaggca tgtatcctaa tttagtccac
2281 gtggacagag agaatctagt gttgacaggg ccaaaggaga aaaaagtacg atttcatcct
2341 gcttcagttc tcagtcagcc tcaatataaa aagattcctc cagccaatgg tcaagctgca
2401 gcaattaagg cactgcccac agattggctt atttatgatg aaatgaccag agcccataga
2461 atagctaata ttagatgttg ttcagcagtg acgcctgtca ctatattggt attctgtgga
2521 ccagctagat tggcaagtaa tgctcttcag gaaccttcat cctttagagt ggatggcatt
2581 cccaatgaca gtagtgatag tgaaatggag gacaaaacta cagctaattt ggcagccttg
2641 aaacttgatg agtggctcca tttcacactg gagccagagg cagctagttt attgctgcag
2701 ctcagacaga agtggcatag cttattttta cgccgaatga gagctccatc taaaccttgg
2761 tctcaagttg atgaagctac cataagagca attatagctg ttttaagcac tgaagaacag
2821 tctgcaggtt tacaacaacc atctgggatt ggccaaaggc caaggcctat gtcttcagaa
2881 gagcttcctt tggcctcatc ttggaggtca aataatagta ggaaaagttc agcagatact
2941 gaattttctg atgagtgtac tactgcagaa agagtactga tgaaatctcc atctccagca
3001 ttacacccac ctcagaagta caaagataga ggaattttac atcctaaacg aggtactgag
3061 gaccgatcag atcagtcttc tctgaaatct acagacagca gtagttaccc aagtccttgt
3121 gctagtcctt ctcctccatc ctcaggaaag ggctcaaaat ctccttcgcc aagaccaaac
3181 atgcctgttc gatacttcat aatgaagagt agcaatttga gaaaccttga aatttctcaa
3241 cagaagggta tctggtctac aactcctagt aatgaacgga agctaaatcg agccttttgg
3301 gaaagcagca tagtttactt ggtattttct gttcaaggat ctggacattt ccagggattt
3361 tctaggatgt cttctgagat tggaagggaa aagagtcagg actggggctc tgctggacta
3421 ggaggagtat ttaaggtgga gtggatacga aaagaaagcc ttccctttca atttgcacac
3481 catttactca atccatggaa tgacaacaag aaagtgcaga taagcaggga tgggcaggaa
3541 ctagaacctc aggttggtga acagttgctc cagttatggg aacgtcttcc cttgggagaa
3601 aaaaacacaa ctgattgtac agactacaaa gaccatgacg gtgattataa agatcatgac
3661 atcgactaca aggatgacga tgacaagtaa gaattctgat gactcgagat aatcaacctc
3721 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
3781 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
3841 ttttctcctc cttgtataaa tcctggttag ttcttgccac ggcggaactc atcgccgcct
3901 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttta
3961 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat ctagctttat ttgtgaaatt
4021 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
4081 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagatct
4141 ggagaccggt gtggcacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
4201 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
4261 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
4321 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
4381 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
4441 cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc
4501 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
4561 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
4621 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
4681 ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc
4741 ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta
4801 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
4861 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
4921 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
4981 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
5041 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
5101 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
5161 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
5221 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
5281 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
5341 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
5401 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
5461 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
5521 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
5581 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
5641 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
5701 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
5761 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
5821 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
5881 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
5941 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
6001 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
6061 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
6121 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
6181 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
6241 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
6301 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
6361 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
6421 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
6481 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
6541 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
6601 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
6661 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
6721 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
6781 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
6841 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
6901 ttttgctcac atgt
//
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