| 出品公司: | -- |
|---|---|
| 载体名称: | pTRKH3-ldhGFP |
| 质粒类型: | 乳酸菌表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | -- |
| 载体大小: | 8509 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 红霉素 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pTRKH3-ldhGFP 8509 bp DNA circular SYN 12-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8509)
AUTHORS Biofeng
TITLE Direct Submission
JOURNAL Exported Jul 12, 2023 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8509
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter complement(5843..5945)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
rep_origin complement(6471..7016)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
promoter 7128..7156
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
CDS complement(7499..8215)
/codon_start=1
/gene="mgfp5"
/product="GFP with folding enhancement mutations"
/label=mgfp5
/note="suitable for expression in plants due to removal of
a cryptic intron"
/translation="MSKGEELFTGVVPILVELDGDVNGYKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
NFKTRHNIEDGGVQLADHYQQNTPIGDDPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
ORIGIN
1 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat
61 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
121 ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg
181 cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc
241 cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct
301 gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct
361 tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga
421 tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat
481 cactggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt
541 ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc
601 atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc
661 actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct
721 tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgagt
781 ctagaatcga tacgattttg aagtggcaac agataaaaaa aagcagttta aaattgttgc
841 tgaactttta aaacaagcaa atacaatcat tgtcgcaaca gatagcgaca gagaaggcga
901 aaacattgcc tggtcgatca ttcataaagc aaatgccttt tctaaagata aaacgtataa
961 aagactatgg atcaatagtt tagaaaaaga tgtgatccgt agcggttttc aaaatttgca
1021 accaggaatg aattactatc ccttttatca agaagcgcac aaaaagaaaa acgaaatgat
1081 acaccaatca gtgcaaaaaa agatataatg ggagataaga cggttcgtgt tcgtgctgac
1141 ttgcaccata tcataaaaat cgaaacagca aagaatggcg gaaacgtaaa agaagttatg
1201 gaaataagac ttagaagcaa acttaagagt gtgttgatag tgcagtatct taaaattttg
1261 tataatagga attgaagtta aattagatgc taaaaatttg taattaagaa ggagtgatta
1321 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
1381 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
1441 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
1501 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
1561 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggag
1621 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc
1681 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
1741 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
1801 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
1861 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
1921 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
1981 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
2041 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
2101 ctaaagggaa tgtagataaa ttattaggta tactactgac agcttccaag gagctaaaga
2161 ggtccctagc gctcttatca tggggaagct cggatcatat gcaagacaaa ataaactcgc
2221 aacagcactt ggagaaatgg gacgaatcga gaaaaccctc tttacgctgg attacatatc
2281 taataaagcc gtaaggagac gggttcaaaa aggtttaaat aaaggagaag caatcaatgc
2341 attagctaga actatatttt ttggacaacg tggagaattt agagaacgtg ctctccaaga
2401 ccagttacaa agagctagtg cactaaacat aattattaac gctataagtg tgtggaacac
2461 tgtatatatg gaaaaagccg tagaagaatt aaaagcaaga ggagaattta gagaagattt
2521 aatgccatat gcgtggccgt taggatggga acatatcaat tttcttggag aatacaaatt
2581 tgaaggatta catgacactg ggcaaatgaa tttacgtcct ttacgtataa aagagccgtt
2641 ttattcttaa tataacggct ctttttatag aaaaaatcct tagcgtggtt tttttccgaa
2701 atgctggcgg taccccaaga attagaaatg agtagatcaa attattcacg aatagaatca
2761 ggaaaatcag atccaaccat aaaaacacta gaacaaattg caaagttaac taactcaacg
2821 ctagtagtgg atttaatccc aaatgagcca acagaaccag agccagaaac agaatcagaa
2881 caagtaacat tggatttaga aatggaagaa gaaaaaagca atgacttcgt gtgaataatg
2941 cacgaaatcg ttgcttattt ttttttaaaa gcggtatact agatataacg aaacaacgaa
3001 ctgaatagaa acgaaaaaag agccatgaca catttataaa atgtttgacg acattttata
3061 aatgcatagc ccgataagat tgccaaacca acgcttatca gttagtcaga tgaactcttc
3121 cctcgtaaga agttatttaa ttaactttgt ttgaagacgg tatataaccg tactatcatt
3181 atatagggaa atcagagagt tttcaagtat ctaagctact gaatttaaga attgttaagc
3241 aatcaatcgg aaatcgtttg attgcttttt ttgtattcat ttatagaagg tggagtttgt
3301 atgaatcatg atgaatgtaa aacttatata aaaaatagtt tattggagat aagaaaatta
3361 gcaaatatct atacactaga aacgtttaag aaagagttag aaaagagaaa tatctactta
3421 gaaacaaaat cagataagta tttttcttcg gagggggaag attatatata taagttaata
3481 gaaaataaca aaataattta ttcgattagt ggaaaaaaat tgacttataa aggaaaaaaa
3541 tctttttcaa aacatgcaat attgaaacag ttgaatgaaa aagcaaacca agttaattaa
3601 acaacctatt ttataggatt tataggaaag gagaacagct gaatgaatat cccttttgtt
3661 gtagaaactg tgcttcatga cggcttgtta aagtacaaat ttaaaaatag taaaattcgc
3721 tcaatcacta ccaagccagg taaaagcaaa ggggctattt ttgcgtatcg ctcaaaatca
3781 agcatgattg gcggtcgtgg tgttgttctg acttccgagg aagcgattca agaaaatcaa
3841 gatacattta cacattggac acccaacgtt tatcgttatg gaacgtatgc agacgaaaac
3901 cgttcataca cgaaaggaca ttctgaaaac aatttaagac aaatcaatac cttctttatt
3961 gattttgata ttcacacggc aaaagaaact atttcagcaa gcgatatttt aacaaccgct
4021 attgatttag gttttatgcc tactatgatt atcaaatctg ataaaggtta tcaagcatat
4081 tttgttttag aaacgccagt ctatgtgact tcaaaatcag aatttaaatc tgtcaaagca
4141 gccaaaataa tttcgcaaaa tatccgagaa tattttggaa agtctttgcc agttgatcta
4201 acgtgtaatc attttggtat tgctcgcata ccaagaacgg acaatgtaga attttttgat
4261 cctaattacc gttattcttt caaagaatgg caagattggt ctttcaaaca aacagataat
4321 aagggcttta ctcgttcaag tctaacggtt ttaagcggta cagaaggcaa aaaacaagta
4381 gatgaaccct ggtttaatct cttattgcac gaaacgaaat tttcaggaga aaagggttta
4441 atagggcgta ataacgtcat gtttaccctc tctttagcct actttagttc aggctattca
4501 atcgaaacgt gcgaatataa tatgtttgag tttaataatc gattagatca acccttagaa
4561 gaaaaagaag taatcaaaat tgttagaagt gcctattcag aaaactatca aggggctaat
4621 agggaataca ttaccattct ttgcaaagct tgggtatcaa gtgatttaac cagtaaagat
4681 ttatttgtcc gtcaagggtg gtttaaattc aagaaaaaaa gaagcgaacg tcaacgtgtt
4741 catttgtcag aatggaaaga agatttaatg gcttatatta gcgaaaaaag cgatgtatac
4801 aagccttatt tagtgacgac caaaaaagag attagagaag tgctaggcat tcctgaacgg
4861 acattagata aattgctgaa ggtactgaag gcgaatcagg aaattttctt taagattaaa
4921 ccaggaagaa atggtggcat tcaacttgct agtgttaaat cattgttgct atcgatcatt
4981 aaagtaaaaa aagaagaaaa agaaagctat ataaaggcgc tgacaaattc ttttgactta
5041 gagcatacat tcattcaaga gactttaaac aagctagcag aacgccctaa aacggacaca
5101 caactcgatt tgtttagcta tgatacaggc tgaaaataaa acccgcacta tgccattaca
5161 tttatatcta tgatacgtgt ttgttttttc tttgctgttt agcgaatgat tagcagaaat
5221 atacagagta agattttaat taattattag ggggagaagg agagagtagc ccgaaaactt
5281 ttagttggct tggactgaac gaagtgaggg aaaggctact aaaacgtcga ggggcagtga
5341 gagcgaagcg aacacttgat tttttaattt tctatctttt ataggtcatt agagtatact
5401 tatttgtcct ataaactatt tagcagcata atagatttat tgaataggtc atttaagttg
5461 agcatattag aggaggaaaa tcttggagaa atatttgaag aacccgatta catggattgg
5521 attagttctt gtggttacgt ggtttttaac taaaagtagt gaatttttga tttttggtgt
5581 gtgtgtcttg ttgttagtat ttgctagtca aagtgattaa atagaattcc ggatgagcat
5641 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
5701 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
5761 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
5821 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
5881 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
5941 gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac
6001 caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg
6061 cgtcgggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg aggtgctcca
6121 gtggcttctg tttctatcag ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac
6181 ggcaaaagca ccgccggaca tcagcgctag cggagtgtat actggcttac tatgttggca
6241 ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg
6301 tcagcagaat atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc
6361 ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga
6421 tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg
6481 ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg
6541 acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt
6601 cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg
6661 cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc
6721 cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag
6781 acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga
6841 agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa
6901 gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca
6961 aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag
7021 atcatcttat taatcagata aaatatttct agatttcagt gcaatttatc tcttcaaatg
7081 tagcacctga agtcagcccc atacgatata agttgtaatt ctcatgtttg acagcttatc
7141 atcgataagc tttaatgcgg tagtttatca cagttaaatt gctaacgcag tcaggcaccg
7201 tgtatgaaat ctaacaatgc gctcatcgtc atcctcggca ccgtcaccct ggatgctgta
7261 ggcataggct tggttatgcc ggtactgccg ggcctcttgc gggatatcgt ccattccgac
7321 agcatcgcca gtcactatgg cgtgctgcta gcgctatatg cgttgatgca atttctatgc
7381 gcacccgttc tcggagcact gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg
7441 ctacttggag ccactatcga ctacgcgatc atggcgacca cacccgtcct gtggatcctt
7501 atttgtatag ttcatccatg ccatgtgtaa tcccagcagc cgttacaaac tcaagaagga
7561 ccatgtggtc tctcttttcg ttgggatctt tcgaaagggc agattgtgtg gacaggtaat
7621 ggttgtctgg taaaaggaca gggtcatcgc caattggagt attttgttga taatgatcag
7681 cgagttgcac gccgccgtct tcgatgttgt ggcgggtctt gaagttggct ttgatgccgt
7741 tcttttgctt gtcggccatg atgtatacgt tgtgggagtt gtagttgtat tccaacttgt
7801 ggccgaggat gtttccgtcc tccttgaaat cgattccctt aagctcgatc ctgttgacga
7861 gggtgtctcc ctcaaacttg acttcagcac gtgtcttgta gttcccgtcg tccttgaaga
7921 agatggtcct ctcctgcacg tatccctcag gcatggcgct cttgaagaag tcgtgccgct
7981 tcatatgatc tggatatctt gaaaagcatt gaacaccata agtgagagta gtgacaagtg
8041 ttggccatgg aacaggtagt tttccagtag tgcaaataaa tttaagggta agttttccgt
8101 atgttgcatc accttcaccc tctccactga cagaaaattt gtacccatta acatcaccat
8161 ctaattcaac aagaattggg acaactccag tgaaaagttc ttctccttta ctcatgaatt
8221 caagtctcct tttttattag tgataatttt aacaaagaaa attataccat gttgaagagc
8281 attaataaaa ttattatttt gtgtttgtgc tattatagtt gagattatta ttaatgaggg
8341 gtaaataaga tgaagataat tgcaggtttg ggtaatccgg gtcaaaaata tgataagacc
8401 aaacataata ctggtttcat gacaatggat cactaccttg ataaaaaagg tttgacttta
8461 aataaagata aatttgaagg gcattggact aaaaagctta tcgataccg
//
