| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SCN4A-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001571 |
| 产品用途: | 3' AAVLINK plasmid for SCN4A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SCN4A |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SCN4A-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SCN4A-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SCN4A-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SCN4A-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SCN4A-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SCN4A-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SCN4A-FLAG 7071 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7071)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 18, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7071
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3784
/codon_start=1
/label=SCN4A
/translation="VYFTNAWCWLDFLIVDVSIISLVANWLGYSELGPIKSLRTLRALR
PLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCINTTT
SERFDISEVNNKSECESLMHTGQVRWLNVKVNYDNVGLGYLSLLQVATFKGWMDIMYAA
VDSREKEEQPQYEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGKDIFM
TEEQKKYYNAMKKLGSKKPQKPIPRPQNKIQGMVYDLVTKQAFDITIMILICLNMVTMM
VETDNQSQLKVDILYNINMIFIIIFTGECVLKMLALRQYYFTVGWNIFDFVVVILSIVG
LALSDLIQKYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLL
FLVMFIYSIFGMSNFAYVKKESGIDDMFNFETFGNSIICLFEITTSAGWDGLLNPILNS
GPPDCDPNLENPGTSVKGDCGNPSIGICFFCSYIIISFLIVVNMYIAIILENFNVATEE
SSEPLGEDDFEMFYETWEKFDPDATQFIAYSRLSDFVDTLQEPLRIAKPNKIKLITLDL
PMVPGDKIHCLDILFALTKEVLGDSGEMDALKQTMEEKFMAANPSKVSYEPITTTLKRK
HEEVCAIKIQRAYRRHLLQRSMKQASYMYRHSHDGSGDDAPEKEGLLANTMSKMYGHEN
GNSSSPSPEEKGEAGDAGPTMGLMPISPSDTAWPPAPPPGQTVRPGVKESLV"
misc_feature 1732..1737
/label=SDS
misc_feature 1984..1989
/label=SDS
misc_feature 2052..2057
/label=SDS
misc_feature 2734..2739
/label=SDS
misc_feature 2737..2742
/label=SDS
misc_feature 3456..3461
/label=SDS
misc_feature 3579..3584
/label=SDS
CDS 3791..3856
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3860..4291
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4004..4009
/label=SDS
repeat_region 4334..4474
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4334..4463
/label=AAV2 ITR
rep_origin 4549..5004
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5286..5390
/gene="bla"
/label=AmpR promoter
CDS 5391..6251
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6422..7010
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt acttcaccaa
1561 cgcctggtgc tggctcgact tcctcatcgt ggatgtctcc atcatcagct tggtggccaa
1621 ctggctgggc tactcggagc tgggacccat caaatccctg cggacactgc gggccctgcg
1681 tcccctgagg gcactgtccc gattcgaggg catgagggtg gtggtgaacg ccctcctagg
1741 cgccatcccc tccatcatga atgtgctgct tgtctgcctc atcttctggc tgatcttcag
1801 catcatgggt gtcaacctgt ttgccggcaa gttctactac tgcatcaaca ccaccacctc
1861 tgagaggttc gacatctccg aggtcaacaa caagtctgag tgcgagagcc tcatgcacac
1921 aggccaggtc cgctggctca atgtcaaggt caactacgac aacgtgggtc tgggctacct
1981 ctccctcctg caggtggcca ccttcaaggg ttggatggac atcatgtatg cagccgtgga
2041 ctcccgggag aaggaggagc agccgcagta cgaggtgaac ctctacatgt acctctactt
2101 tgtcatcttc atcatctttg gctccttctt caccctcaac ctcttcattg gcgtcatcat
2161 tgacaacttc aaccagcaga agaagaagtt aggggggaaa gacatcttta tgacggagga
2221 acagaagaaa tactataacg ccatgaagaa gcttggctcc aagaagcctc agaagccaat
2281 tccccggccc cagaacaaga tccagggcat ggtgtatgac ctcgtgacga agcaggcctt
2341 cgacatcacc atcatgatcc tcatctgcct caacatggtc accatgatgg tggagacaga
2401 caaccagagc cagctcaagg tggacatcct gtacaacatc aacatgatct tcatcatcat
2461 cttcacaggg gagtgcgtgc tcaagatgct cgccctgcgc cagtactact tcaccgttgg
2521 ctggaacatc tttgacttcg tggtcgtcat cctgtccatt gtgggccttg ccctctctga
2581 cctgatccag aagtacttcg tgtcacccac gctgttccgt gtgatccgcc tggcgcggat
2641 tgggcgtgtc ctgcggctga tccgcggggc caagggcatc cggacgctgc tgttcgccct
2701 catgatgtcg ctgcctgccc tcttcaacat cggcctcctc ctcttcctgg tcatgttcat
2761 ctactccatc ttcggcatgt ccaactttgc ctacgtcaag aaggagtcgg gcatcgatga
2821 tatgttcaac ttcgagacct tcggcaacag catcatctgc ctgttcgaga tcaccacgtc
2881 ggccggctgg gacgggctcc tcaaccccat cctcaacagc gggcccccag actgtgaccc
2941 caacctggag aacccgggca ccagtgtcaa gggtgactgc ggcaacccct ccatcggcat
3001 ctgcttcttc tgcagctata tcatcatctc cttcctcatc gtggtcaaca tgtacatcgc
3061 catcatcctg gagaacttca atgtggccac agaggagagc agcgagcccc ttggtgaaga
3121 tgactttgag atgttctacg agacatggga gaagttcgac cccgacgcca cccagttcat
3181 cgcctacagc cgcctctcag acttcgtgga caccctgcag gaaccgctga ggattgccaa
3241 gcccaacaag atcaagctca tcacactgga cttgcccatg gtgccagggg acaagatcca
3301 ctgcctggac atcctctttg ccctgaccaa agaggtcctg ggtgactctg gggaaatgga
3361 cgccctcaag cagaccatgg aggagaagtt catggcagcc aacccctcca aggtgtccta
3421 cgagcccatc accaccaccc tcaagaggaa gcacgaggag gtgtgcgcca tcaagatcca
3481 gagggcctac cgccggcacc tgctacagcg ctccatgaag caggcatcct acatgtaccg
3541 ccacagccac gacggcagcg gggatgacgc ccctgagaag gaggggctgc ttgccaacac
3601 catgagcaag atgtatggcc acgagaatgg gaacagcagc tcgccaagcc cggaggagaa
3661 gggcgaggca ggggacgccg gacccactat ggggctgatg cccatcagcc cctcagacac
3721 tgcctggcct cccgcccctc ccccagggca gactgtgcgc ccaggtgtca aggagtctct
3781 tgtctgtaca gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa
3841 ggatgacgat gacaagtaac tcgagataat caacctctgg attacaaaat ttgtgaaaga
3901 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
3961 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
4021 tggttagttc ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
4081 gctcggctgt tgggcactga caattccgtg gtgtttattt gtgaaatttg tgatgctatt
4141 gctttatttg taaccatcta gctttatttg tgaaatttgt gatgctattg ctttatttgt
4201 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
4261 ggttcagggg gagatgtggg aggtttttta aagatctgga gaccggtgtg gcacgtgcgg
4321 accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
4381 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
4441 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt
4501 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
4561 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
4621 gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
4681 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
4741 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
4801 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
4861 aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc
4921 cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
4981 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
5041 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
5101 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
5161 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
5221 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
5281 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
5341 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
5401 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
5461 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
5521 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
5581 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
5641 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
5701 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
5761 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
5821 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
5881 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
5941 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
6001 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
6061 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
6121 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
6181 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
6241 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
6301 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
6361 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
6421 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
6481 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
6541 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
6601 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
6661 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
6721 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
6781 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
6841 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
6901 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
6961 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
7021 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
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pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SCN4A-FLAG质粒载体应用举例
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