pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PTCH1-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PTCH1-FLAG               6924 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 22, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3637
                     /codon_start=1
                     /label=PTCH1
                     /translation="VVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQF
                     KYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQG
                     LQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGI
                     INPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYA
                     QFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLF
                     ISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASV
                     GIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIV
                     RYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFA
                     MPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHS
                     TVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEIS
                     TEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPP
                     VPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGS
                     SSN"
     misc_feature    3560..3565
                     /label=SDS
     CDS             3644..3709
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3713..4144
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3857..3862
                     /label=SDS
     repeat_region   4187..4327
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4187..4316
                     /label=AAV2 ITR
     rep_origin      4402..4857
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5139..5243
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5244..6104
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6275..6863
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtag tggtgatctt
     1561 cctttttctg ggcttgctgg gggtcagcct ttatggcacc acccgagtga gagacgggct
     1621 ggaccttacg gacattgtac ctcgggaaac cagagaatat gactttattg ctgcacaatt
     1681 caaatacttt tctttctaca acatgtatat agtcacccag aaagcagact acccgaatat
     1741 ccagcactta ctttacgacc tacacaggag tttcagtaac gtgaagtatg tcatgttgga
     1801 agaaaacaaa cagcttccca aaatgtggct gcactacttc agagactggc ttcagggact
     1861 tcaggatgca tttgacagtg actgggaaac cgggaaaatc atgccaaaca attacaagaa
     1921 tggatcagac gatggagtcc ttgcctacaa actcctggtg caaaccggca gccgcgataa
     1981 gcccatcgac atcagccagt tgactaaaca gcgtctggtg gatgcagatg gcatcattaa
     2041 tcccagcgct ttctacatct acctgacggc ttgggtcagc aacgaccccg tcgcgtatgc
     2101 tgcctcccag gccaacatcc ggccacaccg accagaatgg gtccacgaca aagccgacta
     2161 catgcctgaa acaaggctga gaatcccggc agcagagccc atcgagtatg cccagttccc
     2221 tttctacctc aacggcttgc gggacacctc agactttgtg gaggcaattg aaaaagtaag
     2281 gaccatctgc agcaactata cgagcctggg gctgtccagt taccccaacg gctacccctt
     2341 cctcttctgg gagcagtaca tcggcctccg ccactggctg ctgctgttca tcagcgtggt
     2401 gttggcctgc acattcctcg tgtgcgctgt cttccttctg aacccctgga cggccgggat
     2461 cattgtgatg gtcctggcgc tgatgacggt cgagctgttc ggcatgatgg gcctcatcgg
     2521 aatcaagctc agtgccgtgc ccgtggtcat cctgatcgct tctgttggca taggagtgga
     2581 gttcaccgtt cacgttgctt tggcctttct gacggccatc ggcgacaaga accgcagggc
     2641 tgtgcttgcc ctggagcaca tgtttgcacc cgtcctggat ggcgccgtgt ccactctgct
     2701 gggagtgctg atgctggcgg gatctgagtt cgacttcatt gtcaggtatt tctttgctgt
     2761 gctggcgatc ctcaccatcc tcggcgttct caatgggctg gttttgcttc ccgtgctttt
     2821 gtctttcttt ggaccatatc ctgaggtgtc tccagccaac ggcttgaacc gcctgcccac
     2881 accctcccct gagccacccc ccagcgtggt ccgcttcgcc atgccgcccg gccacacgca
     2941 cagcgggtct gattcctccg actcggagta tagttcccag acgacagtgt caggcctcag
     3001 cgaggagctt cggcactacg aggcccagca gggcgcggga ggccctgccc accaagtgat
     3061 cgtggaagcc acagaaaacc ccgtcttcgc ccactccact gtggtccatc ccgaatccag
     3121 gcatcaccca ccctcgaacc cgagacagca gccccacctg gactcagggt ccctgcctcc
     3181 cggacggcaa ggccagcagc cccgcaggga cccccccaga gaaggcttgt ggccaccccc
     3241 ctacagaccg cgcagagacg cttttgaaat ttctactgaa gggcattctg gccctagcaa
     3301 tagggcccgc tggggccctc gcggggcccg ttctcacaac cctcggaacc cagcgtccac
     3361 tgccatgggc agctccgtgc ccggctactg ccagcccatc accactgtga cggcttctgc
     3421 ctccgtgact gtcgccgtgc acccgccgcc tgtccctggg cctgggcgga acccccgagg
     3481 gggactctgc ccaggctacc ctgagactga ccacggcctg tttgaggacc cccacgtgcc
     3541 tttccacgtc cggtgtgaga ggagggattc gaaggtggaa gtcattgagc tgcaggacgt
     3601 ggaatgcgag gagaggcccc ggggaagcag ctccaactgt acagactaca aagaccatga
     3661 cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagt aactcgagat
     3721 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     3781 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     3841 atggctttca ttttctcctc cttgtataaa tcctggttag ttcttgccac ggcggaactc
     3901 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     3961 gtggtgttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat ctagctttat
     4021 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     4081 taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt
     4141 ttaaagatct ggagaccggt gtggcacgtg cggaccgagc ggccgcagga acccctagtg
     4201 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
     4261 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc
     4321 ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     4381 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4441 tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt
     4501 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     4561 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
     4621 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4681 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct
     4741 cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg
     4801 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
     4861 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     4921 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     4981 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     5041 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     5101 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5161 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5221 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5281 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5341 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5401 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5461 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5521 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5581 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5641 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     5701 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5761 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     5821 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     5881 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     5941 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6001 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6061 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6121 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6181 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6241 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6301 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6361 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6421 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6481 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6541 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6601 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6661 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6721 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6781 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6841 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6901 tttgctggcc ttttgctcac atgt
//