pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PCM1-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PCM1-FLAG               7256 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7256)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3969
                     /codon_start=1
                     /label=PCM1
                     /translation="VK*RNHLSRLDFQCL*KNLQVVTAKIN*IQTEEDASLMKNHWKAL
                     AVCLIQ*IQQQ*LKHSRQEKRLHRPAWHLKIKLPSQKVRRGILLS*KAELKTSGMKVPV
                     CLAHVNLAKVGTDIQPRLKSLFKQKYSAERIMSNWKK**NVIGLQKYLQKLGVIFPCLK
                     LCEILFILK*LH*FLKMNLVHIFLLNSSMSCSY*TQTT*DRGLYMHCRT*YPDIFLRAM
                     KKEKM*SQ*TLVLG*HQTQNLLLVRALLLLMMKLLRRTLKEKPIK*VSKMMLIMLVSCL
                     YHQILSLLQQMI*VTP*FT*IKH*PE*ENMSV*RLRLKVTQI*DAPAGLLRMEMVLVQV
                     LQLII*KKLPLLKIVVHNNL*VKFLPSHVLELILSSWTGKLKQL*KKSFLF*RSTWMKY
                     APRSF*LQ*GAWF*PLPSKMMRAKSL*SSFINNLEVYYRIHWQNLLAEN*KTVEKIFL*
                     RYLKCCSMNWLSLSLCKIWIIIV*LLNRDAKGK*KQLE*YNLVPKRLKGFLKIMAHLLE
                     RLMMKTKTRMKLKQLSRLKHLRCMMVPKM*DLIFLIKRKMKKVKDVQCLLICLKLKLRL
                     *LIMEVEKMKMRMKKWKNLKKALWMSRLPSRLTLKLLKKMNMMNRSYNVTLKRQQKAKM
                     SHWNEKPLVKMTKITVL*NPVTSISWKMSNL*IVLPIRSHLLLLIQLNSLTLCRYVYLK
                     WNP*CLESKKLNLLRKLLKALWLEVLILNLQC**MTMKQNLVI*VKSLMKKIL*KLKIY
                     H*N*QYIQRQI*ERKW*KKNRKTIYLVKYVKCRPKN*LEILRH*KNLKRWEPRV"
     misc_feature    3571..3576
                     /label=SDS
     CDS             3976..4041
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4045..4476
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4189..4194
                     /label=SDS
     repeat_region   4519..4659
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4519..4648
                     /label=AAV2 ITR
     rep_origin      4734..5189
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5471..5575
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5576..6436
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6607..7195
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtga agtagagaaa
     1561 ccatttatca agactggatt ttcagtgtct gtagaaaaat ctacaagtag taaccgcaaa
     1621 aatcaattag atacaaacgg aagaagacgc cagtttgatg aagaatcact ggaaagcttt
     1681 agcagtatgc ctgatccagt agatccaaca acagtgacta aaacattcaa gacaagaaaa
     1741 gcgtctgcac aggccagcct ggcatctaaa gataaaactc ccaagtcaaa aagtaagaag
     1801 aggaattcta ctcagctgaa aagcagagtt aaaaacatca ggtatgaaag tgccagtatg
     1861 tctagcacat gtgaaccttg caaaagtagg aacagacatt cagcccagac tgaagagcct
     1921 gttcaagcaa aagtattcag cagaaagaat catgagcaac tggaaaaaat aataaaatgt
     1981 aataggtcta cagaaatatc ttcagaaact gggagtgatt tttccatgtt tgaagctttg
     2041 cgagatacta tttattctga agtagctaca ttaatttctc aaaatgaatc tcgtccacat
     2101 tttcttattg aactcttcca tgagctgcag ctactaaaca cagactactt gagacagagg
     2161 gctttatatg cattgcagga catagtatcc agacatattt ctgagagcca tgaaaaagga
     2221 gaaaatgtaa agtcagtaaa ctctggtact tggatagcat caaactcaga acttactcct
     2281 agtgagagcc ttgctactac tgatgatgaa acttttgaga agaactttga aagagaaacc
     2341 cataaaataa gtgagcaaaa tgatgctgat aatgctagtg tcctgtctgt atcatcaaat
     2401 tttgagcctt ttgcaacaga tgatctaggt aacaccgtga ttcacttaga tcaagcatta
     2461 gccagaatga gagaatatga gcgtatgaag actgaggctg aaagtaactc aaatatgaga
     2521 tgcacctgca ggattattga ggatggagat ggtgctggtg caggtactac agttaataat
     2581 ttagaagaaa ctcccgttat tgaaaatcgt agttcacaac aacctgtaag tgaagtttct
     2641 accatcccat gtcctagaat tgatactcag cagctggacc ggcaaattaa agcaattatg
     2701 aaagaagtca ttcctttttt gaaggagcac atggatgaag tatgctcctc gcagcttcta
     2761 acttcagtaa ggcgcatggt tttgaccctt acccagcaaa atgatgagag caaagagttt
     2821 gtaaagttct ttcataaaca acttggaagt atattacagg attcactggc aaaatttgct
     2881 ggcagaaaac tgaaagactg tggagaagat cttcttgtag agatatctga agtgttgttc
     2941 aatgaattgg ctttctttaa gcttatgcaa gatttggata ataatagtat aactgttaaa
     3001 cagagatgca aaaggaaaat agaagcaact ggagtgatac aatcttgtgc caaagaggct
     3061 aaaaggattc ttgaagatca tggctcacct gctggagaga ttgatgatga agacaaagac
     3121 aaggatgaaa ctgaaacagt taagcagact caaacatctg aggtgtatga tggtcccaaa
     3181 aatgtaagat ctgatatttc tgatcaagag gaagatgaag aaagtgaagg atgtccagtg
     3241 tctattaatt tgtctaaagc tgaaactcag gctttaacta attatggaag tggagaagat
     3301 gaaaatgagg atgaagaaat ggaagaattt gaagaaggcc ctgtggatgt ccagacttcc
     3361 ctccaggcta acactgaagc tactgaagaa aatgaacatg atgaacaggt cctacaacgt
     3421 gactttaaaa agacagcaga aagcaaaaat gtcccattgg aacgagaagc cactagtaaa
     3481 aatgaccaaa ataactgtcc tgtgaaaccc tgttacctca atatcttgga agatgagcaa
     3541 cctttaaata gtgctgccca taaggagtca cctcctactg ttgattcaac tcaacagcct
     3601 aaccctttgc cgttacgttt acctgaaatg gaacccttag tgcctagagt caaagaagtt
     3661 aaatctgctc aggaaactcc tgaaagctct ctggctggaa gtcctgatac tgaatctcca
     3721 gtgttagtga atgactatga agcagaatct ggtaatataa gtcaaaagtc tgatgaagaa
     3781 gattttgtaa aagttgaaga tttaccactg aaactgacaa tatattcaga ggcagatcta
     3841 agaaagaaaa tggtagaaga agaacagaaa aaccatttat ctggtgaaat atgtgaaatg
     3901 cagaccgaag aattagctgg aaattctgag acactaaaag aacctgaaac ggtgggagcc
     3961 cagagtatat gtacagacta caaagaccat gacggtgatt ataaagatca tgacatcgac
     4021 tacaaggatg acgatgacaa gtaactcgag ataatcaacc tctggattac aaaatttgtg
     4081 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     4141 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     4201 aatcctggtt agttcttgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     4261 caggggctcg gctgttgggc actgacaatt ccgtggtgtt tatttgtgaa atttgtgatg
     4321 ctattgcttt atttgtaacc atctagcttt atttgtgaaa tttgtgatgc tattgcttta
     4381 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     4441 tttcaggttc agggggagat gtgggaggtt ttttaaagat ctggagaccg gtgtggcacg
     4501 tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg
     4561 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
     4621 cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc
     4681 tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc
     4741 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4801 tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4861 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4921 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     4981 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     5041 gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt tataagggat
     5101 tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     5161 ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga
     5221 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     5281 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     5341 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     5401 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     5461 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5521 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     5581 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5641 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5701 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5761 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     5821 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5881 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5941 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     6001 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     6061 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     6121 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     6181 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     6241 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     6301 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     6361 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6421 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     6481 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6541 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6601 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6661 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6721 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     6781 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6841 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6901 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6961 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     7021 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     7081 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     7141 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     7201 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//