| 质粒名称: |
pAAVLINKv1-pCALM1-Cre-3'-BRWD3-FLAG
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001666 |
| 产品用途: | 3' AAVLINK plasmid for BRWD3 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | BRWD3 |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-BRWD3-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-BRWD3-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-BRWD3-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-BRWD3-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-BRWD3-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-BRWD3-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-BRWD3-FLAG 6718 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6718)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6718
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3431
/codon_start=1
/label=BRWD3
/translation="GINHLLSLDFASPFAVPVDLSAYPLYCTVVAYPTDLNTIRRRLEN
RFYRRISALMWEVRYIEHNARTFNEPDSPIVKAAKIVTDVLLRFIGDQSCTDILDTYNK
IKAEERNSTDAEEDTEIVDLDSDGPGTSSGRRVKCRGRRQSLKCNPDAWKKQCKELLSL
IYEREDSEPFRQPADLLSYPGHQEQEGESSESVVPERQQDSSLSEDYQDVIDTPVDFST
VKETLEAGNYGSPLEFYKDVRQIFNNSKAYTSNKKSRIYSMMLRLSALFESHIKNIISE
YKSAIQSQKRRRPRYRKRLRSSSSSLSSSGAPSPKGKQKQMKLQPKNDQNTSVSHARTS
SPFSSPVSDAAEGLSLYLLDDEPDGPFSSSSFGGYSRSGNSHDPGKAKSFRNRVLPVKQ
DHSLDGPLTNGDGREPRTGIKRKLLSASEEDENMGGEDKEKKETKEKSHLSTSESGELG
SSLSSESTCGSDSDSESTSRTDQDYVDGDHDYSKFIQTRPKRKLRKQHGNGKRNWKTRG
TGGRGRWGRWGRWSRGGRGRGGRGRGSRGRGGGGTRGRGRGRGGRGASRGATRAKRARI
ADDEFDTMFSGRFSRLPRIKTRNQGRRTVLYNDDSDNDNFVSTEDPLNLGTSRSGRVRK
MTEKARVSHLMGWNY"
misc_feature 1828..1833
/label=SDS
misc_feature 3035..3040
/label=SDS
misc_feature 3077..3082
/label=SDS
misc_feature 3092..3097
/label=SDS
misc_feature 3158..3163
/label=SDS
misc_feature 3285..3290
/label=SDS
CDS 3438..3503
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3507..3938
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3651..3656
/label=SDS
repeat_region 3981..4121
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3981..4110
/label=AAV2 ITR
rep_origin 4196..4651
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4933..5037
/gene="bla"
/label=AmpR promoter
CDS 5038..5898
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6069..6657
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggcatcaac caccttcttt
1501 ccctggattt tgccagccct tttgctgttc cagtggatct cagtgcctac cccttgtatt
1561 gtactgtagt tgcttatcca actgacctca ataccatcag gcggagactt gaaaatcgct
1621 tttacaggag aatatcagca ttaatgtggg aggtacgcta tattgaacat aatgccagga
1681 ctttcaatga gccagacagt cctatagtta aagcagctaa aattgtaact gatgtcttac
1741 ttcgatttat tggggatcag agctgtactg atatactgga tacttataat aaaattaaag
1801 cagaagaacg aaacagtact gatgcagagg aggatacaga aattgttgat ttagattcag
1861 acggtcctgg tacttcatct ggaagaaggg tcaaatgccg aggcagaaga cagtctttaa
1921 agtgtaatcc tgatgcttgg aaaaaacaat gcaaggaact attgagcctc atttatgaac
1981 gtgaagactc ggagccattt cgacagccag ctgatcttct ttcttaccca ggtcatcagg
2041 agcaagaggg agaatcctca gagtctgttg ttccagaaag acaacaagat tcatctcttt
2101 ctgaggatta tcaagatgtt atagatactc ctgtggactt cagcactgtg aaagaaactt
2161 tggaagcagg aaactatggt agtcctctgg aattttataa ggatgttcgc caaatattca
2221 acaactccaa agcttatacc tctaataaaa agtcaaggat ctatagcatg atgctgcgat
2281 tatctgcctt atttgaaagt catatcaaaa atatcatctc tgaatataag tcagcaatcc
2341 agagtcagaa gaggagaagg ccacggtaca gaaaacgtct aagaagcagc agcagttcat
2401 tatctagtag tggagcacct agtccaaaag ggaagcagaa acaaatgaag ttgcagccta
2461 aaaatgacca gaatacctca gtatctcatg ccaggactag ctctcctttc tcatctcctg
2521 tttcagatgc tgctgaaggg ctttcactat atctacttga tgatgagcca gatgggccat
2581 tttcttcatc gagcttcggt ggatatagcc gaagtggaaa tagccatgac ccagggaaag
2641 ccaaatcatt tcggaataga gttttgccag ttaaacaaga tcactccctt gatggacccc
2701 ttacaaatgg tgatggcaga gagccccgga caggaatcaa gagaaaacta cttagtgcat
2761 cagaagaaga tgaaaacatg ggaggagaag ataaagagaa aaaagaaaca aaagagaaat
2821 ctcatttatc cacctcagag agtggagagt taggatccag tttaagttct gaaagtacct
2881 gtggttctga ctctgactct gaatcaactt ccagaacaga tcaagattac gtagatggag
2941 accatgatta tagcaaattc atacaaacca gacctaaaag aaaactcaga aagcaacatg
3001 gcaatggaaa aagaaattgg aagaccagag gcacaggagg aagaggcaga tggggaagat
3061 ggggtagatg gagtagagga ggcagaggaa gaggaggcag gggacgaggg agtcgaggaa
3121 gaggtggagg tggcactagg gggaggggaa gagggagagg aggaagaggt gcttctagag
3181 gagctaccag agccaaacga gcacgtattg cagatgatga atttgatacc atgttttcag
3241 gacgtttcag tagactgcct cgaattaaaa caagaaacca aggcaggagg actgttttat
3301 ataatgatga ttctgataat gacaattttg tgtccactga ggatcctctg aatcttggta
3361 catccagatc aggcagagtg cgtaaaatga cagaaaaagc cagggttagc catctcatgg
3421 gatggaatta ttgtacagac tacaaagacc atgacggtga ttataaagat catgacatcg
3481 actacaagga tgacgatgac aagtaactcg agataatcaa cctctggatt acaaaatttg
3541 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
3601 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
3661 taaatcctgg ttagttcttg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
3721 gacaggggct cggctgttgg gcactgacaa ttccgtggtg tttatttgtg aaatttgtga
3781 tgctattgct ttatttgtaa ccatctagct ttatttgtga aatttgtgat gctattgctt
3841 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
3901 tgtttcaggt tcagggggag atgtgggagg ttttttaaag atctggagac cggtgtggca
3961 cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
4021 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
4081 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
4141 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
4201 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
4261 cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
4321 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
4381 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
4441 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
4501 ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga tttataaggg
4561 attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
4621 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
4681 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
4741 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
4801 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
4861 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
4921 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
4981 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
5041 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
5101 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
5161 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
5221 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
5281 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
5341 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
5401 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
5461 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
5521 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
5581 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
5641 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
5701 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
5761 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
5821 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
5881 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
5941 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
6001 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
6061 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
6121 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
6181 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
6241 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
6301 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
6361 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
6421 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
6481 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
6541 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
6601 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
6661 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
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