pAAVLINK-EF1a-5'-BRWD3

LOCUS       pAAVLINK-EF1a-5'-BRWD3            7064 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 1, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        158..700
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    505..510
                     /label=SDS
     CDS             716..742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             749..4204
                     /codon_start=1
                     /label=BRWD3
                     /translation="MAAAPTQIEAELYYLIARFLQSGPCNKSAQVLVQELEEHQLIPRR
                     LDWEGKEHRRSFEDLVAANAHIPPDYLLKICERIGPLLDKEIPQSVPGVQTLLGVGRQS
                     LLRDAKDCKSTLWNGSAFAALHRGRPPELPVNYVKPPNVVNITSARQLTGCSRFGHIFP
                     SSAYQHIKMHKRILGHLSSVYCVAFDRSGRRIFTGSDDCLVKIWATDDGRLLATLRGHS
                     AEISDMAVNYENTLIAAGSCDKVVRVWCLRTCAPVAVLQGHSASITSIQFCPSTKGTNR
                     YLTSTGADGTICFWQWHVKTMKFRDRPVKFTERSRPGVQISCSSFSSGGMFITTGSTDH
                     VIRIYYLGSEVPEKIAELESHTDKVVAVQFCNNGDSLRFVSGSRDGTARIWQYQQQEWK
                     SIVLDMATKMTGNNLPSGEDKITKLKVTMVAWDRYDTTVITAVNNFLLKVWNSITGQLL
                     HTLSGHDDEVFVLEAHPFDQRIILSAGHDGNIFIWDLDRGTKIRNYFNMIEGQGHGAVF
                     DCKFSPDGNHFACTDSHGHLLLFGFGCSKYYEKIPDQMFFHTDYRPLIRDANNYVLDEQ
                     TQQAPHLMPPPFLVDVDGNPHPTKFQRLVPGRENCKDEQLIPQLGYVANGDGEVVEQVI
                     GQQTNDQDESILDGIIRELQREQDLRLINEGDVPHLPVNRAYSVNGALRSPNMDISSSP
                     NIRLRRHSSQIEGVRQMHNNAPRSQMATERDLMAWSRRVVVNELNNGVSRVQEECRTAK
                     GDIEISLYTVEKKKKPSYTTQRNDYEPSCGRSLRRTQRKRQHTYQTRSNIEHNSQASCQ
                     NSGVQEDSDSSSEEDETVGTSDASVEDPVVEWQSESSSSDSSSEYSDWTADAGINLQPP
                     KRQTRQTTRKICSSSDEENLKSLEERQKKPKQTRKKKGGLVSIAGEPNEEWFAPQWILD
                     TIPRRSPFVPQMGDELIYFRQGHEAYVRAVRKSKIYSVNLQKQPWNKMDLREQEFVKIV
                     GIKYEVGPPTLCCLKLAFLDPISGKMTGESFSIKYHDMPDVIDFLVLHQFYNEAKERNW
                     QIGDRFRSIIDDAWWFGTVESQQPFQPEYPDSSFQCYSVHWDNNEREKMSPWDMEPIPE
                     GTAFPDEVGAGVPVSQEELTALLYKPQEGEWGAHSRDEECERVIQ"
     RBS             960..968
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    961..966
                     /label=SDS
     misc_feature    2501..2506
                     /label=SDS
     misc_feature    3472..3477
                     /label=SDS
     intron          4205..4245
                     /label=Splicing Donor
     misc_feature    4249..4282
                     /label=lox2272-JT1571
     repeat_region   4327..4467
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4327..4456
                     /label=AAV2 ITR
     rep_origin      4542..4997
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5279..5383
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5384..6244
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6415..7003
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
      721 ttatgacgta cctgactatg ctggatctat ggcggcagca cctacccaga tcgaagccga
      781 gctgtattac ctgatcgcta ggttcttgca gtctggaccc tgcaacaaat ccgctcaggt
      841 gctagtgcag gagctcgagg agcatcagct gattccgcgc cgcttagatt gggaggggaa
      901 agagcaccga agaagcttcg aggatctggt ggcagcaaat gcacacattc ctccagacta
      961 cctccttaaa atttgtgaga gaattggtcc tttactagat aaagagatcc ctcagagtgt
     1021 tcctggggta cagacattat taggtgttgg tcggcagtct ctgctacggg atgccaaaga
     1081 ctgtaagagt acactatgga atgggtctgc ttttgcggct ctgcatagag gcagacctcc
     1141 agaactacct gtaaattatg tgaaacctcc aaatgtggtg aatatcacct ctgccaggca
     1201 attaaccggc tgtagtcgct ttggtcatat tttcccttca tctgcttacc agcacattaa
     1261 gatgcataag agaattctgg ggcacttgtc atctgtctac tgtgtagcat ttgaccgaag
     1321 cgggagaaga atttttacag gttcagatga ctgtttagta aaaatttggg ctacagatga
     1381 tggacgcctt cttgctacac ttcgtggaca ctctgctgaa atttctgaca tggctgttaa
     1441 ctatgaaaac actcttattg ctgcaggcag ctgtgataag gtagtaagag tatggtgtct
     1501 tcgaacttgt gcacccgttg cagtccttca gggccattca gcttctatta cttccataca
     1561 gttttgtcca tcaactaaag gcacaaacag atacctcact tctactggtg ctgatggaac
     1621 aatctgtttc tggcaatggc atgtaaaaac aatgaagttt agagatcgcc cggtgaaatt
     1681 tactgagaga tccagacctg gagtccagat atcttgttca tctttcagtt ctggtggtat
     1741 gttcattaca actggtagta ctgaccatgt gattagaata tattatttgg gttctgaggt
     1801 tcctgagaaa attgctgaat tagagtcaca tacggataaa gttgttgctg ttcagttctg
     1861 taacaatgga gacagtttaa gatttgttag tggaagtcga gatggaacgg caagaatttg
     1921 gcagtatcag caacaagaat ggaagagtat agtgctagac atggctacta aaatgactgg
     1981 caataatttg ccatctggag aagacaagat cactaaactt aaggtgacta tggtggcctg
     2041 ggatcgctat gataccacag ttattactgc agtgaacaat tttcttttga aagtgtggaa
     2101 ttctatcaca ggacagcttc ttcatacatt atctggacat gatgatgaag tattcgttct
     2161 agaagcccat ccatttgatc aaaggatcat actttcagca ggtcatgatg ggaacatttt
     2221 tatttgggac cttgaccggg ggaccaaaat tcggaattac tttaacatga ttgaaggcca
     2281 aggccatggt gcggtgtttg attgtaaatt ttcaccagat ggaaaccatt ttgcctgcac
     2341 agattctcat ggacatttgc tgctttttgg ttttggatgc agtaaatact acgaaaagat
     2401 tccagatcag atgttcttcc acacggatta tcgtcctctt attcgtgatg ccaataacta
     2461 tgtattggat gaacaaaccc aacaagctcc tcacctcatg cctcctccat ttttggtgga
     2521 tgttgatgga aatcctcatc ccacaaaatt ccaacggttg gtaccaggac gggaaaattg
     2581 taaagatgaa cagcttatac cacagctggg atatgtggct aatggtgatg gtgaggtagt
     2641 agaacaggta attgggcagc aaaccaatga ccaagatgag agcattcttg atggaataat
     2701 cagggagctg cagagagaac aagacctgag actaattaat gaaggagatg ttccacattt
     2761 accagttaat agagcatact ctgttaatgg tgctctgaga agtccaaaca tggacatatc
     2821 ttcttcccca aacatcaggc ttcgaagaca tagtagtcaa attgaaggtg ttagacaaat
     2881 gcataacaat gctcctcgga gccagatggc cactgaaaga gatctcatgg cgtggagcag
     2941 aagagtggtg gtcaatgaac taaataatgg ggttagtagg gtacaggaag aatgtcgaac
     3001 tgcaaaaggt gacatagaaa tcagtcttta tacagttgaa aagaagaaaa agccatctta
     3061 cactactcaa aggaatgatt atgagcctag ctgtgggcgt tctttacgca ggacacagcg
     3121 caaacgtcag catacttacc aaacacggtc caacatagag cataattcac aggcatcatg
     3181 tcaaaattca ggtgtacagg aagactcaga cagttcttca gaggaagatg aaactgttgg
     3241 cacaagtgac gcttcggtag aggatcctgt tgttgaatgg caaagtgaaa gttcttccag
     3301 tgactcatca agtgaatatt ctgattggac agcagatgct ggaataaatt tacaaccccc
     3361 aaaaagacaa accagacaga caacacgtaa aatatgcagc agctctgatg aggaaaattt
     3421 gaagtcccta gaagaaaggc agaagaaacc taagcagact agaaagaaga aaggaggact
     3481 ggtttctata gcaggtgagc ctaatgaaga atggtttgcc cctcagtgga tcttggatac
     3541 tataccccga cgttccccat ttgtcccaca aatgggagat gagcttatct attttaggca
     3601 aggacatgaa gcttatgttc gggctgtaag gaaatcgaaa atatacagtg ttaatttaca
     3661 aaaacagcca tggaacaaaa tggatctcag ggagcaagag tttgttaaga ttgtaggaat
     3721 caaatatgag gttggcccac ccacactgtg ctgcttgaag cttgcatttc tggacccaat
     3781 ttcaggcaaa atgactggag aatctttttc cattaagtat catgacatgc cggatgtcat
     3841 tgacttcctt gtgctacatc agttttataa tgaagccaaa gaaaggaact ggcagattgg
     3901 tgatagattc cgcagtataa tagatgacgc ctggtggttt gggactgtgg agagtcagca
     3961 gccttttcaa ccagagtatc ctgatagttc tttccagtgt tacagtgttc actgggacaa
     4021 taatgagaga gaaaagatga gcccatggga tatggagcca attccagaag gaactgcctt
     4081 tccagatgaa gttggtgctg gtgttcctgt ctcccaggaa gaattgactg ctttgctata
     4141 caaaccccag gaaggagagt ggggggctca ttccagagac gaagaatgtg aacgggttat
     4201 tcaggtaagt atcaaggtta caagacaggt ttaaggagac caataagcta ttattcgtat
     4261 aggatacttt atacgaagtt attaagttat ggagaccggt gtggcacgtg cggaccgagc
     4321 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
     4381 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
     4441 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
     4501 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     4561 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt
     4621 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4681 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4741 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4801 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4861 aacaacactc aactctatct cgggctattc ttttgattta taagggattt tgccgatttc
     4921 ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     4981 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     5041 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     5101 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     5161 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     5221 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     5281 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5341 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5401 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5461 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5521 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     5581 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     5641 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5701 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5761 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     5821 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     5881 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     5941 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     6001 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     6061 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     6121 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     6181 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     6241 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6301 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6361 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6421 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6481 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6541 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     6601 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6661 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6721 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6781 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6841 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6901 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6961 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     7021 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//