| 质粒名称: | pAAVLINK-EFS-HA-5'-PRR14L |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001431 |
| 产品用途: | 5' AAVLINK plasmid for PRR14L expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EFS |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: |
PRR14L
|
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EFS-HA-5'-PRR14L载体质粒基本信息
pAAVLINK-EFS-HA-5'-PRR14L质粒图谱载体图谱和pAAVLINK-EFS-HA-5'-PRR14L载体序列质粒序列多克隆位点信息
pAAVLINK-EFS-HA-5'-PRR14L质粒载体简介
pAAVLINK-EFS-HA-5'-PRR14L质粒序列载体序列
LOCUS pAAVLINK-EFS-HA-5'-PRR14L 7310 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7310)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 7, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..7310
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..4011
/codon_start=1
/label=PRR12
/translation="GAPGWSP*SHLRSSCLCPRLARVWEPHRVMPYQALTTTAWTRA*L
GRRSRPRLRRWRRSSRR*SRVSWPPSWTSSSQASATHHSTRRA*RLRSALPRVCHPLCQ
PEACSPSPLPPLLCHIPHLPEPLGLGAPWRLQRVRVWGLAALHPASGLMRS*SGTSRRC
PPSPRMRKTLSPRTETCRRASPPPSLPSMTHPLLGQKTLPPQMGRPWPPRLQFQGHPLF
RGSPVPTAMALPSPRCWRRNPHPLHLLPRLLSLSLRHPLRRHSQPCPRHPRWWPPRPAH
HRHRRCRRHLHQPCPRLHHHPHQPLPHWLLLLRSPPPRLPKTPSCRTPGPCIWPKSRRR
RQCVGRRTRRPARVAERASSGNGTSSSSVLRTSLPSSWRCRRGVNPHPSGESRRPFCRN
SLRRSRTARGSFVPPVIIWGILGMQKIGTSASM*SSWKMSIRRTT*GSVLGNPGIGPQC
QSDALGRPRTPYLLGVALHLPLRPQHHLPSLRPLKRRHLRSPQSRLLRRPCLSPLPPRS
PPSCGLLRRKRRRRR*HVESGHCGVSGPPADGRHGQSGVSPRDNLPHPGCPKPGLPR*R
LNRPLRRGRNG*RRQAATLQQAGAHQAAPRTRSPPLEPPARTSGQYLGVCSKPGRCGRC
TGATWRCW*AQHLTQT*SRPWRTRMTSCTCPPCGR*TAC*MSTRRKS*SGCR*AQPCRM
LCTRSHSCKWSRVGRALRKRGLCGCGLLGNPTTARRSASSRGAWSEPRSSRLSWKSRDT
IHSTIRSTTINTTPSCAAGTRPWPSRAAPRTWARRRWSSSACGTSRGWNSSLTPSVTCW
PKHRPTAAA"
RBS 1704..1712
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
misc_feature 1706..1711
/label=SDS
misc_feature 1751..1756
/label=SDS
misc_feature 2036..2041
/label=SDS
CDS complement(2110..2121)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
misc_feature 2287..2292
/label=SDS
misc_feature 2479..2484
/label=SDS
misc_feature 2591..2596
/label=SDS
misc_feature 3102..3107
/label=SDS
misc_feature 3311..3316
/label=SDS
misc_feature 3905..3910
/label=SDS
misc_feature 3908..3913
/label=SDS
CDS 4018..4083
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4099..4530
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4243..4248
/label=SDS
repeat_region 4573..4713
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4573..4702
/label=AAV2 ITR
rep_origin 4788..5243
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5525..5629
/gene="bla"
/label=AmpR promoter
CDS 5630..6490
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6661..7249
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggggg cacccggttg
1561 gagcccctga agccacttaa gatcaagctg tctgtgccca aggctggcga gggtctggga
1621 acctcatcgg gtgatgccat atcaggcact gaccacaaca gcctggactc gagcctgact
1681 cgggagaaga tcgaggccaa gattaaggag gtggaggaga agcagccgga gatgaagtcg
1741 ggtttcatgg cctccttctt ggacttcctc aagtcaggca agcgccaccc accactctac
1801 caggcgggcc tgacgcctcc gctcagccct cccaagagtg tgccaccctc tgtgccagcc
1861 cgaggcctgc agccccagcc ccctgccacc cctgctgtgc cacatccccc accttccgga
1921 gcctttgggc ttgggggcgc cctggaggct gcagagagtg agggtctggg gcttggctgc
1981 ccttcaccct gcaagcggct tgatgaggag ctgaagcgga acctcgagac gctgccctcc
2041 ttctcctcgg atgaggaaga ctctgtcgcc aagaaccgag acctgcagga gagcatctcc
2101 tccgccatct ctgccctcga tgacccaccc cttgctgggc caaaagacac ttccacccca
2161 gatgggccgc ccttggcccc cgcggctgca gttccagggc caccccctct tccggggctc
2221 cccagtgcca acagcaatgg cactcccgag cccccgctgc tggaggagaa acccccaccc
2281 actccacctc ctgccccgac tcctcagcct cagcctccgc caccccctcc gccgccacag
2341 ccagccctgc cctcgccacc cccgctggtg gcccccacgc ccagctcacc accgccaccg
2401 ccgctgccgc cgccacctcc accagccatg ccctcgcctc caccaccacc cccaccagcc
2461 gctgccccac tggctgctcc tcctgaggag cccgccgccc cgtctcccga agaccccgag
2521 ctgccggaca cccggcccct gcatctggcc aaaaagcagg agacggcggc agtgtgtggg
2581 gagacggacg aggaggccgg cgagagtggc ggagagggca tcttccggga acgggacgag
2641 ttcgtcatcc gtgctgagga catcccttcc ctcaagctgg cgttgcagac ggggcgtgaa
2701 cccccaccca tctggcgagt ccagaaggcc cttctgcaga aattcactcc ggagatcaag
2761 gacggccaga ggcagttttg tgccaccagt aattatttgg ggtattttgg ggatgcaaaa
2821 aatcggtacc agcgcctcta tgtaaagttc ctggaaaatg tcaataagaa ggactacgtg
2881 agggtctgtg ctcggaaacc ctggcatcgg cccccagtgc cagtcagacg ctctgggcag
2941 gccaagaacc ccgtatctgc tgggggtagc tctgcacctc cccctaaggc cccagcacca
3001 cctcccaagc ctgagacccc tgaaaagacg acatctgaga agcccccaga gcagactcct
3061 gagacggcca tgcctgagcc ccctgccccc gagaagccct ccctcctgcg gcctgttgag
3121 aaggaaaagg agaaggagaa ggtgacacgt ggagagcggc cattgcgggg tgagcgggcc
3181 accagcggac ggcagacacg gccagagcgg agtctcgcca cgggacaacc tgccacatcc
3241 cggctgccca aagcccggcc taccaaggtg aaggctgaac cgccccctaa gaagaggaag
3301 aaatggctga aggaggcagg cggcaacgct acagcaggcg ggggcccacc aggcagctcc
3361 tcggactcgg agtcctcccc tggagccccc agcgaggacg agcgggcagt acctgggcgt
3421 ctgctcaaaa ccagggcgat gcgggagatg taccggagct acgtggagat gttggtgagc
3481 acagcacttg acccagacat gatccaggcc ctggaggaca cgcatgacga gctgtacctg
3541 ccccccatgc ggaagataga cggcctgctg aatgagcaca agaagaaagt cctgaagcgg
3601 ctgtcgctaa gcccagccct gcaggatgct ctgcacacgt tcccacagct gcaagtggag
3661 cagagtgggg agggctctcc ggaagagggg gctgtgcggc tgcggcctgc tggggaaccc
3721 tacaaccgca agacgctcag caagctcaag aggagcgtgg tcagagccca ggagttcaag
3781 gttgagctgg aaaagtcggg atactataca ctctaccatt cgctccacca ctataaatac
3841 cacaccttcc tgcgctgccg ggaccagacc ctggccatcg agggcggcgc cgaggacctg
3901 ggccaggagg aggtggtcca gcagtgcatg cggaaccagc cgtggctgga acagctcttt
3961 gactccttca gtgacctgct ggcccaagca caggcccaca gccgctgcgg gtgtacagac
4021 tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac
4081 aagtaagaat tctgatgact cgagataatc aacctctgga ttacaaaatt tgtgaaagat
4141 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
4201 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
4261 ggttagttct tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
4321 ctcggctgtt gggcactgac aattccgtgg tgtttatttg tgaaatttgt gatgctattg
4381 ctttatttgt aaccatctag ctttatttgt gaaatttgtg atgctattgc tttatttgta
4441 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
4501 gttcaggggg agatgtggga ggttttttaa agatctggag accggtgtgg cacgtgcgga
4561 ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
4621 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
4681 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
4741 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
4801 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
4861 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
4921 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
4981 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
5041 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
5101 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc
5161 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
5221 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
5281 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
5341 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
5401 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
5461 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
5521 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
5581 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
5641 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
5701 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
5761 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
5821 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
5881 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
5941 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
6001 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
6061 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
6121 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
6181 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
6241 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
6301 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
6361 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
6421 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
6481 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
6541 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
6601 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
6661 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
6721 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
6781 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
6841 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
6901 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
6961 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
7021 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
7081 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
7141 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
7201 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
7261 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
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pAAVLINK-EFS-HA-5'-PRR14L质粒载体应用举例
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