| 质粒名称: | pMTMVi-N |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 118756 |
| 存储实验室: | Jose-Antonio Daros |
|
载体骨架基本信息 |
|
| 载体名称: | pUC18 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 2376 bp |
| 载体修饰: | -- |
| 载体类型: | 构建烟草花叶病毒重组克隆的中间产物 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Tobacco mosaic virus cDNA fragment with a cloning polylinker |
| 物种来源: | Tobacco mosaic virus |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMTMVi-N was a gift from Jose-Antonio Daros (Addgene plasmid # 118756 ; http://n2t.net/addgene:118756 ; RRID:Addgene_118756) |
| 参考文献部分: | Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus. Shi X, Cordero T, Garrigues S, Marcos JF, Daròs JA, Coca M.. Plant Biotech Journal (2018) 10.1111/pbi.13038 |
买家导航
pMTMViN载体基本信息
pMTMViN载体图谱质粒图谱和多克隆位点信息
pMTMViN载体简介
pMTMViN载体序列
LOCUS pMTMVi-N 2376 bp ds-DNA circular SYN 20-FEB-2019
DEFINITION Intermediate plasmid to build tobacco mosaic virus recombinant
clones that do not move systemically throughout the plant.
ACCESSION .
VERSION .
KEYWORDS pMTMVi-N
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2376)
AUTHORS Shi X, Cordero T, Garrigues S, Marcos JF, Daros JA, Coca M.
TITLE Efficient production of antifungal proteins in plants using a new
transient expression vector derived from tobacco mosaic virus.
JOURNAL Plant Biotech Journal (2018)
REFERENCE 2 (bases 1 to 2376)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Feb 20, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..2376
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 358..379
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 394..424
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 432..448
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 437..459
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 456..472
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 456..472
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
CDS 468..773
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITPSAQLTLTKGNKSWVPNSPYSESYYARSLAVVLQRRDWEN
PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDALEVLQLEVVKMHNK"
primer_bind 491..511
/label=T3
/note="T3 promoter, forward primer"
promoter 493..511
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(537..556)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(538..556)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(566..583)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(566..582)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(575..597)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin complement(857..1445)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(937..956)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1457..2317)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 2080..2099
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
ORIGIN
1 agattacaaa cgtgagagac ggagggccca tggaacttac agaagaagtc gttgatgagt
61 tcatggaaga tgtccctatg tcgatcaggc ttgcaaagtt tcgatctcga accggaaaaa
121 agagtgatgt ccgcaaaggg aaaaatagta gtagtgatcg gtcagtgccg aacaagaact
181 atagaaatgt taaggatttt ggaggaatga gttttaaaaa gaataattta atcgatgatg
241 attcggaggc tactgtcgcc gaatcggatt cgttttaaat agatcttaca gtatcactac
301 tccatctcag ttcgtgttct tgtcaccggt gaaagcgggc agtgagcgca acgcaattaa
361 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgctcc cggctcgtat
421 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
481 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctgggtaccc aattcgccct
541 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
601 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
661 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
721 gggacgcgct cgaggtcctg caacttgagg tagtcaagat gcataataaa taacggattg
781 tgtccgtaat cacacgtggt gcgtacgata acgcatagtg tttttccctc cacttggaga
841 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
901 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
961 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
1021 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
1081 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
1141 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
1201 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
1261 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
1321 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
1381 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
1441 ctcaagaaga tcctttttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
1501 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
1561 gcttaccatc tggccccagt gctgcaatga taccgcgaga gccacgctca ccggctccag
1621 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
1681 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
1741 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
1801 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
1861 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
1921 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
1981 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
2041 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
2101 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
2161 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
2221 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
2281 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
2341 gaagcattta tcagggttat tgtctcatgg tctcga
//
