| 质粒名称: | pAAVLINK-EF1a-HA-5'-PCM1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001617 |
| 产品用途: | 5' AAVLINK plasmid for PCM1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PCM1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-PCM1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-PCM1载体质粒基本信息
pAAVLINK-EF1a-HA-5'-PCM1质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-PCM1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-PCM1质粒载体简介
pAAVLINK-EF1a-HA-5'-PCM1质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-PCM1 7257 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7257)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 20, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7257
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..4397
/codon_start=1
/label=PCM1
/translation="MATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQKKANRSS
EKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRYMSQMSVPEQAELEKLK
QRINFSDLDQRSIGSDSQGRATAANNKRQLSENRKPFNFLPMQINTNKSKDASTSPPNR
ETIGSAQCKELFASALSNDLLQNCQVSEEDGRGEPAMESSQIVSRLVQIRDYITKASSM
REDLVEKNERSANVERLTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHD
LLKRMLQQQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSELNE
ELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASERLPDEKVELFSK
MRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQRSTSAPSASVGLAPVVNGES
NSLTSSVPYPTASLVSQNESENEGHLNPSEKLQKLNEVRKRLNELRELVHYYEQTSDMM
TDAVNENRKDEETEESEYDSEHENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGR
TVNSNCEINNRSAANIRALNVPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSG
ASLSSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGVISASAS
NLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAKLQQQQRELKQLQEER
KKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKYMPAVTSTPTVNQHETSTSKSVFEP
EDSSIVDNELWSEMRRHEMLREELRQRRKQLEALMAEHQRRQGLAETASPVAVSLRSDG
SENLCTPQQSRTEKTMATWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDN
FSVCPSNSVNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVSE
LSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGPYSVMPSNVAS
PQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQHPEKPGGKERGSSASHPPS
PSLFCPFSFPTQPVNLFNIPGFTNFSSFAPGMNFSPLFPSNFGDFSQNISTPSEQQQPL
AQNSSGKTEYMAFPKPFESSSSIGAEKPRNKKLPEEEVESSRTPWLYEQE"
misc_feature 757..762
/label=SDS
misc_feature 760..765
/label=SDS
misc_feature 2540..2545
/label=SDS
misc_feature 2613..2618
/label=SDS
misc_feature 2616..2621
/label=SDS
misc_feature 2619..2624
/label=SDS
misc_feature 2637..2642
/label=SDS
misc_feature 3302..3307
/label=SDS
misc_feature 3501..3506
/label=SDS
misc_feature 4061..4066
/label=SDS
misc_feature 4353..4358
/label=SDS
intron 4398..4438
/label=Splicing Donor
misc_feature 4442..4475
/label=lox2272-JT1571
repeat_region 4520..4660
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4520..4649
/label=AAV2 ITR
rep_origin 4735..5190
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5472..5576
/gene="bla"
/label=AmpR promoter
CDS 5577..6437
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6608..7196
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat ggccacagga ggaggtccct ttgaagatgg
781 catgaatgat caggatttac caaactggag taatgagaat gttgatgaca ggctcaacaa
841 tatggattgg ggtgcccaac agaagaaagc aaatagatca tcagaaaaga ataagaaaaa
901 gtttggtgta gaaagtgata aaagagtaac caatgatatt tctccggagt cgtcaccagg
961 agttggaagg cgaagaacaa agactccaca tacgttccca cacagtagat acatgagtca
1021 gatgtctgtc ccagagcagg cagaattaga gaaactgaaa cagcggataa acttcagtga
1081 tttagatcag agaagcattg gaagtgattc ccaaggtaga gcaacagctg ctaacaacaa
1141 acgtcagctt agtgaaaacc gaaagccctt caactttttg cctatgcaga ttaatactaa
1201 caagagcaaa gatgcatcta caagtccccc aaacagagaa acgattggat cagcacagtg
1261 taaagagttg tttgcttctg ctttaagtaa tgacctcttg caaaactgtc aggtgtctga
1321 agaagatggg aggggagaac ctgcaatgga gagcagccag attgtaagca ggcttgttca
1381 aattcgcgat tatattacta aagctagttc catgcgggaa gatcttgtag agaaaaatga
1441 gagatctgct aatgttgagc gccttactca tctaatagat caccttaaag aacaagagaa
1501 gtcatatatg aaatttctta aaaaaatcct tgccagagat cctcagcagg agcctatgga
1561 agagatagaa aatttgaaga aacaacatga tttattaaaa agaatgttac aacagcagga
1621 gcaactaaga gctctacagg gacggcaggc tgcacttcta gctctgcaac ataaagcaga
1681 gcaagctatt gcagtgatgg atgattctgt tgttgcagaa actgcaggta gcttatctgg
1741 cgtcagtatc acatctgaac taaatgaaga attgaatgac ttaattcagc gttttcataa
1801 tcagcttcgt gattctcagc ctccagctgt tccagacaat agaagacagg cagaaagtct
1861 ttcattaact agggaggttt cccagagcag gaaaccatca gcttcagaac gtttacctga
1921 tgagaaagtc gaacttttta gcaaaatgag agtgctacag gaaaagaaac aaaaaatgga
1981 caaattgctt ggagaacttc atacacttcg agatcagcat cttaacaatt catcatcctc
2041 tccacaaagg agtgtcgatc agagaagtac ttcagctccc tctgcttctg taggcttggc
2101 accggttgtc aatggagaat ccaatagcct cacatcatct gttccttatc ctactgcttc
2161 tctagtatct cagaatgaga gtgaaaacga aggccacctc aatccatctg aaaaactcca
2221 gaagttaaat gaagttcgaa agagattgaa tgagctaaga gaattagttc attattatga
2281 acaaacgtca gacatgatga cagatgctgt gaatgaaaac aggaaagatg aagaaactga
2341 agagtcagaa tatgattctg agcatgaaaa ttccgagcct gttactaaca ttcgaaatcc
2401 acaagtagct tccacttgga atgaagtaaa tagtcatagt aatgcacagt gtgtttctaa
2461 taatagagat gggcgaacag ttaattctaa ttgtgaaatt aacaacagat ctgctgccaa
2521 cataagggct ctaaacgtgc ctccttcttt agattgtcga tataatagag aaggggaaca
2581 ggagattcat gttgcacaag gtgaagatga tgaggaggag gaggaagaag cagaagagga
2641 gggagtcagt ggagcttcat tatctagtca caggagcagt ctggttgatg agcatccaga
2701 agatgctgaa tttgaacaga agatcaaccg acttatggct gcaaaacaga aacttagaca
2761 gttacaagat cttgttgcta tggtacagga tgatgatgca gctcaaggag ttatctctgc
2821 cagtgcatca aatttggatg atttctaccc agcagaagaa gacaccaagc aaaattcaaa
2881 taacactaga ggaaatgcca ataaaacaca gaaagatact ggagtaaatg aaaaggcaag
2941 agagaaattt tatgaggcta aactacagca gcaacagaga gagctaaaac aattgcagga
3001 agaaagaaag aaactgattg acattcagga gaaaattcaa gcattgcaaa cggcatgccc
3061 tgacttacag ctgtcagctg ctagtgtggg taactgtcct accaaaaaat atatgccagc
3121 tgttacttca accccaactg ttaatcaaca cgagaccagt acaagcaaat ctgtttttga
3181 gcctgaagat tcttcaatag tagataatga gttgtggtca gaaatgagaa gacatgaaat
3241 gttgagggag gagctgcgac agagaagaaa gcagcttgaa gctctgatgg ctgaacatca
3301 gaggaggcaa ggtctagctg aaactgcatc tccagtggct gtgtcattga gaagtgatgg
3361 atctgagaac ctatgtactc ctcagcaaag tagaacagaa aaaacgatgg caacttgggg
3421 agggtctacc cagtgtgcac tagatgaaga aggagatgaa gacggttacc tttctgaagg
3481 aattgttcgg acagatgaag aggaggaaga agagcaagat gccagttcca atgataactt
3541 ttctgtgtgt ccttctaaca gtgtgaatca taactcctac aatggaaagg aaactaaaaa
3601 taggtggaag aacaattgcc ctttttcggc agatgaaaat tatcgtcctt tagccaagac
3661 aaggcaacag aatatcagca tgcaacggca agaaaacctt cgttgggtgt cagagctctc
3721 ttacgtagaa gagaaagaac aatggcaaga acaaatcaat cagctaaaga aacagcttga
3781 ttttagtgtc agtatttgtc agactttgat gcaagaccag cagactctat cttgtctgct
3841 acaaactctt ctcacgggtc cttacagtgt tatgcccagc aatgttgcat ctcctcaagt
3901 acacttcata atgcaccagt tgaaccagtg ctatactcag ctaacatggc aacagaataa
3961 tgttcagagg ttgaaacaaa tgctaaatga acttatgcgc cagcaaaatc agcatccaga
4021 aaaacctgga ggcaaggaaa gaggcagtag tgcatcgcac cctccttctc ccagtttatt
4081 ttgtcctttc agctttccaa cacagcctgt aaatctcttc aatatacctg gatttactaa
4141 cttttcatca tttgcaccag gtatgaattt cagcccttta tttccttcta attttggaga
4201 tttttctcag aatatctcta cacccagtga acagcagcaa cccttagccc agaattcttc
4261 aggaaaaaca gaatatatgg cttttccaaa accttttgaa agcagttcct ctattggagc
4321 agagaaacca aggaataaaa aactgcctga agaggaggtg gaaagcagta ggacaccatg
4381 gttatatgaa caagaaggta agtatcaagg ttacaagaca ggtttaagga gaccaataag
4441 ctattattcg tataggatac tttatacgaa gttattaagt tatggagacc ggtgtggcac
4501 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
4561 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
4621 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt
4681 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
4741 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
4801 ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
4861 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
4921 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
4981 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
5041 tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga
5101 ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
5161 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
5221 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
5281 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
5341 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
5401 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
5461 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
5521 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
5581 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
5641 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
5701 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
5761 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
5821 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
5881 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
5941 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
6001 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
6061 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
6121 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
6181 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
6241 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
6301 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
6361 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
6421 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
6481 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
6541 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
6601 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
6661 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
6721 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
6781 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
6841 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
6901 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
6961 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
7021 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
7081 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
7141 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
7201 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
pAAVLINK-EF1a-HA-5'-PCM1载体图谱质粒图谱pdf版和pAAVLINK-EF1a-HA-5'-PCM1载体序列质粒序列等相关资料下载
pAAVLINK-EF1a-HA-5'-PCM1质粒载体应用举例
- 上一篇：pAAVLINKv2-SCP1-Cre-UDeg3a-3'-POLR3A-FLAG
- 下一篇：没有了
