pAAVLINK-EF1a-HA-5'-MED23

LOCUS       pAAVLINK-EF1a-HA-5'-MED23               5802 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        146..688
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        175..386
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             399..667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    493..498
                     /label=SDS
     CDS             704..730
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             737..2942
                     /codon_start=1
                     /label=MED23
                     /translation="ETQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQ
                     FWGGLSQESHEQCIQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSD
                     TLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAR
                     EVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICG
                     RCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDM
                     VCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFF
                     VLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFD
                     LLYPEKEYIPVPDINKPQSTHAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQ
                     QSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASG
                     SITPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIE
                     SLGIKGFISQLLPTVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQT
                     NQNQLHLCVESTALRLITALGSSEVQPQFTRFLSDPKTVLSAESEELNRALILTLARAT
                     HVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFP"
     misc_feature    2311..2316
                     /label=SDS
     intron          2943..2983
                     /label=Splicing Donor
     misc_feature    2987..3020
                     /label=lox2272-JT1571
     repeat_region   3065..3205
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3065..3194
                     /label=AAV2 ITR
     rep_origin      3280..3735
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4017..4121
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4122..4982
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5153..5741
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
      181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
      241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
      421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
      481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
      541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
      601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
      661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
      721 tgactatgct ggatctgaga cgcaactgca gagcattttc gaagaggtgg tgaaaacgga
      781 agttatagaa gaggcttttc ctggcatgtt tatggatact cctgaagatg agaaaacaaa
      841 actaattagc tgtttggggg ccttcagaca gttttggggt ggtctttctc aggagtctca
      901 tgaacagtgt atccagtgga ttgttaagtt tattcatggt cagcatagtc ctaaaagaat
      961 ttcttttctt tatgactgct tagcaatggc agttgagact ggtctccttc cacccaggct
     1021 ggtttgtgaa tccctgataa actctgacac tcttgagtgg gaaagaacac agctttgggc
     1081 cttaacattt aaactggttc ggaaaataat tgggggagtg gattacaagg gtgttcgaga
     1141 tctcttaaaa gtgattttgg agaagatttt gacaattcct aatacagtga gctctgctgt
     1201 tgtacagcag cttctggcag caagagaggt tatagcatat atcttggaaa gaaatgcctg
     1261 cttattacca gcctattttg cagtcactga gatcaggaaa ctgtatcctg aaggcaaact
     1321 tccacactgg ttacttggaa acctagtatc agactttgtg gataccttca ggcccacagc
     1381 aaggataaac tccatttgtg gtcgctgtag tcttctgcca gttgtaaata attcgggtgc
     1441 catttgtaat tcatggaaac tggatcctgc tactcttcgt tttcctttga aaggcctttt
     1501 gccatatgat aaggatctgt ttgaaccaca gactgctttg ttgagatatg tattggagca
     1561 gccttattcc agggatatgg tctgcaatat gctaggttta aataagcagc acaagcagcg
     1621 ctgccctgtg ctggaggacc agttggtgga tctggttgtt tatgccatgg agcgatctga
     1681 gaccgaggag aagtttgacg atgggggaac aagccaactc ctgtggcagc atctctcaag
     1741 tcagctcatt ttctttgtgc ttttccagtt tgcaagtttt ccacatatgg tgctttctct
     1801 tcatcagaag ttagcagggc gaggactgat taaaggcaga gatcatctta tgtgggttct
     1861 cctgcaattc atttctggaa gtattcagaa aaatgcacta gctgattttc tccctgtgat
     1921 gaagctcttc gacttgctat acccagaaaa agaatatatc ccagttcctg atattaacaa
     1981 accccagtca acccatgcct ttgcaatgac ctgtatttgg attcatctca atagaaaagc
     2041 tcaaaatgac aactccaagc tacagattcc aatacctcat tccctaagac ttcaccatga
     2101 gttcctgcag cagagtctaa gaaataaaag tttacagatg aatgactata agattgctct
     2161 attgtgtaat gcatactcta caaattcaga atgttttaca ttacccatgg gagctctggt
     2221 agaaactatt tatggaaatg gaattatgag gatacctctc cctggaacaa actgtatggc
     2281 ttcaggatct attaccccct tacctatgaa cctcctggat tcactgacag ttcatgccaa
     2341 aatgagcctt attcacagca ttgcaaccag ggtgataaaa cttgctcatg caaagtccag
     2401 tgtggccttg gctccagccc tagtggaaac ttacagtcgt ttattggtct atatggaaat
     2461 agagtctttg ggcatcaaag gatttatcag tcagcttttg ccaactgttt tcaaatcaca
     2521 tgcatggggg atcttacaca cactccttga gatgtttagc taccggatgc atcatattca
     2581 gcctcattac agagttcagc tcctgagtca tcttcatact ttggctgcag ttgcacaaac
     2641 aaaccagaac cagctccatc tttgtgtcga gagcactgct ctcaggctta taacagcatt
     2701 aggtagctca gaggtacaac cgcagtttac acgcttcctt agtgatccca aaacagtgct
     2761 ctcagcagaa tctgaagaac tgaaccgagc cttgatattg accttggcta gagcaactca
     2821 tgtaacagat ttttttacag gctctgattc aattcaggga acttggtgta aagacatact
     2881 tcagaccatc atgagtttca ctcctcataa ttgggcttca cacaccctga gctgttttcc
     2941 aggtaagtat caaggttaca agacaggttt aaggagacca ataagctatt attcgtatag
     3001 gatactttat acgaagttat taagttatgg agaccggtgt ggcacgtgcg gaccgagcgg
     3061 ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact
     3121 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc
     3181 gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg
     3241 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat
     3301 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag
     3361 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     3421 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     3481 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     3541 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     3601 caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
     3661 tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     3721 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     3781 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     3841 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     3901 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     3961 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     4021 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     4081 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     4141 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     4201 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     4261 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     4321 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     4381 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     4441 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     4501 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     4561 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     4621 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     4681 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     4741 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     4801 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     4861 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     4921 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     4981 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     5041 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5101 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5161 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5221 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5281 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     5341 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5401 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5461 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5521 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5581 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5641 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5701 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5761 ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//