pSTAT3-mEGFP-iPAK4

LOCUS       pSTAT3-mEGFP-iPAK4             6503 bp DNA     circular SYN 16-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3600..4676
                     /mol_type="genomic DNA"
                     /organism="."
     polyA_signal    285..406
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(455..471)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    479..495
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(503..533)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    548..569
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    798..1468
                     /label=pUC ori
     rep_origin      complement(857..1442)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1613..2473)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2474..2578)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2497..2515
                     /label=Amp-pro-R
     misc_feature    2641..2741
                     /label=4x STAT binding
     misc_feature    2641..2655
                     /label=STAT3 binding motif
     misc_feature    2665..2684
                     /label=STAT3 binding motif
     misc_feature    2690..2709
                     /label=STAT3 binding motif
     misc_feature    2693..2701
                     /label=TTCNNNgAA
     misc_feature    2715..2734
                     /label=STAT3 binding motif
     misc_feature    2742..2786
                     /label=P_CMVmin
     promoter        2747..2785
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        2830..2848
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2855..2897
                     /label=EcoRI-mEGFP-F
     regulatory      2877..2886
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2883..3599
                     /codon_start=1
                     /label=mEGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3485..3519)
                     /label=mEGFP-Qch-R
     primer_bind     3485..3519
                     /label=mEGFP-Qch-F
     misc_feature    3501..3503
                     /label=A to K for mEGFP
     primer_bind     3578..3625
                     /label=GFP-iPAK4-F
     primer_bind     3578..3625
                     /label=Primer 1a
     CDS             3612..3725
                     /codon_start=1
                     /label=INKA-box
                     /translation="EAEDWTAALLNRGRSRQPLVLGDNCFADLVHNWMELPE"
     CDS             3732..4673
                     /codon_start=1
                     /label=Pak4cat
                     /translation="PAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLD
                     NFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEM
                     YNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRD
                     IKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIW
                     SLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPA
                     QRATAAELLKHPFLAKAGPPASIVPLMRQNRTR"
     primer_bind     complement(3825..3843)
                     /label=PAK4-R
     primer_bind     complement(4656..4698)
                     /label=HindIII-iPAK4-R
     primer_bind     complement(4706..4723)
                     /label=PcDNA3.1R( BGH )
     polyA_signal    4712..4936
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4816..4835)
                     /label=BGH-New
     rep_origin      4982..5410
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5424..5753
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5604..5739
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(5820..6503,1..111)
                     /label=NeoR
ORIGIN
        1 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
       61 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
      121 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
      181 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
      241 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
      301 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
      361 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
      421 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      481 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      541 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
      601 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
      661 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      721 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
      781 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      841 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
      901 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      961 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1021 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1081 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1141 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1201 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1261 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1321 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1381 ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1441 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1501 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     1561 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     1621 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1681 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1741 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1801 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1861 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     1921 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     1981 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2041 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2101 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2161 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     2221 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     2281 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     2341 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     2401 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     2461 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     2521 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     2581 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctccac gcgtctcgag
     2641 atttcccgta aatcgtcgag tggacatttc ccgtaaatcg tcgagtggac atttcccgta
     2701 aatcgtcgag tggacatttc ccgtaaatcg tcgagctgca gtcgagggta ggcgtgtacg
     2761 gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg cttactggct
     2821 tatcgaaatt aatacgactc actataggga gacccaagct gttcgaagcg gaattcgcca
     2881 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     2941 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     3001 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     3061 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     3121 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     3181 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     3241 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     3301 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     3361 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     3421 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     3481 actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     3541 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     3601 caggtggatc cgaagcagag gactggacag cagccctact gaacaggggt cgcagtcgcc
     3661 agcccctggt actaggggac aattgctttg ctgacttggt gcacaactgg atggagctgc
     3721 ctgaggaatt ccccgccgcc cctgctgttc ctgggccccc tggcccccgc tcaccacagc
     3781 gggagccaca gcgagtatcc catgagcagt tccgggctgc cctgcagctg gtggtggacc
     3841 caggcgaccc ccgctcctac ctggacaact tcatcaagat tggcgagggc tccacgggca
     3901 tcgtgtgcat cgccaccgtg cgcagctcgg gcaagctggt ggccgtcaag aagatggacc
     3961 tgcgcaagca gcagaggcgc gagctgctct tcaacgaggt ggtaatcatg agggactacc
     4021 agcacgagaa tgtggtggag atgtacaaca gctacctggt gggggacgag ctctgggtgg
     4081 tcatggagtt cctggaagga ggcgccctca ccgacatcgt cacccacacc aggatgaacg
     4141 aggagcagat cgcagccgtg tgccttgcag tgctgcaggc cctgtcggtg ctccacgccc
     4201 agggcgtcat ccaccgggac atcaagagcg actcgatcct gctgacccat gatggcaggg
     4261 tgaagctgtc agactttggg ttctgcgccc aggtgagcaa ggaagtgccc cgaaggaagt
     4321 cgctggtcgg cacgccctac tggatggccc cagagctcat ctcccgcctt ccctacgggc
     4381 cagaggtaga catctggtcg ctggggataa tggtgattga gatggtggac ggagagcccc
     4441 cctacttcaa cgagccaccc ctcaaagcca tgaagatgat tcgggacaac ctgccacccc
     4501 gactgaagaa cctgcacaag gtgtcgccat ccctgaaggg cttcctggac cgcctgctgg
     4561 tgcgagaccc tgcccagcgg gccacggcag ccgagctgct gaagcaccca ttcctggcca
     4621 aggcagggcc gcctgccagc atcgtgcccc tcatgcgcca gaaccgcacc agatgaaagc
     4681 tttgaggccg gcctgcaggg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     4741 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     4801 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     4861 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     4921 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     4981 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5041 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     5101 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     5161 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     5221 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     5281 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     5341 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     5401 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     5461 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     5521 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     5581 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     5641 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     5701 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     5761 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca
     5821 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     5881 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     5941 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     6001 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     6061 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     6121 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     6181 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     6241 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     6301 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     6361 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     6421 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     6481 tagcgttggc tacccgtgat att
//