| 质粒名称: |
pAAVLINK-EF1a-HA-5'-MTOR
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001695 |
| 产品用途: | 5' AAVLINK plasmid for MTOR expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | MTOR |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-MTOR
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-MTOR载体质粒基本信息
pAAVLINK-EF1a-HA-5'-MTOR质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-MTOR载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-MTOR质粒载体简介
pAAVLINK-EF1a-HA-5'-MTOR质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-MTOR 7259 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7259)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 2, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7259
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..4399
/codon_start=1
/label=MTOR
/translation="LGTGPAAATTAATTSSNVSVLQQFASGLKSRNEETRAKAAKELQH
YVTMELREMSQEESTRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIG
RFANYLRNLLPSNDPVVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGR
RHAAVLVLRELAISVPTFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQR
EPKEMQKPQWYRHTFEEAEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERL
REEMEEITQQQLVHDKYCKDLMGFGTKPRHITPFTSFQAVQPQQSNALVGLLGYSSHQG
LMGFGTSPSPAKSTLVESRCCRDLMEEKFDQVCQWVLKCRNSKNSLIQMTILNLLPRLA
AFRPSAFTDTQYLQDTMNHVLSCVKKEKERTAAFQALGLLSVAVRSEFKVYLPRVLDII
RAALPPKDFAHKRQKAMQVDATVFTCISMLARAMGPGIQQDIKELLEPMLAVGLSPALT
AVLYDLSRQIPQLKKDIQDGLLKMLSLVLMHKPLRHPGMPKGLAHQLASPGLTTLPEAS
DVGSITLALRTLGSFEFEGHSLTQFVRHCADHFLNSEHKEIRMEAARTCSRLLTPSIHL
ISGHAHVVSQTAVQVVADVLSKLLVVGITDPDPDIRYCVLASLDERFDAHLAQAENLQA
LFVALNDQVFEIRELAICTVGRLSSMNPAFVMPFLRKMLIQILTELEHSGIGRIKEQSA
RMLGHLVSNAPRLIRPYMEPILKALILKLKDPDPDPNPGVINNVLATIGELAQVSGLEM
RKWVDELFIIIMDMLQDSSLLAKRQVALWTLGQLVASTGYVVEPYRKYPTLLEVLLNFL
KTEQNQGTRREAIRVLGLLGALDPYKHKVNIGMIDQSRDASAVSLSESKSSQDSSDYST
SEMLVNMGNLPLDEFYPAVSMVALMRIFRDQSLSHHHTMVVQAITFIFKSLGLKCVQFL
PQVMPTFLNVIRVCDGAIREFLFQQLGMLVSFVKSHIRPYMDEIVTLMREFWVMNTSIQ
STIILLIEQIVVALGGEFKLYLPQLIPHMLRVFMHDNSPGRIVSIKLLAAIQLFGANLD
DYLHLLLPPIVKLFDAPEAPLPSRKAALETVDRLTESLDFTDYASRIIHPIVRTLDQSP
ELRSTAMDTLSSLVFQLGKKYQIFIPMVNKVLVRHRINHQRYDVLICRIVK"
misc_feature 1084..1089
/label=SDS
regulatory 3597..3606
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_feature 4091..4096
/label=SDS
intron 4400..4440
/label=Splicing Donor
misc_feature 4444..4477
/label=lox2272-JT1571
repeat_region 4522..4662
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4522..4651
/label=AAV2 ITR
rep_origin 4737..5192
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5474..5578
/gene="bla"
/label=AmpR promoter
CDS 5579..6439
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6610..7198
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctct tggaaccgga cctgccgccg ccaccaccgc
781 tgccaccaca tctagcaatg tgagcgtcct gcagcagttt gccagtggcc taaagagccg
841 gaatgaggaa accagggcca aagccgccaa ggagctccag cactatgtca ccatggaact
901 ccgagagatg agtcaagagg agtctactcg cttctatgac caactgaacc atcacatttt
961 tgaattggtt tccagctcag atgccaatga gaggaaaggt ggcatcttgg ccatagctag
1021 cctcatagga gtggaaggtg ggaatgccac ccgaattggc agatttgcca actatcttcg
1081 gaacctcctc ccctccaatg acccagttgt catggaaatg gcatccaagg ccattggccg
1141 tcttgccatg gcaggggaca cttttaccgc tgagtacgtg gaatttgagg tgaagcgagc
1201 cctggaatgg ctgggtgctg accgcaatga gggccggaga catgcagctg tcctggttct
1261 ccgtgagctg gccatcagcg tccctacctt cttcttccag caagtgcaac ccttctttga
1321 caacattttt gtggccgtgt gggaccccaa acaggccatc cgtgagggag ctgtagccgc
1381 ccttcgtgcc tgtctgattc tcacaaccca gcgtgagccg aaggagatgc agaagcctca
1441 gtggtacagg cacacatttg aagaagcaga gaagggattt gatgagacct tggccaaaga
1501 gaagggcatg aatcgggatg atcggatcca tggagccttg ttgatcctta acgagctggt
1561 ccgaatcagc agcatggagg gagagcgtct gagagaagaa atggaagaaa tcacacagca
1621 gcagctggta cacgacaagt actgcaaaga tctcatgggc ttcggaacaa aacctcgtca
1681 cattaccccc ttcaccagtt tccaggctgt acagccccag cagtcaaatg ccttggtggg
1741 gctgctgggg tacagctctc accaaggcct catgggattt gggacctccc ccagtccagc
1801 taagtccacc ctggtggaga gccggtgttg cagagacttg atggaggaga aatttgatca
1861 ggtgtgccag tgggtgctga aatgcaggaa tagcaagaac tcgctgatcc aaatgacaat
1921 ccttaatttg ttgccccgct tggctgcatt ccgaccttct gccttcacag atacccagta
1981 tctccaagat accatgaacc atgtcctaag ctgtgtcaag aaggagaagg aacgtacagc
2041 ggccttccaa gccctggggc tactttctgt ggctgtgagg tctgagttta aggtctattt
2101 gcctcgcgtg ctggacatca tccgagcggc cctgccccca aaggacttcg cccataagag
2161 gcagaaggca atgcaggtgg atgccacagt cttcacttgc atcagcatgc tggctcgagc
2221 aatggggcca ggcatccagc aggatatcaa ggagctgctg gagcccatgc tggcagtggg
2281 actaagccct gccctcactg cagtgctcta cgacctgagc cgtcagattc cacagctaaa
2341 gaaggacatt caagatgggc tactgaaaat gctgtccctg gtccttatgc acaaacccct
2401 tcgccaccca ggcatgccca agggcctggc ccatcagctg gcctctcctg gcctcacgac
2461 cctccctgag gccagcgatg tgggcagcat cactcttgcc ctccgaacgc ttggcagctt
2521 tgaatttgaa ggccactctc tgacccaatt tgttcgccac tgtgcggatc atttcctgaa
2581 cagtgagcac aaggagatcc gcatggaggc tgcccgcacc tgctcccgcc tgctcacacc
2641 ctccatccac ctcatcagtg gccatgctca tgtggttagc cagaccgcag tgcaagtggt
2701 ggcagatgtg cttagcaaac tgctcgtagt tgggataaca gatcctgacc ctgacattcg
2761 ctactgtgtc ttggcgtccc tggacgagcg ctttgatgca cacctggccc aggcggagaa
2821 cttgcaggcc ttgtttgtgg ctctgaatga ccaggtgttt gagatccggg agctggccat
2881 ctgcactgtg ggccgactca gtagcatgaa ccctgccttt gtcatgcctt tcctgcgcaa
2941 gatgctcatc cagattttga cagagttgga gcacagtggg attggaagaa tcaaagagca
3001 gagtgcccgc atgctggggc acctggtctc caatgccccc cgactcatcc gcccctacat
3061 ggagcctatt ctgaaggcat taattttgaa actgaaagat ccagaccctg atccaaaccc
3121 aggtgtgatc aataatgtcc tggcaacaat aggagaattg gcacaggtta gtggcctgga
3181 aatgaggaaa tgggttgatg aactttttat tatcatcatg gacatgctcc aggattcctc
3241 tttgttggcc aaaaggcagg tggctctgtg gaccctggga cagttggtgg ccagcactgg
3301 ctatgtagta gagccctaca ggaagtaccc tactttgctt gaggtgctac tgaattttct
3361 gaagactgag cagaaccagg gtacacgcag agaggccatc cgtgtgttag ggcttttagg
3421 ggctttggat ccttacaagc acaaagtgaa cattggcatg atagaccagt cccgggatgc
3481 ctctgctgtc agcctgtcag aatccaagtc aagtcaggat tcctctgact atagcactag
3541 tgaaatgctg gtcaacatgg gaaacttgcc tctggatgag ttctacccag ctgtgtccat
3601 ggtggccctg atgcggatct tccgagacca gtcactctct catcatcaca ccatggttgt
3661 ccaggccatc accttcatct tcaagtccct gggactcaaa tgtgtgcagt tcctgcccca
3721 ggtcatgccc acgttcctta acgtcattcg agtctgtgat ggggccatcc gggaattttt
3781 gttccagcag ctgggaatgt tggtgtcctt tgtgaagagc cacatcagac cttatatgga
3841 tgaaatagtc accctcatga gagaattctg ggtcatgaac acctcaattc agagcacgat
3901 cattcttctc attgagcaaa ttgtggtagc tcttgggggt gaatttaagc tctacctgcc
3961 ccagctgatc ccacacatgc tgcgtgtctt catgcatgac aacagcccag gccgcattgt
4021 ctctatcaag ttactggctg caatccagct gtttggcgcc aacctggatg actacctgca
4081 tttactgctg cctcctattg ttaagttgtt tgatgcccct gaagctccac tgccatctcg
4141 aaaggcagcg ctagagactg tggaccgcct gacggagtcc ctggatttca ctgactatgc
4201 ctcccggatc attcacccta ttgttcgaac actggaccag agcccagaac tgcgctccac
4261 agccatggac acgctgtctt cacttgtttt tcagctgggg aagaagtacc aaattttcat
4321 tccaatggtg aataaagttc tggtgcgaca ccgaatcaat catcagcgct atgatgtgct
4381 catctgcaga attgtcaagg taagtatcaa ggttacaaga caggtttaag gagaccaata
4441 agctattatt cgtataggat actttatacg aagttattaa gttatggaga ccggtgtggc
4501 acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
4561 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
4621 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt
4681 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
4741 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
4801 acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
4861 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
4921 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
4981 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
5041 cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg
5101 gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc
5161 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
5221 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
5281 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
5341 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
5401 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
5461 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
5521 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
5581 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
5641 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
5701 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
5761 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
5821 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
5881 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
5941 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
6001 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
6061 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
6121 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
6181 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
6241 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
6301 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
6361 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
6421 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
6481 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
6541 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
6601 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
6661 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
6721 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
6781 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
6841 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
6901 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
6961 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
7021 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
7081 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
7141 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
7201 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
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