pLZ287

LOCUS      pLZ287              4040 bp DNA     circular SYN 02-APR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 19, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..4040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    653..787
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1132..1760)
                     /direction=LEFT
                     /label=ColE1 origin
     rep_origin      complement(1150..1738)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2207..2585)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(2770..2874)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(2811..2839)
                     /label=Amp prom
     misc_feature    2906..2955
                     /label=2XSTAT3
     misc_feature    2968..3017
                     /label=2XSTAT3
     misc_feature    3024..3073
                     /label=2XSTAT3
     misc_feature    3080..3129
                     /label=2XSTAT3
     misc_feature    3144..3292
                     /label=P-hCMVmin
     promoter        3166..3204
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3277..3292)
                     /label=oYW11
     primer_bind     3303..3350
                     /label=oLH075 mCherry F
     primer_bind     3323..3355
                     /label=olyl129
     CDS             3330..4039
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVARARRITWPSSRSSCASRCTWRAP*TATSSRSRARARAAPTRA
                     PRPPS*R*PRVAPCPSPGTSCPLSSCTAPRPT*STPPTSPTT*SCPSPRASSGSA**TS
                     RTAAW*P*PRTPPCRTASSSTR*SCAAPTSPPTAP*CRRRPWAGRPPPSGCTPRTAP*R
                     ARSSRG*S*RTAATTTLRSRPPTRPRSPCSCPAPTTSTSSWTSPPTTRTTPSWNSTNAP
                     RAATPPAAWTSCTS"
     CDS             3330
                     /codon_start=1
                     /product="start codon"
                     /label=ATG
                     /translation=""
     primer_bind     3336..3355
                     /label=olyl674
     primer_bind     3336..3349
                     /label=mCherry-1
     primer_bind     complement(join(4016..4040,1..19))
                     /label=oLH076 mCherry R
     primer_bind     complement(4017..4039)
                     /label=OLYL661
ORIGIN
        1 agcttttaaa acagctctgg ggttgtaccc accccagagg cccacgtggc ggctagtact
       61 ccggtattgc ggtacccttg tacgcctgtt ttatactccc ttcccgtaac ttagacgcac
      121 aaaaccaagt tcaatagaag ggggtacaaa ccagtaccac cacgaacaag cacttctgtt
      181 tccccggtga tgtcgtatag actgcttgcg tggttgaaag cgacggatcc gttatccgct
      241 tatgtacttc gagaagccca gtaccacctc ggaatcttcg atgcgttgcg ctcagcactc
      301 aaccccagag tgtagcttag gctgatgagt ctggacatcc ctcaccggtg acggtggtcc
      361 aggctgcgtt ggcggcctac ctatggctaa cgccatggga cgctagttgt gaacaaggtg
      421 tgaagagcct attgagctac ataagaatcc tccggcccct gaatgcggct aatcccaacc
      481 tcggagcagg tggtcacaaa ccagtgattg gcctgtcgta acgcgcaagt ccgtggcgga
      541 accgactact ttgggtgtcc gtgtttcctt ttattttatt gtggctgctt atggtgacaa
      601 tcacagattg ttatcataaa gcgaattgga ttgcggccgc aaagctagct tcgatccaga
      661 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
      721 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
      781 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
      841 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atccggctgc
      901 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
      961 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     1021 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
     1081 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatc gggccgcgtt
     1141 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1201 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1261 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1321 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1381 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1441 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1501 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1561 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     1621 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1681 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1741 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1801 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1861 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     1921 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     1981 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     2041 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     2101 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     2161 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     2221 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     2281 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     2341 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     2401 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     2461 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     2521 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     2581 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     2641 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     2701 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     2761 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     2821 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     2881 tccccgaaaa gtgccacctg acgtcgtcga catttcccgt aaatcgtcga gtcgacattt
     2941 cccgtaaatc gtcgagggcc catcgatgtc gacatttccc gtaaatcgtc gagtcgacat
     3001 ttcccgtaaa tcgtcgaggg cccgtcgaca tttcccgtaa atcgtcgagt cgacatttcc
     3061 cgtaaatcgt cgaatcgatg tcgacatttc ccgtaaatcg tcgagtcgac atttcccgta
     3121 aatcgtcgaa cgcgtcctgc aggtcgagct cggtacccgg gtcgaggtag gcgtgtacgg
     3181 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
     3241 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggaattcga
     3301 gctcgcccgg ggatcctcta gagccaccta tggtggcaag ggcgaggagg ataacatggc
     3361 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
     3421 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     3481 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     3541 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
     3601 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     3661 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     3721 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     3781 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
     3841 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
     3901 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     3961 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     4021 ggacgagctg tacaagtaaa
//