| 质粒名称: | pLZ413 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000628 |
| 产品用途: | Mammalian VanR-specific GEMSANR expression vector (VanO5-PhCMVmin-GEMSANR-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: |
pCMV
|
| 表达系统: | 哺乳动物表达 |
| 启动子: |
VanO5-PhCMVmin
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | GEMSANR |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Multi-layered computational gene networks by engineered tristate logics
Shao J., Qiu X., Zhang L., Li S., Xue S., Si Y., Li Y., Jiang J., Wu Y., Xiong Q., Wang Y., Chen Q., Gao T., Zhu L., Wang H., Xie M.
Cell 187, 5064-5080.e14 (2024)
|
pLZ413
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pLZ413载体质粒基本信息
pLZ413质粒图谱载体图谱和pLZ413载体序列质粒序列多克隆位点信息
pLZ413质粒载体简介
pLZ413质粒序列载体序列
LOCUS pLZ413 7709 bp DNA circular SYN 10-DEC-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7709)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Jul 20, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7709
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 228
/label=E2F-1
misc_feature 232
/label=CMV
misc_feature 234..245
/label=VanO
/label=nonstandard type: operator
misc_feature 248..259
/label=VanO
/label=nonstandard type: operator
misc_feature 262..273
/label=VanO
/label=nonstandard type: operator
misc_feature 276..287
/label=VanO
/label=nonstandard type: operator
misc_feature 290..301
/label=VanO
/label=nonstandard type: operator
misc_feature 302..306
/label=E2F-1
misc_feature 307..455
/label=hcmvmin
promoter 329..367
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 377..391
/label=New Feature
primer_bind 436..456
/label=181-WKG-593-F
regulatory 461..470
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_feature 467..529
/label=Igk secretion signal
regulatory 542..551
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 550
/codon_start=1
/label=5xGS Linker
/translation=""
CDS 551..613
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 614..688
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 689..751
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 752..826
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 833..895
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 896..970
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 971..1033
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1034..1108
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1115..1177
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1178..1252
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1253..1315
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1316..1390
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1397..1459
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1460..1534
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1535..1597
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1598..1672
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
misc_feature 1685..2428
/label=mouse EpoR-D1_D2_TM
/note="mouse EpoR-D1_D2_TM"
primer_bind complement(1752..1774)
/label=186-WKG-603-R
misc_feature 1958..1960
/label=F93A
/note="F93A"
misc_feature 2412..2428
/label=EpoR-D1_D2_TM
misc_feature 2438..3266
/label=IL6ST
misc_feature 2465..2491
/label=box1
misc_feature 2789..2791
/label=Y759A
polyA_signal 3309..3533
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3579..4007
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3593..3617)
/label=184-WKG-599-R
promoter 4021..4350
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 4417..5211
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 5385..5506
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5551..5571)
/label=M13-rev
primer_bind complement(5555..5571)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_binding complement(5577..5599)
/label=LacO
protein_bind 5579..5595
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5603..5633)
/label=lac promoter
/note="promoter for the E. coli lac operon"
promoter complement(5604..5633)
/label=lac
protein_bind 5648..5669
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 5775..6712
/label=ColE1
/note="ColE1"
CDS complement(6713..7573)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7574..7678)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtattggat
241 ccaatgcatt ggatccaatg gattggatcc aatcgattgg atccaattga ttggatccaa
301 tctagctcga gctcggtacc cgggtcgagg taggcgtgta cggtgggagg cctatataag
361 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
421 ccatagaaga caccgggacc gatccagcct ccgcgggccg gccaccatgg agaccgatac
481 cttattactg tgggtgcttc ttctgtgggt tcccggtagc accggagacg gatccaagct
541 tgccaccatg ggggagctcg acgagttggt ttatctctta gacggacccg gatatgaccc
601 cattcattca gatggctccg gaacgggcag cgggaccgga agtggcaccg gaaccacttc
661 cggaaccggt actggcggga gtaccggcgg tgagctagac gaactcgtgt acctgttgga
721 tggccccggt tacgacccaa tccacagcga tggaagtgga actgggtctg ggactgggtc
781 cggcaccggg actacttcag gcaccggcac tgggggctcc actggccata ccggggagct
841 cgacgagttg gtttatctct tagacggacc cggatatgac cccattcatt cagatggctc
901 cggaacgggc agcgggaccg gaagtggcac cggaaccact tccggaaccg gtactggcgg
961 gagtaccggc ggtgagctag acgaactcgt gtacctgttg gatggccccg gttacgaccc
1021 aatccacagc gatggaagtg gaactgggtc tgggactggg tccggcaccg ggactacttc
1081 aggcaccggc actgggggct ccactggcca taccggggag ctcgacgagt tggtttatct
1141 cttagacgga cccggatatg accccattca ttcagatggc tccggaacgg gcagcgggac
1201 cggaagtggc accggaacca cttccggaac cggtactggc gggagtaccg gcggtgagct
1261 agacgaactc gtgtacctgt tggatggccc cggttacgac ccaatccaca gcgatggaag
1321 tggaactggg tctgggactg ggtccggcac cgggactact tcaggcaccg gcactggggg
1381 ctccactggc cataccgggg agctcgacga gttggtttat ctcttagacg gacccggata
1441 tgaccccatt cattcagatg gctccggaac gggcagcggg accggaagtg gcaccggaac
1501 cacttccgga accggtactg gcgggagtac cggcggtgag ctagacgaac tcgtgtacct
1561 gttggatggc cccggttacg acccaatcca cagcgatgga agtggaactg ggtctgggac
1621 tgggtccggc accgggacta cttcaggcac cggcactggg ggctccactg gccatatgga
1681 attcgcacct tcacccagcc tcccggaccc caagtttgag agcaaagcgg ccctgctggc
1741 atcccggggc tccgaagaac ttctgtgctt cacccaacgc ttggaagact tggtgtgttt
1801 ctgggaggaa gcggcgagct ccgggatgga cttcaactac agcttctcat accagctcga
1861 gggtgagtca cgaaagtcat gtagcctgca ccaggctccc accgtccgcg gctccgtgcg
1921 tttctggtgt tcactgccaa cagcggacac atcgagtgct gtgccgctgg agctgcaggt
1981 gacggaggcg tccggttctc ctcgctatca ccgcatcatc catatcaatg aagtagtgct
2041 cctggacgcc cccgcggggc tgctggcgcg ccgggcagaa gagggcagcc acgtggtgct
2101 gcgctggctg ccacctcctg gagcacctat gaccacccac atccgatatg aagtggacgt
2161 gtcggcaggc aaccgggcag gagggacaca aagggtggag gtcctggaag gccgcactga
2221 gtgtgttctg agcaacctgc ggggcgggac gcgctacacc ttcgctgttc gagcgcgcat
2281 ggccgagccg agcttcagcg gattctggag tgcctggtct gagcccgcgt cactactgac
2341 cgctagcgac ctggaccctc tcatcttgac gctgtctctc attctggtcc tcatctcgtt
2401 gttgctgacg gttctggccc tgctgtccgc agcagcaaat aagcgagacc taattaaaaa
2461 acacatctgg cctaatgttc cagatccttc aaagagtcat attgcccagt ggtcacctca
2521 cactcctcca aggcacaatt ttaattcaaa agatcaaatg tattcagatg gcaatttcac
2581 tgatgtaagt gttgtggaaa tagaagcaaa tgacaaaaag ccttttccag aagatctgaa
2641 atcattggac ctgttcaaaa aggaaaaaat taatactgaa ggacacagca gtggtattgg
2701 ggggtcttca tgcatgtcat cttctaggcc aagcatttct agcagtgatg aaaatgaatc
2761 ttcacaaaac acttcgagca ctgtccaggc ttctaccgtg gtacacagtg gctacagaca
2821 ccaagttccg tcagtccaag tcttctcaag atccgagtct acccagccct tgttagattc
2881 agaggagcgg ccagaagatc tacaattagt agatcatgta gatggcggtg atggtatttt
2941 tcccaggcaa cagtacttca aacagaactg cagtcagcat gaatccagtc cagatatttc
3001 acattttgaa aggtcaaagc aagtttcatc agtcaatgag gaagattttg ttagacttaa
3061 acagcagatt tcagatcata tttcacaatc ctgtggatct gggcaaatga aaatgtttca
3121 ggaagtttct gcagcagatg cttttggtcc aggtactgag ggacaagtag aaagatttga
3181 aacagttggc atggaggctg cgactgatga aggcatgcct aaaagttact taccacagac
3241 tgtacggcaa ggcggctaca tgcctcagta atctagaggg cccgtttaaa cccgctgatc
3301 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
3361 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
3421 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
3481 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
3541 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
3601 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
3661 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
3721 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
3781 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
3841 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
3901 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
3961 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
4021 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
4081 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
4141 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
4201 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
4261 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
4321 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
4381 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
4441 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
4501 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
4561 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
4621 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
4681 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
4741 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
4801 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
4861 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
4921 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
4981 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
5041 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
5101 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
5161 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
5221 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
5281 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
5341 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
5401 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
5461 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
5521 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
5581 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
5641 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
5701 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
5761 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
5821 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
5881 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
5941 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
6001 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
6061 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
6121 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
6181 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
6241 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
6301 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
6361 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
6421 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
6481 ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
6541 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
6601 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
6661 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
6721 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
6781 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
6841 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
6901 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
6961 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
7021 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
7081 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
7141 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
7201 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
7261 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
7321 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
7381 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
7441 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
7501 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
7561 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
7621 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
7681 catttccccg aaaagtgcca cctgacgtc
//
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