| 质粒名称: | pLZ268 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000604 |
| 产品用途: | Mammalian PSV40-driven constitutive GEMSANR expression vector (PSV40-GEMSANR-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | SV40 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | GEMSANR |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Multi-layered computational gene networks by engineered tristate logics
Shao J., Qiu X., Zhang L., Li S., Xue S., Si Y., Li Y., Jiang J., Wu Y., Xiong Q., Wang Y., Chen Q., Gao T., Zhu L., Wang H., Xie M.
Cell 187, 5064-5080.e14 (2024)
|
pLZ268
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pLZ268载体质粒基本信息
pLZ268质粒图谱载体图谱和pLZ268载体序列质粒序列多克隆位点信息
pLZ268质粒载体简介
pLZ268质粒序列载体序列
LOCUS pLZ268 7718 bp DNA circular SYN 13-JAN-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7718)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Jul 18, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7718
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 166..191
/label=182-WKG-596-F
misc_feature 232
/label=CMV
promoter 245..441
/label=SV40 promoter
/note="SV40 early promoter"
regulatory 467..476
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_feature 473..535
/label=Igk secretion signal
regulatory 548..557
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 556
/codon_start=1
/label=5xGS Linker
/translation=""
CDS 557..619
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 620..694
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 695..757
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 758..832
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 839..901
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 902..976
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 977..1039
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1040..1114
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1121..1183
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1184..1258
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1259..1321
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1322..1396
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1403..1465
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1466..1540
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1541..1603
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1604..1678
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
misc_feature 1691..2434
/label=mouse EpoR-D1_D2_TM
/note="mouse EpoR-D1_D2_TM"
primer_bind complement(1758..1780)
/label=186-WKG-603-R
misc_feature 1964..1966
/label=F93A
/note="F93A"
misc_feature 2418..2434
/label=EpoR-D1_D2_TM
misc_feature 2444..3272
/label=IL6ST
misc_feature 2471..2497
/label=box1
misc_feature 2795..2797
/label=Y759A
polyA_signal 3315..3539
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3585..4013
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3599..3623)
/label=184-WKG-599-R
promoter 4027..4356
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 4423..5217
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 5391..5512
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5557..5577)
/label=M13-rev
primer_bind complement(5561..5577)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_binding complement(5583..5605)
/label=LacO
protein_bind 5585..5601
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5609..5639)
/label=lac promoter
/note="promoter for the E. coli lac operon"
promoter complement(5610..5639)
/label=lac
protein_bind 5654..5675
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 5781..6721
/label=ColE1
/note="ColE1"
CDS complement(6722..7582)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7583..7687)
/gene="bla"
/label=AmpR promoter
promoter complement(7624..7652)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgatctgc
241 gatctgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
301 ctaactccgc ccagttccgc ccattctccg ccccatcgct gactaatttt ttttatttat
361 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
421 ggaggcctag gcttttgcaa aaagctacga atcgcgctag tggccggcca ccatggagac
481 cgatacctta ttactgtggg tgcttcttct gtgggttccc ggtagcaccg gagacggatc
541 caagcttgcc accatggggg agctcgacga gttggtttat ctcttagacg gacccggata
601 tgaccccatt cattcagatg gctccggaac gggcagcggg accggaagtg gcaccggaac
661 cacttccgga accggtactg gcgggagtac cggcggtgag ctagacgaac tcgtgtacct
721 gttggatggc cccggttacg acccaatcca cagcgatgga agtggaactg ggtctgggac
781 tgggtccggc accgggacta cttcaggcac cggcactggg ggctccactg gccataccgg
841 ggagctcgac gagttggttt atctcttaga cggacccgga tatgacccca ttcattcaga
901 tggctccgga acgggcagcg ggaccggaag tggcaccgga accacttccg gaaccggtac
961 tggcgggagt accggcggtg agctagacga actcgtgtac ctgttggatg gccccggtta
1021 cgacccaatc cacagcgatg gaagtggaac tgggtctggg actgggtccg gcaccgggac
1081 tacttcaggc accggcactg ggggctccac tggccatacc ggggagctcg acgagttggt
1141 ttatctctta gacggacccg gatatgaccc cattcattca gatggctccg gaacgggcag
1201 cgggaccgga agtggcaccg gaaccacttc cggaaccggt actggcggga gtaccggcgg
1261 tgagctagac gaactcgtgt acctgttgga tggccccggt tacgacccaa tccacagcga
1321 tggaagtgga actgggtctg ggactgggtc cggcaccggg actacttcag gcaccggcac
1381 tgggggctcc actggccata ccggggagct cgacgagttg gtttatctct tagacggacc
1441 cggatatgac cccattcatt cagatggctc cggaacgggc agcgggaccg gaagtggcac
1501 cggaaccact tccggaaccg gtactggcgg gagtaccggc ggtgagctag acgaactcgt
1561 gtacctgttg gatggccccg gttacgaccc aatccacagc gatggaagtg gaactgggtc
1621 tgggactggg tccggcaccg ggactacttc aggcaccggc actgggggct ccactggcca
1681 tatggaattc gcaccttcac ccagcctccc ggaccccaag tttgagagca aagcggccct
1741 gctggcatcc cggggctccg aagaacttct gtgcttcacc caacgcttgg aagacttggt
1801 gtgtttctgg gaggaagcgg cgagctccgg gatggacttc aactacagct tctcatacca
1861 gctcgagggt gagtcacgaa agtcatgtag cctgcaccag gctcccaccg tccgcggctc
1921 cgtgcgtttc tggtgttcac tgccaacagc ggacacatcg agtgctgtgc cgctggagct
1981 gcaggtgacg gaggcgtccg gttctcctcg ctatcaccgc atcatccata tcaatgaagt
2041 agtgctcctg gacgcccccg cggggctgct ggcgcgccgg gcagaagagg gcagccacgt
2101 ggtgctgcgc tggctgccac ctcctggagc acctatgacc acccacatcc gatatgaagt
2161 ggacgtgtcg gcaggcaacc gggcaggagg gacacaaagg gtggaggtcc tggaaggccg
2221 cactgagtgt gttctgagca acctgcgggg cgggacgcgc tacaccttcg ctgttcgagc
2281 gcgcatggcc gagccgagct tcagcggatt ctggagtgcc tggtctgagc ccgcgtcact
2341 actgaccgct agcgacctgg accctctcat cttgacgctg tctctcattc tggtcctcat
2401 ctcgttgttg ctgacggttc tggccctgct gtccgcagca gcaaataagc gagacctaat
2461 taaaaaacac atctggccta atgttccaga tccttcaaag agtcatattg cccagtggtc
2521 acctcacact cctccaaggc acaattttaa ttcaaaagat caaatgtatt cagatggcaa
2581 tttcactgat gtaagtgttg tggaaataga agcaaatgac aaaaagcctt ttccagaaga
2641 tctgaaatca ttggacctgt tcaaaaagga aaaaattaat actgaaggac acagcagtgg
2701 tattgggggg tcttcatgca tgtcatcttc taggccaagc atttctagca gtgatgaaaa
2761 tgaatcttca caaaacactt cgagcactgt ccaggcttct accgtggtac acagtggcta
2821 cagacaccaa gttccgtcag tccaagtctt ctcaagatcc gagtctaccc agcccttgtt
2881 agattcagag gagcggccag aagatctaca attagtagat catgtagatg gcggtgatgg
2941 tatttttccc aggcaacagt acttcaaaca gaactgcagt cagcatgaat ccagtccaga
3001 tatttcacat tttgaaaggt caaagcaagt ttcatcagtc aatgaggaag attttgttag
3061 acttaaacag cagatttcag atcatatttc acaatcctgt ggatctgggc aaatgaaaat
3121 gtttcaggaa gtttctgcag cagatgcttt tggtccaggt actgagggac aagtagaaag
3181 atttgaaaca gttggcatgg aggctgcgac tgatgaaggc atgcctaaaa gttacttacc
3241 acagactgta cggcaaggcg gctacatgcc tcagtaatct agagggcccg tttaaacccg
3301 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
3361 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
3421 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
3481 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
3541 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
3601 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
3661 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
3721 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
3781 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
3841 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
3901 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
3961 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
4021 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
4081 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
4141 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
4201 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
4261 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
4321 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
4381 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
4441 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
4501 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
4561 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
4621 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
4681 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
4741 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
4801 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
4861 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
4921 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
4981 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
5041 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
5101 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
5161 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
5221 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
5281 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
5341 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
5401 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
5461 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
5521 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
5581 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
5641 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
5701 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
5761 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
5821 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
5881 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
5941 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
6001 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
6061 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
6121 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
6181 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
6241 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
6301 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
6361 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
6421 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
6481 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
6541 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
6601 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
6661 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
6721 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
6781 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
6841 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
6901 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
6961 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
7021 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
7081 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
7141 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
7201 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
7261 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
7321 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
7381 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
7441 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
7501 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
7561 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
7621 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
7681 ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
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