| 质粒名称: | pH2B-mTFP1-N1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000729 |
| 产品用途: | Express mTFP1 fused to C-terminus of H2B |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Top10 |
|
插入基因信息 |
|
| 插入基因名称: | H2B, mTFP1 |
| 物种来源: | Synthetic |
| 密码子优化: | 未优化 |
pH2B-mTFP1-N1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pH2B-mTFP1-N1载体质粒基本信息
pH2B-mTFP1-N1质粒图谱载体图谱和pH2B-mTFP1-N1载体序列质粒序列多克隆位点信息
pH2B-mTFP1-N1质粒载体简介
pH2B-mTFP1-N1质粒序列载体序列
LOCUS pH2B-mTFP1-N1 5091 bp DNA circular SYN 18-JUL-2024
DEFINITION Localization: Nucleus/Histones, Excitation: 383, Emission: 448.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5091)
TITLE Davidson Blue Fluorescent Proteins
REFERENCE 2 (bases 1 to 5091)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 22, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5091
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 68..371
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 372..575
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 526..546
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 664..1041
/codon_start=1
/gene="HIST1H2BJ"
/product="human histone H2B"
/label=H2B
/translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
GELAKHAVSEGTKAITKYTSAK"
primer_bind 901..921
/label=H2B-F
primer_bind 1032..1057
/label=0416-PM643-F
CDS 1054..1761
/codon_start=1
/product="monomeric teal (cyan) fluorescent protein (Ai et
al., 2006)"
/label=mTFP1
/translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
VARNSTDGMDELYK"
primer_bind 1054..1085
/label=Seq-1-TFP
primer_bind complement(1762..1787)
/label=0416-PM643-R
polyA_signal 1886..2007
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1923..1942)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1977..1996
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2014..2469)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2151..2172)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2363..2382
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2496..2600
/gene="bla"
/label=AmpR promoter
promoter 2602..2959
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2625..2645)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2810..2945
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2872..2891
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(2895..2906)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2994..3788
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3048..3067)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3658..3677
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3976..3995)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4020..4067
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4396..4984
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4885..4904
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
601 agcgctaccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcc
661 accatgccag agccagcgaa gtctgctccc gccccgaaaa agggctccaa gaaggcggtg
721 actaaggcgc agaagaaagg cggcaagaag cgcaagcgca gccgcaagga gagctattcc
781 atctatgtgt acaaggttct gaagcaggtc caccctgaca ccggcatttc gtccaaggcc
841 atgggcatca tgaattcgtt tgtgaacgac attttcgagc gcatcgcagg tgaggcttcc
901 cgcctggcgc attacaacaa gcgctcgacc atcacctcca gggagatcca gacggccgtg
961 cgcctgctgc tgcctgggga gttggccaag cacgccgtgt ccgagggtac taaggccatc
1021 accaagtaca ccagcgctaa ggatccaccg gtcatggtga gcaagggcga ggagaccaca
1081 atgggcgtaa tcaagcccga catgaagatc aagctgaaga tggagggcaa cgtgaatggc
1141 cacgccttcg tgatcgaggg cgagggcgag ggcaagccct acgacggcac caacaccatc
1201 aacctggagg tgaaggaggg agcccccctg cccttctcct acgacattct gaccaccgcg
1261 ttcgcctacg gcaacagggc cttcaccaag taccccgacg acatccccaa ctacttcaag
1321 cagtccttcc ccgagggcta ctcttgggag cgcaccatga ccttcgagga caagggcatc
1381 gtgaaggtga agtccgacat ctccatggag gaggactcct tcatctacga gatacacctc
1441 aagggcgaga acttcccccc caacggcccc gtgatgcaga agaagaccac cggctgggac
1501 gcctccaccg agaggatgta cgtgcgcgac ggcgtgctga agggcgacgt caagcacaag
1561 ctgctgctgg agggcggcgg ccaccaccgc gttgacttca agaccatcta cagggccaag
1621 aaggcggtga agctgcccga ctatcacttt gtggaccacc gcatcgagat cctgaaccac
1681 gacaaggact acaacaaggt gaccgtttac gagagcgccg tggcccgcaa ctccaccgac
1741 ggcatggacg agctgtacaa gtaggcggcc gcgactctag atcataatca gccataccac
1801 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
1861 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
1921 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
1981 tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa
2041 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
2101 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
2161 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
2221 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
2281 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
2341 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
2401 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
2461 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
2521 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
2581 aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
2641 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
2701 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
2761 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
2821 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
2881 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
2941 aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg
3001 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
3061 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
3121 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
3181 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
3241 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
3301 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
3361 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
3421 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
3481 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
3541 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
3601 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
3661 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
3721 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
3781 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
3841 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
3901 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
3961 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
4021 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt
4081 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
4141 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
4201 agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat
4261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
4321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
4381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
4441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
4501 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
4561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
4621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
4681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
4741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
4801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
4861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
4921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
4981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
5041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg c
//
pH2B-mTFP1-N1载体图谱质粒图谱pdf版和pH2B-mTFP1-N1载体序列质粒序列等相关资料下载
pH2B-mTFP1-N1质粒载体应用举例
- 上一篇：pMito-dCyOFP2s
- 下一篇：没有了
