pEBFP2-LaminA-C-18

LOCUS       pEBFP2-LaminA-C-18               6717 bp DNA     circular SYN 18-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jul 22, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     589..616
                     /label=PM456-F
     primer_bind     606..633
                     /label=0219-EBFP2-F
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             613..1329
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(1309..1329)
                     /label=0219-EBFP2-R
     CDS             1384..3375
                     /codon_start=1
                     /label=LaminA
                     /translation="ETPSQRRATRSGAQASSTPLSPTRITRLQEKEDLQELNDRLAVYI
                     DRVRSLETENAGLRLRITESEEVVSREVSGIKAAYEAELGDARKTLDSVAKERARLQLE
                     LSKVREEFKELKARNTKKEGDLIAAQARLKDLEALLNSKEAALSTALSEKRTLEGELHD
                     LRGQVAKLEAALGEAKKQLQDEMLRRVDAENRLQTMKEELDFQKNIYSEELRETKRRHE
                     TRLVEIDNGKQREFESRLADALQELRAQHEDQVEQYKKELEKTYSAKLDNARQSAERNS
                     NLVGAAHEELQQSRIRIDSLSAQLSQLQKQLAAKEAKLRDLEDSLARERDTSRRLLAEK
                     EREMAEMRARMQQQLDEYQELLDIKLALDMEIHAYRKLLEGEEERLRLSPSPTSQRSRG
                     RASSHSSQTQGGGSVTKKRKLESTESRSSFSQHARTSGRVAVEEVDEEGKFVRLRNKSN
                     EDQSMGNWQIKRQNGDDPLLTYRFPPKFTLKAGQVVTIWAAGAGATHSPPTDLVWKAQN
                     TWGCGNSLRTALINSTGEEVAMRKLVRSVTVVEDDEDEDGDDLLHHHHGSHCSSSGDPA
                     EYNLRSRTVLCGTCGQPADKASASGSGAQVGGPISSGSSASSVTVTRSYRSVGGSGGGS
                     FGDNLVTRSYLLGNSSPRTQSPQNCSIM"
     primer_bind     1692..1714
                     /label=0312LaminA-F
     primer_bind     2252..2272
                     /label=0312LaminA-F2
     primer_bind     2522..2532
                     /label=0219-EBFP2-F
     primer_bind     complement(2844..2868)
                     /label=0312LaminA-R
     polyA_signal    3505..3626
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3633..4088)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4115..4219
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4221..4578
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4429..4564
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4613..5407
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    5639..5686
                     /label=HSV TK poly(A) signal
                     /note="herpesvirus thymidine kinase polyadenylation signal"
     rep_origin      6015..6603
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgaggggcga gggcgagggc
      721 gatgccacca acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgagc cacggcgtgc agtgcttcgc ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcacc tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcgtcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaacttc aacagccaca acatctatat catggccgtc
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacgtgga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacagcc actacctgag cacccagtcc gtgctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttccgcacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctggcagc ggtggaggca gcgcatccgg cggaagcgga
     1381 agcgagaccc cgtcccagcg gcgcgccacc cgcagcgggg cgcaggccag ctccactccg
     1441 ctgtcgccca cccgcatcac ccggctgcag gagaaggagg acctgcagga gctcaatgat
     1501 cgcttggcgg tctacatcga ccgtgtgcgc tcgctggaaa cggagaacgc agggctgcgc
     1561 cttcgcatca ccgagtctga agaggtggtc agccgcgagg tgtccggcat caaggccgcc
     1621 tacgaggccg agctcgggga tgcccgcaag acccttgact cagtagccaa ggagcgcgcc
     1681 cgcctgcagc tggagctgag caaagtgcgt gaggagttta aggagctgaa agcgcgcaat
     1741 accaagaagg agggtgacct gatagctgct caggctcggc tgaaggacct ggaggctctg
     1801 ctgaactcca aggaggccgc actgagcact gctctcagtg agaagcgcac gctggagggc
     1861 gagctgcatg atctgcgggg ccaggtggcc aagcttgagg cagccctagg tgaggccaag
     1921 aagcaacttc aggatgagat gctgcggcgg gtggatgctg agaacaggct gcagaccatg
     1981 aaggaggaac tggacttcca gaagaacatc tacagtgagg agctgcgtga gaccaagcgc
     2041 cgtcatgaga cccgactggt ggagattgac aatgggaagc agcgtgagtt tgagagccgg
     2101 ctggcggatg cgctgcagga actgcgggcc cagcatgagg accaggtgga gcagtataag
     2161 aaggagctgg agaagactta ttctgccaag ctggacaatg ccaggcagtc tgctgagagg
     2221 aacagcaacc tggtgggggc tgcccacgag gagctgcagc agtcgcgcat ccgcatcgac
     2281 agcctctctg cccagctcag ccagctccag aagcagctgg cagccaagga ggcgaagctt
     2341 cgagacctgg aggactcact ggcccgtgag cgggacacca gccggcggct gctggcggaa
     2401 aaggagcggg agatggccga gatgcgggca aggatgcagc agcagctgga cgagtaccag
     2461 gagcttctgg acatcaagct ggccctggac atggagatcc acgcctaccg caagctcttg
     2521 gagggcgagg aggagaggct acgcctgtcc cccagcccta cctcgcagcg cagccgtggc
     2581 cgtgcttcct ctcactcatc ccagacacag ggtgggggca gcgtcaccaa aaagcgcaaa
     2641 ctggagtcca ctgagagccg cagcagcttc tcacagcacg cacgcactag cgggcgcgtg
     2701 gccgtggagg aggtggatga ggagggcaag tttgtccggc tgcgcaacaa gtccaatgag
     2761 gaccagtcca tgggcaattg gcagatcaag cgccagaatg gagatgatcc cttgctgact
     2821 taccggttcc caccaaagtt caccctgaag gctgggcagg tggtgacgat ctgggctgca
     2881 ggagctgggg ccacccacag cccccctacc gacctggtgt ggaaggcaca gaacacctgg
     2941 ggctgcggga acagcctgcg tacggctctc atcaactcca ctggggaaga agtggccatg
     3001 cgcaagctgg tgcgctcagt gactgtggtt gaggacgacg aggatgagga tggagatgac
     3061 ctgctccatc accaccacgg ctcccactgc agcagctcgg gggaccccgc tgagtacaac
     3121 ctgcgctcgc gcaccgtgct gtgcgggacc tgcgggcagc ctgccgacaa ggcatctgcc
     3181 agcggctcag gagcccaggt gggcggaccc atctcctctg gctcttctgc ctccagtgtc
     3241 acggtcactc gcagctaccg cagtgtgggg ggcagtgggg gtggcagctt cggggacaat
     3301 ctggtcaccc gctcctacct cctgggcaac tccagccccc gaacccagag cccccagaac
     3361 tgcagcatca tgtaaggatc caccggatct agataactga tcataatcag ccataccaca
     3421 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     3481 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     3541 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3601 ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa
     3661 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     3721 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     3781 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     3841 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     3901 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     3961 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     4021 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     4081 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4141 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4201 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     4261 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4321 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4381 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     4441 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4501 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4561 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
     4621 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     4681 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     4741 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     4801 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     4861 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     4921 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     4981 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     5041 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     5101 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     5161 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     5221 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     5281 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     5341 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     5401 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     5461 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     5521 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
     5581 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     5641 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
     5701 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     5761 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     5821 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
     5881 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5941 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6001 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6061 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6121 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     6181 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6241 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6301 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6361 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6421 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6481 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6541 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6601 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6661 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat
//