pmTagBFP2-LaminA-C-18

LOCUS       pmTagBFP2-LaminA-C-18                6699 bp DNA     circular SYN 18-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6699)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jul 22, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     589..615
                     /label=PM456-F
     primer_bind     606..639
                     /label=0219-mTagBFP2-F
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             613..1311
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(1287..1311)
                     /label=0219-mTagBFP2-R
     CDS             1366..3357
                     /codon_start=1
                     /label=LaminA
                     /translation="ETPSQRRATRSGAQASSTPLSPTRITRLQEKEDLQELNDRLAVYI
                     DRVRSLETENAGLRLRITESEEVVSREVSGIKAAYEAELGDARKTLDSVAKERARLQLE
                     LSKVREEFKELKARNTKKEGDLIAAQARLKDLEALLNSKEAALSTALSEKRTLEGELHD
                     LRGQVAKLEAALGEAKKQLQDEMLRRVDAENRLQTMKEELDFQKNIYSEELRETKRRHE
                     TRLVEIDNGKQREFESRLADALQELRAQHEDQVEQYKKELEKTYSAKLDNARQSAERNS
                     NLVGAAHEELQQSRIRIDSLSAQLSQLQKQLAAKEAKLRDLEDSLARERDTSRRLLAEK
                     EREMAEMRARMQQQLDEYQELLDIKLALDMEIHAYRKLLEGEEERLRLSPSPTSQRSRG
                     RASSHSSQTQGGGSVTKKRKLESTESRSSFSQHARTSGRVAVEEVDEEGKFVRLRNKSN
                     EDQSMGNWQIKRQNGDDPLLTYRFPPKFTLKAGQVVTIWAAGAGATHSPPTDLVWKAQN
                     TWGCGNSLRTALINSTGEEVAMRKLVRSVTVVEDDEDEDGDDLLHHHHGSHCSSSGDPA
                     EYNLRSRTVLCGTCGQPADKASASGSGAQVGGPISSGSSASSVTVTRSYRSVGGSGGGS
                     FGDNLVTRSYLLGNSSPRTQSPQNCSIM"
     primer_bind     1674..1696
                     /label=0312LaminA-F
     primer_bind     2234..2254
                     /label=0312LaminA-F2
     primer_bind     complement(2826..2850)
                     /label=0312LaminA-R
     polyA_signal    3487..3608
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3615..4070)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4097..4201
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4203..4560
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4411..4546
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4595..5389
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    5621..5668
                     /label=HSV TK poly(A) signal
                     /note="herpesvirus thymidine kinase polyadenylation signal"
     rep_origin      5997..6585
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatgagcga gctgattaag gagaacatgc acatgaagct gtacatggag
      661 ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
      721 ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
      781 atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc
      841 cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
      901 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
      961 tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa
     1021 acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc
     1081 agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa cgccaagacc
     1141 acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac
     1201 tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg
     1261 gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa ttccggactc
     1321 agatctggca gcggtggagg cagcgcatcc ggcggaagcg gaagcgagac cccgtcccag
     1381 cggcgcgcca cccgcagcgg ggcgcaggcc agctccactc cgctgtcgcc cacccgcatc
     1441 acccggctgc aggagaagga ggacctgcag gagctcaatg atcgcttggc ggtctacatc
     1501 gaccgtgtgc gctcgctgga aacggagaac gcagggctgc gccttcgcat caccgagtct
     1561 gaagaggtgg tcagccgcga ggtgtccggc atcaaggccg cctacgaggc cgagctcggg
     1621 gatgcccgca agacccttga ctcagtagcc aaggagcgcg cccgcctgca gctggagctg
     1681 agcaaagtgc gtgaggagtt taaggagctg aaagcgcgca ataccaagaa ggagggtgac
     1741 ctgatagctg ctcaggctcg gctgaaggac ctggaggctc tgctgaactc caaggaggcc
     1801 gcactgagca ctgctctcag tgagaagcgc acgctggagg gcgagctgca tgatctgcgg
     1861 ggccaggtgg ccaagcttga ggcagcccta ggtgaggcca agaagcaact tcaggatgag
     1921 atgctgcggc gggtggatgc tgagaacagg ctgcagacca tgaaggagga actggacttc
     1981 cagaagaaca tctacagtga ggagctgcgt gagaccaagc gccgtcatga gacccgactg
     2041 gtggagattg acaatgggaa gcagcgtgag tttgagagcc ggctggcgga tgcgctgcag
     2101 gaactgcggg cccagcatga ggaccaggtg gagcagtata agaaggagct ggagaagact
     2161 tattctgcca agctggacaa tgccaggcag tctgctgaga ggaacagcaa cctggtgggg
     2221 gctgcccacg aggagctgca gcagtcgcgc atccgcatcg acagcctctc tgcccagctc
     2281 agccagctcc agaagcagct ggcagccaag gaggcgaagc ttcgagacct ggaggactca
     2341 ctggcccgtg agcgggacac cagccggcgg ctgctggcgg aaaaggagcg ggagatggcc
     2401 gagatgcggg caaggatgca gcagcagctg gacgagtacc aggagcttct ggacatcaag
     2461 ctggccctgg acatggagat ccacgcctac cgcaagctct tggagggcga ggaggagagg
     2521 ctacgcctgt cccccagccc tacctcgcag cgcagccgtg gccgtgcttc ctctcactca
     2581 tcccagacac agggtggggg cagcgtcacc aaaaagcgca aactggagtc cactgagagc
     2641 cgcagcagct tctcacagca cgcacgcact agcgggcgcg tggccgtgga ggaggtggat
     2701 gaggagggca agtttgtccg gctgcgcaac aagtccaatg aggaccagtc catgggcaat
     2761 tggcagatca agcgccagaa tggagatgat cccttgctga cttaccggtt cccaccaaag
     2821 ttcaccctga aggctgggca ggtggtgacg atctgggctg caggagctgg ggccacccac
     2881 agccccccta ccgacctggt gtggaaggca cagaacacct ggggctgcgg gaacagcctg
     2941 cgtacggctc tcatcaactc cactggggaa gaagtggcca tgcgcaagct ggtgcgctca
     3001 gtgactgtgg ttgaggacga cgaggatgag gatggagatg acctgctcca tcaccaccac
     3061 ggctcccact gcagcagctc gggggacccc gctgagtaca acctgcgctc gcgcaccgtg
     3121 ctgtgcggga cctgcgggca gcctgccgac aaggcatctg ccagcggctc aggagcccag
     3181 gtgggcggac ccatctcctc tggctcttct gcctccagtg tcacggtcac tcgcagctac
     3241 cgcagtgtgg ggggcagtgg gggtggcagc ttcggggaca atctggtcac ccgctcctac
     3301 ctcctgggca actccagccc ccgaacccag agcccccaga actgcagcat catgtaagga
     3361 tccaccggat ctagataact gatcataatc agccatacca catttgtaga ggttttactt
     3421 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     3481 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3541 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3601 gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     3661 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     3721 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     3781 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     3841 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     3901 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     3961 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     4021 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
     4081 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4141 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4201 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     4261 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     4321 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4381 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4441 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     4501 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     4561 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     4621 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     4681 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     4741 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     4801 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     4861 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     4921 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     4981 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     5041 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     5101 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
     5161 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     5221 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     5281 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     5341 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     5401 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     5461 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     5521 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa
     5581 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     5641 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     5701 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     5761 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     5821 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt
     5881 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5941 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6001 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6061 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6121 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     6181 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6241 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6301 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6361 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6421 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6481 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6541 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6601 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     6661 tcccctgatt ctgtggataa ccgtattacc gccatgcat
//