| 质粒名称: | pLenti CMV/TO eGFP Puro (w159-1) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 17481 |
| 存储实验室: | Eric Campeau |
|
载体骨架基本信息 |
|
| 载体名称: | p156RRL-sinPPT-CMV-GFP-PRE/Nhe I |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 8052 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | enhanced green fluorescent protein |
| 插入大小: | 719 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | |
|
文献引用方法 |
|
| 材料和方法部分: | pLenti CMV/TO eGFP Puro (w159-1) was a gift from Eric Campeau (Addgene plasmid # 17481) |
| 参考文献部分: | A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pLenti CMV/TO eGFP Puro (w159-1) 8771 bp DNA SYN
DEFINITION pLenti CMV/TO eGFP Puro (w159-1)
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..8771
/organism="pLenti CMV/TO eGFP Puro (w159-1)"
/mol_type="other DNA"
promoter 62..90
/label="AmpR_promoter"
gene 132..992
/label="Ampicillin"
/gene="Ampicillin"
CDS 132..992
/label="ORF frame 3"
rep_origin 1147..1766
/label="pBR322_origin"
promoter 2075..2104
/label="lac_promoter"
misc_feature 2118..2140
/label="M13_pUC_rev_primer"
promoter 2139..2157
/label="M13_reverse_primer"
promoter 2174..2193
/label="T3_promoter"
misc_feature 2448..2628
/label="HIV-1_5_LTR"
misc_feature 2448..2628
/label="truncHIV-1_3_LTR"
misc_feature 2736..2780
/label="HIV-1_psi_pack"
CDS 3168..3668
/label="ORF frame 3"
misc_feature 3290..3523
/label="RRE"
CDS 3629..4171
/label="ORF frame 2"
misc_feature 4016..4031
/label="cPPT"
promoter 4149..4653
/label="CMV_immearly_promoter"
misc_feature 4156..4443
/label="CAG_enhancer"
misc_feature 4610..4630
/label="CMV_fwd_primer"
promoter 4611..4680
/label="CMV_promoter"
misc_feature 4654..4673
/label="pCEP_fwd_primer"
misc_feature 4656..4680
/label="LNCX_primer"
misc_feature 4764..4788
/label="attB1"
CDS 4794..5573
/label="ORF frame 3"
gene 4857..5570
/label="EGFP"
/gene="EGFP"
misc_feature complement(4899..4920)
/label="EGFP_N_primer"
misc_feature 5037..5066
/label="Y66 (EGFP)"
misc_feature 5507..5528
/label="EGFP_C_primer"
misc_feature 5598..5622
/label="attB2"
misc_feature 5655..6230
/label="WPRE"
CDS 5743..6372
/label="ORF frame 1"
misc_feature complement(6290..6313)
/label="MSCV_rev_primer"
CDS 6535..7374
/label="ORF frame 1"
misc_feature 6687..6706
/label="mPGK_F_primer"
CDS complement(6700..7425)
/label="ORF frame 3"
gene 6775..7374
/label="puro (variant)"
/gene="puro (variant)"
misc_feature 7495..7510
/label="cPPT"
misc_feature 7495..7516
/label="U3PPT"
misc_feature 7565..7745
/label="HIV-1_5_LTR"
misc_feature 7565..7745
/label="truncHIV-1_3_LTR"
terminator 7820..7939
/label="SV40_PA_terminator"
misc_feature 7908..7927
/label="EBV_rev_primer"
rep_origin 7957..8034
/label="SV40_origin"
misc_feature 8019..8038
/label="SV40pro_F_primer"
promoter complement(8125..8143)
/label="T7_promoter"
promoter complement(8153..8169)
/label="M13_forward20_primer"
misc_feature 8162..8305
/label="lacZ_a"
misc_feature complement(8162..8184)
/label="M13_pUC_fwd_primer"
rep_origin 8331..8637
/label="f1_origin"
ORIGIN
1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag
4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgataagct
4141 tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
4201 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
4261 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
4321 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
4381 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
4441 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
4501 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
4561 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
4621 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
4681 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccgact CTAGTCCAGT
4741 GTGGTGGAAT TCTGCAGATA TCAACAAGTT TGTACAAAAA AGCAGGCTCC ACCATGGGAA
4801 CCAATTCAGT CGACTGGTAC CGCGGGCCCG GGATCCACCG GCCGGTCGCC ACCATGGTGA
4861 GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG
4921 TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC
4981 TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA
5041 CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG
5101 ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG
5161 ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC
5221 GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG
5281 AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA
5341 AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT
5401 ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA
5461 GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG
5521 AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG TAAAGCGGCC
5581 GCACTCGAGA TATCTAGACC CAGCTTTCTT GTACAAAGTG GTTGATATCC AGCACAGTGG
5641 CGGCCGCTCG Acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
5701 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
5761 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
5821 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
5881 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
5941 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
6001 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
6061 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
6121 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
6181 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
6241 cctgGAATTC TACCGGGTAG GGGAGGCGCT TTTCCCAAGG CAGTCTGGAG CATGCGCTTT
6301 AGCAGCCCCG CTGGGCACTT GGCGCTACAC AAGTGGCCTC TGGCCTCGCA CACATTCCAC
6361 ATCCACCGGT AGGCGCCAAC CGGCTCCGTT CTTTGGTGGC CCCTTCGCGC CACCTTCTAC
6421 TCCTCCCCTA GTCAGGAAGT TCCCCCCCGC CCCGCAGCTC GCGTCGTGCA GGACGTGACA
6481 AATGGAAGTA GCACGTCTCA CTAGTCTCGT GCAGATGGAC AGCACCGCTG AGCAATGGAA
6541 GCGGGTAGGC CTTTGGGGCA GCGGCCAATA GCAGCTTTGC TCCTTCGCTT TCTGGGCTCA
6601 GAGGCTGGGA AGGGGTGGGT CCGGGGGCGG GCTCAGGGGC GGGCTCAGGG GCGGGGCGGG
6661 CGCCCGAAGG TCCTCCGGAG GCCCGGCATT CTGCACGCTT CAAAAGCGCA CGTCTGCCGC
6721 GCTGTTCTCC TCTTCCTCAT CTCCGGGCCT TTCGACCTGC AGCCCAAGCT TACCATGACC
6781 GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC
6841 CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGATCC GGACCGCCAC
6901 ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC
6961 AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC
7021 GAAGCGGGGG CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG
7081 CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG
7141 TGGTTCCTGG CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC
7201 GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC
7261 TCCGCGCCCC GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC
7321 GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGACGCCCG
7381 CCCCACGACC CGCAGCGCCC GACCGAAAGG AGCGCACGAC CCCATGCATC TCGAGGGCCC
7441 GGTACcttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
7501 aaagggggga ctggaagggc tagctcactc ccaacgaaga caagatctgc tttttgcttg
7561 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct gcctagggaa
7621 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
7681 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
7741 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
7801 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
7861 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
7921 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt
7981 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
8041 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
8101 tgcgtcgaga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
8161 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
8221 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
8281 aacagttgcg cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg
8341 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
8401 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
8461 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
8521 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
8581 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
8641 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
8701 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
8761 ttacaatttc c
//
