pBluescriptR-slc26a2

LOCUS       pBluescriptR-slc26a2                9178 bp DNA     circular SYN 22-FEB-2024
DEFINITION  TSN20240201-0571-00002_373_20240201-NJAG01-B01_A09_i47.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 7, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(78..533)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     675..691
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        698..716
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(749..782)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             970..3189
                     /product="Homo sapiens solute carrier family 26 (sulfate 
                     transporter), member 2, mRNA (cDNA clone MGC:71706 
                     IMAGE:30341764), complete cdsSequence ID: BC059390.1"
                     /label=SLC26A2 123-2342
                     /note="Homo sapiens solute carrier family 26 (sulfate 
                     transporter), member 2, mRNA (cDNA clone MGC:71706 
                     IMAGE:30341764), complete cdsSequence ID: BC059390.1"
     CDS             3190..4482
                     /product="Homo sapiens solute carrier family 26 (sulfate 
                     transporter), member 2, mRNA (cDNA clone MGC:71706 
                     IMAGE:30341764), complete cdsSequence ID: BC059390.1"
                     /label=slc26a2 2343-3566
     promoter        complement(4483..4501)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4522..4538)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4546..4562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4570..4600)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4615..4636
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    4742..5682
                     /label=ColE1
                     /note="ColE1"
     rep_origin      complement(4924..5512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5683..6543)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6544..6648)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7122..7138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        7165..7468
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7469..7672
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          7810..7906
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             7967..8818
                     /product="Mus musculus ribosomal protein L7 (Rpl7), mRNA
                     Sequence ID: NM_011291.5"
                     /label=Rpl7 - Mouse
     CDS             7970..7996
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    8874..9008
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9119..9135)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9143..9159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
       61 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
      121 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
      181 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
      241 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
      301 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
      361 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
      421 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
      481 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
      541 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      601 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      661 ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga
      721 attggagctc caccgcggtg gcggccgcat aacttcgtat agcatacatt atacgaagtt
      781 atggatcagg ccaaatcggc cgagctcgaa ttcgtcgagc tctagcgagg tgacagcgta
      841 gaaccagata gctctgtcgg cgccgcggtg tccacctcag tcaggccacg gtggaagacg
      901 cgtgccgcgg cgcctggttg cctgcagcgg cccggacccg agaggaagct gaaccatcta
      961 tctccagaaa tgtcttcaga aagtaaagag caacataacg tttcacccag agactcagct
     1021 gaaggaaatg acagttatcc atctgggatc catctggaac ttcaaaggga atcaagtact
     1081 gacttcaagc aatttgagac caatgatcaa tgcagacctt atcataggat ccttattgag
     1141 cgtcaagaga aatcagatac aaacttcaag gagtttgtta ttaaaaagct gcagaagaat
     1201 tgccagtgca gtccagccaa agccaaaaat atgattttag gtttccttcc tgttttgcag
     1261 tggctcccaa aatacgacct aaagaaaaac attttagggg atgtgatgtc aggcttgatt
     1321 gtgggcatat tattggtgcc ccagtccatt gcttattccc tgctggctgg ccaagaacct
     1381 gtctatggtc tgtacacatc tttttttgcc agcatcattt attttctctt gggtacctcc
     1441 cgtcacatct ctgtgggcat ttttggagta ctgtgcctta tgattggtga gacagttgac
     1501 cgagaactac agaaagctgg ctatgacaat gcccatagtg ctccttcctt aggaatggtt
     1561 tcaaatggga gcacattatt aaatcataca tcagacagga tatgtgacaa aagttgctat
     1621 gcaattatgg ttggcagcac tgtaaccttt atagctggag tttatcaggt agcgatgggc
     1681 ttctttcaag tgggttttgt ttctgtctac ctctcagatg ccttgctgag tggatttgtc
     1741 actggtgcct ccttcactat tcttacatct caggccaagt atcttcttgg gctcaacctt
     1801 cctcggacta atggtgtggg ctcactcatc actacctgga tacatgtctt cagaaacatc
     1861 cataagacca atctctgtga tcttatcacc agccttttgt gccttttggt tcttttgcca
     1921 accaaagaac tcaatgaaca cttcaaatcc aagcttaagg caccgattcc tattgaactt
     1981 gttgttgttg tagcagccac attagcctct cattttggaa aactacatga aaattataat
     2041 tctagtattg ctggacatat tcccactggg tttatgccac ccaaagtacc agaatggaac
     2101 ctaattccta gtgtggctgt agatgcaata gctatttcca tcattggttt tgctatcact
     2161 gtatcacttt ctgagatgtt tgccaagaaa catggttaca cagtcaaagc aaaccaggaa
     2221 atgtatgcca ttggcttttg taatatcatc ccttccttct tccactgttt tactactagt
     2281 gcagctcttg caaagacatt ggttaaagaa tcaacaggct gccatactca gctttctggt
     2341 gtggtaacag ccctggttct tttgttggtc ctcctagtaa tagctccttt gttctattcc
     2401 cttcaaaaaa gtgtccttgg tgtgatcaca attgtaaatc tacggggagc ccttcgtaaa
     2461 tttagggatc ttcccaaaat gtggagtatt agtagaatgg atacagttat ctggtttgtt
     2521 actatgctgt cctctgcact gctaagtact gaaataggcc tacttgttgg ggtttgtttt
     2581 tctatatttt gtgtcatcct ccgcactcag aagccaaaga gttcactgct tggcttggtg
     2641 gaagagtctg aggtctttga atctgtgtct gcttacaaga accttcagac taagccaggc
     2701 atcaagattt tccgctttgt agcccctctc tactacataa acaaagaatg ctttaaatct
     2761 gctttataca aacaaactgt caacccaatc ttaataaagg tggcttggaa gaaggcagca
     2821 aagagaaaga tcaaagaaaa agtagtgact cttggtggaa tccaggatga aatgtcagtg
     2881 caactttccc atgatccctt ggagctgcat actatagtga ttgactgcag tgcaattcaa
     2941 tttttagata cagcagggat ccacacactg aaagaagttc gcagagatta tgaagccatt
     3001 ggaatccagg ttctgctggc tcagtgcaat cccactgtga gggattccct aaccaacgga
     3061 gaatattgca aaaaggaaga agaaaacctt ctcttctata gtgtgtatga agcgatggct
     3121 tttgcagaag tatctaaaaa tcagaaagga gtatgtgttc ccaatggtct gagtcttagt
     3181 agtgattaat tgagaaggta gatagaagaa tgtctagcca ataggttgaa atttcaagtg
     3241 tccaacattt cccagttcca cagtgggaaa ttttgcacac ttgaaatttt aaccaagtgg
     3301 ctagatatta ttcctccttt gaagctaatg gcatttgtat atacacactg cagcagagct
     3361 tgtagctgga cagagtcaaa aagaagaaaa tacggtttca ggctttcttg cagatatgaa
     3421 gtattcttgg aatgcaataa gtatgtattg aactgtactg taaagtagct ccaaaactta
     3481 attactctcc tgttttaggg gttatacatt tggactgtgc attctccaag agatgaagcg
     3541 gtgaagttgg gatttacatt ggaagtgctg tagacttctt tatgtggctc agtggagaga
     3601 gggaaagaat gttgcacctg ctctagtacc ataggtcaag aggcttctgg atcacaaagt
     3661 cataactaga caggtttgtt cttgtagttt tctatcccca gtctttgctc cccagatggc
     3721 agtagttttt agtaggaaag tgccattcct gtccttaagg cacagtctca tcagaagtct
     3781 aatacctggg caggtttata acatcctgag agccagcctg acattagaca gaataccctt
     3841 tgtaatacat tggaaatttt tactcatgcc tttttgttta ggataaatag gtaagcacaa
     3901 agagctcttc aaaatcagaa aaaacaatag gagtccttcc ttgtcttttc tgtgatctct
     3961 gtccttgttt ctgagacttt ctctaccatt aagctctatt ttagctttca gttattctag
     4021 tttgtttccc atggaatctg tcctaaactg gtgtttttgt cagtgacagt cttgccagtc
     4081 agcaatttct aacagcattt taaatgagtt tgatgtacag taaatattga tgacaatgac
     4141 agcttttaac tcttcaagtc acctaaagct attatgcagg aggatttaga agtcacattc
     4201 ataaaaccca agtgctatgg gtgtattatt catgatagct ggcccacagg tcatgaattg
     4261 aggaggaatt tgctttcaaa aagcaagaat gtccaacact gaaagtttat agttttatat
     4321 ttggaccttg aaaggtaaga aaaaaccagg ttctccaaag ttaggaatag ggaactaatt
     4381 tatgaaacag ccatcttaaa aaaaaaaaaa aaactctcca ccgttggatc cggccataag
     4441 ggcctgatcc ttcgaggggg ggcccggtac cagcttttgt tccctttagt gagggttaat
     4501 ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     4561 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     4621 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     4681 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4741 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4801 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4861 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4921 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4981 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     5041 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     5101 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5161 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     5221 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     5281 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5341 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     5401 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5461 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5521 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5581 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     5641 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     5701 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5761 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5821 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5881 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5941 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     6001 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     6061 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     6121 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     6181 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     6241 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     6301 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     6361 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     6421 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     6481 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     6541 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     6601 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     6661 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     6721 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     6781 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     6841 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     6901 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     6961 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     7021 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     7081 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     7141 gttcgagctt gcatgcctgc aggtcgttac ataacttacg gtaaatggcc cgcctggctg
     7201 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     7261 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     7321 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     7381 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     7441 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     7501 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     7561 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     7621 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
     7681 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
     7741 ggaccgatcc agcctccgga ctctagagga tccggtacta gaggaactga aaaaccagaa
     7801 agttaactgg taagtttagt ctttttgtct tttatttcag gtcccggatc cggtggtggt
     7861 gcaaatcaaa gaactgctcc tcagtggatg ttgcctttac ttctaggcct gtacggaagt
     7921 gttacttctg ctctaaaagc tgcggaattg tacccgcggg cccaccatgt acccatacga
     7981 tgttccagat tacgctctta tggccatgga ggctgttcca gagaagaaaa agaaggttgc
     8041 cactgtgcca ggaaccctta agaaaaaggt tcctgctggg ccaaaaactc tcaagaaaaa
     8101 ggttcccgct gtgccagaaa ccctcaagaa aaagcgaagg aatttcgcag agttgaaggt
     8161 gaagcgcctg aggaagaagt ttgccctgaa gacacttcga aaggcaagga ggaagctcat
     8221 ctatgagaag gcaaagcact atcacaagga gtacaggcag atgtaccgca ctgagattcg
     8281 gatggccagg atggcaagga aagctggcaa cttctatgtg cccgcagaac caaagctggc
     8341 ctttgtcatc agaattcgag gtatcaatgg agtaagccca aaggttcgta aggttctgca
     8401 gctccttcgt cttcgacaga tcttcaatgg cacctttgtt aagctcaaca aggcttcaat
     8461 taacatgctg cggattgtgg agccatacat tgcatggggg taccccaacc tgaagtcagt
     8521 aaacgagctc atctacaagc gaggctacgg caaaatcaac aagaagcgga ttgccttgac
     8581 agataattcc ttgattgctc ggtctcttgg taagtttggc atcatctgca tggaggatct
     8641 aattcatgag atctatacag tcgggaaacg cttcaaggaa gcaaataact tcctgtggcc
     8701 cttcaagtta tcttccccac gaggtgggat gaagaaaaag acaactcact ttgtagaagg
     8761 tggagatgct ggcaacaggg aagaccagat aaacaggctt attagacgga tgaactaacc
     8821 atggaggccc gaattcggtc gaccgagatc tctcgaggta ccgcggccgc ggggatccag
     8881 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     8941 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     9001 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     9061 aggttttttc ggatcctcta gagtcgatct gcaggcatgc tagcttggcg taatcatggt
     9121 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagc
//