| 质粒名称: | Tet-pLKO-puro-Scrambled |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 47541 |
| 存储实验室: | Charles Rudin |
|
载体骨架基本信息 |
|
| 载体名称: | Tet-pLKO-puro |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8775 bp |
| 完整质粒大小: | 10633 bp |
| 载体修饰: | 人细胞色素c氧化酶亚基VIII（human cytochrome C oxidase subunit VIII） |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Scrambled shRNA |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | Tet-pLKO-puro-Scrambled was a gift from Charles Rudin (Addgene plasmid # 47541 ; http://n2t.net/addgene:47541 ; RRID:Addgene_47541) |
| 参考文献部分: | Comprehensive genomic analysis identifies SOX2 as a frequently amplified gene in small-cell lung cancer. Rudin CM, Durinck S, Stawiski EW, Poirier JT, Modrusan Z, Shames DS, Bergbower EA, Guan Y, Shin J, Guillory J, Rivers CS, Foo CK, Bhatt D, Stinson J, Gnad F, Haverty PM, Gentleman R, Chaudhuri S, Janakiraman V, Jaiswal BS, Parikh C, Yuan W, Zhang Z, Koeppen H, Wu TD, Stern HM, Yauch RL, Huffman KE, Paskulin DD, Illei PB, Varella-Garcia M, Gazdar AF, de Sauvage FJ, Bourgon R, Minna JD, Brock MV, Seshagiri S. Nat Genet. 2012 Oct;44(10):1111-6. doi: 10.1038/ng.2405. Epub 2012 Sep 2. 10.1038/ng.2405 PubMed 22941189 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Tet-pLKO-puro-Scrambled 8815 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Tet inducible scrambled shRNA..
ACCESSION .
VERSION .
KEYWORDS Tet-pLKO-puro-Scrambled
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8815)
AUTHORS Rudin CM, Durinck S, Stawiski EW, Poirier JT, Modrusan Z, Shames DS,
Bergbower EA, Guan Y, Shin J, Guillory J, Rivers CS, Foo CK, Bhatt
D, Stinson J, Gnad F, Haverty PM, Gentleman R, Chaudhuri S,
Janakiraman V, Jaiswal BS, Parikh C, Yuan W, Zhang Z, Koeppen H, Wu
TD, Stern HM, Yauch RL, Huffman KE, Paskulin DD, Illei PB,
Varella-Garcia M, Gazdar AF, de Sauvage FJ, Bourgon R, Minna JD,
Brock MV, Seshagiri S
TITLE Comprehensive genomic analysis identifies SOX2 as a frequently
amplified gene in small-cell lung cancer.
JOURNAL Nat Genet. 2012 Oct;44(10):1111-6. doi: 10.1038/ng.2405. Epub 2012
Sep 2.
PUBMED 22941189
REFERENCE 2 (bases 1 to 8815)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8815
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 258..278
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 489..722
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 794..915
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(831..850)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 885..904
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 955..1090
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 1017..1036
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter complement(1111..1129)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1139..1156)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(1139..1155)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1148..1170)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 1297..1752
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1384..1403)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 1594..1615
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 1778..1882
/gene="bla"
/label=AmpR promoter
CDS 1883..2743
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(2101..2120)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 2914..3502
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3403..3422
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3656..3673
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 3790..3811
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3826..3856
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3864..3880
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 3869..3891
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 3888..3904
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 3888..3904
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 3923..3943
/label=T3
/note="T3 promoter, forward primer"
promoter 3925..3943
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 3971..4197
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 4198..4378
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 4425..4550
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 5043..5276
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
primer_bind 5805..5824
/label=H1
/note="Human H1 promoter, forward primer"
protein_bind 5842..5860
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 5870..5888
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
misc_feature 5988..6105
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 6154..6664
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 6583..6601
/label=hPGK-F
/note="Human PGK promoter, forward primer"
intron 6673..7245
/label=-beta-globin intron
/note="intron from rabbit beta-globin gene"
primer_bind 7253..7272
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
promoter 7300..7318
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 7329..7946
/codon_start=1
/gene="tetR from transposon Tn10"
/product="tetracycline repressor TetR"
/label=TetR
/note="TetR binds to the tetracycline operator tetO to
inhibit transcription. This inhibition can be relieved by
adding tetracycline or doxycycline."
/translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESG"
primer_bind complement(7402..7421)
/label=Tet-R
/note="Tetracycline resistance gene, reverse primer"
misc_feature 7984..8557
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(8151..8168)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 8378..8397
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 8577..361
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(8577..8596)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
ORIGIN
1 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
61 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
121 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
181 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
241 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
301 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
361 acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcggt
421 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
481 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
541 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
601 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
661 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
721 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
781 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
841 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
901 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
961 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
1021 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
1081 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
1141 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
1201 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
1261 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
1321 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
1381 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
1441 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
1501 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
1561 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
1621 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
1681 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
1741 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
1801 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
1861 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
1921 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
1981 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
2041 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
2101 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
2161 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
2221 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
2281 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
2341 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
2401 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
2461 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
2521 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
2581 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
2641 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
2701 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
2761 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
2821 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
2881 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
2941 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
3001 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
3061 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
3121 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
3181 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
3241 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
3301 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
3361 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
3421 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
3481 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
3541 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
3601 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
3661 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
3721 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
3781 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
3841 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
3901 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
3961 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
4021 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
4081 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
4141 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
4201 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
4261 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
4321 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
4381 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
4441 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
4501 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
4561 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
4621 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
4681 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
4741 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
4801 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
4861 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
4921 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
4981 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
5041 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
5101 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
5161 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
5221 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
5281 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
5341 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
5401 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
5461 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
5521 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
5581 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
5641 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
5701 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
5761 tatcgatcac gagactagcc tcgagcggcc gcaatatttg catgtcgcta tgtgttctgg
5821 gaaatcacca taaacgtgaa atccctatca gtgatagaga cttataagtt ccctatcagt
5881 gatagagaca ccggcctaag gttaagtcgc cctcgctcga gcgagggcga cttaacctta
5941 ggtttttaat tctcgacctc gagacaaatg gcagtattca tccacaattt taaaagaaaa
6001 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
6061 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
6121 gacagcagag atccactttg gccgcggctc gagggggttg gggttgcgcc ttttccaagg
6181 cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg
6241 cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc
6301 cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc
6361 cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct
6421 cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa
6481 tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc
6541 ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa
6601 gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc
6661 ccagggggat ctgtgagttt ggggaccctt gattgttctt tctttttcgc tattgtaaaa
6721 ttcatgttat atggaggggg caaagttttc agggtgttgt ttagaatggg aagatgtccc
6781 ttgtatcacc atggaccctc atgataattt tgtttctttc actttctact ctgttgacaa
6841 ccattgtctc ctcttatttt cttttcattt tctgtaactt tttcgttaaa ctttagcttg
6901 catttgtaac gaatttttaa attcactttt gtttatttgt cagattgtaa gtactttctc
6961 taatcacttt tttttcaagg caatcagggt atattatatt gtacttcagc acagttttag
7021 agaacaattg ttataattaa atgataaggt agaatatttc tgcatataaa ttctggctgg
7081 cgtggaaata ttcttattgg tagaaacaac tacatcctgg tcatcatcct gcctttctct
7141 ttatggttac aatgatatac actgtttgag atgaggataa aatactctga gtccaaaccg
7201 ggcccctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg
7261 ctggttattg tgctgtctca tcattttggc aaagaattgt aatacgactc actatagggc
7321 gaattgatat gtctagatta gataaaagta aagtgattaa cagcgcatta gagctgctta
7381 atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc
7441 agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc ttagccattg
7501 agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc tggcaagatt
7561 ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc gatggagcaa
7621 aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa aatcaattag
7681 cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc agcgctgtgg
7741 ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct aaagaagaaa
7801 gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc gaattatttg
7861 atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata tgcggattag
7921 aaaaacaact taaatgtgaa agtgggtccg cgtacagcgg ctcccgggag ttctagggat
7981 ctgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
8041 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
8101 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
8161 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
8221 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
8281 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
8341 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
8401 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
8461 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
8521 cggggacgtg gttttccttt gaaaaacacg atgataagga tccaccggag cttaccatga
8581 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
8641 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
8701 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
8761 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccgga
//
