LOCUS pDisplay-GRAB_NTS1.0 8657 bp DNA circular SYN 23-JAN-2024
DEFINITION Expresses the genetically-encoded fluorescent neurotensin (NTS)
sensor GRAB_NTS1.0 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pDisplay-GRAB_NTS1.0
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8657)
TITLE A toolkit of highly selective and sensitive genetically encoded
neuropeptide sensors
REFERENCE 2 (bases 1 to 8657)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8657
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 10..389
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 390..593
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 544..564
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 638..657
/label=T7
/note="T7 promoter, forward primer"
promoter 638..656
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 722..2848
/codon_start=1
/label=NTS1.0
/translation="METDTLLLWVLLLWVPGSTGDTSLYKKVGTTGRLNSSAPGTPGTP
AADPFQRAQAGLEEALLAPGFGNASGNASERVLAAPSSELDVNTDIYSKVLVTAVYLAL
FVVGTVGNTVTAFTLARKKSLQSLQSTVHYHLGSLALSDLLTLLLAMPVELYNFIWVHH
PWAFGDAGCRGYYFLRDACTYATALNVASLSVERYLAICHPFKAKTLMSRSRTKKFISA
IWLASALLAVPMLFTMGEQNRSADGQHAGGLVCTPTIHTATVKVVIQVNTFMSFIFPMV
VISVLNTIIANKLTVMVRQAAEQLNGAPGEPAPAGPRDTDALDLEEGGNVYIKADKQKN
GIKAAFNIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHM
VLLEFVTAAGITWGMDELYKGGTGGSMVRKGEELFTGVVPILVELDGDVNGHKFSVGGE
GEGDATEGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEG
YIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNTGAAAR
WRGREPNRVQALRHGVRVLRAVVIAFVVCWLPYHVRRLMFCYISDEQWTPFLYDFYHYF
YMVTNALFYVSSTINPILYNLVSANFRHIFLATLACLCPVWRRRRKRPAFSRKADSVSS
NHTLSSNATRETLY"
sig_peptide 722..784
/codon_start=1
/product="leader sequence from mouse immunoglobulin kappa
light chain"
/label=Ig-kappa leader
/translation="METDTLLLWVLLLWVPGSTGD"
protein_bind 785..809
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
primer_bind 1915..1936
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(2042..2063)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2303..2322)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
misc_feature 2856..3441
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(3023..3040)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 3249..3268
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 3442..4149
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(3587..3605)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 3855..3874
/label=mCherry-F
/note="mCherry, forward primer"
CDS 4207..4236
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 4240..4386
/codon_start=1
/product="transmembrane domain from platelet derived growth
factor receptor beta"
/label=PDGFR-beta TM domain
/translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
KKPR"
primer_bind complement(4408..4425)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4414..4638
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin complement(4770..5358)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4850..4869)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
polyA_signal 5687..5734
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
primer_bind 5759..5778
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
CDS complement(5966..6760)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTYGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6077..6096)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 6687..6706
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind complement(6859..6878)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
enhancer 6957..7148
/label=SV40 enhancer
/note="enhancer for the SV40 early promoter (Herr, 1993)"
primer_bind 7105..7125
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
CDS complement(7208..8068)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7831..7850
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8069..8173)
/gene="bla"
/label=AmpR promoter
rep_origin 8200..8655
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8287..8306)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8497..8518
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
121 gaccgcccaa cgacccctgc ccattgacgt caataatgac gtatgttccc atagtaacgc
181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcac
721 catggagaca gacacactcc tgctatgggt actgctgctc tgggttccag gttccactgg
781 tgacacaagt ttgtacaaaa aagttggcac caccggtcgc ctcaacagct ccgcgccggg
841 aaccccgggc acgccggccg ccgacccctt ccagcgggcg caggccggac tggaggaggc
901 gctgctggcc ccgggcttcg gcaacgcttc gggcaacgcg tcggagcgcg tcctggcggc
961 acccagcagc gagctggacg tgaacaccga catctactcc aaggtgctgg tgaccgccgt
1021 gtacctggcg ctcttcgtgg tgggcacggt gggcaacacg gtgacggcgt tcacgctggc
1081 gcggaagaag tcgctgcaga gcctgcagag cacggtgcat taccacctgg gcagcctggc
1141 gctgtccgac ctgctcaccc tgctgctggc catgcccgtg gagctgtaca acttcatctg
1201 ggtgcaccac ccctgggcct tcggcgacgc cggctgccgc ggctactact tcctgcgcga
1261 cgcctgcacc tacgccacgg ccctcaacgt ggccagcctg agtgtggagc gctacctggc
1321 catctgccac cccttcaagg ccaagaccct catgtcccga agccgcacca agaagttcat
1381 cagcgccatc tggctcgcct cggccctgct ggcggtgcct atgctgttca ccatgggcga
1441 gcagaaccgc agcgccgacg gccagcacgc cggcggcctg gtgtgcaccc ccaccatcca
1501 cactgccacc gtcaaggtcg tcatacaggt caacaccttc atgtccttca tattccccat
1561 ggtggtcatc tcggtcctga acaccatcat cgccaacaag ctgaccgtca tggtacgcca
1621 ggcggccgag cagctcaacg gcgcccctgg cgagcccgcg ccggccgggc cgcgcgacac
1681 cgacgcgctg gacctggagg agggaggaaa cgtctatatc aaggccgaca agcagaagaa
1741 cggcatcaag gcggcgttca atatccgcca caacatcgag gacggcggcg tgcagctcgc
1801 ctaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
1861 ctacctgagc gtgcagtcca aactttcgaa agaccccaac gagaagcgcg atcacatggt
1921 cctgctggag ttcgtgaccg ccgccgggat cacttggggc atggacgagc tgtacaaggg
1981 cggtaccgga gggagcatgg tgagaaaggg cgaggagctg ttcaccgggg tggtgcccat
2041 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgggcg gcgagggtga
2101 gggcgatgcc accgagggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
2161 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
2221 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacatcca
2281 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
2341 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
2401 caacatcctg gggcacaagc tggagtacaa caccggagca gcagcacgct ggcgcgggcg
2461 ggagcctaat agggtccagg ccctgcggca cggcgtgcgc gtcctacgtg cagtggtcat
2521 cgcctttgtg gtctgctggc tgccctacca cgtgcggcgc ctcatgttct gctacatctc
2581 ggatgagcag tggactccgt tcctctatga cttctaccac tacttctaca tggtgaccaa
2641 cgcactcttc tacgtcagct ccaccatcaa ccccatcctg tacaacctcg tctctgccaa
2701 cttccgccac atcttcctgg ccacactggc ctgcctctgc ccggtgtggc ggcgcaggag
2761 gaagaggcca gccttctcga ggaaggccga cagcgtgtcc agcaaccaca ccctctccag
2821 caatgccacc cgcgagacgc tgtactaggc tagcgcccct ctccctcccc cccccctaac
2881 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
2941 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
3001 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
3061 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
3121 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaacgcca cgtgtataag
3181 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
3241 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
3301 cccattgtat gggatctgat ctggggcctc ggtacacatg ctttacatgt gtttagtcga
3361 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
3421 gatgataata tggccacaac catggtgagc aagggcgagg aggataacat ggccatcatc
3481 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
3541 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
3601 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
3661 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
3721 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
3781 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
3841 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
3901 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
3961 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
4021 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
4081 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
4141 ctgtacaagg atggtaaaaa gaagaaaaag aagtcaaaga caaagtgtgt aattatgtaa
4201 gtcgacgaac aaaaactcat ctcagaagag gatctgaatg ctgtgggcca ggacacgcag
4261 gaggtcatcg tggtgccaca ctccttgccc tttaaggtgg tggtgatctc agccatcctg
4321 gccctggtgg tgctcaccat catctccctt atcatcctca tcatgctttg gcagaagaag
4381 ccacgttagg cggccgctcg agatcagcct cgactgtgcc ttctagttgc cagccatctg
4441 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
4501 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
4561 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
4621 atgcggtggg ctctatggct tctgaggcgg aaagaaccag tggcggtaat acggttatcc
4681 acagaatcgg gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
4741 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
4801 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
4861 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
4921 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
4981 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
5041 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacgc
5101 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
5161 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
5221 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
5281 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
5341 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
5401 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
5461 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaacctgag
5521 gctatggcag ggcctgccgc cccgacgttg gctgcgagcc ctgggccttc acccgaactt
5581 ggggggtggg gtggggaaaa ggaagaaacg cgggcgtatt ggccccaatg gggtctcggt
5641 ggggtatcga cagagtgcca gccctgggac cgaaccccgc gtttatgaac aaacgaccca
5701 acaccgtgcg ttttattctg tctttttatt gccgtcatag cgcgggttcc ttccggtatt
5761 gtctccttcc gtgtttcagt tagcctcccc ctagggtggg cgaagaactc cagcatgaga
5821 tccccgcgct ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt
5881 tcatagaagg cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg
5941 gtcatttcga accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga
6001 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc
6061 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca
6121 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg
6181 gcaagcaggc atcgccatag gtcacgacga gatcctcgcc gtcgggcatg ctcgccttga
6241 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat
6301 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt
6361 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
6421 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca
6481 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc
6541 ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg cagttcattc agggcaccgg
6601 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg
6661 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
6721 cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg
6781 tctcttgatc gatctttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg
6841 aatagctcag aggccgggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
6901 gcggagaatg ggcggaactg ggcggagtta ggggcggaat gggcggagtt aggggcggga
6961 ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg
7021 gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
7081 ctggggagcc tggggacttt ccacacccta actgacacac attccacagc tggttctttc
7141 cgcctcagga ctcttccttt ttcaataaat caatctaaag tatatatgag taaacttggt
7201 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
7261 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
7321 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
7381 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
7441 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
7501 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
7561 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
7621 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
7681 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
7741 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
7801 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
7861 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
7921 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
7981 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
8041 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
8101 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
8161 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
8221 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
8281 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
8341 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
8401 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
8461 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
8521 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
8581 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
8641 taacgcttac aatttac
//
